Article
Hematology
Sara Fananas-Baquero, Israel Orman, Federico Becerra Aparicio, Silvia Bermudez de Miguel, Jordi Garcia Merino, Rosa Yanez, Yolanda Fernandez Sainz, Rebeca Sanchez, Mercedes Dessy-Rodriguez, Omaira Alberquilla, David Alfaro, Agustin Zapata, Juan A. Bueren, Jose Carlos Segovia, Oscar Quintana-Bustamante
Summary: This study found that natural estrogens E2 and E4 can expand human hematopoietic stem and progenitor cells, with E4 being the most tolerated estrogen that promotes cycling of human hematopoietic progenitors. Additionally, E2 and E4 can double human hematopoietic engraftment in immunodeficient mice without altering other HSPC properties, potentially mediated through the regulation of mesenchymal stromal cells in the bone marrow niche.
Review
Cell & Tissue Engineering
Murilo Montenegro Garrigos, Fernando Anselmo de Oliveira, Mariana Penteado Nucci, Leopoldo Penteado Nucci, Arielly da Hora Alves, Olivia Furiama Metropolo Dias, Lionel Fernel Gamarra
Summary: This study aimed to analyze the characteristics and interactions of HSC and MSC through cotransplantation in murine models.
WORLD JOURNAL OF STEM CELLS
(2022)
Article
Medicine, General & Internal
Suzanne Bertera, Michael F. Knoll, Carmela Knoll, Hidetaka Hara, Erin A. Kimbrel, Nickolas A. Kouris, Robert Lanza, Brett E. Philips, Yesica Garciafigueroa, Nick Giannoukakis, David K. C. Cooper, Massimo Trucco, Rita Bottino
Summary: Islet transplantation is an important treatment for patients with type 1 diabetes, but success rates are not always consistent. Studies show that mesenchymal stem cells play a crucial role in islet transplantation, but a well-characterized and uniform cell product is needed for successful clinical translation.
FRONTIERS IN MEDICINE
(2021)
Article
Engineering, Biomedical
Roma Borkar, Xiaoyan Wang, Dejin Zheng, Zhengqiang Miao, Zhenwu Zhang, Enqin Li, Yaojiong Wu, Ren-He Xu
Summary: Research has shown that differentiation of embryonic stem cells into mesenchymal stem cells and transplantation with fat tissue into nude mice can enhance fat engraftment through tissue reconstitution, angiogenesis, increased adipocyte survival, and reduced fibrosis. This effect is mediated by stimulated CCL2 signaling and mobilized macrophages in promoting the engraftment of fat tissue.
Article
Cell & Tissue Engineering
Silvia Preciado, Sandra Muntion, Fermin Sanchez-Guijo
Summary: This review summarizes the potential of mesenchymal stromal cells to improve hematopoietic function through the secretion of extracellular vesicles after allogenic hematopoietic stem cell transplantation. It also discusses the advantages of EVs over MSCs, as well as the challenges and uncertainties that need to be addressed for their clinical application.
Article
Biochemistry & Molecular Biology
Yung-Tsung Kao, Chih-Ching Yen, Hueng-Chuen Fan, Jen-Kun Chen, Ming-Shan Chen, Ying-Wei Lan, Shang-Hsun Yang, Chuan-Mu Chen
Summary: This study investigated the use of human amniotic fluid mesenchymal stromal cells (hAFMSCs) through in utero transplantation (IUT) as a potential treatment for hemophilia A. The results showed that the transplanted human cells fused with the recipient liver and produced human FVIII protein. This approach significantly improved the coagulation issues in FVIII knockout mice with hemophilia A.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell & Tissue Engineering
Shaoxiong Yu, Saihua Yu, Haiyan Liu, Naishun Liao, Xiaolong Liu
Summary: This review summarizes the current status and challenges of mesenchymal stem cell (MSC) transplantation for end-stage liver disease (ESLD). It highlights the importance of improving cell engraftment efficiency in enhancing MSC therapy for liver diseases.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell & Tissue Engineering
Renata Gilfanova, Kirsten M. Auclair, Alvin Hui, Philip J. Norris, Marcus O. Muench
Summary: The study investigated the cryopreservation of human bone marrow using reduced concentrations of DMSO and found that 5% DMSO was as effective as higher concentrations in preserving hematopoietic stem cells. Different methods of analyzing stem cell engraftment in mice showed consistent results in supporting the cryopreservation of HSCs with reduced concentrations of DMSO.
