Article
Microbiology
Madalina Iulia Siteavu, Roxana Ionela Drugea, Elena Pitoiu, Emilia Ciobotaru-Pirvu
Summary: Antimicrobial resistance is an important health issue in human and veterinary medicine. This study monitored the antimicrobial resistance of three key bacteria in porcine respiratory disease. The results showed that these bacteria maintained high susceptibility to commonly used antimicrobial agents, but had high resistance rates to certain drugs.
Article
Immunology
Li Yi, Manyu Jin, Mengxia Gao, Haikun Wang, Qingying Fan, Daniel Grenier, Liyun Sun, Shaohui Wang, Yang Wang
Summary: Respiratory infections greatly impact the swine industry globally, with co-infections of Streptococcus suis and Actinobacillus pleuropneumoniae often forming biofilms. However, the methods for detecting these bacteria in co-infections and biofilms are immature. This study successfully established a TaqMan real-time PCR method for simultaneous detection of S. suis and A. pleuropneumoniae, which may aid in disease detection, prevention, and control.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Rea Jarosova, Petra Ondrackova, Lenka Leva, Katerina Nedbalcova, Monika Vicenova, Josef Masek, Jiri Volf, Jan Gebauer, Tomas Do, Roman Guran, Zbysek Sladek, Javier Dominguez, Martin Faldyna
Summary: CD163+ monocytes contribute to the production of inflammatory cytokines during APP-induced lung inflammation, especially IL-1 beta, at the site of inflammation. PB and BM CD163+ monocytes do not appear to play a significant role in elevated cytokine levels in plasma.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Microbiology
Julia Tetens, Sabrina Sprotte, Georg Thimm, Natalia Wagner, Erik Brinks, Horst Neve, Christina Susanne Hoelzel, Charles M. A. P. Franz
Summary: Exudative epidermitis (EE), commonly known as greasy pig disease, is a frequent skin disease affecting piglets, with potential zoonotic infections in humans. It is primarily caused by virulent strains of Staphylococcus (S.) hyicus. The discovery of a new virulent phage offers a new approach for the treatment of greasy pig disease.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Rea Jarosova, Juliette Ben Arous, Katerina Nechvatalova, Katerina Nedbalcova, Karolina Hlavova, Hana Stepanova, Lenka Leva, Zrinka Oreskovic, Jan Matiasovic, Nicolas Versille, Zbysek Sladek, Martin Faldyna
Summary: The route of vaccine delivery significantly affects its immunogenicity, efficacy, and safety. In this study, different routes of delivery were compared in piglets, and it was found that intramuscular administration showed the highest efficacy while dermal administration was slightly less immunogenic. Interestingly, dermal administration provided superior protection effectiveness compared to other routes.
VETERINARY MICROBIOLOGY
(2023)
Article
Infectious Diseases
Andrea Micke Moreno, Luisa Zanolli Moreno, Andre Pegoraro Poor, Carlos Emilio Cabrera Matajira, Marina Moreno, Vasco Tulio de Moura Gomes, Givago Faria Ribeiro da Silva, Karine Ludwig Takeuti, David Emilio Barcellos
Summary: This study evaluated the changes in antimicrobial resistance profiles of S. hyicus strains over time and found significant differences between different periods. The results also suggest that S. hyicus could be a useful indicator in antimicrobial resistance monitoring programs in swine production.
Article
Biotechnology & Applied Microbiology
He Liu, Na Yang, Da Teng, Ruoyu Mao, Ya Hao, Xuanxuan Ma, Xiumin Wang, Jianhua Wang
Summary: By conjugating antimicrobial peptides with fatty acids, this study found that the derived peptides Lin-Lf4NH2 and Lin-Lf5NH2 demonstrated improved antibacterial activity and superior topical therapeutic efficacy in a mouse model infected with Staphylococcus hyicus.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Immunology
Natalie Kralova, Hana Stepanova, Jan Gebauer, Adam Norek, Katarina Matiaskova, Monika Zouharova, Katerina Nedbalcova, Vladimir Babak, Rea Jarosova, Peter Makovicky, Ivana Kucharovicova, Bronislav Simek, Hana Plodkova, Tomas Pecka, Jan Matiasovic
Summary: This study tested an experimental vaccine against Streptococcus suis serotype 2 based on capsular polysaccharide conjugated to chicken ovalbumin (OVA) and compared its immunogenicity and protectivity with a vaccine based on CRM197 conjugate. The results showed that the ovalbumin-based vaccine successfully induced immune response in pigs and the antibody response was even higher than after immunization with capsular polysaccharide-CRM197 conjugate.
