Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 59, Issue 10, Pages 3855-3858Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201911390
Keywords
enzymes; functional assays; lipids; mass spectrometry imaging; PLA(2)
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Funding
- Australian Research Council [DE160101142, DP160104025, DP150101715, DP190101486, LP140100832]
- Norwegian Research Council (FRIPRO-YRT Fellowship) [287462]
- UK Medical Research Council [MR/L01839X/1]
- Wellcome Trust [200517/Z/16/Z]
- Royal Society (Sir Henry Dale Fellowship) [200517/Z/16/Z]
- Australian Research Council [LP140100832] Funding Source: Australian Research Council
- MRC [MR/L01839X/1] Funding Source: UKRI
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Enzymes are central components of most physiological processes, and are consequently implicated in various pathologies. High-resolution maps of enzyme activity within tissues therefore represent powerful tools for elucidating enzymatic functions in health and disease. Here, we present a novel mass spectrometry imaging (MSI) method for assaying the spatial distribution of enzymatic activity directly from tissue. MSI analysis of tissue sections exposed to phospholipid substrates produced high-resolution maps of phospholipase activity and specificity, which could subsequently be compared to histological images of the same section. Functional MSI thus represents a new and generalisable method for imaging biological activity in situ.
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