Article
Cell & Tissue Engineering
Silvia Preciado, Ma Salome Sirerol-Piquer, Sandra Muntion, Lika Osugui, Gerardo J. Marti-Chillon, Almudena Navarro-Bailon, Pilar Sepulveda, Fermin Sanchez-Guijo
Summary: Co-transplantation of human CD34(+) cells with HIF-MSC enhances cell engraftment in vivo, possibly by increasing clonogenic capacity of hematopoietic cells and inducing the expression of adhesion molecules involved in graft survival in the hematopoietic niche.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell & Tissue Engineering
Dongxia Ge, Michael J. O'Brien, Felix H. Savoie, Jeffrey M. Gimble, Xiying Wu, Margaret H. Gilbert, Gabrielle L. Clark-Patterson, Jason D. Schuster, Kristin S. Miller, Alun Wang, Leann Myers, Zongbing You
Summary: Researchers successfully engineered cartilage tissues for repairing localized cartilage lesions by expressing doublecortin (DCX) in human adipose-derived stromal/stem cells (hASCs). The cartilage tissues with DCX expression significantly enhanced cartilage repair outcomes in rabbits and monkeys, as assessed macroscopically and histologically.
NPJ REGENERATIVE MEDICINE
(2021)
Article
Cell & Tissue Engineering
Huangfan Xie, Zhongjie Sun, Xiong Xiao, Defang Liu, Hailong Qi, Guoxiong Tian, Miao Chen, Ligong Chen, Xuncheng Su
Summary: Transient inhibition of the JNK pathway can increase the frequency of HSCs in cord blood and promote their quiescence, leading to the enhancement of HSC number and engraftment potential. These findings have the potential to improve HSC collection and transplantation.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Article
Cell Biology
Maria Serena Piccinno, Tiziana Petrachi, Marco Pignatti, Alba Murgia, Giulia Grisendi, Olivia Candini, Elisa Resca, Valentina Bergamini, Francesco Ganzerli, Alberto Portone, Ilenia Mastrolia, Chiara Chiavelli, Ilaria Castelli, Daniela Bernabei, Mara Tagliazucchi, Elisa Bonetti, Francesca Lolli, Giorgio De Santis, Massimo Dominici, Elena Veronesi
Summary: This study investigates the use of adipose-derived mesenchymal stromal/stem cells (AD-MSCs) in autologous fat transfer (AFT) for tissue reconstruction. The results show that AD-MSCs can improve graft persistence and reduce tissue resorption after AFT. The implanted AD-MSCs can persist and differentiate into mature adipocytes, leading to better outcomes in terms of graft weight and reduced cyst formation. This suggests a long-term protective and anti-inflammatory/proangiogenic role of AD-MSCs in autologous AFT tissue resorption.
Article
Biochemistry & Molecular Biology
Sanshiro Kanazawa, Hiroyuki Okada, Dan Riu, Yo Mabuchi, Chihiro Akazawa, Junichi Iwata, Kazuto Hoshi, Atsuhiko Hikita
Summary: This study demonstrates the important role of c-Mpl signal in the proliferation and differentiation ability of mesenchymal stem cells (MSCs), which are maintained through co-culture with hematopoietic stem cells (HSCs). Furthermore, the study suggests the existence of a hematopoietic-mesenchymal signal and highlights the significance of HSC state in the stability of MSC properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Dong-Geon Lee, Yu-Jin Lee, Song-Hee Park, Hye-Ree Park, Hoon Kang, Jung-Eun Kim
Summary: No effective therapeutic strategies have been developed against food allergies. Immunomodulation using human hematopoietic mesenchymal stem cells (hHMSCs) could prevent the development of food allergies. Treatment with hHMSCs or hHMSC culture medium significantly suppressed allergic responses, reduced diarrhea and allergy-related indicators. This treatment decreased Th2 cytokines levels and increased the expression of immune regulatory factors.
Article
Cell & Tissue Engineering
Jiaojiao Cheng, Xiangyan Ruan, Yanglu Li, Juan Du, Fengyu Jin, Muqing Gu, Qi Zhou, Xin Xu, Yu Yang, Husheng Wang, Alfred Otto Mueck
Summary: Co-transplantation of hypoxia-preconditioned human umbilical cord mesenchymal stem cells (HucMSCs) with ovarian tissue can improve neovascularization and subsequently increase follicle survival in human ovarian tissue. This has significant implications for ovarian tissue cryopreservation and transplantation.
