Article
Polymer Science
Bas van Bochove, Dirk W. Grijpma
Summary: The study investigated the impact of pore characteristics on the tensile properties of porous network films, finding that elasticity modulus decreased with increasing porosity, regardless of pore size. Network films prepared by stereolithography showed slightly higher values of elasticity modulus, maximum tensile strength, and toughness compared to those prepared by the salt leaching method, possibly due to differences in diluent usage during the preparation process. Additionally, the presence of salt during crosslinking process may have contributed to a less efficient crosslinking process.
EUROPEAN POLYMER JOURNAL
(2021)
Article
Polymer Science
Mike A. Geven, Anna Lapomarda, Olivier Guillaume, Christoph M. Sprecher, David Eglin, Giovanni Vozzi, Dirk W. Grijpma
Summary: Porous composites based on poly(trimethylene carbonate) and nano-hydroxyapatite were prepared by controlling the ethylene carbonate content, resulting in composites with 40 wt% nano-hydroxyapatite and 27 to 71% porosity. The surface structure of these composites was influenced by their porosity, with micro-pore channels and rough nano-scale surfaces dominating. Stiffness and toughness decreased with increasing porosity, and cell differentiation was affected by the porosity, showing a 3-fold reduction in calcium production on composites with 71% porosity compared to those with 27% porosity.
EUROPEAN POLYMER JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Honglei Kang, Xudong Jiang, Zhiwei Liu, Fan Liu, Guoping Yan, Feng Li
Summary: Biodegradable scaffolds based on biomedical polymeric materials were prepared using composite materials of PTMC/PLA/HA and PTMC/HA, showing low toxicity, good biodegradability, and biocompatibility while enhancing the cell multiplication ability of osteoblast cells.
Article
Polymer Science
Hiroaki Nobuoka, Rikyu Miyake, Jaeyeong Choi, Hiroaki Yoshida, Nalinthip Chanthaset, Hiroharu Ajiro
Summary: This research aims to create a library of ester-free trimetylene carbonate derivatives, with a focus on introducing cycloalkyl groups into the side chain to produce more versatile and high-performance polymers. The study found that different cycloalkyl groups can enhance the thermal properties of the polymer, with larger rings leading to an increase in glass transition temperature.
EUROPEAN POLYMER JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Bianca-Iustina Andreica, Daniela Ailincai, Andreea-Isabela Sandu, Luminita Marin
Summary: The paper introduces a new water-soluble biopolymer, poly(trimethylene carbonate) grafted chitosan, which shows promise for in vivo applications. By varying the molar ratio between chitosan and TMC, different chitosan derivatives with varied poly(trimethylene carbonate) chain content were synthesized. The study reveals that partial substitution of chitosan with poly(trimethylene carbonate) chains can lead to a water-soluble polymer with improved biocompatibility and higher biodegradability rate.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Engineering, Biomedical
Si-Yao Zheng, Zhi-Wei Liu, Hong-Lei Kang, Fan Liu, Guo-Ping Yan, Feng Li
Summary: Three-dimensional (3D)-printed scaffolds made of biodegradable polymers have been found to be effective in bone repair and regeneration, with low cytotoxicity, good biocompatibility, and the ability to enhance osteoblast proliferation.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Polymer Science
Zhipeng Hou, Siwen Chen, Wanruo Hu, Jing Guo, Peng Li, Jianshe Hu, Liqun Yang
Summary: This study prepared poly(trimethylene carbonate-co-2, 2'-dimethyltrimethylene carbonate) (P(TMC-co-DTC)) copolymers with different molecular weights or molar ratios and investigated their in vivo degradation behavior through implantation experiments in rats. The results showed that increasing the content of DTC segments in the copolymer reduced mass loss and enhanced form-stability, while increasing the molecular weight accelerated degradation rate and improved form-stability. The study provides promising carrier materials for long-acting drug delivery systems.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Yixiu Liu, A. Liang, Xu Li, Zhihe Ma, Dan Zhang
Summary: In this study, Ciprofloxacin-loaded PTMC implants were prepared to treat chronic osteomyelitis. The results showed that the implants sustained release of ciprofloxacin for 28 days and exhibited excellent antibacterial effects both in vitro and in vivo. The implants promoted healing of the affected bone and reduced inflammation, indicating their potential for clinical use.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jinchi Zhang, Xiaowei Zhang, Wei Li, Jing Guo, Liqun Yang, Guangqi Yan
Summary: In this study, PTMC/DH films were used for the first time to repair Achilles tendon defects. Different PTMC/DH films with DH content of 10%, 20%, and 30% were prepared and their drug release properties were investigated. The results showed that the PTMC/DH films released effective concentrations of doxycycline for more than 7 and 28 days in vitro and in vivo, respectively. The drug-loaded films also demonstrated good antibacterial activity and promoted the healing of Achilles tendon defects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Applied
Lee Yae Tan, Nalinthip Chanthaset, Arif Fadlan, Hiroharu Ajiro
Summary: Antimicrobial biocompatible polymers are highly valuable materials in medical technology, particularly in the context of the COVID-19 pandemic. This study synthesized ester-free derivatives of poly(trimethylene carbonate) (PTMC) with cinnamyl group and analyzed their molecular weight effects. The incorporation of cinnamyl derivatives into PTMC showed improved physical properties and suppressed the growth of E.coli and S.aureus.
