Review
Biochemistry & Molecular Biology
Mariam Elshiaty, Hannah Schindler, Petros Christopoulos
Summary: The field of multispecific therapeutic antibodies is growing rapidly, with over 140 different molecules currently in clinical testing and excellent results in recent clinical trials. Strategies for enhancing anticancer efficacy are focused on disrupting multiple surface antigens and redirecting cytotoxic lymphocytes, with a trend towards more target antigens and increased valency in newer constructs. Exciting prospects include targeting intracellular neoantigens using T-cell receptor fusion proteins or TCR-mimic antibody fragments, offering hope for cure of advanced cancers in the near future.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Nicolas Lambert, Isabelle Hansen, Majdouline El Moussaoui, Jean-Baptiste Giot, Christelle Vercheval, Emilie Lommers, Joan Somja, Michel Moutschen, Pierre Maquet
Summary: This article reports a case of tocilizumab induced sarcoidosis-like reaction, emphasizing the importance of prompt diagnosis and discussing the potential pathophysiological mechanisms underlying this rare reaction.
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2021)
Article
Chemistry, Analytical
Gang Wu, Chuanfei Yu, Wenbo Wang, Jialiang Du, Zhihao Fu, Gangling Xu, Meng Li, Lan Wang
Summary: Imaged capillary isoelectric focusing (icIEF) and ion exchange chromatography (IEX) are routinely used for charge variant analysis of therapeutic monoclonal antibodies (mAbs). In this study, icIEF-MS and strong cation exchange (SCX)-MS were compared, and it was found that icIEF-MS outperformed SCX-MS in terms of sensitivity, carryover effect, protein identification, and separation resolution.
ANALYTICAL CHEMISTRY
(2023)
Article
Medicine, General & Internal
Haoqian Li, Fengchun Mu, Bing Zou, Linlin Wang
Summary: Esophageal cancer is a deadly cancer with high incidence and mortality rates. Immune checkpoint inhibitors (ICIs) have revolutionized cancer treatment but can also lead to immune-related adverse events. This case report describes a patient with esophageal squamous cell carcinoma who developed pulmonary sarcoidosis-like reactions after receiving sintilimab, a PD-1 inhibitor. The patient's symptoms improved with corticosteroid treatment.
Article
Immunology
Yaqiong Chen, Lin Weng, Wei Liu, Chenxi Deng, Jinxiu Xuan, Yuan Ma, Cao Li, Jinlu Jiang, Juan Chen, Shengxiang Ge
Summary: This study successfully developed two monoclonal antibodies specifically recognizing a single citrulline residue, demonstrating higher reactivity, broader detection spectrum, higher specificity, and overall better applicability compared to commercial antibodies.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Ki-Beom Moon, Jae-Heung Jeon, Hyukjun Choi, Ji-Sun Park, Su-Jin Park, Hyo-Jun Lee, Jeong Mee Park, Hye Sun Cho, Jae Sun Moon, Hyunwoo Oh, Sebyung Kang, Hugh S. Mason, Suk-Yoon Kwon, Hyun-Soon Kim
Summary: The global pandemic of SARS-CoV-2 has led to a public health emergency, sparking rapid progress in vaccine development worldwide. Plant-derived SARS-CoV-2 VLPs offer promising platforms for vaccine development, with potential applications to enhance immunogenicity and address mutations in S antigens.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Zicheng Hu, Sivan Cohen, Steven J. Swanson
Summary: This study identified a positive correlation between the clinical ADA rate and the number of introduced mutations in the antibody sequences. The use of rare V alleles in human-origin antibody therapeutics was also found to be associated with higher risk of immunogenicity.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Food Science & Technology
Yanan Wang, Xiaofei Wang, Haitang Zhang, Hanna Fotina, Jinqing Jiang
Summary: This study successfully developed an ELISA method for detecting and monitoring ZENs, prepared high-affinity and broad-specificity mAbs under optimized conditions, and synthesized the ZEN-BSA immunogen and ZEN-OVA coating antigen.
