Article
Biochemistry & Molecular Biology
Miriam Carbonaro, Martina Aulitto, Giovanni Gallo, Patrizia Contursi, Danila Limauro, Gabriella Fiorentino
Summary: A newly discovered enzyme, AmCel9, from a thermophilic microorganism was found to have broad substrate specificity and good catalytic activity, making it a promising biocatalyst for lignocellulose deconstruction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Catia Santos-Pereira, Joana Sousa, angela M. A. Costa, Andreia O. Santos, Teresa Rito, Pedro Soares, Ricardo Franco-Duarte, Sara C. Silverio, Ligia R. Rodrigues
Summary: In this study, compost samples from three Portuguese companies were analyzed to identify and characterize carbohydrate-active enzymes (CAZymes) and microbial communities. The results showed that the compost samples were rich in glycoside hydrolases (GH), especially cellulases and oligosaccharide-degrading enzymes. The microbiome of the samples was dominated by bacteria, with the classes Gammaproteobacteria, Alphaproteobacteria, and Balneolia being the most abundant. Additionally, comparison with other samples indicated that composting is an excellent source of lignocellulose-degrading enzymes. This is the first comparative study on the abundance and taxonomic/functional profiles of CAZymes in Portuguese compost samples.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
J. Ashwini John, Ethiraj Selvarajan
Summary: This study discusses the conversion of lignocellulosic waste to biofuel using cellulose in rice husks by lichen-associated Streptomyces sp. MS2A. The genome analysis of Streptomyces sp. MS2A confirmed the presence of cellulose-degrading genes and revealed the similarity with Streptomyces sp. CCM_MD2014. The analysis also identified a variety of GH families involved in lignocellulose degradation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Flora Salzano, Martina Aulitto, Gabriella Fiorentino, Emilia Pedone, Patrizia Contursi, Danila Limauro
Summary: A thermo-acidophilic bacterium, Alicyclobacillus mali FL18, was isolated from a hot spring in Italy, and a novel putative beta-xylosidase, Am beta Xyl, belonging to the glycosyl hydrolase family 3, was identified. The enzyme showed high stability and catalytic activity at high temperatures and acidic conditions, and exhibited excellent tolerance to organic solvents and monosaccharides. These characteristics suggest potential applications of Am beta Xyl in various industrial fields.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
Peiqian Zhang, Rongxin Su, Yuhao Duan, Mei Cui, Renliang Huang, Wei Qi, Zhimin He, Wim Thielemans
Summary: The effective binding between cellulases and cellulose is essential for the enzymatic hydrolysis of lignocellulose. Expansin can enhance the efficiency of cellulase by loosening the cellulose structure, and when combined, the hydrolysis rate of cellulose can increase significantly compared to using cellulase alone. These findings provide insights into the real-time synergy between cellulases and expansin on cellulose, and the impact of their synergy on the enzymatic hydrolysis of cellulose.
CARBOHYDRATE POLYMERS
(2021)
Article
Microbiology
Jesus Castano, Jiwei Zhang, Mowei Zhou, Chia-Feng Tsai, Joon Yong Lee, Carrie Nicora, Jonathan Schilling
Summary: The study revealed that side chain hemicellulases in brown rot fungi are more tolerant of ROS compared to white and soft rot fungi, which may have been crucial for the evolution of brown rot fungi.
Article
Biochemistry & Molecular Biology
Jose Antonio Curiel, Angela Peiroten, Jose Maria Landete, Ana Ruiz de la Bastida, Susana Langa, Juan Luis Arques
Summary: This study examines the relationship between fucosylated carbohydrates and glycoproteins in human breast milk and the gut microbiota, particularly bifidobacteria. The research identifies alpha-L-fucosidases from bifidobacteria, which play a key role in the utilization of these carbohydrates, and classifies them into three families based on catalytic structure. Interestingly, bifidobacterial alpha-L-fucosidases within the same family show significant differences in sequences, suggesting possible distinct phylogenetic evolution. Additionally, the study reveals multiple phylogenetic lineages of fucosidases in Bifidobacterium longum subsp. infantis species, indicating potential applications in synthesizing novel prebiotics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Agricultural Engineering
Hongliang Guo, Ying Zhao, Jo-Shu Chang, Duu-Jong Lee
