Article
Environmental Sciences
Qin-Qin Li, Qian-Qian Xiang, Li-Hong Lian, Zhi-Ying Chen, Xia Luo, Cheng-Zhi Ding, Li-Qiang Chen
Summary: The study found that exposure to silver nanoparticles can significantly alter 96 metabolites in fish gills, primarily affecting amino acid metabolism and energy metabolism. Silver nanoparticles specifically induce changes in 72 metabolites, enriching pathways such as aminoacyl-tRNA biosynthesis, ABC transporters, and the citrate cycle. On the other hand, exposure to silver supernatant ions causes specific changes in 14 metabolites, mainly interfering with sphingolipid metabolism in fish gills.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Biochemistry & Molecular Biology
Elif Kaval Oguz
Summary: This study investigated the effects of cypermethrin on the gills of Lake Van fish, revealing that cypermethrin caused stress, deterioration of osmoregulation, DNA damage, and histopathological damage in the gills.
CHEMISTRY AND ECOLOGY
(2022)
Article
Environmental Sciences
Le Zhang, Xiangsheng Hong, Saihong Yan, Jinmiao Zha
Summary: The study revealed that exposure to fenvalerate caused immunotoxicity in fish, with more severe pathological changes during bacterial infection post-exposure. Fish exposed to fenvalerate exhibited significant alterations in neutrophil and lymphocyte counts after P. fluorescens injection.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Sara dos Santos Almeida, Vinicius Silva Oliveira, Mariana Ribeiro Dantas, Leonardo Luiz Borges, Simone Maria Teixeira de Saboia-Morais, Thiago Lopes Rocha, Elisa Flavia Luiz Cardoso Bailao
Summary: The study revealed that acute exposure to environmentally relevant concentrations of BP-3 had a stronger impact on the gills than on the liver of Poecilia reticulata. Different concentrations of BP-3 were associated with distinct histopathological changes in the gills and liver.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Environmental Sciences
Tuhina Gayen, Anchal Tripathi, Usha Kumari, Swati Mittal, Ajay Kumar Mittal
Summary: This study aimed to investigate the impact of environmentally relevant concentrations of aspirin on the liver of Labeo rohita. The results showed that aspirin caused significant alterations in biochemical parameters, such as antioxidant enzyme activity and lipid peroxidation, as well as histopathological changes in the liver. These findings suggest that aspirin has toxic effects on fish and could be used as potential indicators of pharmaceutical toxicity in environmental biomonitoring.
Article
Environmental Sciences
Analia Ale, Juan M. Galdoporpora, Martin F. Desimone, Fernando R. de la Torre, Jimena Cazenave
Summary: This study deepened the knowledge about the fate of silver nanoparticles by evaluating the potential mitigation of humic acids on their toxic effects. It was found that oxidative responses were alleviated when humic acids were present in the media.
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
(2021)
Article
Environmental Sciences
Norhazwani Jaafar, Ahmad Azfaralariff, Syafiq M. Musa, Mazlan Mohamed, Abdul Hafidz Yusoff, Azwan Mat Lazim
Summary: The study found a widespread distribution of microplastics in commercial marine fishes from Malaysia waters, which could potentially lead to human exposure through fish consumption.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
Yi-Chuang Xu, Guang-Hui Liu, Yi-Huan Xu, Tao Zhao, Hua Zheng, Xiao-Ying Tan
Summary: The study investigated the toxicological effects of environmental concentrations of TC on the gills of tilapia, revealing that TC exposure damaged the gill structure and function, induced oxidative stress, affected inflammatory responses, and inhibited cell cycle, resulting in mitochondrial dysfunction and apoptosis. Transcriptomic analysis indicated the extensive influences of TC exposure on gill function, with the immune system being the main target.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Silvia Voci, Cristina Pangua, Maria Cristina Martinez-Oharriz, Paula Aranaz, Maria Collantes, Juan M. Irache, Donato Cosco
Summary: The study characterizes Brij O2-stabilized gliadin nanoparticles for potential oral drug delivery. Physico-chemical evaluations and in vivo studies in rats and Caenorhabditis elegans confirmed the nanoparticles' ability to accumulate in the mucosa and move along the digestive system. The nanoparticles showed antioxidant activity, decreased age-related lipofuscin pigment, and demonstrated a safe profile. These findings support the future application of these nanoparticles for oral delivery of bioactives.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Environmental Sciences
Maryam Mahjoubian, Akram Sadat Naeemi, Mehdi Sheykhan
Summary: The study assessed the toxic effects of different titanium dioxide nanoparticles on zebrafish, with results showing that silver-doped TiO2 NPs caused more severe gene expression alterations, lipid peroxidation, genotoxicity, and histological changes compared to pure TiO2 nanoparticles. The findings suggest that silver doping of TiO2 NPs significantly increases their toxicity and results in more histological lesions, apoptosis, and oxidative stress in adult zebrafish.