REACTIVE & FUNCTIONAL POLYMERS
(2023)
Article
Polymer Science
Xiliang Liu, Song Liu, Kaiqi Li, Shaomin Feng, Youkun Fan, Lijun Peng, Xin Wang, Dongliang Chen, Chengdong Xiong, Wei Bai, Lifang Zhang
Summary: PTMC networks were prepared with three different crosslinking agents to improve mechanical properties and resist creep. The degradation characteristics of PTMC and PTMC networks were evaluated, showing different behaviors during degradation. PPL was found to effectively accelerate the degradation of PTMC networks.
POLYMER DEGRADATION AND STABILITY
(2021)
Article
Otorhinolaryngology
Benjamin C. Campbell, Taha Z. Shipchandler, Jonathan Y. M. Ting, Dominic Vernon, Rana S. Torabi, Manisha Miller, H. B. Harold Lee
Summary: This study describes a wraparound technique for nylon foil implant placement for large, isolated orbital floor fractures, which provides excellent results with low complication rates. The technique offers more support and stability than traditional implants for larger fractures.
AMERICAN JOURNAL OF OTOLARYNGOLOGY
(2022)
Article
Polymer Science
Aaron Perez, Gerard Lligadas, Juan Carlos Ronda, Marina Galia, Virginia Cadiz
Summary: Block copolymers containing poly(trimethylene carbonate) (PTMC) middle block and poly(furfuryl methacrylate) PFMA end blocks were synthesized and their thermal and mechanical properties were studied. Crosslinking via Diels-Alder (DA) reaction and thermoreversibility properties of the crosslinked polymer were investigated.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Polymer Science
Thomas Brossier, Gael Volpi, Vincent Lapinte, Sebastien Blanquer
Summary: The synthesis of semi-crystalline PTMC through ROP-amine shows promising results and compatibility with a variety of amines, offering a simple preparation method and the ability to monitor urethane bonds. The relationship between the nature of amines and the crystallinity of PTMC was discussed, along with the impact of crystallinity on the mechanical properties compared to amorphous PTMC.
Article
Biochemistry & Molecular Biology
Lihuang Wu, Yuqi Wang, Xinyue Zhao, Hongli Mao, Zhongwei Gu
Summary: Poly(trimethylene carbonate) (PTMC), a representative of biodegradable aliphatic polycarbonates, degrades in vivo via surface erosion. This unique degradation behavior and the resulting nonacidic products make it competitive with aliphatic polyesters in clinical practice. The biodegradation mechanism of PTMC involves macrophage-mediated erosion, where macrophages fuse into giant cells and release proinflammatory cytokines, primarily secreting enzymes (lipase specifically) to erode PTMC extracellularly.
Article
Engineering, Biomedical
Olivier Guillaume, Oliver Kopinski-Gruenwald, Gregor Weisgrab, Theresia Baumgartner, Aysu Arslan, Karin Whitmore, Sandra Van Vlierberghe, Aleksandr Ovsianikov
Summary: Tissue engineering and regenerative medicine (TERM) has previously relied on scaffold-based or scaffold-free strategies, but a new approach called the third TERM strategy, involving highly porous microscaffolds and single spheroids, has the potential to overcome limitations of previous options. A systematic study comparing microscaffold-based tissue units with conventional spheroids demonstrated that the microscaffold did not impede spheroid formation or impact viability. Additionally, the fusion and differentiation potential necessary for TERM applications were preserved. Microscaffold-based tissue units offer benefits such as enhanced cell retention, decreased compaction, and better size control for tissue constructs formed through self-assembly.
