Article
Chemistry, Multidisciplinary
Jing Tu, Hao Meng, Linlin Wu, Guohao Xi, Jiye Fu, Zuhong Lu
Summary: EasyNanopore is a user-friendly software designed for selecting events in nanopore molecular translocation experiments. It utilizes an adaptive threshold and multiprocess method to quickly extract data from large-scale experiments and generate result txt files for further analysis.
Article
Biochemistry & Molecular Biology
Maria Pilar Giner, Stefan Christen, Simona Bartova, Mikhail Makarov, Marie E. Migaud, Carles Canto, Sofia Moco
Summary: NAD(+) and its reduced form NADH are essential coenzymes in hundreds of metabolic reactions, serving as substrates for key enzymes. Analyzing the NAD(+) metabolome using liquid chromatography-tandem mass spectrometry can provide insights into NAD(+) biology and its implications for health and disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemical Research Methods
Congrou Zhang, Sylvia E. Le Devedec, Ahmed Ali, Thomas Hankemeier
Summary: Single-cell metabolomics (SCMs) is a powerful tool for studying cellular heterogeneity by providing insight into the differences between individual cells. With the development of a set of promising SCMs pipelines, this maturing technology is expected to be widely used in biomedical research. However, before SCMs is ready for primetime, there are some challenges to overcome. In this review, we summarize the trends and challenges in the development of SCMs and highlight the latest methodologies, applications, and integration perspectives with other omics and imaging approaches.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Nutrition & Dietetics
Sarah Brajkovic, Nils Rugen, Carlos Agius, Nicola Berner, Stephan Eckert, Amirhossein Sakhteman, Claus Schwechheimer, Bernhard Kuster
Summary: Plants are crucial for sustainable global food supply, but the understanding of crop proteomes is limited. The presence of secondary metabolites in crop plants has hindered proteomic analyses, requiring individually optimized protein extraction protocols. In this study, a universal protein extraction protocol combined with an automated SP3 protocol and optimized micro-LC-MS/MS conditions was developed for high-quality proteomic analysis of crop plants. The workflow was successfully applied to analyze the proteomes of mature tomato fruits, demonstrating its robustness and potential for large-scale projects mapping crop tissue proteomes.
Article
Biochemical Research Methods
Rosa Pilolli, Antonella Lamonaca, Chiara Nitride, Elisabetta De Angelis, Christof van Poucke, Nathalie Gillard, Anne-Catherine Huet, Marc De Loose, Jean Henrottin, E. C. N. Mills, Linda Monaci
Summary: Mass spectrometry has been widely used for the sensitive detection of undeclared allergenic ingredients. In this study, a quantitative method was developed and validated for the determination of six allergenic ingredients in chocolate. The method showed good sensitivity and compliance with the current threshold doses.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Nicole Strittmatter, Frances M. Richards, Alan M. Race, Stephanie Ling, Daniel Sutton, Anna Nilsson, Yann Wallez, Jennifer Barnes, Gareth Maglennon, Aarthi Gopinathan, Rebecca Brais, Edmond Wong, Maria Paola Serra, James Atkinson, Aaron Smith, Joanne Wilson, Gregory Hamm, Timothy Johnson, Charles R. Dunlop, Brajesh P. Kaistha, Josephine Bunch, Owen J. Sansom, Zoltan Takats, Per E. Andren, Alan Lau, Simon T. Barry, Richard J. A. Goodwin, Duncan Jodrell
Summary: Gemcitabine distribution and metabolism were assessed using a multimodal imaging approach, revealing that active metabolites primarily reached tumor cell compartments, while an ATR inhibitor targeted nonviable tumor regions.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Sanaz C. Habibi, Gabe Nagy
Summary: In recent years, ion mobility spectrometry-mass spectrometry (IMS-MS) has advanced omics-based research with high-resolution platforms, but most separations have been qualitative. The rotational averaged ion neutral collision cross section (CCS) is a quantitative metric for characterizing biomolecules in IMS-MS. Determining CCS in multipass IMS systems has been challenging due to a lack of methods for calculating CCS and the difficulty of requiring identical passes for calibrants and unknown compounds. This study presents a method using average ion velocities to calculate CCS values in cyclic IMS-MS separations, demonstrating good agreement with previous literature and potential applicability to other IMS-MS platforms.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Bin Zhang, Deling Shi, Mengmeng Li, Feng Shi, Lianli Chi
Summary: Heparin is a widely used anticoagulant extracted from animal tissues, with porcine intestine mucosa being the main legal source. Differentiating heparins derived from porcine, ovine, and bovine is challenging, especially when ruminant heparin is adulterated into porcine heparin. This study establishes a quantitative mass spectrometry method to selectively monitor marker saccharides, allowing for the differentiation of porcine heparin adulterated with low amounts of ruminant heparin, ensuring the authenticity and safety of heparin drugs.