STEM CELL RESEARCH & THERAPY
(2022)
Letter
Hematology
Wenhui Zhang, Xuewen Song, Xueqing Dou, Man-Chiu Poon, Wei Liu, Lingling Chen, Lei Zhang, Feng Xue, Renchi Yang
Article
Multidisciplinary Sciences
Lu Zhang, Haiyan Cui, Mingyu Zhu, Kuo Zhao
Summary: CRISPR-Cas9 system is a gene editing technology that can perform various genetic operations on specific sites of the genome, showing broad application prospects. By analyzing the dynamic changes of CRISPR-Cas9 system in iPSCs, new genes can be explored to improve knockin efficiency; reducing cell death and interfering with DNA damage repair signal may be important ways to improve efficiency when treating iPSCs.
IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A-SCIENCE
(2022)
Article
Hematology
Guangshuai Teng, Yuan Zhou, Yuhui Zhang, Naibo Hu, Tong Liu, Yingdi Han, Chao Gao, Lei Zhang, Jie Bai
Summary: In patients with post-polycythemia vera myelofibrosis, the incidence of thrombosis is higher. Palpable splenomegaly, age above 60 years, and history of thrombosis are independent risk factors for thrombosis.
THROMBOSIS RESEARCH
(2022)
Article
Immunology
Mingyu Zhu, Lu Zhang, Haiyan Cui, Qiang Zhao, Hao Wang, Baochao Zhai, Richeng Jiang, Zhansheng Jiang
Summary: Through comprehensive analysis of co-mutated genes in lung adenocarcinoma samples, the co-mutation of FAT3 and LRP1B is found to be associated with age and ethnicity in lung adenocarcinoma patients, and it can impact tumor burden, cytotoxicity, tumor immunogenicity, and lymphocyte infiltration. Additionally, the subset of LUAD with co-mutation of FAT3 and LRP1B exhibits significantly prolonged immunotherapy progression free survival.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Mingyu Zhu, Haiyan Cui, Lu Zhang, Kuo Zhao, Xiaochen Jia, Hao Jin
Summary: POLE and POLD1 mutations are found in 7.99% of STAD patients, associated with older age of onset and more frequent occurrence in the antrum anatomic subdivisions. STAD patients with POLE/POLD1 mutations have longer PFS and OS, as well as adaptive immune resistance TME.
TRANSLATIONAL CANCER RESEARCH
(2022)
Article
Oncology
Shuhang Wang, Pei Yuan, Beibei Mao, Ning Li, Jianming Ying, Xiuli Tao, Wei Tang, Lei Zhang, Xiao Geng, Fan Zhang, Qi Xue, Lijia Wu, Henghui Zhang, Shugeng Gao, Jie He
Summary: This study assessed the association of PD-L1 expression, genomic factors, and the response to neoadjuvant immunotherapy in lung cancer. The changes in the tumor immune microenvironment during treatment were also investigated.
NPJ PRECISION ONCOLOGY
(2022)
Article
Oncology
Ning Lou, Cuiling Zheng, Yanrong Wang, Caixia Liang, Qiaoyun Tan, Rongrong Luo, Lei Zhang, Tongji Xie, Yuankai Shi, Xiaohong Han
Summary: This study provides a comprehensive profiling of autoantibodies in Chinese prostate cancer patients and identifies novel diagnostic autoantibody biomarkers, especially for the identification of gray-zone PSA patients.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2023)
Article
Hematology
Cuiyun Qu, Wei Liu, Lingling Chen, Lei Zhang, Feng Xue, Renchi Yang
Summary: This study investigated the clinical phenotype and genotype of patients with congenital factor VII (FVII) deficiency in China and showed their current management status. The majority of patients had FVII activity <= 10%, which was associated with various bleeding symptoms. Prothrombin complex concentrates were the most frequently used on-demand replacement therapy. Prophylaxis before delivery decreased the risk of postpartum bleeding in women of reproductive age.
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Article
Hematology
Luyang Zhang, Xiaoli Chen, Tianyuan Hu, Zefeng Xu, Wenyu Yang, Rongfeng Fu, Lei Zhang, Xiaofan Zhu
Summary: Little is known about the pathophysiological mechanism of essential thrombocythemia (ET) in children due to its rarity. A retrospective analysis of 40 Chinese children with ET from 2015-2021 revealed that more than half of them were asymptomatic at diagnosis. Microvascular symptoms were observed in nearly half of the children, while only two cases experienced vascular events. Driver gene mutations were found in a low proportion of children, and the most prevalent non-driver gene mutations were less common compared to adults with ET.
