Article
Fisheries
Huijing Cui, Cong Cong, Lili Wang, Xiaoyu Li, Jibin Li, Huan Yang, Shuying Li, Yongping Xu
Summary: This study discovered a lytic phage named PETp9 which can effectively prevent and control Edwardsiella tarda infection in turbot. The best preventive effect was achieved after 7 days of feeding phage with MOI = 10, and the most effective treatment in the phage-injection therapy was when the phage with MOI = 10 was injected intraperitoneally immediately after the challenge. In phage-immersion therapy, the best therapeutic effect was obtained when the infected turbot were immersed in phage-seawater mixture (MOI = 10) for 3 hours. Furthermore, the activities of non-specific immune-related enzymes in the blood of turbot treated with phage gradually reached normal levels.
AQUACULTURE RESEARCH
(2022)
Article
Infectious Diseases
Yuying Fu, Lishan Zhang, Huanhuan Song, Junyan Liao, Li Lin, Wenjia Jiang, Xiaoyun Wu, Guibin Wang
Summary: This study investigates the role of lysine acetylation and succinylation in the antibiotic resistance mechanism of Edwardsiella tarda. The results demonstrate that these post-translational modifications play important roles in bacterial antibiotic resistance and are enriched in various metabolic pathways. The findings provide insights into the mechanism of antibiotic resistance and may aid future research on the pathogenesis of this bacterium.
Article
Fisheries
M. S. Nithin, S. K. Girisha, K. B. Kushala, D. V. Chandan, T. G. Puneeth, B. T. Naveen Kumar, T. N. Vinay, T. Suresh, Lopamudra Sahoo, K. S. Ramesh
Summary: Motile Aeromonas Septicemia, caused by Aeromonas hydrophila, has led to significant economic losses in aquaculture. The study identified a virulent strain of A. hydrophila with multidrug resistance and biofilm production, posing a threat to fish health in aquaculture. Novel lytic bacteriophages were isolated as a potential alternative to antibiotics for controlling A. hydrophila infections.
Review
Biochemistry & Molecular Biology
Xianghui Li, Yuhua He, Zhili Wang, Jiacun Wei, Tongxin Hu, Jiangzhe Si, Guangzhao Tao, Lei Zhang, Longxiang Xie, Abualgasim Elgaili Abdalla, Guoying Wang, Yanzhang Li, Tieshan Teng
Summary: The emergence of antibiotic resistance is a major challenge for global health, leading to an urgent need for alternative therapeutic strategies due to a shortage of antibiotic pipelines. Phage-antibiotic synergy (PAS) has emerged as a promising approach to combat antibiotic-resistant pathogens, by enhancing bacterial killing and reducing the development of antibiotic resistance through a combination of phages and antibiotics. Additionally, the genetic and molecular mechanisms of phage antibiotic synergy, as well as the potential of engineered phages in reducing bacterial pathogenicity, are addressed in this review.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Biochemical Research Methods
Chao Wang, Xue-sa Dong, Yan-yan Yang, Guo-jing Xu, Meng-meng Wu, Fa-jun Yan, Long-gang Zhang, Li An, Pei-sheng Fu, Xi-rong Wang, Yu-bin Su, Qing-lei Meng
Summary: Metabolites in the TCA cycle increase the viability of bacteria against chloramphenicol, while NADPH promotes antibiotic resistance and oxidants restore the bactericidal ability. Additionally, the intracellular redox state determines the sensitivity to chloramphenicol, and the total antioxidative capacity is significantly reduced in antibiotic-resistant strains. These findings suggest that metabolite adjuvants should be cautiously applied, as they promote resistance to antibiotics.
JOURNAL OF PROTEOME RESEARCH
(2021)
Review
Chemistry, Medicinal
Ahmad Y. Hassan, Janet T. Lin, Nicole Ricker, Hany Anany
Summary: Bacteriophages have emerged as a legitimate alternative antibacterial agent, with the potential to contribute greatly to bacterial evolution through interactions with mobile genetic elements. They also show promise in therapeutic and biocontrol applications in medical, agricultural, and food processing settings, as well as in the development of rapid, sensitive, and specific biosensors for various bacterial targets. Their bioengineering capabilities hold great potential in improving adaptability and effectiveness as biocontrol and detection tools.
