Article
Biochemistry & Molecular Biology
Anton G. Rogov, Tatiana N. Goleva, Khoren K. Epremyan, Igor I. Kireev, Renata A. Zvyagilskaya
Summary: Mitochondria are considered the primary source of ROS in cells, with ROS-induced ROS release triggered in cardiac myocytes under excessive oxidative stress. Using Dipodascus magnusii yeast cells, it was found that prooxidant-induced oxidative stress begins in mitochondria before spreading to the whole cell, with mitochondrial ROS leading to mitochondrial fragmentation.
Article
Plant Sciences
Maolong Hu, Hongkun Zhang, Lingna Kong, Juanjuan Ma, Ting Wang, Xinyu Lu, Yue Guo, Jiefu Zhang, Rongzhan Guan, Pu Chu
Summary: The application of herbicides is an effective strategy for weed control, and the development of herbicide-resistant crops can enhance weed management. However, the use of the herbicide tribenuron-methyl (TBM) is limited in rapeseed fields due to rapeseed's sensitivity to TBM. A study was conducted on the TBM-resistant rapeseed mutant M342 and its wild-type plants, revealing that M342 exhibited improved tolerance to TBM and had higher levels of proteins related to non-target-site resistance (NTSR) to herbicides compared to the wild-type plants. Differentially accumulated proteins (DAPs) between the two genotypes were enriched in glutathione metabolism and oxidoreduction coenzyme metabolic process, providing protection against oxidative stress caused by TBM. These findings offer insights into the mechanism of NTSR in plants and contribute to the development of herbicide-resistant crops.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Caiyue Liu, Tianbao Zhang, Xinsen Yang, Liu Wang, Yan Long, Agula Hasi, Xinwu Pei
Summary: In this study, a mutant with resistance to ALS-inhibiting herbicides was obtained from the elite cultivar Longya10 through EMS mutagenesis. The mutant showed significantly increased resistance to TBM and slight resistance to imazethapyr compared to Longya10. Biochemical analysis revealed a Pro197Ser mutation in the LuALS1 gene, which conferred high resistance to sulfonylurea herbicides in the mutant. Two CAPS markers were developed based on this mutation site for marker assistant selection in breeding. The mutant did not cause yield losses in natural field conditions, making it a valuable germplasm resource for breeding herbicide-resistant flax varieties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yi Cao, Yuning Lan, Hongjuan Huang, Shouhui Wei, Xiangju Li, Ying Sun, Ruolin Wang, Zhaofeng Huang
Summary: The green foxtail weed in China has developed a strong resistance to the herbicide nicosulfuron due to excessive use, with a 35.8-fold resistance observed in the R376 population. Resistance is caused by a mutation in the ALS gene and enhanced metabolism, involving ten candidate genes including ABC transporters, P450 enzymes, UGT, and GST.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Vanessa Vital Silva, Rafael Mendes, Andreia Suzukawa, Fernando Adegas, Francismar Marcelino-Guimaraes, Rubem Oliveira
Summary: The study confirmed cross-resistance of a weed population in Brazil to ALS inhibitors and identified a mutation in the ALS gene. This is the first study in Brazil to identify a target-site change as a survival mechanism in Erigeron sumatrensis for resistance to ALS-inhibiting herbicides.
Article
Plant Sciences
Ryan Widianto, Denny Kurniadie, Dedi Widayat, Uum Umiyati, Ceppy Nasahi, Santika Sari, Abdul Shukor Juraimi, Hisashi Kato-Noguchi
Summary: Monochoria vaginalis is an aquatic herbaceous plant that is often found as a noxious weed in paddy fields. This research evaluated the resistance levels of M. vaginalis to different groups of herbicides and investigated the mutations in the ALS gene of the resistant biotype. The study found that the resistant biotype had varying levels of resistance to different herbicides and had specific amino acid substitutions in the ALS protein. Further research is needed to understand the molecular basis of resistance mechanisms and to develop control strategies.
Article
Agronomy
Yi Cao, Xinxin Zhou, Zhaofeng Huang
Summary: The study revealed the molecular basis for resistance of Amaranthus retroflexus to ALS-inhibiting herbicides, with Trp-574-Leu and Gly-654-Tyr substitutions in ALS contributing to broad-spectrum resistance. The Gly-654-Tyr substitution, previously unreported, conferred high resistance to multiple classes of ALS-inhibiting herbicides in the R-Tyr654 population. Despite this resistance, effective control of the population was achieved with herbicides targeting other sites of action.
