Journal
BIORESOURCE TECHNOLOGY
Volume 198, Issue -, Pages 709-716Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.09.079
Keywords
3-Hydroxypropionic acid; Glucose; Xylose; ptsG; xylR
Funding
- National Research Foundation of Korea (NRF) Grant - Ministry of Science, ICT & Future Planning [2013R1A2A2A01006590]
- R&D Program of MOTIE/KEIT [10049675]
- National Research Foundation of Korea [2013R1A2A2A01006590] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Escherichia coli expressing the Lactobacillus brevis dhaB(1)B(2)B(3) and dhaR(1)R(2) clusters and Pseudomonas aeruginosa aldhH was engineered to produce 3-HP from glucose and xylose via the glycerol biosynthetic pathway. Glycerol, a key precursor for 3-HP biosynthesis was produced by overexpression of the GPD1 and GPP2 genes from Saccharomyces cerevisiae. For relief of carbon catabolite repression, deletion of the chromosomal ptsG gene and overexpression of the endogenous xylR gene rendered engineered E. coli JHS01300/pCPaGGRm to utilize glucose and xylose simultaneously and to produce glycerol at 0.48 g/g yield and 0.35 g/L-h productivity. Finally, engineered E. coli JHS01300/pELDRR + pCPaGGRm produced 29.4 g/L of 3-HP with 0.54 g/L-h productivity and 0.36 g/g yield in a sugar-limited fed-batch fermentation. It was concluded that dual modulation of sugar transport and glycerol biosynthesis is a promising strategy for efficient conversion of glucose and xylose to 3-HP. (C) 2015 Elsevier Ltd. All rights reserved.
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