Summary: Enzymatic hydrolysis is crucial for efficient biorefinery of lignocellulosic biomass. Understanding the properties of key enzymes and their interactions with biomass is essential for improving the process efficiency. This article provides an update on the research of key enzymes in lignocellulose biorefinery, including their properties, interactions with biomass, adsorption, and hydrolysis. The advanced analytical techniques for investigating these interactions and future research prospects are discussed.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Mareike Monschein, Eleni Ioannou, Taru Koitto, Leamon A. K. M. Al Amin, Jutta J. Varis, Edward R. Wagner, Kirsi S. Mikkonen, Daniel J. Cosgrove, Emma R. Master
Summary: This study investigates microbial expansin-related proteins and their applied significance by comparing diverse loosenins encoded by the same organism, considering both cellulose and chitin-containing materials as targeted substrates, and investigating the impact of the C-terminal carbohydrate binding module (CBM) on loosenin function. The study reveals the potential of fungal loosenins to impact both cellulose and chitin-containing networks, highlighting their possible biological and applied role in fungal cell wall processing.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Plant Sciences
Nestor Walter Soria, Maria Soledad Diaz, Ana Cristina Figueroa, Valeria Roxana Alasino, Pablo Yang, Dante Miguel Beltramo
Summary: Chitin is a major component of the fungal cell wall, with synthesis by Chitin synthases and degradation by chitinases. The phytopathogenic fungus Thecaphora frezii contains eight Chitin Synthases and four Chitinases, showing similarities with Ustilago maydis. This study contributes to a better understanding of the pathogen's biology through enzyme expression analysis and chitinase activity evaluation.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2021)
Review
Food Science & Technology
Vidhya K. Shinde, Koteswara Rao Vamkudoth
Summary: Oligosaccharides, with their excellent bio-preservative and prebiotic properties, have gained popularity among modern consumers, leading to increased market demand. Innovative and cost-effective technologies for efficient oligosaccharides synthesis, including genetic engineering and reaction optimization, are crucial for meeting the growing demand in the food, pharmaceuticals, and cosmetic industry.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2022)
Review
Biotechnology & Applied Microbiology
Murugan Kumar, Hillol Chakdar, Kuppusamy Pandiyan, Shobit Thapa, Mohammad Shahid, Arjun Singh, Alok Kumar Srivastava, Anil Kumar Saxena
Summary: Chitinases are enzymes that catalyze the hydrolysis of chitin and are present in all domains of life. Bacterial chitinases are of particular interest due to their widespread distribution and ability to function under extreme conditions. They have important applications in agriculture and industry, such as controlling pests and pathogens, waste management, and synthesis of bioactive compounds.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Agricultural Engineering
Chengbao Xu, Tao Xia, Hao Peng, Peng Liu, Yihong Wang, Yanting Wang, Heng Kang, Jingfeng Tang, Muhammad Nauman Aftab, Liangcai Peng
Summary: In this study, the BsEXLE1 gene was overexpressed in T. reesei to create the TrEXLX10 strain, which showed increased activities of beta-glucosidases, cellobiohydrolases, and xylanses compared to the Rut-C30 strain. The enzymes secreted by TrEXLX10 resulted in higher yields of hexoses in biomass saccharification experiments. Furthermore, the study identified the potential of expansin, purified from the TrEXLX10 secreted solution, to enhance cellulose hydrolysis independently.
BIORESOURCE TECHNOLOGY
(2023)
Article
Microbiology
Yuan Qu, Yuan Luo, Xulei Yang, Yu Zhang, En Yang, Huini Xu, Yingying He, Irbis Chagan, JinPing Yan
Summary: In this study, two GH3 gene family members of Trametes trogii were cloned and characterized. One of the enzymes, TtBgl3, showed beta-glucosidase activity towards phenolic glycosides. The optimal pH and stability range of TtBgl3 were determined, and it was found to have dual activity towards aryl-beta-D-glucosidase and beta-glucuronidase. Point mutation analysis revealed potential active sites in TtBgl3. These findings provide insights for the identification of new GH3 beta-glucosidases and the application of TtBgl3 as a biological catalyst.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Katesuda Aiewviriyasakul, Benjarat Bunterngsook, Hataikarn Lekakarn, Wipawee Sritusnee, Pattanop Kanokratana, Verawat Champreda
Summary: The study developed an endo-beta-1,4-xylanase with high specificity for producing prebiotic xylooligosaccharides, operating optimally at moderate temperatures to reduce energy costs in production processes. The enzyme showed potential for XOS production and lignocellulosic biomass saccharification, working best at 45 degrees Celsius and pH 6.0 with specific cleavage patterns releasing xylobiose as the major product.
