Article
Medical Laboratory Technology
Anula Divyash Singh, Balakrishna Nagalla, Sreekanth Patnam, G. Satyanaryana, Ravi Andrews, Aswini Kumar Panigrahi, Soma Sekhar Mudigonda, Sanjay Maitra, Aravind Kumar Rengan, Manda Venkata Sasidhar
Summary: This study demonstrates the diagnostic potential of urinary extracellular vesicle-derived mRNA markers for T-cells and B-cells in allograft dysfunction after solid organ transplantation. The expression of T-cell and B-cell markers showed significant differences between healthy controls and AGD patients. The combination of T-cell and B-cell markers improves the sensitivity and specificity for detecting AGD.
CLINICA CHIMICA ACTA
(2023)
Review
Biochemistry & Molecular Biology
Liang Wu, Karin Boer, Wouter W. Woud, Suwasin Udomkarnjananun, Dennis A. Hesselink, Carla C. Baan
Summary: Urinary extracellular vesicles (uEVs) are linked to processes like infection and rejection after kidney transplantation, providing a non-intrusive way to access local protein, DNA, and RNA analytics. Studies have shown that specific markers such as kidney-specific and immune response-related markers can diagnose complications post-kidney transplantation. Further research on novel uEV markers is needed to better understand transplant dysfunction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Jan Novak, Tana Machackova, Jan Krejci, Julie Bienertova-Vasku, Ondrej Slaby
Summary: Congestive heart failure affects about 23 million people worldwide, and cardiac allograft transplantation is one of the last options for patients with terminal refractory heart failure. The prognosis of patients after heart transplantation is influenced by rejection and allograft vasculopathy, with monitoring requiring invasive and uncomfortable procedures. MiRNAs play a potential role in the pathophysiology of rejection and may be used in clinical practice for diagnosis and prediction of rejection occurrence.
Article
Urology & Nephrology
Christina Lai, Steven J. Chadban, Yik Wen Loh, Tony King-Tak Kwan, Chuanmin Wang, Julian Singer, Paula Niewold, Zheng Ling, Alanna Spiteri, Daniel Getts, Nicholas Jonathan Cole King, Huiling Wu
Summary: Inflammatory monocytes are a major component of acute kidney transplant rejection. This study investigates the therapeutic potential of immune-modifying nanoparticles (IMPs) in preventing kidney allograft rejection. The results show that IMPs significantly reduce acute rejection, improve kidney function, and inhibit inflammatory cell density and cytokine expression. However, IMPs do not offer protection against chronic rejection.
KIDNEY INTERNATIONAL
(2022)
Article
Multidisciplinary Sciences
Frederik Banch Clausen, Kristine Mathilde Clara Lund M. Jorgensen, Lasse Witt M. Wardil, Leif Kofoed M. Nielsen, Grethe Risum M. Krog
Summary: By using Droplet Digital PCR (ddPCR), we developed an assay setup for monitoring organ health. The results demonstrated that ddPCR has great sensitivity, high precision, and exceptional ability to quantify low levels of cfDNA. Further prospective studies are needed to determine the clinical utility of the proposed method.
Article
Surgery
Philip F. Halloran, Georg A. Bohmig, Jonathan S. Bromberg, Klemens Budde, Gaurav Gupta, Gunilla Einecke, Farsad Eskandary, Katelynn Madill-Thomsen, Jeff Reeve
Summary: The study found that certain injury elements in kidney transplant are selective for specific pathologic mechanisms, with Dimension 3 being a newly discovered dimension involving genes related to epithelial polarity.
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Article
Immunology
Tahm Spitznagel, Laurenz S. S. Matter, Yves L. L. Kaufmann, Jakob Nilsson, Seraina von Moos, Thomas Schachtner
Summary: HLA-derived epitope-mismatches can help predict histopathological changes of immune-related injury in kidney transplant recipients from the first indication biopsy to the follow-up biopsy.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Emilie Gaiffe, Mathilde Colladant, Maxime Desmaret, Jamal Bamoulid, Franck Leroux, Caroline Laheurte, Sophie Brouard, Magali Giral, Philippe Saas, Cecile Courivaud, Nicolas Degauque, Didier Ducloux
Summary: Unsupervised hierarchical clustering analysis was performed on pre-transplant immunological phenotype in 1113 renal transplant recipients, identifying three immune profiles correlated with clinical phenotypes. The memory immune cluster was associated with older age and diabetes, while the activated immune cluster was associated with younger age and previous exposure to CMV. Both were associated with an increased risk of acute rejection.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Urology & Nephrology
Elisabet Van Loon, Baptiste Lamarthee, Henriette de Loor, Amaryllis H. Van Craenenbroeck, Sophie Brouard, Richard Danger, Magali Giral, Jasper Callemeyn, Claire Tinel, Alvaro Cortes Calabuig, Priyanka Koshy, Ben Sprangers, Dirk Kuypers, Wilfried Gwinner, Dany Anglicheau, Pierre Marquet, Maarten Naesens
Summary: Molecular changes in kidney transplant injury, especially immune cell activation pathways, were studied in peripheral blood and kidney allograft samples, highlighting the importance of immune activation in kidney transplant pathology despite strong immunosuppressant treatment.
