Article
Environmental Sciences
Jun Zhang, Shixia Liu, Chaoyang Hu, Xiaojiao Chen, Xue Sun, Nianjun Xu
Summary: This study found that different concentrations of L-arginine could significantly enhance the heat tolerance of Gracilariopsis lemaneiformis by reducing oxidative damage and activating antioxidant enzymes. Transcriptome analysis revealed upregulation of genes related to stress defense and metabolism, providing insights into the mechanisms of heat resistance in this marine red macroalgae.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Feng Qin, Xiaonan Zang, Guangqiang Shui, Zhendong Wang
Summary: The study on the molecular mechanism of Gracilariopsis lemaneiformis at low temperatures revealed thousands of differentially expressed genes after 6 and 24 hours of treatment, with key genes in various pathways showing significant changes. Additionally, the upregulation of heat shock proteins under low temperature stress may play a crucial role in cold resistance for G. lemaneiformis.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Shixia Liu, Jun Zhang, Chaoyang Hu, Xue Sun, Nianjun Xu
Summary: GABA positively influences the tolerance of Gracilariopsis lemaneiformis to high-temperature stress by improving growth rate and antioxidant enzyme activities, as well as helping maintain energy supply and metabolic homeostasis.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Plant Sciences
Haihong Chen, Yiyi Hu, Guanpin Yang, Pingping Li, Jingru Yin, Xiaoqing Feng, Qiong Wu, Jingyu Zhang, Baoheng Xiao, Zhenghong Sui
Summary: This study reports the existence of macropinocytosis in the red alga Gracilariopsis lemaneiformis, which is rarely reported in organisms with cell walls. It was found that endocytosis in this alga is driven by F-actin and involves the formation of ruffles and cups from the cell surface, followed by acidification and acquisition of digestive function by the endocytic vesicles.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Multidisciplinary Sciences
Pengbing Pei, Muhammad Aslam, Hong Du, Honghao Liang, Hui Wang, Xiaojuan Liu, Weizhou Chen
Summary: This study comprehensively analyzed the variation in epiphytic bacterial communities of Gracilariopsis lemaneiformis at different geographic locations and environmental factors. The findings revealed significant differences in epiphytic bacteria among various locations in China, with environmental factors such as nitrogen and phosphorus playing a key role in shaping the community composition of these bacteria.
SCIENTIFIC REPORTS
(2021)
Article
Environmental Sciences
Shixia Liu, Jun Zhang, Xue Sun, Nianjun Xu
Summary: Gracilariopsis lemaneiformis, an important commercial red macroalga, is facing impacts from global warming. This study found that exogenous polyamines (PAs) significantly improved heat tolerance, antioxidant capacity, and gene expression in G. lemaneiformis. Exogenous PAs also enhanced energy supply, redox homeostasis, and genome integrity under heat stress.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Plant Sciences
Xiaojiao Chen, Yue Yao Tang, Haodong Yin, Xue Sun, Xiaoqian Zhang, Nianjun Xu
Summary: Our study sequenced the full-length transcriptome of Gracilariopsis lemaneiformis using third-generation sequencing (Pacific Biosciences single-molecule real-time long-read sequencing, SMRT-Seq) to identify alternatively spliced transcripts and alternative polyadenylation (APA) sites. We generated 346,544 nonchimeric reads, from which 13,630 unique full-length transcripts were obtained. Over 3000 new alternative splicing (AS) events were identified compared to known gene models. We also identified genes with poly (A) sites, microRNAs, long noncoding RNAs, and novel genes in G. lemaneiformis. Validation experiments showed that abiotic stresses and phytohormones induced specific AS events and alterations to transcript ratios, and differential APA resulted in novel 3' ends. Cu stress inhibited the growth of G. lemaneiformis, but this inhibition was alleviated by ACC treatment. RNA-Seq analysis revealed differential alternative splicing (DAS) events and upregulation of transcripts involved in carbon fixation and oxidative phosphorylation with ACC treatment.
