Journal
CANCER LETTERS
Volume 472, Issue -, Pages 40-49Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2019.12.013
Keywords
Legumain; Cellular senescence; Tumor-associated macrophage; M1 polarization
Categories
Funding
- National Natural Science Foundation of China [81872254]
- Tianjin National Natural Science Foundation of China [16JCYBJC26300]
- National Youth Foundation of China [81902900]
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Macrophages serve as the first line of communication between tumors and the rest of the immune system, and understanding the interplay between macrophage and tumor cells is essential for developing novel macrophage-based strategy against tumor. Here, we show that deletion of legumain in macrophages activates senescence of tumor cells. Macrophage derived IL-1 beta mediates the pro-senescent effect of Lgmn(-/-) macrophages since blockage of IL-1 beta reverses the senescence phenotype in both a coculture model of macrophage and tumor cells and an orthotopic mouse model of breast cancer. Sustained activation of JAK1/STAT1 signaling and increased iNOS were found in the tumor cell-cocultured Lgmn(-)(/-) macrophages, which were necessary for IL-1 beta expression and secretion. Applying a specific STAT1 agonist mimics the inductive effect of legumain deletion on IL-10 expression in macrophages, and the effect can be blocked via inhibition of iNOS. Legumain and integrin alpha v beta 3 interact to prevent STAT1 signaling in macrophages, and blockage of integrin alpha v beta 3 stimulates STAT1 activation. Therapeutically, transplantation of bone marrow from Lgmn(-)(/-) mice suppresses the malignant growth of tumor by upregulating tumor cell senescence. Therefore, our finding highlights legumain in macrophages as a potential therapeutic target for tumors.
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