Journal
BIOSENSORS & BIOELECTRONICS
Volume 151, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.111998
Keywords
Nucleic acid testing; Paper microfluidics; Mosquito-borne diseases; Viral RNA extraction; RT-LAMP; Point-of-care diagnostics
Categories
Funding
- GIST (Gwangju Institute of Science and Technology), South Korea
- Bio & Medical Technology Development Program of the National Research Foundation (NRF), South Korea - Ministry of Science ICT [NRF-2015M3A9E2031375]
- Global Research Lab (GRL) Program, South Korea [NRF2013K1A1A2A02050616]
Ask authors/readers for more resources
The recent outbreaks of mosquito-borne diseases (e.g., zika, dengue, and chikungunya) increased public health burden in developing countries. To control the spread of these infectious diseases, a simple, economic, reliable, sensitive, and selective diagnostic platform is required. Considering demand for affordable and accessible methods, we have demonstrated a two-step strategy for extraction and detection of viral RNAs of infectious diseases within 1 h. Ready-to-use devices for viral RNA extraction and detection were successfully fabricated using paper as a substrate. Viral RNA (e.g., zika, dengue, and chikungunya) was captured and eluted using a handheld RNA extraction paper-strip device, and another paper-chip device was used for reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay with a detection limit of a single copy and 10 copies of viral RNA in phosphate buffer solution (PBS) and serum, respectively. With these proposed devices, we have detected viral RNAs of zika and dengue in clinical human serum samples. The proposed paper-based extraction and detection platforms could be employed for detection of infectious viral diseases from complex clinical samples in resource-limited settings.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available