KIDNEY INTERNATIONAL
(2022)
Article
Surgery
Cyril P. Landstra, Michiel F. Nijhoff, Dave L. Roelen, Aiko P. J. de Vries, Eelco J. P. de Koning
Summary: Islet transplantation stabilizes glycemic control in patients with complicated diabetes mellitus, but rapid functional decline could be due to islet allograft rejection. Currently, there is no reliable method for assessing rejection and treatment protocols are lacking. This study aimed to identify diagnostic features of islet allograft rejection and evaluate the effectiveness of high-dose methylprednisolone treatment.
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Article
Surgery
Braulio A. Marfil-Garza, Rena L. Pawlick, Jake Szeto, Charles Kroger, Vikas Tahiliani, Joshua Hefler, Nidheesh Dadheech, Mathew M. Seavey, Jeffrey Wolf, Rahul R. Jasuja, A. M. James Shapiro
Summary: In vivo expansion of regulatory T cells (Tregs) through antibody-mediated stimulation shows potential in prolonging graft survival in organ transplantation. The use of TNFRSF25 antibodies enabled significant Treg expansion and correlated with improved graft survival.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Immunology
Qinfan Yao, Cuili Wang, Yucheng Wang, Wenyu Xiang, Yin Chen, Qin Zhou, Jianghua Chen, Hong Jiang, Dajin Chen
Summary: This study identified key differentially expressed genes (DEGs) in acute allograft rejection (AR) and found that they have the potential to be used as biomarkers for early-stage AR diagnosis. The expression of these genes can reflect the immune status of patients with AR.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Guillaume Coutance, Eva Desire, Jean-Paul Duong Van Huyen
Summary: Despite advances in immunosuppression, allograft rejection remains a significant complication after heart transplantation. The current invasive diagnostic method has limitations, thus innovative strategies are needed to improve the accuracy of diagnosis.
Review
Pharmacology & Pharmacy
Min Young Kim, Daniel C. Brennan
Summary: Significant progress has been made in the pathophysiology, diagnosis, and treatment of antibody-mediated rejection (ABMR) in recent decades, but effective treatment for chronic ABMR remains a challenge. Chronic ABMR has a distinct phenotype from active ABMR and is mainly caused by de novo donor specific antibodies (DSA), leading to progressive graft injury.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Medicine, General & Internal
Miriam C. Banas, Georg A. Bohmig, Ondrej Viklicky, Lionel P. Rostaing, Thomas Jouve, Lluis Guirado, Carme Facundo, Oriol Bestard, Hermann-Josef Groene, Kazuhiro Kobayashi, Vladimir Hanzal, Franz Josef Putz, Daniel Zecher, Tobias Bergler, Sindy Neumann, Victoria Rothe, Amauri G. Schwaeble Santamaria, Eric Schiffer, Bernhard Banas
Summary: The multicentric observational prospective study (PARASOL) aimed to validate a novel non-invasive test system for predicting renal allograft rejection. Results showed that out of the transplant recipients included in the trial, 19.3% exhibited rejection symptoms, 44.0% had normal renal tissue, and 36.7% had other kidney damage.
FRONTIERS IN MEDICINE
(2022)
Article
Endocrinology & Metabolism
Matthew R. Weir, Jagadish Gogate, C. V. Damaraju, Ricardo Correa-Rotter, Kenneth W. Mahaffey
Summary: A post hoc analysis of integrated data from the CANVAS Program and CREDENCE trial found that canagliflozin reduced the risk of major adverse cardiovascular events (MACE) in Hispanic patients with type 2 diabetes and high cardiovascular risk or nephropathy, without heterogeneity by baseline estimated glomerular filtration rate (eGFR).