ACTA BIOMATERIALIA
(2023)
Article
Anatomy & Morphology
Charlotte Arand, Hansrudi Noser, Lukas Kamer, Dominic Gehweiler, Kirstin Handrich, Pol M. Rommens, Daniel Wagner
Summary: This study investigated the relation and interaction of constant pelvic parameters, such as pelvic incidence (PI) and acetabular orientation, in a group of 150 uninjured and bony healthy pelves. The results showed a positive correlation between PI and acetabular anteversion, and also found that European individuals had higher PI and acetabular parameters compared to the Asian group. These findings are important for developing more comprehensive treatment strategies for pelvic trauma or degenerative pathologies.
JOURNAL OF ANATOMY
(2022)
Article
Orthopedics
Lena Gens, Emma Marchionatti, Adrian Steiner, Martin J. Stoddart, Keith Thompson, Karen Mys, Stephan Zeiter, Caroline Constant
Summary: This study investigated the feasibility and suitability of autologous cancellous bone graft harvest from different anatomical donor sites in rats. The results showed that the tibia and iliac crest may be the most favorable donor sites, but there may be differences in microstructure and consistency of osteoprogenitor cell outgrowth among the donor sites.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Orthopedics
Caroline Constant, Thomas F. Moriarty, Daniel Arens, Brenna Pugliese, Stephan Zeiter
Summary: This study evaluated the impact of peri-anesthetic hypothermia in rodents on outcomes in preclinical orthopedic device-related infection (ODRI) studies. The results showed that hypothermia during surgery had a significant effect on post-mortem CFU counts from harvested tissues and implants, indicating that abnormal body temperature during bacterial inoculation should be considered to improve experimental reproducibility and decrease infection data variability.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Multidisciplinary Sciences
Firas Souleiman, Martin Heilemann, Robert Hennings, Pierre Hepp, Boyko Gueorguiev, Geoff Richards, Georg Osterhoff, Dominic Gehweiler
Summary: The aim of this study was to investigate the effect of different loading scenarios and foot positions on the configuration of the distal tibiofibular joint (DTFJ). The findings suggested that foot positioning had a significant effect on DTFJ configuration, while loading had no relevant effect on native ankles in neutral position.
SCIENTIFIC REPORTS
(2022)
Article
Engineering, Biomedical
Angela R. Armiento, Karen Mys, Keith Thompson, Maria Hildebrand, Dirk Nehrbass, Stephan Zeiter, David Eglin, Martin J. Stoddart, Luan P. Hatt, Werner E. G. Mueller
Summary: Understanding the optimal conditions for bone healing is crucial for addressing non-unions and large bone defects. In this study, a tissue engineered complex combining bioactive factors, regenerative progenitor cells, and biomaterials was tested in vitro and in vivo. The results showed enhanced gene expression and mineral deposition in the biomaterials in vitro, but limited ectopic bone formation and no enhanced bone healing in vivo. The study highlights the discrepancy between in vitro and in vivo outcomes and calls for more complex in vitro models for translational studies.
ACTA BIOMATERIALIA
(2023)
Article
Emergency Medicine
Torsten Pastor, Philipp Kastner, Firas Souleiman, Dominic Gehweiler, Filippo Migliorini, Bjorn-Christian Link, Frank J. P. Beeres, Reto Babst, Sven Nebelung, Bergita Ganse, Carsten Schoeneberg, Boyko Gueorguiev, Matthias Knobe
Summary: This study compared the distances between four different long plate designs and adjacent anatomical structures when using the MIPO technique. The results showed that using 45 degrees and 90 degrees helical plates as well as ALPS implants in MIPO was safely feasible, and they had significantly larger distances to the radial nerve compared to straight plates. However, attention should still be paid to the musculocutaneous nerve and the brachial artery.