Article
Environmental Sciences
Yuling Yang, Wei Li, Yahe Li, Nianjun Xu
Summary: This study investigated the combined effects of ocean acidification and warming on the growth and photosynthetic performance of the economically important marine macroalga Gracilariopsis lemaneiformis. The results showed that high CO2 and high temperature decreased the growth rate of the macroalga, but the high temperature may counteract the negative effects of high CO2 and improve nitrogen content.
MARINE ENVIRONMENTAL RESEARCH
(2021)
Article
Fisheries
Xiaojiao Chen, YueYao Tang, Xue Sun, Xiaoqian Zhang, Nianjun Xu
Summary: Gracilariopsis lemaneiformis, an economically important seaweed in China, showed positive growth responses when treated with IAA, which led to enhanced rhizoid length and branching to improve growth rate. The application of IAA also activated various pathways related to hormone synthesis, signal transduction, and cell division, as well as metabolism, to promote growth and branch formation in Gracilaria lemaneiformis.
Article
Environmental Sciences
Zhongshan Zhang, Xinyue Li, Xiaogang Xu, Genxiang Mao, Xue Sun, Nianjun Xu, Xiaomei Wang
Summary: Gracilariopsis lemaneiformis polysaccharide (GP) has been found to exert protective effects in an aging mouse model by increasing antioxidant enzyme activity, reducing malondialdehyde levels, alleviating liver damage, and improving mild cognitive impairment. Furthermore, GP was found to modulate the composition and metabolism of the gut microbiota, suggesting its potential role in regulating oxidative stress.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Feng Qin, Guangqiang Shui, Zheng Li, Mengge Tu, Xiaonan Zang
Summary: This research investigates the response mechanism of the ubiquitin-protease pathway to various abiotic stresses in the economically important red macroalga, Gracilariopsis lemaneiformis. The results show that soluble protein and ubiquitin content significantly increased under most stresses. Transcript analysis revealed instant and significant up-regulation of key genes under different stresses, suggesting their important role in coping with adversity. These findings provide insights for selecting potential candidate genes for genetic transformation to obtain stress-resistant strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yongchang Su, Shicheng Chen, Jiashen Shen, Zhiwei Yi, Shuji Liu, Shuilin Cai, Nan Pan, Kun Qiao, Xiaoting Chen, Bei Chen, Min Xu, Suping Yang, Zhiyu Liu
Summary: Candidate peptides with novel ACE inhibitor activity were identified from hydrolysates of Gracilariopsis lemaneiformis by virtual screening. These peptides showed significant effects in lowering blood pressure in hypertensive rats, and molecular docking studies revealed their interaction with ACE. Among the peptides, SFYYGK, RLVPVPY, and YIGNNPAKG exhibited potent ACE inhibitory activities and reduced systolic blood pressure in hypertensive rats.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Environmental Sciences
Deguang Sun, Xueyou Zhou, Xue Sun, Nianjun Xu
Summary: With the need for new seaweed germplasm due to the expansion of seaweed culture and changes in global climate, understanding the process of reproductive development and its regulation in seaweed, specifically Gracilariopsis lemaneiformis, is of great importance. Through physiological assays and transcriptome technologies, this study analyzed the development of G. lemaneiformis tetrasporophytes in four phases. The results showed an increase in photosynthetic capacity and upregulation of gene expression related to light harvesting, photosynthesis, carbon fixation, starch and sucrose metabolism, and antioxidant enzymes during tetraspore formation and release. These findings provide insights into the molecular mechanism of G. lemaneiformis tetrasporogenesis and can contribute to the development of new germplasm and the seaweed culture industry.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Plant Sciences
Haojie Jia, Hong Du, Muhmmad Aslam, Tangcheng Li, Canqi Zheng, Xihui Xie, Nan Huang, Honghao Liang, Pengbing Pei, Ping Li, Weizhou Chen, Xiaojuan Liu
Summary: This study investigated the physiological and molecular response of Gracilariopsis lemaneiformis, a marine macroalga, to sulfate deficiency. The results showed that the growth rate of the algae was significantly reduced under sulfate deficiency, but photosynthesis was not affected. The cells of G. lemaneiformis utilized stored sulfur from vacuole to meet their nutritional requirements during sulfate deficiency. The pathway of sulfur metabolism was suppressed under sulfate deficiency in G. lemaneiformis.
PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Yueyao Tang, Xiaojiao Chen, Hao Zhang, Xue Sun, Nianjun Xu
Summary: Severe marine pollution caused by excessive copper has posed a great threat to marine biosystems. The role of ACC in alleviating copper stress in G. lemaneiformis was investigated in this study. ACC was found to inhibit the expression of genes encoding copper transport proteins, reduce copper accumulation and MDA contents, and relieve the inhibition of G. lemaneiformis growth caused by copper stress. Additionally, ACC stimulated jasmonate acid synthesis and activated pathways related to translation, photosynthesis, and nitrogen metabolism.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Fisheries
Hunter S. Bailey, Ashley N. Fincannon, Lee A. Fuiman
Summary: This study investigated the transfer of fatty acids from broodstock diets to eggs and the time needed for the eggs to equilibrate to the diet in Southern flounder. The findings indicate that different diet treatments significantly affect the fatty acid composition of eggs, and it takes 8-16 weeks for the eggs to adapt to changes in the adult diet. These results are important for improving broodstock diets and feeding protocols in stock-enhancement programs.
Article
Fisheries
Yu Liu, Mingtao Lei, Hector Victor, Yan Wang
Summary: This study confirms the feasibility of replacing raw fish diet with a formulated diet in commercial farming of large yellow croaker, which can increase fish survival and yield, and reduce feed cost and body lipid deposition.
Article
Fisheries
Xiaoyan Zhang, Lize San, Yucong Yang, Yuehong Tao, Jiangong Ren, Yufeng Liu, Zhongwei He, Jiashuo Di, Ziteng Pei, Guixing Wang, Jilun Hou
Summary: Gynogenesis is an effective technique for establishing homogeneous lines and confirming potential chromosomal mechanisms of sex determination in fish. This study successfully induced gynogenetic families in Thamnaconus modestus and established a complete method for gynogenesis induction.
Article
Fisheries
Julieta C. Martinelli, Megan Considine, Helen R. Casendino, Carolyn M. Tarpey, Isadora Jimenez-Hidalgo, Jacqueline L. Padilla-Gamino, Teri L. King, Lorenz Hauser, Steven Rumrill, Chelsea L. Wood
Summary: Shell-boring polychaetes have caused significant damage to mariculture operations worldwide, particularly in the US Pacific region. This study provides a comprehensive dataset on the prevalence, species distribution, and environmental associations of these parasites, highlighting the impact of season, culturing methods, and environmental factors on infestation.
Article
Fisheries
Juan Gao, Xueshan Li, Kangle Lu, Kai Song, Jian Zhang, Ling Wang, Chunxiao Zhang
Summary: This study investigated the effects of dietary protein levels on growth performance, antioxidant capacity, digestive enzyme activity, and metamorphosis rate of bullfrog tadpoles. The optimal dietary protein level for bullfrog tadpoles was estimated to be 42.49%-46.50% of the diet.
Article
Fisheries
Qiang Li, Lijun Duan, Dongsheng Jin, Yuxin Chen, Yirong Lou, Qianjin Zhou, Zhongjie Xu, Fangjie Chen, Hongxian Chen, Guizong Xu, Maocang Yan, Guanjun Yang, Jianfei Lu, Yanjun Zhang, Jiong Chen
Summary: This study developed a centrifugal microfluidic chip with on-chip RPA to detect five pathogenic microorganisms. The chip enabled the parallel analysis of six genetic markers from a single sample and allowed for the highly automated detection of multiple samples. Compared with PCR and DNA sequencing, the on-chip RPA assay showed high sensitivity and specificity for detecting multiple pathogens in shrimp aquaculture.
