Journal
ANALYTICAL BIOCHEMISTRY
Volume 592, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2020.113582
Keywords
MALDI-TOF MS; PCA; Whole-cell identification; Pathogenic bacteria
Funding
- National Natural Science Foundation of China [31601569]
- Social Development Project of Jiangsu Science and Technology Department [BE2016779]
- China Postdoctoral Science Foundation [2017T100375, 2016M600420]
- Fundamental Research Funds for the Central Universities [KJQN201731]
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The rapid and accurate identification of foodborne pathogenic bacteria is of great importance for human health. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) can be used to rapidly and sensitively identify microorganisms but is limited by the expensive protein databases available. In this study, we established a whole-cell method for the identification of foodborne pathogenic bacteria, using MALDI-TOF MS and principal component analysis (PCA), which did not use protein extractions or expensive protein databases. Thirty strains comprising six common foodborne pathogenic bacteria, namely, Shigella flexneri, Escherichia coll., Staphylococcus aureus, Salmonella enteritidis, Pseudomonas aeruginosa, and Listeria monocytogenes were analyzed using MALDI-TOF MS. The culture time, matrix, and spotting method were optimized based on peak intensity and deviation. A PCA was performed to analyze the mass spectrometry results of six samples and proved capable of identifying significant changes in those samples. It was found that directly applying MALDI-TOF MS analysis to whole-cell bacteria, without protein extraction, exhibited rich peak contents and a high level of reproducibility. MALDI-TOF MS combined with PCA is a promising method of rapidly identifying pathogens in food products.
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