EUROPEAN JOURNAL OF TRAUMA AND EMERGENCY SURGERY
(2023)
Article
Orthopedics
Jordy Schol, Daisuke Sakai, Takayuki Warita, Tadashi Nukaga, Kosuke Sako, Sebastian Wangler, Shota Tamagawa, Stephan Zeiter, Mauro Alini, Sibylle Grad
Summary: This study found that vertebrally transplanted MSCs have a beneficial effect on intervertebral disc degeneration, suggesting a potential alternative treatment strategy.
Article
Multidisciplinary Sciences
Slobodan Tepic, Daniel Arens, Tim Buchholz, Dirk Nehrbass, Olivera Cvetkovic, Martin J. Stoddart, R. G. Richards, Stephan Zeiter
Summary: Pneumonia, especially during the COVID-19 pandemic, has become a major public health and economic disaster. This study suggests that lung metabolism in inflammation may have common features regardless of the underlying cause. By comparing mice with bleomycin-induced lung inflammation to control groups, it was found that certain amino acids had significantly lower concentrations in the arterial blood, indicating that arginine replacement therapy may be beneficial in pneumonia.
Article
Endocrinology & Metabolism
Jan Barcik, Manuela Ernst, Tim Buchholz, Caroline Constant, Karen Mys, Devakara R. Epari, Stephan Zeiter, Markus Windolf
Summary: This study compares the effects of immediate and delayed application of mechanical stimulation on fracture healing in a large animal model. The results show that immediate stimulation promotes fracture callus development, while delayed stimulation slows down the healing progress.
Article
Medicine, General & Internal
Matthias Troeltzsch, Stephan Zeiter, Daniel Arens, Dirk Nehrbass, Florian A. Probst, Paris Liokatis, Michael Ehrenfeld, Sven Otto
Summary: This study used a pig model to investigate the link between chronic oral infections (periodontitis) and medication-induced osteonecrosis of the jaw (MRONJ). The results showed that oral infections can trigger MRONJ without prior dental surgical interventions, thus ruling out the decisive role of oral mucosal trauma in MRONJ pathogenesis.
MEDICINA-LITHUANIA
(2023)
Article
Biochemistry & Molecular Biology
Charlotte Wittmann, Niels Vanvelk, Anton E. E. Fuerst, T. Fintan Moriarty, Stephan Zeiter
Summary: This study presents a mouse model of orthopedic-device-related infection, using titanium discs infected with Staphylococcus aureus, to evaluate different inocula and methods of infection prevention. Low-dose and high-dose cefazolin administration systemically and antibiotic-loaded bone cement discs were tested for their efficacy in infection prevention. The results showed that high-dose perioperative antibiotic prophylaxis and antibiotic-loaded bone cement discs were highly effective in preventing infection.
Article
Medicine, General & Internal
Markus Windolf, Viktor Varjas, Dominic Gehweiler, Ronald Schwyn, Daniel Arens, Caroline Constant, Stephan Zeiter, Robert Geoff Richards, Manuela Ernst
Summary: The study aims to investigate the safety and performance of the Fracture Monitor device in animal models and explore the application of continuous implant load monitoring in assessing fracture healing. The results show that the sensor system can autonomously function for a certain period of time without any adverse effects. The relative implant loads at 4 and 8 weeks post-operation are significantly correlated with radiographic scores and normalized bone volume metrics.
MEDICINA-LITHUANIA
(2022)
Article
Medicine, General & Internal
Philipp Poxleitner, Pit J. Voss, David Steybe, Lisa-Marie Seibert, Stephan Zeiter, Martin J. Stoddart, Rainer Schmelzeisen, Sven Otto
Summary: This study evaluated the effects of bisphosphonate (BP) administration on tooth growth, and found that weekly administration of zoledronate (ZOL) in minipigs impacted tooth growth in all measuring axes.
MEDICINA-LITHUANIA
(2022)
Article
Orthopedics
M. Hildebrand, M. Herrmann, F. Gieling, D. Gehweiler, D. Mischler, S. Verrier, M. Alini, S. Zeiter, K. Thompson
Summary: This study aimed to develop and characterize a rat non-union model using internal plate fixation and microCT evaluation. The results showed that microCT could accurately predict bone formation capacity at early time points, and that reducing plate thickness improved bone healing.
ARCHIVES OF ORTHOPAEDIC AND TRAUMA SURGERY
(2022)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.