4.5 Article

A sensitive H2O2 biosensor based on carbon nanotubes/tetrathiafulvalene and its application in detecting NADH

Journal

ANALYTICAL BIOCHEMISTRY
Volume 589, Issue -, Pages -

Publisher

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2019.113493

Keywords

Modified electrode; Carbon nanotubes; Hydrogen peroxide; Biosensor; NADH

Funding

  1. National Natural Science Foundation of China, China [61801256, U1806202]
  2. Shandong Province Key Research and Development Plan, China [2018GGX109016]
  3. Natural Science Foundation of Shandong Province, China [ZR2018ZC0129, ZR2019MEE051]

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Reduced nicotinamide adenine dinucleotide (NADH) plays a pivotal role in the electron-transfer chain of biological system. Analysis of many biological markers is based on the detection of the enzymatically generated NADH. In this paper, a sensitive hydrogen peroxide (H2O2) biosensor, fabricated by carbon nanotubes (CNTs)/tetrathiafulvalene (TTF)/horseradish peroxidase (HRP), was applied for detecting the NADH in a buffer containing methylene blue (MB) at low operating potential of - 0.3 V (vs. Ag/AgCl). Since the NADH could be oxidized by MB to release H2O2, the electrochemical biosensor enables to detect the NADH in the MB buffer. And the low working potential made the biosensor avoid the interference from other electroactive substances. Linear response ranges from 10 mu M to 790 mu M, with a sensitivity of 4.76 mu A mM(-1) and a detection limit of 1.53 mu M were obtained under the optimum conditions. The proposed sensor provided a promising approach for sensitively detecting the NADH.

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