Article
Pharmacology & Pharmacy
Burcu Uner, Samet Ozdemir, Ecem Yildirim, Aylin Yaba, Cetin Tas, Melike Uner, Yildiz Ozsoy
Summary: Nanoformulations including solid lipid nanoparticles (SLN), nanostructured lipid carriers (NLC), and nanoemulsion (NE) of loteprednol etabonate (LE) were prepared to enhance its ocular bioavailability and reduce the risk of side effects. The results showed that these nanoformulations exhibited better performance in terms of particle size, zeta potential, and stability compared to the marketed product.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Mari C. Manas-Torres, Cristina Gila-Vilchez, Francisco J. Vazquez-Perez, Pavel Kuzhir, David Momier, Jean-Claude Scimeca, Arnaud Borderie, Marianne Goracci, Fanny Burel-Vandenbos, Cristina Blanco-Elices, Ismael A. Rodriguez, Miguel Alaminos, Luis Alvarez de Cienfuegos, Modesto T. Lopez-Lopez
Summary: The incorporation of magnetic nanoparticles in hydrogel matrices has expanded the materials' application in biomedical research by allowing remote modulation of physical properties, inducing anisotropy, and improving mechanical stability. The resulting magnetic short-peptide hydrogels exhibit enhanced mechanical properties, faster self-healing, good biocompatibility, and potential for minimally invasive biomedical applications.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Nicholas A. Bernier, James Teh, Derek Reichel, Joanne L. Zahorsky-Reeves, J. Manuel Perez, Alexander M. Spokoyny
Summary: Host-guest interactions are a growing research area where recent work has shown the ability to chemically manipulate both host and guest molecules. In this study, boron cluster derivatives were found to be encapsulated into iron oxide nanoparticles, with interactions not dependent on pH. This system was shown to be nontoxic and alter the biodistribution of guest molecules in mice.
Article
Biotechnology & Applied Microbiology
Elisa Assirelli, Paolo Caravaggi, Antonio Mazzotti, Francesco Ursini, Alberto Leardini, Claudio Belvedere, Simona Neri
Summary: This study aimed to improve the understanding of human cartilage response to cyclic mechanical compressive stimuli by using an experimental model combining biomechanical data with cartilage molecular information. The study showed the importance of considering the original cartilage anatomical position for a reliable biomolecular analysis of knee OA metabolism in response to mechanical stimulation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Francesca Giulimondi, Luca Digiacomo, Elisabetta Vulpis, Luisa Loconte, Gianmarco Ferri, Francesco Cardarelli, Daniela Pozzi, Alessandra Zingoni, Giulio Caracciolo
Summary: The study demonstrates that nanoparticles with a protein corona can be captured by immune cells and modulate immune responses by regulating the production of inflammatory factors. This finding provides new approaches for disease treatment and prevention.