CARBOHYDRATE POLYMERS
(2023)
Article
Chemistry, Analytical
Nuria Fontanals, Eva Pocurull, Rosa Montes, Iria Gonzalez-Marino, Sergio Santana-Viera, Manuel Miro, Andreu Rico, Sara Rodriguez-Mozaz, Francesc Borrull, Jose Benito Quintana, Rosa Maria Marce
Summary: This study describes an analytical method based on solid-phase extraction and liquid chromatography coupled to tandem mass spectrometry to monitor the occurrence of a group of seventeen pharmaceuticals in wastewater. The results showed that all studied pharmaceuticals were found in all samples, with concentrations ranging from non-detectable to 10,393 ng/L, and the highest concentrations were for tramadol. Furthermore, the study also found that the pattern of pharmaceutical use was generally similar in all cities monitored.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemical Research Methods
Roger Gine, Jordi CapeHades, Josep M. Badia, Dennis Vughs, Michaela Schwaiger-Haber, Theodore Alexandrov, Maria Vinaixa, Andrea M. Brunner, Gary J. Patti, Oscar Yanes
Summary: HERMES is a molecular-formula-oriented and peak-detection-free method that uses LC/MS1 information to optimize MS2 acquisition, improving the identification rate and biological specificity of metabolites.
Article
Environmental Sciences
Laura Rohler, Pernilla Bohlin-Nizzetto, Pawel Rostkowski, Roland Kallenborn, Martin Schlabach
Summary: Long-term monitoring of regulated organic chemicals in ambient air provides valuable information about their environmental fate and trends, essential for evaluating regulatory effectiveness. New suspect and non-target screening analytical strategies are effective in identifying emerging compounds.
ATMOSPHERIC CHEMISTRY AND PHYSICS
(2021)
Article
Chemistry, Applied
Ines Rodriguez-Canas, Jesus M. Gonzalez-Jartin, Amparo Alfonsoa, Rebeca Alvarino, Mercedes R. Vieytes, Luis M. Botana
Summary: Plant-based beverages have become popular in recent years due to environmental and ethical concerns, as well as milk intolerances and allergies. However, the raw materials used to make these beverages are susceptible to mycotoxin contamination. Researchers have developed a new method for analyzing mycotoxins in oat, rice, soy, and almond beverages, and found that several types of mycotoxins are present in these products.
Article
Food Science & Technology
Marion Daniela Marchetti, Alejandra Tomac, Maria Isabel Yeannes, Analia Belen Garcia Loredo
Summary: Changes in consumer lifestyle and demand for healthier food have led to a decline in salted fish consumption. This study evaluated the use of vacuum technology in desalting hake to develop a ready-to-use product. The results showed that vacuum technology reduced desalting time and improved sensory acceptability of the product.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Review
Oncology
Rachel J. Keogh, John C. Riches
Summary: Breath analysis is a promising non-invasive method for detecting and managing lung cancer. It involves analyzing the complex mixture of compounds in exhaled breath to identify unique breath signatures associated with lung cancer. Gas chromatography, mass spectrometry, and gaseous chemical sensing devices have been used to detect these signatures. Additionally, specific genomic aberrations in exhaled breath have been explored for molecular analysis. However, methodological issues, lack of standardization, and limited large-scale studies have restricted the use of breath analysis to translational research.
Article
Endocrinology & Metabolism
Naila Celeste Bailey, Jeannett Dimsits, Mette Hammer, David M. Kendall, Timothy S. Bailey
Summary: In the study, it was found that trained caregivers and untrained bystanders were able to administer the dasiglucagon autoinjector more rapidly and with greater ease compared to the Eli Lilly GEK, resulting in higher user satisfaction.
