Journal
CLINICAL NUTRITION
Volume 39, Issue 11, Pages 3377-3384Publisher
CHURCHILL LIVINGSTONE
DOI: 10.1016/j.clnu.2020.02.027
Keywords
Circulating microvesicles; Mediterranean diet; Cardiovascular disease; Platelets; Leukocytes; Cell activation
Categories
Funding
- Spanish Ministry of Economy and Competitiveness of Science [SAF2016-76819-R]
- Institute of Health Carlos III, ISCIII [TERCEL - RD16/0011/0018, CIBERCV CB16/11/0041]
- Fundacio Marato TV3 [PI0444003]
- FEDER Una Manera de Hacer Europa
- CERCA Programme/Generalitat de Catalunya Spain
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Background & aims: Circulating microvesicles (cMV) are small phospholipid-rich vesicles that contribute to the atherothrombotic process, and are biomarkers of cardiovascular disease (CVD) burden and progression. Diet is a cornerstone for CVD prevention, but dietary effects on cMV shedding are poorly characterized. We aimed at assessing the long term effects of a Mediterranean diet compared to a low-fat diet (LFD) on MV shedding by cells of the blood and vascular compartments in patients at high cardiovascular risk treated as per guidelines. Methods: A total of 155 participants from the PREDIMED trial free of cardiovascular events after a mean follow-up of 5 years (n = 53 from the Mediterranean diet supplemented with extra-virgin olive oil-EVOO-; n = 49 from the Mediterranean diet supplemented with mixed nuts -Nuts-; and n = 53 from the LFD) were included in the study. At baseline and after one-year intervention, cMV were quantified and characterized by flow cytometry to identify their activated parental cell origin and prothrombotic potential by Annexin V (AV) binding. Results: After one year of dietary intervention, platelet-derived PAC-1(+)/AV(+) and CD62P(+)/AV(+) cMV concentrations were lower in the Nuts group compared with the LFD and EVOO interventions (P = 0.036 and 0.003, respectively). In addition, prothrombotic cMV carrying tissue factor (CD142(+)/AV(+)) and CD11a(+) /AV(+) cMV derived from activated cells, were significantly lower in both Mediterranean diet (EVOO and Nuts) interventions compared to one year of LFD (P < 0.0001 and 0.028, respectively). SMA alpha(+)/AV(+) cMV were lower in the LFD compared to the Nuts group after one year of intervention (P = 0.038). Conclusions: cMV are markers of cell activation and vascular injury that appear to be sensitive to dietary changes. Following a Mediterranean diet rich in EVOO or nuts is associated with lower cell activation towards a pro-atherothrombotic phenotype, suggesting a delay in the development of CV complications. (C) 2020 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.
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