Article
Chemistry, Analytical
Dongwei Wang, Jiasheng Wang
Summary: The study demonstrated the potential of functionalized polypyrrole nanowires as a platform for label-free miRNA detection using EIS, and constructed a sensitive and selective miRNA biosensor based on PPyNWs/PAMAM hybrid. The biosensor showed excellent sensing performances in monitoring DNA/miRNA hybridization, indicating the microstructure of the PPy nanowires array greatly improved its performance.
Article
Chemistry, Multidisciplinary
Rossella Svigelj, Ivan Zuliani, Cristian Grazioli, Nicolo Dossi, Rosanna Toniolo
Summary: Nanomaterials, particularly gold-based nanostructures, can enhance the conductivity and performance of electrochemical sensors. By utilizing gold nanoparticles and truncated aptamers, an impedimetric biosensor was developed for quantifying gluten in hydrolyzed matrices, achieving a low limit of detection and successfully detecting gluten in real food samples.
Article
Multidisciplinary Sciences
B. Chakraborty, A. Das, N. Mandal, N. Samanta, N. Das, C. Roy Chaudhuri
Summary: This study focused on enhancing mass transport of analyte towards sensor surface using an electric field in a graphene-ZnO nanorods heterostructure, aiming to improve the performance of carcinoembryonic antigen detection platform. The system achieved a detection limit of 1 fg/ml in human serum, which is three orders of magnitude lower than existing attempts on PoC devices. The results demonstrate the potential and value of this approach.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Fangming Chen, Qian Guo, Qiaoyu Yang, Yao Meng, Mingrui Jiang, Jian Wang, De-Wen Zhang
Summary: Label-free electrochemical visualization of cancer cell apoptosis is achieved using a light-addressable electrochemical sensor. The study demonstrates that the AC photocurrent on apoptotic MCF-7 cells increases, indicating heterogeneity in apoptosis degrees. This new method provides potential applications in apoptosis-associated drug screening and efficacy evaluation.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Hibba Yousef, Yang Liu, Lianxi Zheng
Summary: This paper reviews the recent advances in label-free electrochemical aptasensors for thrombin detection, with a focus on the application of nanomaterials and nanostructures. The performance, advantages, and limitations of these aptasensors are summarized and compared, highlighting the impact of nanomaterials and nanostructures on sensor performance.
Article
Chemistry, Analytical
Rong Li, Xiaoxia Li, Liu Su, Hetong Qi, Xuanfeng Yue, Honglan Qi
Summary: An electrochemical aptasensor was developed for the determination of serotonin through specific binding and electrochemical measurement. The sensor, using a 57-mer aptamer, showed good selectivity and simplicity in detecting serotonin in the range of 1 to 100 mu M with a detection limit of 0.3 mu M. This work demonstrates the potential of combining aptamer recognition and serotonin oxidation for accurate and efficient detection.
Article
Chemistry, Analytical
Ya-Shin Yeh, Yi-Kuang Yen, Ragurethinam Shanmugam
Summary: This study developed a cost-efficient, disposable, and highly sensitive paper-based electrochemical biosensor that can detect the concentration of interferon-gamma (IFN-?), which is related to Tuberculosis. It has the potential to assist in disease diagnosis.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Funa Meng, Zhongxi Jiang, Yajing Li, Peina Zhang, Haisheng Liu, Yanli Sun, Xueliang Wang
Summary: A enzyme-free and label-free electrochemical sensor was developed for detecting PNK activity. The sensor utilized the catalytic characteristic of PNK on DNA phosphorylation to achieve detection. The method showed good linear correlation and high sensitivity, making it a potential tool for PNK concentration detection in biological research and clinical diagnosis.