Article
Multidisciplinary Sciences
Emanuele Andreano, Ida Paciello, Giulia Piccini, Noemi Manganaro, Piero Pileri, Inesa Hyseni, Margherita Leonardi, Elisa Pantano, Valentina Abbiento, Linda Benincasa, Ginevra Giglioli, Concetta De Santi, Massimiliano Fabbiani, Ilaria Rancan, Mario Tumbarello, Francesca Montagnani, Claudia Sala, Emanuele Montomoli, Rino Rappuoli
Summary: Research suggests that individuals who have been infected or vaccinated for the first time will produce antibodies with increased potency and breadth, allowing for better control of emerging SARS-CoV-2 variants.
Article
Biochemistry & Molecular Biology
Qi Shao, Shuangjie Li, Qi Cao, Haotian Gu, Jiajia Zhang, Youwen Zhang, Kaili Zhang, Wanglong Zheng, Nanhua Chen, Shaobin Shang, Jianzhong Zhu
Summary: Monoclonal antibodies (mAbs) against porcine RLR proteins were developed, targeting different regions for RIG-I, MDA5, and LGP2. It was found that the RIG-I and MDA5 mAbs were specific to pigs, while the LGP2 mAbs showed reactivity with both pigs and humans. This study not only provides useful tools for porcine RLR antiviral signaling research, but also reveals insights into porcine innate immunity and immune biology by highlighting the porcine species specificity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Fisheries
Zhao Jia, Jianhua Feng, Gaoliang Yuan, Hehe Xiao, Huifeng Dang, Yanwei Zhang, Kangyong Chen, Jun Zou, Junya Wang
Summary: Monoclonal antibodies against grass carp Metrnl-a were prepared and characterized in this study. The expression of CiMetrnl-a increased in grass carp infected with A. hydrophila, particularly in the gills, head kidney and intestine. CiMetrnl-a producing cells were mainly detected in the mucosal layer of the inflamed intestine caused by A. hydrophila infection. qRT-PCR analysis confirmed the marked induction of CiMetrnl-a mRNA expression. These findings suggest the involvement of CiMetrnl-a in regulating bacterial infection-induced intestine inflammation.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Review
Oncology
Jakub Radocha, Niels W. C. J. van de Donk, Katja Weisel
Summary: Monoclonal antibodies have significantly advanced the treatment of multiple myeloma, with anti-CD38 antibodies and SLAM-F7 antibodies showing significant improvements in patient outcomes. Novel monoclonal antibody drug conjugates have also provided hope for refractory multiple myeloma patients.
Article
Biochemistry & Molecular Biology
Cristina Kroon, Larissa Breuer, Lydia Jones, Jeehye An, Ayca Akan, Elkhansa Ahmed Mohamed Ali, Felix Busch, Marinus Fislage, Biswajit Ghosh, Max Hellrigel-Holderbaum, Vartan Kazezian, Alina Koppold, Cesar Alberto Moreira Restrepo, Nico Riedel, Lea Scherschinski, Fernando Raul Urrutia Gonzalez, Tracey L. Weissgerber
Summary: This article examines publication practices of Western blot images and finds that most published Western blots are cropped and source data are not provided to readers. The methods sections of Western blot articles often lack important information. The authors provide detailed descriptions and visual examples, as well as additional resources to help scientists improve Western blot figures and methods.
Article
Chemistry, Multidisciplinary
Tyler D. Moeller, Shivem B. Shah, Kristine Lai, Natalia Lopez-Barbosa, Primit Desai, Weiyao Wang, Zhe Zhong, David Redmond, Matthew P. DeLisa, Ankur Singh
Summary: Using a biomaterials-based B-cell follicular organoid system, glycoengineered bacteria were found to induce the formation of germinal centers and the production of high-affinity antibodies in a significantly shorter time compared to traditional animal immunization-based workflows. These findings demonstrate the potential of synthetic organoids for predicting vaccine efficacy and expediting antibody discovery.
