Biologically Benign Multi-functional Mesoporous Silica Encapsulated Gold/Silver Nanorods for Anti-bacterial Applications by On-demand Release of Silver Ions

Although silver (Ag)-based nanoparticles (NPs) are frequently used for bactericidal purposes, they have critical issues including excessive release of Ag+, severe oxidation, and cytotoxicity. In this study, we designed a multifunctional, on-demand antibacterial agent by successively encapsulating bimetallic gold/silver nanorods (Ag/AgNRs) with mesoporous silica (mSiO(2)) shells. Au/AuNRs were synthesized by coating Ag on AuNRs in a controlled manner, so that they exhibited a localized surface plasmon resonance peak in the near-infrared (NIR) region. When Au/AgNR@mSiO(2) NPs were irradiated with an NIR laser under optimal conditions (0.4 W/cm(2)), they generated a small amount of heat (40-45 C-o), which successively triggered the release of Ag+ and induced bacterial cell death. Here, mSiO(2) shells play critical roles because they not only protect Ag from oxidation but also prevent the burst release of Ag+ and improve biocompatibility of the antibacterial agent against normal cells. We found that this multifunctional bacterial agent effectively kills gram-negative Escherichia coli and gram-positive Staphylococcus aureus without significantly increasing the temperature of the medium. Au/AgNR@mSiO(2) NPs were also biologically benign with high biocompatibility against mammalian cells.