BIOTECHNOLOGY LETTERS
(2021)
Article
Biotechnology & Applied Microbiology
Anchanee Kubera, Arinthip Thamchaipenet, Menachem Shoham
Article
Biotechnology & Applied Microbiology
Shigeru Kitani, Tatsuya Ueguchi, Yasuhiro Igarashi, Kantinan Leetanasaksakul, Arinthip Thamchaipenet, Takuya Nihira
JOURNAL OF ANTIBIOTICS
(2018)
Article
Microbiology
Hathairat Rachniyom, Atsuko Matsumoto, Yuki Inahashi, Akira Take, Yoko Takahashi, Arinthip Thamchaipenet
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2018)
Article
Plant Sciences
Hirofumi Akiyama, Chantra Indananda, Arinthip Thamchaipenet, Atsuko Motojima, Tsutomu Oikawa, Hisayuki Komaki, Akira Hosoyama, Akane Kimura, Naoya Oku, Yasuhiro Igarashi
JOURNAL OF NATURAL PRODUCTS
(2018)
Article
Multidisciplinary Sciences
Ratchaniwan Jaemsaeng, Chatchawan Jantasuriyarat, Arinthip Thamchaipenet
SCIENTIFIC REPORTS
(2018)
Article
Biochemical Research Methods
Daniel P. Martins, Kantinan Leetanasaksakul, Michael Taynnan Barros, Arinthip Thamchaipenet, William Donnelly, Sasitharan Balasubramaniam
IEEE TRANSACTIONS ON NANOBIOSCIENCE
(2018)
Article
Environmental Sciences
Onsiri Siripan, Arinthip Thamchaipenet, Wunrada Surat
INTERNATIONAL JOURNAL OF PHYTOREMEDIATION
(2018)
Article
Multidisciplinary Sciences
Suranan Yoolong, Worarat Kruasuwan, Huyen Thi Thanh Pham, Ratchaniwan Jaemsaeng, Chatchawan Jantasuriyarat, Arinthip Thamchaipenet
SCIENTIFIC REPORTS
(2019)
Article
Microbiology
Supattra Muangham, Kenika Lipun, Arinthip Thamchaipenet, Atsuko Matsumoto, Kannika Duangmal
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2019)
Article
Plant Sciences
Wilaiwan Koomsiri, Yuki Inahashi, Kantinan Leetanasaksakul, Kazuro Shiomi, Yoko Takahashi, Satoshi Omura, Markiyan Samborskyy, Peter F. Leadlay, Pakorn Wattana-Amorn, Arinthip Thamchaipenet, Takuji Nakashima
JOURNAL OF NATURAL PRODUCTS
(2019)
Article
Microbiology
Huyen Thi Thanh Pham, Wipawadee Suwannapan, Wilaiwan Koomsiri, Yuki Inahashi, Akira Take, Atsuko Matsumoto, Arinthip Thamchaipenet
Article
Plant Sciences
Chakrit Bunyoo, Peerapat Roongsattham, Sirikorn Khumwan, Juthaporn Phonmakham, Passorn Wonnapinij, Arinthip Thamchaipenet
Summary: This study investigated the structure and diversity of duckweed-associated bacteria (DAB) among four duckweed subtypes under natural and nutrient-deficient conditions using 16S rRNA amplicon sequencing. The results showed that Proteobacteria was the dominant phylum in duckweed samples. A total of 24 microbial genera were identified as a core microbiome, with consistent proportions across all duckweed subtypes. The diversity of microbial communities decreased after nutrient-deficient stress, but the relative abundance of certain genera significantly increased. Functional prediction of the metagenome data revealed enrichment of essential pathways involved in DAB colonization and biodegradation under stress condition.
Review
Plant Sciences
Manik Prabhu Narsing Rao, Karan Lohmaneeratana, Chakrit Bunyoo, Arinthip Thamchaipenet
Summary: This article discusses the impact of abiotic stress on plant-associated actinobacterial diversity and actinobacteria-mediated stress mitigation processes. Multi-omics techniques play a crucial role in expanding plant-actinobacteria interactions, aiding plants in overcoming abiotic stresses.