PEST MANAGEMENT SCIENCE
(2022)
Article
Biology
Rui Zhang, Sha Chen, Xiangbing Meng, Zhuangzhuang Chai, Delin Wang, Yuge Yuan, Kunling Chen, Linjian Jiang, Jiayang Li, Caixia Gao
Summary: Utilizing genome editing, various missense mutations were created in rice to confer tolerance to different herbicides, with a novel triple mutant showing high tolerance to all tested herbicides. Field trials suggested that these mutated varieties have potential practical applications in weed control.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Agronomy
Valeria E. Palmieri, Clarisa E. Alvarez, Hugo R. Permingeat, Valeria E. Perotti
Summary: This study compared various mutations of the Amaranthus palmeri ALS gene in vitro and found that certain mutations led to a decrease in catalytic efficiency and resistance to herbicides. The A122S mutation provided cross-resistance to different families of ALS inhibitors.
PEST MANAGEMENT SCIENCE
(2022)
Article
Plant Sciences
Candelario Palma-Bautista, Jose G. Vazquez-Garcia, Maria D. Osuna, Blanca Garcia-Garcia, Joel Torra, Joao Portugal, Rafael De Prado
Summary: A study in southern Spain found that Sinapis alba populations have developed resistance to the acetolactate synthase-inhibiting herbicide tribenuron-methyl. The resistant populations showed significantly higher resistance to tribenuron-methyl compared to the susceptible population. A mutation in the ALS gene and P450-mediated metabolism were identified as contributing factors to the resistance. Alternative chemical control methods were effective in controlling both the resistant and susceptible populations.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Yanfei Zhang, Jeremy D. Cortez, Sarah K. Hammer, Cesar Carrasco-Lopez, Sergio A. Garcia Echauri, Jessica B. Wiggins, Wei Wang, Jose L. Avalos
Summary: The authors report a genetically encoded biosensor for branched-chain amino acid (BCAA) metabolism based on the Leu3p transcriptional regulator in Saccharomyces cerevisiae. This biosensor is used to monitor yeast production of isobutanol and isopentanol. The biosensor configurations are effective in isolating high-producing strains and identifying enzymes with enhanced activity.
NATURE COMMUNICATIONS
(2022)
Article
Agronomy
Lei Chen, Gang Gu, Chengxu Wang, Zhufeng Chen, Wei Yan, Man Jin, Gang Xie, Junli Zhou, Xing Wang Deng, Xiaoyan Tang
Summary: Herbicide resistance is valuable for integrated weed management in agriculture, particularly in rice fields where weedy rice poses a challenge. This study obtained three mutant variants of the OsALS gene in rice through ethyl methyl sulfonate mutagenesis, with W-548 M mutant showing high levels of resistance to a broad spectrum of herbicides. The stable inheritance of herbicide resistance in rice lines makes W-548 M mutation a valuable resource for breeding herbicide resistant rice and weed management.
Article
Plant Sciences
Sarah Shah, Thierry Lonhienne, Cody-Ellen Murray, Yibi Chen, Katherine E. Dougan, Yu Shang Low, Craig M. Williams, Gerhard Schenk, Gimme H. Walter, Luke W. Guddat, Cheong Xin Chan
Summary: This study identified 12 common herbicide-target genes and their proteins from seven species of Weeds of National Significance in Australia using high-throughput genome sequencing. The study found high sequence conservation at the herbicide-target sites among the diverse weed species. Mutations observed in the proteins of fireweed and parthenium suggest resistance of these weeds to certain herbicides. These findings provide important insights for herbicide discovery and development.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Maria Barco-Antonanzas, Miriam Gil-Monreal, Mikel Eceiza, Mercedes Royuela, Ana Zabalza
Summary: This study characterized the resistance mechanisms and metabolism of a multiple resistant population of Amaranthus palmeri to glyphosate and pyrithiobac. The results showed that glyphosate resistance was due to EPSPS gene amplification, while resistance to pyrithiobac was related to point mutations in the ALS gene sequence. The study also found additional effects of EPSPS gene amplification and ALS mutations on the expression of genes in certain metabolic pathways. The physiological response of the resistant population to glyphosate and pyrithiobac was lower compared to the sensitive population.