ACS CENTRAL SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Fabiola Vacca, Claudia Sala, Rino Rappuoli
Summary: Monoclonal antibody therapy has significant potential in the pharmaceutical field, particularly in combating antibiotic resistance in bacteria. However, there is a need for further improvement in the efficacy of antibacterial monoclonal antibodies.
Article
Medicine, Research & Experimental
Magfur E. Alam, Gregory V. Barnett, Thomas R. Slaney, Charles G. Starr, Tapan K. Das, Peter M. Tessier
MOLECULAR PHARMACEUTICS
(2019)
Article
Biotechnology & Applied Microbiology
Samuel D. Stimple, Sibel Kalyoncu, Alec A. Desai, Jesper E. Mogensen, Lotte T. Spang, Desiree J. Asgreen, Arne Staby, Peter M. Tessier
BIOTECHNOLOGY AND BIOENGINEERING
(2019)
Article
Engineering, Chemical
Samuel D. Stimple, Matthew D. Smith, Peter M. Tessier
Article
Medicine, Research & Experimental
Yulei Zhang, Lina Wu, Priyanka Gupta, Alec A. Desai, Matthew D. Smith, Lilia A. Rabia, Seth D. Ludwig, Peter M. Tessier
MOLECULAR PHARMACEUTICS
(2020)
Article
Medicine, Research & Experimental
Charles G. Starr, Emily K. Makowski, Lina Wu, Brendan Berg, Jonathan S. Kingsbury, Yatin R. Gokarn, Peter M. Tessier
Summary: CS-SINS is a method that measures colloidal self-interactions at ultradilute antibody concentrations, predicting antibody developability issues at high concentrations, enabling large-scale, high-throughput selection of developable antibodies during early discovery stages.
MOLECULAR PHARMACEUTICS
(2021)
Article
Medicine, Research & Experimental
Priyanka Gupta, Emily K. Makowski, Sandeep Kumar, Yulei Zhang, Justin M. Scheer, Peter M. Tessier
Summary: The study evaluated the repulsive self-interactions and non-specific interactions properties of 42 IgG1 variants, finding that antibodies with the strongest repulsive self-interactions in standard formulations display the strongest non-specific interactions in physiological solution conditions, and vice versa. The best combination was found in antibodies with weakly basic isoelectric points and Fv isoelectric points.
MOLECULAR PHARMACEUTICS
(2022)
Article
Chemistry, Analytical
Weishu Wu, Xiaotian Tan, Jennifer Zupancic, John S. Schardt, Alec A. Desai, Matthew D. Smith, Jie Zhang, Liangzhi Xie, Maung Khaing Oo, Peter M. Tessier, Xudong Fan
Summary: In this study, a rapid and quantitative assay was developed to evaluate the neutralizing efficacy of antibodies and nanobodies against COVID-19. The assay showed comparable results to traditional virus neutralization tests and demonstrated its ability to assess the efficacy against different SARS-CoV-2 variants.
ANALYTICAL CHEMISTRY
(2022)
Review
Biochemical Research Methods
Emily K. Makowski, John S. Schardt, Peter M. Tessier
Summary: Monoclonal antibodies are widely used in the treatment of various human disorders. However, there are challenges in the early stages of antibody drug development, particularly in generating antibodies against membrane proteins and identifying antibodies with drug-like biophysical properties. This article highlights bionanotechnologies for preparing functional membrane proteins and screening antibody biophysical properties, which can improve antibody discovery and engineering efforts.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Harkamal S. Jhajj, Timon S. Lwo, Emily L. Yao, Peter M. Tessier
Summary: Agonist antibodies targeting immune checkpoints, especially those in the TNFR superfamily, are important for cancer treatment by regulating immune cell activity. However, their clinical utility is limited and antibody optimization is urgently needed to improve their therapeutic potential.