Article
Chemistry, Analytical
M. Ventura, H. Cavaco, I Delgado, I Coelho, S. Gueifao, M. Martins, M. H. Costa, A. Matos, I Castanheira
Summary: This study aims to investigate the impact of wildfires on the environment and human health in European Mediterranean countries by analyzing the levels of inorganic elements in vegetables consumed by rural populations. The results showed that the levels of contaminants decreased one year after the fires, but potential hazards still exist.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Andrew Collins, Peter Moller, Goran Gajski, Sona Vodenkova, Abdulhadi Abdulwahed, Diana Anderson, Ezgi Eyluel Bankoglu, Stefano Bonassi, Elisa Boutet-Robinet, Gunnar Brunborg, Christy Chao, Marcus S. S. Cooke, Carla Costa, Solange Costa, Alok Dhawan, Joaquin de Lapuente, Cristian Del Bo, Julien Dubus, Maria Dusinska, Susan J. J. Duthie, Naouale El Yamani, Bevin Engelward, Isabel Gaivao, Lisa Giovannelli, Roger Godschalk, Sofia Guilherme, Kristine B. B. Gutzkow, Khaled Habas, Alba Hernandez, Oscar Herrero, Marina Isidori, Awadhesh N. N. Jha, Siegfried Knasmueller, Ingeborg M. M. Kooter, Gudrun Koppen, Marcin Kruszewski, Carina Ladeira, Blanca Laffon, Marcelo Larramendy, Ludovic Le Hegarat, Angelique Lewies, Anna Lewinska, Guillermo E. E. Liwszyc, Adela Lopez de Cerain, Mugimane Manjanatha, Ricard Marcos, Mirta Milic, Vanessa Moraes de Andrade, Massimo Moretti, Damian Muruzabal, Matjaz Novak, Rui Oliveira, Ann-Karin Olsen, Norah Owiti, Mario Pacheco, Alok K. K. Pandey, Stefan Pfuhler, Bertrand Pourrut, Kerstin Reisinger, Emilio Rojas, Elise Runden-Pran, Julen Sanz-Serrano, Sergey Shaposhnikov, Ville Sipinen, Karen Smeets, Helga Stopper, Joao Paulo Teixeira, Vanessa Valdiglesias, Mahara Valverde, Frederique van Acker, Frederik-Jan van Schooten, Marie Vasquez, Johannes F. F. Wentzel, Maciej Wnuk, Annelies Wouters, Bojana Zegura, Tomas Zikmund, Sabine A. S. Langie, Amaya Azqueta
Summary: The comet assay is a versatile method for detecting various types of DNA damage in individual eukaryotic cells. It has been widely used in research, industry, and regulatory agencies, and has been adopted as an in vivo test for genotoxicity. The present document provides consensus protocols for applying the comet assay to different cell types, species, and types of DNA damage.
Article
Environmental Sciences
Isabella Bramatti, Beatriz Matos, Neusa Figueiredo, Pedro Pousao-Ferreira, Vasco Branco, Marta Martins
Summary: PAHs, as persistent pollutants, are commonly found in the environment as complex mixtures. The interaction effects between compounds in a mixture may trigger different toxicological mechanisms, and mixtures of carcinogenic and non-carcinogenic PAHs can further enhance genotoxic effects. Therefore, assessing the toxicity of PAHs should take into account the composition of the mixtures.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Marine & Freshwater Biology
Isabel B. Oliveira, Ana Carvalhais, Sofia Guilherme, Helena Oliveira, Catarina C. V. Oliveira, Leonor Ferrao, Elsa Cabrita, Mario Pecheco, Claudia Mieiro
Summary: Studies have shown that titanium dioxide nanoparticles (TiO2 NP) have reproductive toxicity to humans and fish, but their effects on marine bivalves, specifically oysters, are still unknown. In this study, Pacific oyster sperm was directly exposed to two concentrations of TiO2 NP (1 and 10 mg/L) for a short term (1 h), and sperm motility, antioxidant responses, and DNA integrity were assessed. Although no changes were observed in sperm motility and antioxidant activities, the indicator of genetic damage increased at both concentrations, indicating that TiO2 NP affects the DNA integrity of oyster sperm. This vulnerability of Pacific oyster sperm to TiO2 NP highlights the importance of studying the effects of NP exposure on broadcast spawners.
AQUATIC TOXICOLOGY
(2023)
Article
Environmental Sciences
Claudia Leopoldina Mieiro, Joao Pedro Coelho, Ana Teresa Reis, Diana Castro, Paula Figueira, Filipe Martinho, Miguel A. A. Pardal, Eduarda Pereira, Mario Pacheco, Claudia B. Lopes
Summary: This study investigated the effects of biosorption on the health of turbot and found that remediation with non-living macroalgae significantly reduced the metallic burdens in the blood and kidney of the fish. In addition, no genotoxic potential was observed in the treated fish, indicating that biosorption remediation can successfully reduce metal loads while maintaining fish health.
Article
Microbiology
Katherine S. S. Marsay, Ana C. C. Ambrosino, Yuri Koucherov, Keren Davidov, Neusa Figueiredo, Iryna Yakovenko, Sheli Itzahri, Marta Martins, Paula Sobral, Matan Oren
Summary: Microplastic debris in marine environments is influenced by geographical location and season, affecting its physical characteristics and bacterial microbiome composition. This study collected microplastic samples from estuaries in the Mediterranean Sea and Atlantic Ocean, revealing significant variations between different locations and seasons. The findings highlight the importance of studying microplastic in diverse environments to understand its ecological impact and associated species.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Vitoria Pereira, Silvia F. S. Pires, Andreia C. M. Rodrigues, Pearl Ofoegbu, Pedro Bem-Haja, Amadeu M. V. M. Soares, Luis E. C. Conceicao, Rui J. M. Rocha, Mario Pacheco
Summary: Bivalve shellfish aquaculture is a sustainable and nutritious way to feed the growing global population. Microencapsulation technology has the potential to improve bivalve nutrition and enhance production quality. This study evaluated the food intake of five valuable bivalve species using algae-based microencapsulated diets, and found that all the tested diets were easily ingested, with some differences among species. This research contributes to the optimization of bivalve feed and the improvement of the final product.
