H-2 dynamics in the soil of a H-2-emitting zone (Sao Francisco Basin, Brazil): Microbial uptake quantification and reactive transport modelling

Sandy kaolinite-rich soils, collected in a H-2-emitting circular depression (ca. 500 m in diameter), located in the Sao Francisco basin (Brazil), were exposed to H-2 gas concentrations in the 500-5000 ppm range for up to eight weeks. The samples were found to consume H-2 at a rate of approximately 0.05-0.1 mmol H-2/soil kg/day due to the microbial activity. DNA extraction from these soil samples before and after H-2 exposure, followed by Ribosomal Intergenic Spacer Analysis (RISA) and 16S rRNA gene amplicon sequencing, indicated that (i) the bacterial community is dominated by phyla that have been previously recognized to scavenge atmospheric H-2, and (ii) H-2 exposure leads to a significant modification of the bacterial community distribution. Measured H-2 uptake rates were fitted to the integrated form of the Michaelis-Menten equation and were further implemented in a 1-D reactive transport model. The model simulates gas-soil interactions in a 1-m vertical soil column, assuming homogeneous distribution of H-2-consuming bacteria. The evolution of the H-2 concentration in the unsaturated soil porosity along the column was simulated considering two different scenarios: a deep H-2 source (Case 1) and a biogenic surface source (Case 2). It was shown that, in the case of diffusion-dominated H-2 transport as considered in this study, bacterial activity will control the amplitude of the H-2 flux across the column. Moreover, we determined that bacterial activity can dramatically decrease the H-2 concentration in the soil porosity, by a factor of two compared to the source concentration. According to the simulation, the timeresolved concentration data collected in the Sao Francisco depression [Prinzhofer et al., 2019; International Journal of Hydrogen Energy] are consistent with the combination of a deep (Case 1) and a surficial biogenic (Case 2) H-2 source in this locality.