Journal
BIORESOURCE TECHNOLOGY
Volume 179, Issue -, Pages 243-248Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.12.019
Keywords
Benthic microalgal cultivation; Okadaic acid; Dinophysistoxin-1; Photobioreactor; Microalgal harvesting
Funding
- National Science & Technology Pillar Program of China [2013BAK12B00]
- National Basic Research Program of China [2011CB200901]
- Basic Scientific Fund for National Public Research Institutes of China [2014T05, 2012G32]
- Science Foundation for Youth Scholars of State Oceanic Administration of China [2012131]
- Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences [KF2012No3]
Ask authors/readers for more resources
In this study, the cultivation conditions of Prorocentrum lima, including temperature, nutrient concentration, photoperiod, and salinity were observed, and then an effective method for the large-volume cultivation of P. lima using a vertical flat photobioreactor was developed for the first time. The maximum cell concentrations and toxin contents of P. lima cultured in the photobioreactor were reached after a 35 days cultivation. Moreover, a step-wise double-sedimentation centrifugation method was used to harvest the microalgae cells, with the harvest rate of 89%. Toxin analysis of dry microalgal powder indicated that OA and DTX1 contents were 15.2 and 21.6 mg g(-1), respectively. These results verify that the culture method using the proposed photobioreactor is effective to massively produce DSP toxin-containing P. lima. This study may serve as a guide for the large-scale production of toxin-producing red-tide benthic microalgae. (C) 2014 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available