Article
Veterinary Sciences
Katy Vaillancourt, Michel Frenette, Marcelo Gottschalk, Daniel Grenier
Summary: This study investigated the H2O2-dependent antagonistic activity of Streptococcus pluranimalium against Actinobacillus pleuropneumoniae. Results showed that H2O2 was effective in killing A. pleuropneumoniae and the bactericidal property of S. pluranimalium's culture supernatant was abolished after treatment with catalase. Additionally, H2O2 exhibited antibacterial synergistic interactions with conventional antibiotics, particularly ceftiofur, suggesting its potential as a protective mechanism against H2O2-sensitive pathogens in the upper respiratory tract.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Microbiology
Joao Pires, Laura Huber, Rachel A. Hickman, Simon Dellicour, Kamonwan Lunha, Thongpan Leangapichart, Jatesada Jiwakanon, Ulf Magnusson, Marianne Sunde, Josef D. Jarhult, Thomas P. Van Boeckel
Summary: Thailand is experiencing differences in antimicrobial use and the composition of antimicrobial resistance genes (ARGs) between small and medium farms. Pig isolates from small farms are more associated with genes conferring resistance to colistin and fluoroquinolones, while pig isolates from medium farms are associated with genes conferring resistance to drugs commonly used on medium farms. ARGs conferring resistance to critically important antimicrobials are more frequently co-carried on plasmids from small farms compared to plasmids from medium farms. Actions to curb the spread of these ARGs are urgently needed.
Article
Medicine, General & Internal
Javier Diez de los Rios, Esteban Reynaga, Merce Garcia-Gonzalez, Jordi Camara, Carmen Ardanuy, Jordi Cuquet, Maria D. Quesada, Marian Navarro, Anna Vilamala, Noemi Parraga-Nino, Sara Quero, Alba Romero, Rosa M. Benitez, Jacint Altimiras, Maria Luisa Pedro-Botet
Summary: The study focused on S. suis infections in Catalonia over a 9-year period, revealing a small number of cases predominantly affecting middle-aged males, with meningitis being the most common manifestation. More than half of the cases occurred in the last two years and were mainly seen in pig farm workers. While none of the patients died within 30 days, some did experience long-term complications such as hearing loss. Further surveillance is recommended to better understand the prevalence of S. suis infections in the region.
FRONTIERS IN MEDICINE
(2021)
Article
Agriculture, Dairy & Animal Science
Frederic A. C. J. Vangroenweghe, Mieke Boone
Summary: This study demonstrates the efficacy of an oral live non-pathogenic E. coli vaccine for active immunization of piglets against post-weaning diarrhea (PWD) under field conditions. The vaccine-treated groups showed significant improvements in piglet performance, reduced mortality weight, and decreased antimicrobial use. Additionally, the vaccine reduced secondary infections caused by Streptococcus suis in the second phase of nursery. Vaccination against PWD can be considered a valuable alternative for improving piglet performance while meeting the new EU requirements for the prudent use of antimicrobials in intensive pig production.
Article
Microbiology
Nichari Bamphensin, Peechanika Chopjitt, Rujirat Hatrongjit, Parichart Boueroy, Nahuel Fittipaldi, Marcelo Gottschalk, Anusak Kerdsin
Summary: S. suis isolates from human infections in Thailand showed susceptibility to cefepime and ceftriaxone, but high resistance to tetracycline, clindamycin, erythromycin, and azithromycin. Macrolide- and tetracycline-resistant isolates had predominant resistance genes tet(O) and ermB. Penicillin-intermediate resistance isolates mainly belonged to serotype 2-ST233. Analysis of PBP protein sequences revealed multiple amino acid substitutions in non-penicillin-susceptible isolates.
Article
Microbiology
Manao Ozawa, Yukari Furuya, Ryoko Akama, Saki Harada, Mari Matsuda, Hitoshi Abo, Takahiro Shirakawa, Michiko Kawanishi, Eiji Yoshida, Minako Furuno, Hisae Fukuhara, Kazufumi Kasuya, Yoko Shimazaki
Summary: Methicillin-resistant Staphylococcus aureus (MRSA) is a common infection in hospitalized patients and can also be found in pigs. This study aimed to determine the prevalence of MRSA in pigs and farm environments in Japan. Whole-genome sequencing was conducted and the molecular epidemiological relationship between MRSA isolates was analyzed. The results showed varying prevalence rates of MRSA among different sources and the presence of resistance genes in most MRSA isolates. Molecular epidemiological analysis suggested a relationship between isolates from slaughter pigs and imported breeding pigs and the presence of MRSA isolates of domestic origin. More data are needed for further understanding of the origin of these MRSA variants in the pig industry in Japan.