Article
Immunology
Julia Lopez-Perez, Jennifer Otero, Miquel Sanchez-Osuna, Ivan Erill, Pilar Cortes, Montserrat Llagostera
Summary: This study investigates the emergence of Salmonella strains with reduced susceptibility to a cocktail of three phages in different scenarios. The results show that the mechanisms of resistance vary in different environments, but they do not significantly impair the success of Salmonella biocontrol and oral phage therapy.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Microbiology
Mark B. Carascal, Donna May dela Cruz-Papa, Roland Remenyi, Mely Cherrylynne B. Cruz, Raul V. Destura
Summary: Southeast Asia is a hotspot for antimicrobial resistance, and phage therapy has emerged as a promising alternative for treating multidrug-resistant infections. This narrative review highlights the current understanding of phages and presents a three-phase strategy to encourage a phage revolution in the clinical setting of Southeast Asia. The goal is to open avenues for scientific and policy-based discussions on phage therapy and combat the threat of multidrug-resistant pathogens.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Na Li, Yigang Zeng, Rong Bao, Tongyu Zhu, Demeng Tan, Bijie Hu
Summary: Klebsiella pneumoniae is a major pathogen causing both hospital-acquired and community-acquired infections, with the emergence of multidrug-resistant strains posing challenges to treatment. Phage therapy has garnered attention as a potential solution to combat MDR K. pneumoniae, although host bacteria may develop mechanisms to resist phages.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Michael Parcey, Steven Gayder, Alan J. Castle, Antonet M. Svircev
Summary: This study analyzed recently published genomes to find that two significant groups of E. amylovora are poorly identified using current CRISPR-based tracking methods. The study also demonstrated that the CRISPR-Cas system and an unidentified mechanism work together to provide a significant degree of resistance against one of the phages proposed for phage-based biocontrol.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Bei-bei Yan, Xue-sa Dong, Jun-peng Wang, Xiao-ying Li, Li An, Xi-rong Wang, Long-gang Zhang, Qing-lei Meng, Chao Wang
Summary: This study reveals that glutamate can promote chloramphenicol resistance by influencing glutathione biosynthesis and the tricarboxylic acid cycle. These findings are important for advancing our understanding of the impact of glutamate on antibiotic resistance.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Immunology
Jiao Xiang, Min-yi Li, Hui Li
Summary: Metabolic reprogramming using aspartate as a reprogramming metabolite offers an antibiotic-free approach to combat both tetracycline-sensitive and -resistant bacteria, potentially enhancing host protection.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Golshan Shakeri, Jens A. Hammerl, Abdollah Jamshidi, Kiarash Ghazvini, Manfred Rohde, Istvan Szabo, Corinna Kehrenberg, Madeleine Plotz, Sophie Kittler
Summary: This study isolated and characterized six bacteriophages that infect food-derived Salmonella isolates, with phage VB_StyS-LmqsSP1 showing the potential for biocontrol by suppressing Salmonella growth and reducing bacterial titer. The phage demonstrated lytic efficacy under conditions prevalent in food production environments, making it a promising candidate for controlling Salmonella contamination on poultry.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Review
Microbiology
Anu Bala Jaglan, Taruna Anand, Ravikant Verma, Medhavi Vashisth, Nitin Virmani, B. C. Bera, R. K. Vaid, B. N. Tripathi
Summary: In the present scenario, the emergence of antimicrobial resistance is a global health challenge that is difficult to treat and causing high morbidity and mortality. The limited discovery of new antibiotics has led researchers to explore alternatives, such as bacteriophages. This review provides a comprehensive narrative on the important aspects of bacteriophages and discusses their potential as a therapeutic alternative, as well as their role in biocontrol against food-borne diseases.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Wattana Pelyuntha, Ruttayaporn Ngasaman, Mingkwan Yingkajorn, Kridda Chukiatsiri, Soottawat Benjakul, Kitiya Vongkamjan
Summary: This study evaluated an alternative strategy using phage-based treatment against Salmonella isolated from broiler production, showing a high prevalence of Salmonella spp. in bedding samples from broiler farms in eastern and southern Thailand. Multiple Salmonella phages were isolated from various water sources and found to effectively lyse several Salmonella serovars, leading to the conclusion that a phage cocktail can be an effective biocontrol method against Salmonella in broiler production and MDR serovars.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Arnaud Marchant, Pierre Van Damme, Stanley Plotkin, Pieter Neels, Maria Cristina Cassetti, Jakob Cramer, Marion F. Gruber, David Goldblatt, Deborah King, Wendy Hartig-Merkel, Joris Vandeputte
Summary: A meeting was held in February 2023 by international organizations to discuss correlates of protection against COVID-19, reviewing evidence and identifying knowledge gaps. Neutralizing antibodies and T lymphocytes are believed to play a significant role in protection, but further studies are needed to consolidate the evidence and evaluate other potential factors.
Article
Biochemical Research Methods
Neetu Raghav, Seema Parveen, Simleen Kaur, Selwyn A. Wilson David, Hyesuk Kong, James L. Kenney, Rajesh K. Gupta
Summary: This study evaluated the use of solid medium in sterility testing. Results showed that the sterility test using Schaedler blood agar (SBA) was more sensitive and faster in detecting microorganisms compared to the current compendial method, particularly in samples containing thimerosal. The sterility test using solid medium could be completed in 7 days without any additional technology.
Article
Biochemical Research Methods
Loushambam Samananda Singh
Summary: Antibiotic resistance has become a major concern for global public health, and phage therapy is seen as an alternative for treating multidrug resistant bacterial infections. Nano-emulsion encapsulation is described as the best approach to maintain phage efficacy.
