Distance-dependent plasmon-enhanced electrochemiluminescence biosensor based on MoS2 nanosheets

Nonmetallic plasmonic MoS2 nanosheets were synthesized by hydrothermal top-down method. MoS2 nanosheets had shown strong surface plasmon coupling (SPC) light absorption in the visible and near-infrared region. Herein, the nonmetallic plasmonic MoS2 nanosheets were employed to enhance the electrochemiluminescence (ECL) signal of sulfur doped boron nitrogen QDs (S-BN QDs) in this work. It is important to regulate the distance between ECL luminophore and plasmonic nanoparticles. On one hand, too closed distance can cause energy or electron transfer, which could quench the ECL intensity of nano-luminophore. On the other hand, plasmonic nanostructure cannot significantly affect the luminescence in the far distance. Therefore, we discussed the distance-dependent plasmon-enhanced ECL in detail with different length DNA chains. Furthermore, we constructed a hybridization chain reaction (HCR) amplification ECL sensing mode with the SPC-ECL strategy. The proposed DNA sensor can quantify hepatitis C virus (HCV) gene from 0.5 pmoL/L to 1 nmoL/L with a limit of detection (LOD) of 0.17 pmoL/L.