Article
Chemistry, Analytical
Guiyin Li, HaiMei Li, Xinhao Li, Huapeng Huang, Huimin Bian, Jintao Liang, Zhide Zhou
Summary: A novel label-free electrochemical aptasensor for LDL detection was developed using molybdenum disulfide-gold nanoparticle-ferrocene-carboxylic acid nanosheets (MoS2-Au-Fc NSs) as signal amplification strategy. The aptasensor showed a linear correlation with logarithm LDL concentration from 0.001 to 100.0 μg/mL with R2 of 0.9914, and a low detection limit of 0.42 ng/mL. The LDL detected in actual serum samples exhibited good actual errors (0.73%-5.00%) and satisfactory relative standard deviations (RSDs) (0.04%-0.84%).
MICROCHEMICAL JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Patrawadee Yaiwong, Siriporn Anuthum, Padchanee Sangthong, Jaroon Jakmunee, Suwussa Bamrungsap, Kontad Ounnunkad
Summary: This study presents the development of a simple, reliable, highly sensitive, and label-free aptasensor based on polyethyleneimine-coated gold nanoparticles (PEI-AuNPs) for the quantification of alpha-fetoprotein (AFP). The aptasensor is fabricated using a disposable ItalSens screen-printed electrode (SPE) and detects AFP concentration through the electrochemical response of toluidine blue (TB) intercalating into the aptamer-modified electrode after binding with the target. The assay exhibits a linear range of detection of 10 to 50000 pg mL(-1) with a limit of detection (LOD) of 9.5 pg mL(-1) in human serum.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Sanaz Akbarzadeh, Habibollah Khajehsharifi, Saeedeh Hajihosseini
Summary: A novel, rapid, and label-free aptamer-based electrochemical biosensor was designed for the detection of oxytetracycline (OTC), utilizing a newly synthesized nanocomposite to enhance electrical conductivity of the electrode surface. The aptasensor showed high sensitivity, low detection limit, wide linear range, and good repeatability for measuring OTC content in milk samples. The results demonstrated the effectiveness of the aptasensor in real sample analysis.
Article
Chemistry, Analytical
Rong Zou, Shuang Wang, Chunyan Chen, Xiaoming Chen, Hang Gong, Changqun Cai
Summary: A fluorescence strategy based on MoS2 nanosheets and catalytic hairpin assembly was proposed for highly sensitive and selective detection of H5N1 DNA. The platform reduced background signal in the absence of target, but significantly enhanced fluorescence signal upon addition of target DNA.
Review
Food Science & Technology
Jing Yi Ong, Andrew Pike, Ling Ling Tan
Summary: This article discusses the issues of mycotoxins in food and feed, emphasizing the importance of early detection as a cost-effective method for ensuring food safety. The development of biosensors, including affinity columns and aptasensors, for the quantitative analysis of mycotoxins is highlighted. The review also covers the advancements in electrochemical transducers and aptasensors, with a brief introduction to commercially available mycotoxin detection kits.
Article
Food Science & Technology
Yujiao Hou, Nan Long, Boyu Jia, Xiaofang Liao, Minghan Yang, Lizhu Fu, Lidong Zhou, Ping Sheng, Weijun Kong
Summary: This study developed a conveniently fabricated label-free electrochemical aptasensor for ultrasensitive detection of ochratoxin A (OTA) in food. The aptasensor showed high sensitivity and specificity, with a linear relationship between resistance and OTA concentration. The sensor exhibited promising practical applications in food control for identifying hazardous contaminants.
Article
Chemistry, Analytical
Constanza J. Venegas, Luna Rodriguez, Paulina Sierra-Rosales
Summary: In this work, a label-free electrochemical aptasensor based on functionalized carbon nanotubes was developed for the detection of carbendazim (CBZ) in food samples. The aptasensor achieved covalent immobilization of the aptamer onto the carbon nanotubes through a carbodiimide reaction. When CBZ was detected, the immobilized aptamer underwent a conformational change, blocking the access of the redox mediator and resulting in a measurable decrease in the voltammetric response. The aptasensor demonstrated a linear detection range of 1.0 to 50.0 nM and a detection limit of 4.35 nM. This work provides a simple, rapid, sensitive, and selective biosensor for CBZ detection and quantification in food samples.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)