4.7 Article

Variation in tissue Na+ content and the activity of SOS1 genes among two species and two related genera of Chrysanthemum

Journal

BMC PLANT BIOLOGY
Volume 16, Issue -, Pages -

Publisher

BMC
DOI: 10.1186/s12870-016-0781-9

Keywords

Chrysanthemum morifolium; Compositae; SOS1; Functional characterization; Complementation assay

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Funding

  1. Chinese Government National Natural Science Foundation [31171987]
  2. Chinese Ministry of Education Program for New Century Excellent Talents in University [NCET-12-0890]
  3. Programs of Innovation and Entrepreneurship Talents of Jiangsu Province
  4. Priority Academic Program Development of Jiangsu Higher Education Institutions

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Background: Chrysanthemum, a leading ornamental species, does not tolerate salinity stress, although some of its related species do. The current level of understanding regarding the mechanisms underlying salinity tolerance in this botanical group is still limited. Results: A comparison of the physiological responses to salinity stress was made between Chrysanthemum morifolium 'Jinba' and its more tolerant relatives Crossostephium chinense, Artemisia japonica and Chrysanthemum crassum. The stress induced a higher accumulation of Na+ and more reduction of K+ in C. morifolium than in C. chinense, C. crassum and A. japonica, which also showed higher K+/Na+ ratio. Homologs of an Na+/H+ antiporter (SOS1) were isolated from each species. The gene carried by the tolerant plants were more strongly induced by salt stress than those carried by the non-tolerant ones. When expressed heterologously, they also conferred a greater degree of tolerance to a yeast mutant lacking Na+-pumping ATPase and plasma membrane Na+/H+ antiporter activity. The data suggested that the products of AjSOS1, CrcSOS1 and CcSOS1 functioned more effectively as Na+ excluders than those of CmSOS1. Over expression of four SOS1s improves the salinity tolerance of transgenic plants and the overexpressing plants of SOS1s from salt tolerant plants were more tolerant than that from salt sensitive plants. In addition, the importance of certain AjSOS1 residues for effective ion transport activity and salinity tolerance was established by site-directed mutagenesis and heterologous expression in yeast. Conclusions: AjSOS1, CrcSOS1 and CcSOS1 have potential as transgenes for enhancing salinity tolerance. Some of the mutations identified here may offer opportunities to better understand the mechanistic basis of salinity tolerance in the chrysanthemum complex.

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