Article
Fisheries
Xingchen Huo, Pengxu Wang, Fengxia Zhao, Qian Liu, Qingqing Tian, Lingjie Tang, Maolin Lv, Zhaohui Wei, Chunrong Yang, Jianguo Su
Summary: Bacterial diseases in aquaculture ponds have negative effects on sustainable development. The use of composite I20 biotherapy has been shown to effectively treat bacterial diseases in bullfrog ponds, providing a new strategy for controlling bacterial diseases in aquaculture.
Article
Fisheries
Peng Yin, Takaya Saito, Per Gunnar Fjelldal, Bjorn Tharandur Bjornsson, Sofie Charlotte Remo, Sandeep Sharma, Rolf Erik Olsen, Kristin Hamre
Summary: This study aims to investigate the effect of seasonal changes in temperature and photoperiod on the redox metabolism of Atlantic salmon at the molecular level. The research findings suggest that the changing photoperiod and temperature influenced the redox metabolism of Atlantic salmon, highlighting the importance of oxidative status in the ecological implications.
Article
Fisheries
Nicholas Oppong Mensah, Jeffery Kofi Asare, Emmanuel Tetteh-Doku Mensah, Ernest Christlieb Amrago, Frank Osei Tutu, Anthony Donkor
Summary: This study investigates aquaculture farmers' preference for climate-smart aquaculture insurance products, the challenges they face, and their preferred insurance coverage. The results show that farmers prefer Climate-Induced Aquaculture Stock Mortality Insurance and the most significant constraint is the delay in claim settlement.
Article
Fisheries
Qiu-Ping Chai, Pei Wu, Wei-Dan Jiang, Yang Liu, Hong-Mei Ren, Xiao-Wan Jin, Lin Feng, Xiao-Qiu Zhou
Summary: The study found that appropriate levels of potassium diformate (KDF) can enhance the immune defense and mediate the inflammatory process in fish, possibly through the regulation of T cell differentiation via JAK-STAT and NF-kappa B signaling pathways.
Article
Fisheries
Tian Zhu, Haomin Jia, Haopeng Zhang, Yujing Xiao, Cui Han, Jiaxin Yang
Summary: Chicken manure has significant effects on the cultivation of Chlorella and rotifers. The optimal amount of chicken manure extract for continuous cultivation is 10 mLL-1. Chicken manure can promote the growth of Chlorella and improve the fatty acid composition, indirectly enhancing the growth condition and fatty acid content of rotifers.
Article
Fisheries
Zheng Luo, Yang Yu, Zhenning Bao, Fuhua Li
Summary: This study analyzed the heritability and genetic correlation of two growth traits in Pacific white shrimp and evaluated the genomic prediction using different genomic selection models. The results showed that the NeuralNet model had the highest prediction accuracy and better prospects for predicting shrimp growth traits.
Article
Fisheries
Alberto Ruiz, Ignasi Sanahuja, Karl B. Andree, Dolors Furones, Paul G. Holhorea, Josep A. Calduch-Giner, Jose J. Pastor, Marc Vinas, Jaume Perez-Sanchez, Sofia Morais, Enric Gisbert
Summary: Supplementing diets with a combination of spice oleoresins can reduce lipid accumulation and inflammation in farmed fish. The combination of spice oleoresins also has an immunomodulatory effect on the fish's intestinal immune system, potentially offering anti-inflammatory benefits.
Article
Fisheries
Beibei Zhao, Renhui Mei, Le Li, Di Hu, Lei Li
Summary: This study reveals that JfCXCL8_L1a and JfCXCL8_L1b have different immune pathways, and JfCXCL8_L1b plays a significant role in enhancing the adaptive immunity of T cell-dependent antigen.
Article
Fisheries
Yuhang Liu, Danying Cao, Nan Wu, Xuyang Zhao, Qingsong Zhu, Lian Su, Fatima Altaf, Qianqian Zhang, Haokun Liu, Yongming Li, Bruno Hamish Unger, Yingyin Cheng, Wanting Zhang, Aihua Li, Yaping Wang, Xiao-Qin Xia
Summary: Based on previous research, sinomenine has been found to protect mucosal immunity in farmed fish species by preventing intestinal pathological changes and regulating gene expression related to inflammation. It also enhances immune homeostasis and controls the growth of pathogenic bacteria.