Article
Plant Sciences
Mikel Vicente Eceiza, Maria Barco-Antonanzas, Miriam Gil-Monreal, Michiel Huybrechts, Ana Zabalza, Ann Cuypers, Mercedes Royuela
Summary: The aim of this study was to investigate the role of oxidative stress in the mode of action of ALS inhibiting herbicides. Two populations of Amaranthus palmeri S. Watson from Spain (sensitive and resistant to nicosulfuron, due to mutated ALS) were subjected to nicosulfuron treatment at different rates. The results showed that oxidative stress is linked to ALS inhibition, but a supralethal nicosulfuron rate was needed to induce a significant oxidative stress response in the sensitive population, indicating that ALS inhibitors' lethality is not entirely dependent on oxidative stress.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Cell Biology
Krzysztof Berezka, Marta Semkiv, Mariia Borbuliak, Johanna Blomqvist, Tomas Linder, Justyna Ruchala, Kostyantyn Dmytruk, Volkmar Passoth, Andriy Sibirny
Summary: The study aimed to isolate insertional mutants of S. stipitis with altered ethanol production and found that in one of the best fermenting strains, insertion occurred within a gene homologous to the yeast gene HEM25.
CELL BIOLOGY INTERNATIONAL
(2021)
Article
Cell Biology
Olena Dmytruk, Nina Bulbotka, Anastasya Zazulya, Marta Semkiv, Kostyantyn Dmytruk, Andriy Sibirny
Summary: Enzymes involved in methanol metabolism of methylotrophic yeasts are localized in peroxisomes and cytosol, with a decrease in activity observed after shifting cells from methanol to glucose medium. The mechanism of cytosolic enzyme inactivation remains unknown, with studies showing protein degradation as a cause in some strains. Autophagy pathway is found to play a role in degradation of certain enzymes in glucose medium, suggesting a complex regulatory mechanism in methanol metabolism.
CELL BIOLOGY INTERNATIONAL
(2021)
Review
Microbiology
Justyna Ruchala, Andriy A. Sibirny
Summary: The paper discusses the distribution of pentose sugars in nature, as well as the metabolism and conversion processes of D-xylose and L-arabinose. It provides a detailed exploration of pentose metabolism and bioconversion in non-conventional yeasts, and compares pentose metabolism in recombinant strains. The article also reviews genome studies of natural xylose-metabolizing yeasts and the metabolism of other pentoses.
FEMS MICROBIOLOGY REVIEWS
(2021)
Article
Microbiology
Ljubov Dzanaeva, Barbara Kruk, Justyna Ruchala, Andriy Sibirny, Kostyantyn Dmytruk
Summary: Lignocellulosic biomass is a sustainable platform for fuel ethanol production, with xylose being the second most abundant sugar in hydrolysates. Engineered Saccharomyces cerevisiae strains can ferment xylose, but the yield and productivities are lower compared to glucose fermentation. A study on transcription factors Znf1, Sip4, Adr1, Tup1, and Hap4 showed that Hap4 has a positive impact on xylose growth and fermentation, with the hap4 Delta mutant displaying increased ethanol production.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Justyna Ruchala, Yuliia A. Andreieva, Andriy O. Tsyrulnyk, Svitlana M. Sobchuk, Alicja Najdecka, Liu Wen, Yingqian Kang, Olena Dmytruk, Kostyantyn Dmytruk, Dariya Fedorovych, Andriy A. Sibirny
Summary: The study showed that engineered strains of Candida famata can produce high levels of riboflavin using whey as a substrate. Through genetic modifications and optimization of cultivation conditions, riboflavin production on whey was significantly increased, providing potential for producing high-value products from waste in the dairy industry.
MICROBIAL CELL FACTORIES
(2022)
Article
Biotechnology & Applied Microbiology
Marta Semkiv, Justyna Ruchala, Aksynia Y. Tsaruk, Anastasiya Z. Zazulya, Roksolana V. Vasylyshyn, Olena Dmytruk, MingXing Zuo, Yingqian Kang, Kostyantyn Dmytruk, Andriy A. Sibirny
Summary: This study highlights the important roles of hexose sensor Hxs1 and transcription factor Azf1 in xylose and glucose alcoholic fermentation in the native xylose-fermenting yeast O. polymorpha. Overexpression of these genes can significantly increase ethanol production during fermentation.