Article
Veterinary Sciences
Teerasak E-kobon, Ratiporn Leeanan, Saengtian Pannoi, Pornchai Anuntasomboon, Pacharee Thongkamkoon, Arinthip Thamchaipenet
BMC VETERINARY RESEARCH
(2017)
Article
Microbiology
Worarat Kruasuwan, Paul A. Hoskisson, Arinthip Thamchaipenet
GENOME ANNOUNCEMENTS
(2017)
Article
Agricultural Engineering
Yinping Xiang, Meiying Jia, Rui Xu, Jialu Xu, Lele He, Haihao Peng, Weimin Sun, Dongbo Wang, Weiping Xiong, Zhaohui Yang
Summary: This study investigated the impact of the non-antibiotic pharmaceutical carbamazepine on antibiotic resistance genes (ARGs) during anaerobic digestion. The results showed that carbamazepine induced the enrichment of ARGs and increased the abundance of bacteria carrying these genes. It also facilitated microbial aggregation and intercellular communication, leading to an increased frequency of ARGs transmission. Moreover, carbamazepine promoted the acquisition of ARGs by pathogens and elevated their overall abundance.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Weixin Zhao, Tianyi Hu, Hao Ma, Dan Li, Qingliang Zhao, Junqiu Jiang, Liangliang Wei
Summary: This review summarizes the effects and potential mechanisms of biochar on microbial behavior in AD systems. The addition of biochar has been found to promote microbial colonization, alleviate stress, provide nutrients, and enhance enzyme activity. Future research directions include targeted design of biochar, in-depth study of microbial mechanisms, and improved models.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Christina Karmann, Anna Magrova, Pavel Jenicek, Jan Bartacek, Vojtech Kouba
Summary: This review assesses nitrogen removal technologies in reject water treatment, highlighting the differences in environmental impacts and economic benefits. Partial nitritation-anammox shows potential for economic benefits and positive environmental outcomes when operated and controlled properly.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Wei-Hao Huang, Ying-Ju Chang, Duu-Jong Lee
Summary: This study modified pinecone biochar with layered double hydroxide (LDH) to enhance its adsorption capacity for heavy metal and phosphate ions. The LDH-biochar showed significantly improved adsorption capacities for Pb2+ and phosphate, and a slight increase for Cu2+ and Co2+. The LDH layer enhanced the adsorption through various mechanisms.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen
Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sang Min Lee, Ju Young Lee, Ji-Sook Hahn, Seung-Ho Baek
Summary: This study successfully developed an efficient platform strain using Yarrowia lipolytica for the bioconversion of renewable resources into adipic acid, achieving a remarkable increase in production level.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Sefkan Kendir, Matthias Franzreb
Summary: This study presents a novel approach using magnetic separation to efficiently harvest freshwater microalgae, Chlorella vulgaris. By combining pH-induced calcium phosphate precipitation with cheap natural magnetite microparticles, harvesting efficiencies up to 98% were achieved in the model medium.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ishaq Kariim, Ji-Yeon Park, Wajahat Waheed Kazmi, Hulda Swai, In-Gu Lee, Thomas Kivevele
Summary: The impact of reaction temperature, residence time, and ethanol: acetone on the energy compositions and yield enhancement of biocrudes was investigated. The results showed that under appropriate conditions, biocrudes with high energy and low oxygen content can be obtained, indicating a high potential for utilization.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xu Yang, Mahmoud Mazarji, Mengtong Li, Aohua Li, Ronghua Li, Zengqiang Zhang, Junting Pan
Summary: This study investigated the impact of magnetite on the nitrogen cycle of pig manure biostabilisation. The addition of magnetite increased N2O emissions and decreased NH3 emissions during composting. It also increased the total nitrogen content but should be considered for its significant increase in N2O emissions in engineering practice.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Ty Shitanaka, Haylee Fujioka, Muzammil Khan, Manpreet Kaur, Zhi-Yan Du, Samir Kumar Khanal
Summary: The market value of microalgae has exponentially increased in the past two decades, thanks to their applications in various industries. However, the supply of high-value microalgal bioproducts is limited due to several factors, and strategies are being explored to overcome these limitations and improve microalgae production, thus increasing the availability of algal-derived bioproducts in the market.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Martin Kerner, Thorsten Wolff, Torsten Brinkmann
Summary: The efficiency of using enriched CO2 from flue gas for large-scale production of green microalgae has been studied. The results show that the use of membrane devices and static mixers can effectively improve the CO2 recovery rate and maintain the suitable pH and temperature during cultivation, achieving a more economical and sustainable microalgae production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rui Ma, Ji Li, Rd Tyagi, Xiaolei Zhang
Summary: This review summarizes the microorganisms capable of using CO2 and CH4 to produce PHAs, illustrating the production process, factors influencing it, and discussing optimization techniques. It identifies the challenges and future prospects for developing economically viable PHAs production using GHGs as a carbon source.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Bing Wang, Peng Zhang, Xu Guo, Xu Bao, Junjie Tian, Guomin Li, Jian Zhang
Summary: The addition of zeolite in the co-composting of chicken manure and straw significantly reduced the emissions of ammonia and N2O, and increased the nitrate content. Zeolite also promoted the abundance of nitrification genes and inhibited the expression of denitrification genes.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Rohit Dey, Franziska Ortiz Tena, Song Wang, Josef Martin Messmann, Christian Steinweg, Claudia Thomsen, Clemens Posten, Stefan Leu, Matthias S. Ullrich, Laurenz Thomsen
Summary: This study investigated the operation of a 1000L microalgae-based membrane photobioreactor system for continuous secondary wastewater treatment. The research focused on a green microalgae strain called Desmodesmus sp. The study aimed to understand key trends and optimization strategies by conducting experiments in both summer and winter seasons. The findings showed that maintaining low cell concentrations during periods of light inhibition was beneficial for nutrient uptake rates. Effective strategies for enhancing algae-based wastewater treatment included cell mass recycling and adjusting dilution rates based on light availability.
BIORESOURCE TECHNOLOGY
(2024)