TRENDS IN MOLECULAR MEDICINE
(2023)
Review
Chemistry, Multidisciplinary
Ryan Limbocker, Nunilo Cremades, Roberta Cascella, Peter M. Tessier, Michele Vendruscolo, Fabrizio Chiti
Summary: The misfolding and aggregation of peptides and proteins into amyloid aggregates is a common feature of various protein misfolding diseases, including Alzheimer's disease and Parkinson's disease. Misfolded protein oligomers, which can form intermediates in the fibril formation process or be released by mature fibrils, are increasingly recognized as central to the development of these diseases. Despite challenges in studying these oligomers, researchers have developed methods to produce stable and reproducible populations for experimentation. These tools have provided insights into the structural determinants of oligomer toxicity and potential therapeutic strategies.
ACCOUNTS OF CHEMICAL RESEARCH
(2023)
Article
Biochemical Research Methods
Matthew D. Smith, Marshall A. Case, Emily K. Makowski, Peter M. Tessier
Summary: The PSERM scoring method utilizes comprehensive deep sequencing data to score each observed protein variant, providing a more accurate determination of the optimal variants. PSERM scores are more reproducible and correlate stronger with experimentally measured properties compared to frequency or enrichment ratio methods, making it suitable for various protein engineering campaigns.
Article
Biochemical Research Methods
Jennifer M. Zupancic, Alec A. Desai, Peter M. Tessier
Summary: This study presents a simple method to isolate high-affinity nanobodies from common framework libraries using CDR-swapping mutagenesis. By shuffling the CDRs of low-affinity variants during the sorting process, high-affinity nanobodies can be directly isolated without the need for lead isolation and optimization. This approach, demonstrated here for SARS-CoV-2 neutralizing nanobodies, is expected to simplify the generation of high-affinity nanobodies.
Article
Pharmacology & Pharmacy
Jennifer M. Zupancic, John S. Schardt, Alec A. Desai, Emily K. Makowski, Matthew D. Smith, Ghasidit Pornnoppadol, Mayara Garcia de Mattos Barbosa, Marilia Cascalho, Thomas M. Lanigan, Peter M. Tessier
Summary: A novel multivalent nanobody, VHH-72, has shown significant neutralizing activity against various SARS-CoV-2 variants with high biophysical stability, solubility, and specificity.
ADVANCED THERAPEUTICS
(2021)
Review
Medicine, Research & Experimental
Emily K. Makowski, Lina Wu, Priyanka Gupta, Peter M. Tessier
Summary: Developing monoclonal antibodies as therapeutic agents for diverse human disorders has sparked intense interest. Key recent advances in high-throughput methods for identifying antibodies with desirable properties have shown great promise in rational antibody design and prediction of drug-like behaviors, but outstanding challenges remain in fully realizing their potential to minimize development times and improve success rates in the clinic.
Article
Medicine, Research & Experimental
Emily K. Makowski, Lina Wu, Alec A. Desai, Peter M. Tessier
Summary: The PolySpecificity Particle (PSP) assay is a sensitive flow cytometry method for evaluating antibody nonspecific interactions, overcoming previous limitations and allowing for evaluation of diverse types of antibodies. This assay is more sensitive than standard assays like ELISAs, and has the potential to accelerate the development of safe and effective antibody therapeutics.
Review
Biochemical Research Methods
Pei-Ru Chen, Peng-Fei Xia
Summary: Carbon dioxide plays a crucial role in mitigating global climate change and supporting life on Earth. Synthetic biology and metabolic engineering offer a promising approach to enhance CO2 fixation by combining the advantages of autotrophic and heterotrophic organisms. This article reviews the current progress in constructing synthetic CO2 fixation pathways, discusses the underlying design principles and challenges, and provides insights into the future of carbon recycling through engineered synthetic carbon fixation pathways.
CURRENT OPINION IN BIOTECHNOLOGY
(2024)