DIABETES TECHNOLOGY & THERAPEUTICS
(2022)
Review
Endocrinology & Metabolism
Samuel D. Vasikaran, Masakazu Miura, Richard Pikner, Harjit P. Bhattoa, Etienne Cavalier
Summary: Bone turnover markers play an important role in the study of osteoporosis medication and are increasingly used in the clinical management of osteoporosis and other metabolic bone diseases. Specific enzymes in the blood can provide reliable measures of bone turnover, regardless of biological variation. Proper patient preparation and sample handling are crucial for accurate measurements.
CALCIFIED TISSUE INTERNATIONAL
(2023)
Review
Endocrinology & Metabolism
Aurelie Ladang, Frank Rauch, Edgard Delvin, Etienne Cavalier
Summary: Bone turnover markers (BTMs) in children and adolescents show age- and gender-dependent variations under pathological conditions. However, due to the specificities of pediatric patients, the clinical use of BTMs is still limited in practice.
CALCIFIED TISSUE INTERNATIONAL
(2023)
Article
Medical Laboratory Technology
Pierre Delanaye, Elke Schaeffner, Mario Cozzolino, Michel Langlois, Mario Plebani, Tomris Ozben, Etienne Cavalier
Summary: The EFLM recommends European laboratories to continue using the 2009 version of the CKD-EPI equation without race correction factor. The new EKFC equation, developed from European cohorts, may serve as an alternative equation for estimating GFR in Europe. Estimating equations based on cystatin C may gain popularity due to their independence of race. Reference methods like iohexol clearance for measuring GFR remain necessary in specific patients and/or situations.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Letter
Medical Laboratory Technology
Guillaume Resimont, Etienne Cavalier, Pierre Delanaye
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Candice Bancal, Florian Salipante, Nassim Hannas, Serge Lumbroso, Etienne Cavalier, David-Paul De Brauwere
Summary: This study compared total and corrected calcium with ionized calcium and developed a machine learning algorithm to predict calcium status. The results showed that corrected calcium performed well in hypocalcemic samples, while total calcium performed better in hypercalcemic and normocalcemic samples. Corrected calcium had issues in hypoalbuminemia, acid-base disorders, renal insufficiency, hyper-phosphatemia, or inflammatory syndrome. The machine learning algorithm achieved 81% correct classifications and provided a better assessment of calcium status compared to total calcium. Ionized calcium assay should be performed if there is doubt.
CLINICA CHIMICA ACTA
(2023)
Article
Medical Laboratory Technology
Pierre Delanaye, Antoine Lanot, Antoine Bouquegneau, Xavier Warling, Luc Radermacher, Catherine Masset, Jean-Marie Krzesinski, Olivier Moranne, Etienne Cavalier
Summary: This study aimed to investigate the necessity of monitoring 1,25-dihydroxyvitamin D levels in hemodialysis patients. The results showed that there was a significant increase in 25-hydroxyvitamin D levels in patients treated with native vitamin D, but no significant change in 1,25-dihydroxyvitamin D levels. For patients treated with active vitamin D, there were no significant changes in both 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D levels. Therefore, monitoring 1,25-dihydroxyvitamin D levels in patients newly treated with active vitamin D does not seem useful.