VETERINARY MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Shanshan Zhang, Hongbing Xie, Mingcheng Liu, Airong Zheng, Haixing Yan, Mingyuan Duan, Xiaobing Wei, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: A dual recombinant enzyme polymerase amplification (RPA)-lateral flow dipstick (LFD) detection method was established for the rapid identification of S. suis and A. pleuropneumoniae. The method exhibited good specificity and high sensitivity, making it suitable for preliminary screening of clinical diseases.
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
(2022)
Article
Microbiology
Julie E. Hansen, Marc Stegger, Karl Pedersen, Raphael N. Sieber, Jesper Larsen, Gitte Larsen, Berit Lilje, Mariann Chriel, Paal S. Andersen, Anders R. Larsen
VETERINARY MICROBIOLOGY
(2020)
Article
Food Science & Technology
Johanne Ellis-Iversen, Mette R. Gantzhorn, Birgitte Borck Hog, Alessandro Foddai, Maarten Nauta
Review
Veterinary Sciences
Pim Sanders, Wannes Vanderhaeghen, Mette Fertner, Klemens Fuchs, Walter Obritzhauser, Agnes Agunos, Carolee Carson, Birgitte Borck Hog, Vibe Dalhoff Andersen, Claire Chauvin, Anne Hemonic, Annemarie Kaesbohrer, Roswitha Merle, Giovanni L. Alborali, Federico Scali, Katharina D. C. Staerk, Cedric Muentener, Ingeborg van Geijlswijk, Fraser Broadfoot, Lucie Pokludova, Clair L. Firth, Luis P. Carmo, Edgar Garcia Manzanilla, Laura Jensen, Marie Sjolund, Jorge Pinto Ferreira, Stacey Brown, Dick Heederik, Jeroen Dewulf
FRONTIERS IN VETERINARY SCIENCE
(2020)
Article
Infectious Diseases
Rodolphe Mader, Peter Damborg, Jean-Philippe Amat, Bjorn Bengtsson, Clemence Bourely, Els M. Broens, Luca Busani, Paloma Crespo-Robledo, Maria-Eleni Filippitzi, William Fitzgerald, Heike Kaspar, Cristina Munoz Madero, Madelaine Norstrom, Suvi Nykasenoja, Karl Pedersen, Lucie Pokludova, Anne Margrete Urdahl, Alkiviadis Vatopoulos, Christos Zafeiridis, Jean-Yves Madec
Summary: Establishing the European Antimicrobial Resistance Surveillance network in Veterinary medicine (EARS-Vet) is proposed to fill the gap in current AMR surveillance efforts, provide useful information for policymakers and veterinary practitioners, and support antimicrobial stewardship in animal health. Strong political commitment at national and EU/EEA levels is required for the success of EARS-Vet.
Article
Infectious Diseases
Laerke Boye Astrup, Julie Elvekjaer Hansen, Karl Pedersen
Summary: Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is prevalent in European pig production, with increasing infections in humans. The bacterium was found in liquid pig manure and fertilized soils, but liquid manure does not appear to be a significant risk factor for human exposure to LA-MRSA.
Article
Microbiology
Diana Gutierrez, Lorena Rodriguez-Rubio, Patricia Ruas-Madiedo, Lucia Fernandez, Ana Belen Campelo, Yves Briers, Martin Weiss Nielsen, Karl Pedersen, Rob Lavigne, Pilar Garcia, Ana Rodriguez
Summary: A series of engineered proteins based on endolysins were designed and screened, with LysRODI Delta Ami identified as having high lytic activity, stability, and antimicrobial properties to effectively prevent and treat Staphylococcus aureus skin infections.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Anna Duse, Karin Persson-Waller, Karl Pedersen
Summary: The study revealed the most important bacterial pathogens and their resistance patterns in Sweden regarding mastitis, with low antibiotic resistance among both Gram-positive and Gram-negative agents, indicating good potential for antibiotic treatment.
