Journal
BIOTECHNOLOGY LETTERS
Volume 42, Issue 5, Pages 797-806Publisher
SPRINGER
DOI: 10.1007/s10529-020-02810-7
Keywords
Bacteriocins; Listeria monocytogenes; Bacillus sp; Enterococcus sp; Food protections; Lactic acid bacteria
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Funding
- Fundacao Araucaria/Governo do Parana - Brazil, PROPPG/UTFPR
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-Brasil (CAPES) [001]
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Objective To determine bacteriocin producers and the prevalence of structural enterocin genes and to detect the spectrum of activity against foodborne pathogens, from isolates of Enterococcus faecium and Enterococcus faecalis that were isolated from food and the environment. Results The entA, entB, entP, ent1071 and entX genes, which encode enterocins were the most frequently observed. Enterocins were thermostable, proteinaceous, and resistant to catalase. None of the isolates produced hemolysin, and inhibition resulting from bacteriophage lysis was excluded. The bactericidal effect of enterocins against L. innocua 12612 was determined by optical density and colony forming units. For the activity spectrum, elimination of mainly Listeria strains, Bacillus sp. and clinical enterococci, was observed. Imaging with scanning electron microscopy after treatment with enterocin Efm22 showed irregular rod-shaped cells and loss of cellular integrity. Conclusions The isolates evaluated in this study are candidates for the production of enterocins that will be used as food biopreservatives, because they have high anti-listerial activity even after 24 h of experimentation, and used in the pharmaceutical area because they inhibit clinical microorganisms.
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