Journal
BIOLOGICAL & PHARMACEUTICAL BULLETIN
Volume 43, Issue 2, Pages 334-339Publisher
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.b19-00719
Keywords
benzoylaconitine; cytokine; signaling pathway; synovial cell
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Funding
- National Natural Science Foundation of China [81671226, 81403161]
- Key Scientific Research Project of Colleges and Universities in Henan Province [19A310020]
- Natural Science Foundation of Henan Province [162300410222]
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Benzoylaconitine (BAC), the main hydrolysate of aconitine, is a lower toxic monoester type alkaloid considered as the pharmacodynamic constituent in Aconitum species. In this study, the effects and mechanisms of BAC on production of inflammatory cytokines interleukin (IL)-6 and IL-8 were investigated in IL-1 beta-stimulated human synovial SW982 cells. The SW982 cells were incubated with BAC (0, 5 and 10 mu M) before stimulating with IL-1 beta (10 ng/mL). The results revealed that BAC suppressed gene and protein expression of IL-6 and IL-8 induced by IL-1 beta. BAC decreased activation of mitogen-activated protein kinase (MAPK) and phosphorylation of Akt. BAC also inhibited degradation of I kappa B-alpha, phosphorylation and nuclear transposition of p65 protein. The results demonstrate that BAC exerts an anti-inflammatory effect dependent on MAPK, Akt and nuclear factor-kappa B (NF-kappa B) pathways in human synovial cells stimulated with IL-1 beta, suggesting that BAC may be exploited as a potential therapeutic agent for rheumatoid arthritis (RA) treatment.
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