Article
Infectious Diseases
Rodolphe Mader, Clemence Bourely, Jean-Philippe Amat, Els M. Broens, Luca Busani, Benedicte Callens, Paloma Crespo-Robledo, Peter Damborg, Maria-Eleni Filippitzi, William Fitzgerald, Thomas Gronthal, Marisa Haenni, Annet Heuvelink, Jobke van Hout, Heike Kaspar, Cristina Munoz Madero, Madelaine Norstrom, Karl Pedersen, Lucie Pokludova, Fabiana Dal Pozzo, Rosemarie Slowey, Anne Margrete Urdahl, Alkiviadis Vatopoulos, Christos Zafeiridis, Jean-Yves Madec
Summary: The European Antimicrobial Resistance Surveillance network in Veterinary medicine (EARS-Vet) aims to strengthen the surveillance of antimicrobial resistance (AMR) in the animal sector in Europe. It plans to monitor AMR in six animal species and 11 bacterial species, aiming to fill important monitoring gaps and complement existing European AMR monitoring systems.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Veterinary Sciences
Nanett Kvist Nikolaisen, Mikkel Lindegaard, Ulrike Lyhs, Mikael Lenz Strube, Mette Sif Hansen, Tina Struve, Mariann Chriel, Lars Bogo Jensen, Karl Pedersen
Summary: This study reports the first finding of S. phocae infection in farmed mink, with close relations between isolates from mink and seals. The infected animals showed symptoms such as pulmonary infections and septicemia, indicating S. phocae is associated with the pathology of the infection. Further research is required to determine if S. phocae is establishing as a commensal organism in farmed mink.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Infectious Diseases
Laerke Boye Astrup, Karl Pedersen, Michael Farre
Summary: This study compares the accuracy of diagnoses on bovine mastitis made by veterinary clinics using conventional methods with diagnoses made by a reference laboratory using MALDI-TOF MS as a diagnostic tool. The findings show that a significant number of diagnoses made by veterinary clinics are incorrect, resulting in the potential misuse of antibiotics.
Review
Microbiology
Rodolphe Mader, Cristina Munoz Madero, Birgit Aasmae, Clemence Bourely, Els M. Broens, Luca Busani, Benedicte Callens, Lucie Collineau, Paloma Crespo-Robledo, Peter Damborg, Maria-Eleni Filippitzi, William Fitzgerald, Annet Heuvelink, Jobke van Hout, Heike Kaspar, Madelaine Norstrom, Karl Pedersen, Tarja Pohjanvirta, Lucie Pokludova, Fabiana Dal Pozzo, Rosemarie Slowey, Cristiana Teixeira Justo, Anne Margrete Urdahl, Alkiviadis Vatopoulos, Christos Zafeiridis, Jean-Yves Madec, Jean-Philippe Amat
Summary: The monitoring of antimicrobial resistance in animal bacterial pathogens is not coordinated at a European level. Experts have recommended the establishment of the European Antimicrobial Resistance Surveillance network in Veterinary medicine (EARS-Vet). This study identified national monitoring systems in 27 EU-JAMRAI-affiliated countries, described their structures and operations, and analyzed their strengths, weaknesses, opportunities, and threats. The findings highlight important gaps in AMR data generation in Europe and provide useful information for countries planning to build or improve their systems.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Environmental Sciences
Alessandro Foddai, Nao Takeuchi-Storm, Birgitte Borck Hog, Jette Sejer Kjeldgaard, Jens Kirk Andersen, Johanne Ellis-Iversen
Summary: The study investigates the cross-contamination of Campylobacter in Danish broiler flocks at slaughterhouses, and finds that the occurrence of cross-contamination can be affected by surveillance periods and slaughterhouses, contributing significantly to the prevalence of carcass positive flocks.
MICROBIAL RISK ANALYSIS
(2022)
Article
Agriculture, Dairy & Animal Science
E. Hurri, A. Ohlson, A. Lundberg, A. Aspan, K. Pedersen, M. Traven
Summary: This study investigated the prevalence of Mycoplasma bovis in Swedish dairy herds and the herd characteristics associated with the presence of antibodies in bulk tank milk (BTM). The results showed regional differences in the apparent prevalence of M. bovis infection based on antibodies, and larger herds had higher odds of detectable antibodies in BTM. There was also a correlation between antibodies in BTM and higher calf mortality rates. These findings are important for preventing the spread of infection and introducing cattle from high-prevalence areas into herds.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Infectious Diseases
Nanett Kvist Nikolaisen, Mette Fertner, Desiree Corvera Klove Lassen, Chaza Nazih Chehabi, Amir Atabak Ronaghinia, Mariann Chriel, Vibeke Frokjor Jensen, Lars Bogo Jensen, Karl Pedersen, Tina Struve
Summary: There is a link between antibiotic consumption and resistance in mink breeding. Short-term and long-term use of specific antibiotics is associated with the occurrence of resistant bacteria. Antibiotic-resistant bacteria can persist in the environment even on farms with no antibiotic consumption.
Article
Multidisciplinary Sciences
Gergana Mateva, Karl Pedersen, Gitte Sorensen, Mia Torpdahl, Hristo Daskalov, Charlotta Lofstrom, Irina Alexandar
Summary: Salmonellosis, caused by food infected with Salmonella enterica serovar Enteritidis, is a common global pathogen. This study analyzed 49 S. Enteritidis isolates from Bulgaria and found diverse MLVA and resistance profiles, with partial matches to isolates from Belgium, Thailand, China, and USA.
COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES
(2021)