CLINICA CHIMICA ACTA
(2023)
Letter
Endocrinology & Metabolism
Hanne Skou Jorgensen, Pieter Evenepoel, Hirotaka Komaba, Sandro Mazzaferro, Marc Vervloet, Etienne Cavalier, Masafumi Fukagawa
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Article
Medicine, General & Internal
H. Pottel, J. Bjork, A. D. Rule, N. Ebert, B. O. Eriksen, L. Dubourg, E. Vidal-Petiot, A. Grubb, M. Hansson, E. J. Lamb, K. Littmann, C. Mariat, T. Melsom, E. Schaeffner, P. -O. Sundin, A. Akesson, A. Larsson, E. Cavalier, J. B. Bukabau, E. K. Sumaili, E. Yayo, D. Monnet, M. Flamant, U. Nyman, P. Delanaye
Summary: The study developed a cystatin C-based equation (EKFC eGFRcys) to estimate glomerular filtration rate (GFR). This equation had similar accuracy to a creatinine-based equation (EKFC eGFRcr) and outperformed other commonly used equations. Furthermore, taking the arithmetic mean of EKFC eGFRcr and EKFC eGFRcys further improved the accuracy of GFR estimation.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Biochemical Research Methods
Justine J. Demeuse, Chiara Calaprice, Loreen C. Huyghebaert, Marwa Rechchad, Stephanie Peeters, Etienne Cavalier, Caroline Le Goff
Summary: A growing body of literature suggests that circadian disruption may have significant effects on human health, potentially contributing to psychiatric diseases, metabolic syndrome, and cancers. In order to assess circadian rhythm, the measurement of melatonin levels is considered the best practice. This study developed and validated a highly sensitive LC-MS/MS method to track salivary melatonin levels throughout the day and night, establishing a lower limit of the measuring interval (LLMI) of 0.8 pg/mL. The method was validated according to EMA guidelines and showed accurate, precise, and specific results.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Biochemical Research Methods
Justine Demeuse, Philippe Massonnet, Matthieu Schoumacher, Elodie Grifnee, Loreen Huyghebaert, Thomas Dubrowski, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: In this study, a mass spectrometry-based workflow was proposed to identify new cathepsin K cleavage sites. The results revealed multiple cleavage hotspots and a preference for cleavage at the ends of type I collagen. The study also found that cathepsin K preferentially cleaves amino acid residues with long and hydrophobic lateral chains at the beginning of digestion.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Medicine, General & Internal
Karel David, Gaetan Devos, Nick Narinx, Leen Antonio, Wout Devlies, Ludo Deboel, Dieter Schollaert, Anton Eisenhauer, Etienne Cavalier, Dirk Vanderschueren, Frank Claessens, Steven Joniau, Brigitte Decallonne
Summary: Prostate cancer patients treated with androgen deprivation therapy (ADT) have an increased risk of bone loss. This study suggests that 12 weeks of ADT in non-metastatic PCa patients results in early bone loss, and additional treatment with ARSI does not seem to worsen bone loss in the early phase. Future studies should investigate if these early biomarkers can predict fracture risk and be used in clinical practice for monitoring bone health in PCa patients undergoing ADT.
Review
Endocrinology & Metabolism
Konstantinos Makris, Chagigia Mousa, Etienne Cavalier
Summary: Alkaline phosphatases are isoenzymes that catalyze the hydrolysis of organic phosphate esters in the extracellular space. Zinc and magnesium are important co-factors for their biological activity. They are found in various tissues, but most are derived from the liver and bone in the bloodstream. However, their exact physiological function remains largely unknown.
CALCIFIED TISSUE INTERNATIONAL
(2023)
Letter
Medical Laboratory Technology
Etienne Cavalier, Jordi Farre-Segura
CLINICAL CHEMISTRY
(2023)
Article
Agriculture, Dairy & Animal Science
Patrice Dufour, Vincent Frisee, Goulven Rigaux, Flore Brutinel, Sophie Egyptien, Philippe Bossaert, Jessica Deleersnyder, Stefan Deleuze, Stephanie Peeters, Caroline Le Goff, Jerome Ponthier, Etienne Cavalier
Summary: This report describes the method of measuring progesterone and estrone-sulfate using Liquid Chromatography coupled with Mass Spectrometry in American Bison feces. This method provides an alternative to serum assay for studying the pregnancy-related evolution of these hormones in American Bison.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2024)
Review
Endocrinology & Metabolism
Annemieke C. Heijboer, Etienne Cavalier
Summary: The hormone FGF23 plays a central role in regulating phosphate homeostasis and has been associated with certain diseases. Various immunoassays have been developed to measure FGF23 in blood, but standardization is lacking. Currently, FGF23 measurement is clinically used in the diagnosis of hereditary hypophosphatemic rickets and tumor-induced osteomalacia. However, its usefulness in predicting risks or as a treatment target in CKD patients requires further research.
CALCIFIED TISSUE INTERNATIONAL
(2023)