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Article
Hematology
Wenjing Gu, Jingyuan Tong, Rongfeng Fu, Ting Sun, Mankai Ju, Yanhong Zhao, Di Wang, Jie Gao, Jinhua Liu, Yuchen Gao, Huiyuan Li, Wentian Wang, Ying Chi, Renchi Yang, Lu Chen, Lihong Shi, Lei Zhang
Summary: JAK2V617F is the most frequent mutation in BCR-ABL-negative myeloproliferative neoplasms (MPNs). Two concurrent mutations in SH2B3 and SF3B1 were identified, which may affect the phenotype of MPNs. Transcriptomic analysis revealed elevated inflammatory signaling pathways in ET HSCs and MEPs, and upregulation of Wnt/beta-catenin signaling during ET hematopoietic differentiation. Inhibition of Wnt/beta-catenin signaling decreased platelet counts in JAK2V617F(+) MPN mice. Overall, these findings provide insights into phenotypic differentiation of JAK2V617F(+) PV and ET and suggest Wnt/beta-catenin signaling as a potential therapeutic target for MPNs.
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Article
Hematology
Wenhui Zhang, Kuixing Li, Man-Chiu Poon, Xinsheng Zhang, Jingsheng Wu, Xielan Zhao, Min Zhou, Xuefeng Wang, Mei Zhang, Weiqun Xu, Linhua Yang, Meijuan Huang, Chenghao Jin, Xiaomin Wang, Donglei Zhang, Lingling Chen, Wei Liu, Lei Zhang, Feng Xue, Renchi Yang
Summary: This study is the first and largest in China to describe women and girls with haemophilia. It found deficiencies in the identification, diagnosis, and management of these patients. Improving health insurance policies, establishing haemophilia centres, and creating multidisciplinary teams for bleeding and perinatal or perioperative management will help reduce morbidity and mortality.
Article
Respiratory System
Xiaoxuan Zheng, Changhao Zhong, Fangfang Xie, Shiyue Li, Guiqi Wang, Lei Zhang, Jiayuan Sun
Summary: This study compared the diagnostic utility of virtual bronchoscopic navigation (VBN) and radial endobronchial ultrasound with a guide sheath (rEBUS-GS) with or without fluoroscopy for transbronchial sampling of peripheral pulmonary lesions (PPLs). The results showed that the diagnostic yield was similar in both groups, and the group without fluoroscopy had shorter operation time and no severe complications.
Article
Oncology
Yuhui Zhang, Yingdi Han, Guangshuai Teng, Chenxiao Du, Shan Gao, Weiping Yuan, Lei Zhang, Jie Bai
Summary: This study retrospectively analyzed the clinical characteristics and survival of 1060 Chinese patients with myeloproliferative neoplasms (MPNs) to explore the risk factors for secondary cancer in MPN patients. The results showed that the survival of MPN patients with secondary cancer was significantly worse, and age >= 65 years and MF-1 were identified as risk factors for developing secondary cancer.
Article
Hematology
Zimin Sun, Yaming Xi, Wei Liu, Linhua Yang, Xuefeng Wang, Chenghao Jin, Haifei Jia, Lei Zhang
Summary: This study evaluated the efficacy and safety of TQG202, a human-derived recombinant factor VIII, in the on-demand treatment of bleeding episodes in moderate/severe haemophilia A patients. The results showed that TQG202 effectively controlled bleeding symptoms with a low incidence of adverse events and inhibitor development.
Article
Biology
Wanqiu Chen, Chenguang Wang, Zhi-Xue Yang, Feng Zhang, Wei Wen, Christoph Schaniel, Xianqiang Mi, Matthew Bock, Xiao-Bing Zhang, Hongyu Qiu, Charles Wang
Summary: Researchers have developed a method to generate induced mesenchymal stromal cells (iMSCs) by directly reprogramming human peripheral blood mononuclear cells (PBMCs) using five factors. Omission of OCT4, one of the factors, significantly impairs the functionality of iMSCs and downregulates MSC-specific and mesoderm-regulating genes. The transient expression of OCT4 increases chromatin accessibility and promotes demethylation.
COMMUNICATIONS BIOLOGY
(2023)