Article
Biology
Fernanda Garcia, Antonio Alves da Silva, Kathreen Ruckstuhl, Peter Neuhaus, Catarina Coelho, Muyang Wang, Jose Paulo Sousa, Joana Alves
Summary: Male and female red deer exhibit sexual segregation, living separately except during mating season. Our study explores the diets of both sexes and suggests that diet may contribute to sexual segregation in a Mediterranean mountainous environment. The study highlights the importance of considering other factors that may impact sexual segregation in ungulate populations.
Article
Environmental Sciences
Giuseppe De Marco, Barbara Bille, Fatima Brandao, Mariachiara Galati, Patricia Pereira, Tiziana Cappello, Mario Pacheco
Summary: Mercury is a dangerous trace element that accumulates in biota. Dietary exposure to methylmercury (MeHg) can have differential effects on fish organs, as seen in a time-course experiment with juvenile white seabreams. The gills exhibited protein catabolism, disturbances in neurotransmission, and changes in osmoregulation and metabolism, while the liver showed altered protein metabolism and decreased amino acids, likely for defensive strategies. Post-exposure period allowed for partial recovery of both organs, but with oxidative stress and changes in energy metabolism. These organ-specific responses highlight the importance of considering selected organ in biomonitoring studies.
Article
Environmental Sciences
Fatima Fernandez-Garcia, Ana Carvalhais, Ana Marques, Isabel B. Oliveira, Sofia Guilherme, Helena Oliveira, Catarina C. V. Oliveira, Elsa Cabrita, Juan F. Asturiano, Mario Pacheco, Claudia Mieiro
Summary: The study aimed to evaluate the reproductive toxicity of different concentrations of silver nanoparticles (Ag NP) on Pacific oysters by assessing sperm quality. The results showed that both environmental and supra-environmental levels of Ag NP impaired sperm motility without affecting mitochondrial function or causing membrane damage. It is hypothesized that the toxicity of Ag NP is mainly attributed to adhesion to the sperm membrane and blockade of membrane ion channels. The presence of Ag in the marine ecosystem is of environmental concern as it may impact oyster reproduction.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Catarina C. V. Oliveira, Leonor Ferrao, Victor Gallego, Claudia Mieiro, Isabel B. B. Oliveira, Ana Carvalhais, Mario Pachedo, Elsa Cabrita
Summary: Silver nanoparticles have a mild effect on sperm motility in gilthead seabream and can alter the subpopulation structure of spermatozoa. This study exposed seabream sperm samples to titanium dioxide and silver nanoparticles and found that both nanoparticles significantly affected sperm motility and subpopulations. The reproductive toxicity effect was observed only at supra-environmental concentrations.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Biodiversity Conservation
Artur R. M. Serrano, Martim Baptista, Rui Carvalho, Mario Boieiro, Sara Mendes, Marie Bartz, Sergio Timoteo, Henrique M. V. S. Azevedo-Pereira, Carlos A. S. Aguiar, Antonio Alves da Silva, Joana Alves, Maria Jesus I. Briones, Paulo A. V. Borges, Jose P. Sousa, Pedro Martins da Silva
Summary: The Gorongosa National Park is a significant protected area in Africa, known for its biodiversity and ecology restoration efforts. However, there is limited research on the invertebrate biodiversity in the park. This study aims to assess the diversity of Caraboidea beetles and provide insights for conservation planning and climate change assessment.
BIODIVERSITY DATA JOURNAL
(2023)
Article
Environmental Sciences
Patricia Goncalves Cardoso, Hugo Morais, Daniel Crespo, Daniela Tavares, Eduarda Pereira, Miguel Angelo Pardal
Summary: This study investigates the seasonal characterization of mercury accumulation in three estuaries along the Portuguese coast. The results show higher mercury concentrations in Ria de Aveiro and Tagus estuary, posing potential risks to human health.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)