MICROBIAL CELL FACTORIES
(2022)
Article
Chemistry, Analytical
Kseniia Berketa, Olga Saiapina, Lyubov Fayura, Andriy Sibirny, Sergei Dzyadevych, Oleksandr Soldatkin
Summary: A novel arginine-sensitive conductometric biosensor based on a recombinant arginine deiminase (ADI) produced in E. coli was developed for the detection of L-arginine. The biosensor exhibited high selectivity and stability, making it suitable for quantification of arginine in dietary supplements.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biochemical Research Methods
Yuriy Kit, Marina Starykovych, Nazar Manko, Andrii Orfin, Tamila Alexanyan, Lydmyla Bozhko, Tetiana Turchyna, Oleg Kit, Roopesh Krishnankutty, Anjana Anand, Andrey Sibirny, Serhiy Souchelnytskyi, Rostyslav Stoika
Summary: Similar immune responses were observed in Covid-19 and autoimmune patients. A truncated form of unconventional Myosin 1C (48/Myo1C) was detected in the blood serum of autoimmune disease patients and severely ill Covid-19 patients, and its levels were increased by dexamethasone treatment. The 48/Myo1C protein suppressed the viability of activated lymphocytes and potentially interacted with components of the complement and coagulation systems.
JOURNAL OF IMMUNOLOGICAL METHODS
(2023)
Article
Veterinary Sciences
Oleh M. Fedets, Kostyantyn V. Dmytruk, Lukasz Adaszek, Iryna M. Kurlyak, Olena V. Dmytruk, Urszula Lisiecka, Stanislaw Winiarczyk
Summary: This study found an association between single nucleotide polymorphisms (SNPs) in the glutathione S-transferase P1 (GSTP1) gene and mammary tumors in dogs. Dogs with mammary tumors showed significant differences in GSTP1 SNPs compared to healthy dogs. This suggests that GSTP1 SNPs may be associated with the occurrence of mammary tumors in dogs.
VETERINARY AND COMPARATIVE ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anastasiya Z. Z. Zazulya, Marta V. V. Semkiv, Maxim Stec, Zuzanna Cyske, Lidia Gaffke, Karolina Pierzynowska, Grzegorz Wegrzyn, Andriy A. A. Sibirny
Summary: The methylotrophic yeast Komagataella phaffii is an effective producer of recombinant proteins. The degradation of cytosolic recombinant proteins in this yeast is believed to occur partially through the autophagic pathway. A study was conducted to identify the factors influencing this degradation process, using a system for selecting strains with impaired autophagy. It was found that the gene ACG1, encoding beta-1,6-N-acetylglucosaminyltransferase, plays a role in autophagy of cytosolic and peroxisomal proteins.
Article
Biochemistry & Molecular Biology
Dariya V. Fedorovych, Andriy O. Tsyrulnyk, Justyna Ruchala, Svitlana M. Sobchuk, Kostyantyn V. Dmytruk, Lyubov R. Fayura, Andriy A. Sibirny
Summary: This study successfully achieved higher production of FMN coenzyme in lactose and cheese whey by genetic modification of yeast genes. The best recombinant strain achieved an accumulation of 540 mg/L FMN in cheese whey, providing a new approach for the industrial production of enzyme-catalyzed reactions.
Article
Biotechnology & Applied Microbiology
Yana Petrovska, Oleksii Lyzak, Justyna Ruchala, Kostyantyn Dmytruk, Andriy Sibirny
Summary: Riboflavin, a water-soluble vitamin, plays a vital role in energy metabolism. Candida famata yeast has the potential to be a promising producer of riboflavin, and the overexpression of certain structural genes can significantly increase riboflavin production.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Olena Kurylenko, Justyna Ruchala, Barbara Kruk, Roksolana Vasylyshyn, Justyna Szczepaniak, Kostyantyn Dmytruk, Andriy Sibirny
Summary: This study characterizes the role of transcriptional factors in the natural xylose-fermenting yeast O. polymorpha, finding that the deletion of MIG1 and MIG2 genes reduces ethanol production, while the deletion of HAP4-A and TUP1 genes increases ethanol production. Thus, HAP4-A and TUP1 are involved in repression of xylose metabolism and fermentation in yeast O. polymorpha, offering a potential strategy to improve ethanol production from pentose sugars.
FEMS YEAST RESEARCH
(2021)