DIABETES OBESITY & METABOLISM
(2022)
Article
Surgery
Benjamin E. Hippen, David A. Axelrod, Kennan Maher, Ruixin Li, Deepali Kumar, Yasar Caliskan, Tarek Alhamad, Mark Schnitzler, Krista L. Lentine
Summary: There is significant heterogeneity in COVID-19 vaccination mandate policies for transplant candidates in US transplant centers. While vaccination is encouraged, most centers have not mandated COVID-19 vaccination for candidates and living donors, citing administrative opposition, legal prohibitions, and concern about equity in access to transplants.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Urology & Nephrology
Lihong Bu, Gaurav Gupta, Akshta Pai, Sanjiv Anand, Erik Stites, Irfan Moinuddin, Victor Bowers, Pranjal Jain, David A. Axelrod, Matthew R. Weir, Theresa K. Wolf-Doty, Jijiao Zeng, Wenlan Tian, Kunbin Qu, Robert Woodward, Sham Dholakia, Aleskandra De Golovine, Jonathan S. Bromberg, Haris Murad, Tarek Alhamad
Summary: Routine monitoring of dd-cfDNA after kidney transplant can help identify subclinical allograft injury and intervene before clinical graft injury develops. This study found a significant correlation between elevated levels of dd-cfDNA and allograft rejection. Persistently elevated dd-cfDNA predicted a decline in kidney function. Therefore, routine monitoring of dd-cfDNA can be an important tool for early detection of graft injury.
KIDNEY INTERNATIONAL
(2022)
Article
Gastroenterology & Hepatology
Ramesh K. Batra, Venkatesh K. Ariyamuthu, Malcolm P. MacConmara, Gaurav Gupta, Ahmet B. Gungor, Bekir Tanriover
Summary: The outcomes of simultaneous liver-kidney transplantation using kidneys from donors with acute kidney injury have been studied. It was found that the presence of acute kidney injury did not significantly affect the success of liver and kidney transplantation. This suggests that selected kidneys with acute kidney injury can be considered for simultaneous liver-kidney transplantation.
LIVER TRANSPLANTATION
(2022)
Article
Immunology
Yasar Caliskan, David Axelrod, Alexis Guenette, Ngan N. Lam, Vivek Kute, Tarek Alhamad, Mark A. Schnitzler, Krista L. Lentine
Summary: The study surveyed 62 respondents from 19 countries, showing that most of them encourage vaccination for kidney transplant candidates on the waiting list and before planned living donor transplant. Practitioners in some countries are less willing to accept deceased donor organs for waitlist candidates with incomplete COVID-19 vaccination status and are more willing to wait longer before scheduling living donor transplant compared to United States practices.
TRANSPLANT INFECTIOUS DISEASE
(2022)
Letter
Immunology
Gaurav Gupta, Ambreen Azhar, Ahmet Gungor, Miklos Z. Molnar, Megan K. Morales, Bekir Tanriover
Letter
Immunology
Gaurav Gupta, Teja Devarakonda, Aadi Sharma, Madeleine Miles, Idris Yakubu, Aamir Khan, Richard K. Sterling
Article
Economics
Lauren Fusfeld, Sreeranjani Menon, Gaurav Gupta, Christopher Lawrence, Salwa F. Masud, Thomas F. Goss
Summary: This study evaluates the economic impact of using MMDx-Kidney in conjunction with histologic evaluation in kidney transplants, and finds that it improves the diagnosis of rejection and reduces associated costs.
JOURNAL OF MEDICAL ECONOMICS
(2022)
Article
Urology & Nephrology
Donald E. Hricik, Brian Armstrong, Tarek Alhamad, Daniel C. Brennan, Jonathan S. Bromberg, Suphamai Bunnapradist, Sindhu Chandran, Robert L. Fairchild, David P. Foley, Richard Formica, Ian W. Gibson, Karen Kesler, S. Joseph Kim, Roslyn B. Mannon, Madhav C. Menon, Kenneth A. Newell, Peter Nickerson, Jonah Odim, Emilio D. Poggio, Randall Sung, Ron Shapiro, Kathryn Tinckam, Flavio Vincenti, Peter S. Heeger
Summary: This study tested the effects of blocking TNFa during kidney transplantation. The results showed that there was no significant difference in eGFR at 24 months between the IFX and placebo groups, but the IFX group had higher rates of BK virus infection.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2023)
Article
Surgery
Siobhan Sutcliffe, Mengmeng Ji, Su-Hsin Chang, Darren Stewart, David A. Axelrod, Krista L. Lentine, Jason Wellen, Louai Alrata, Gaurav Gupta, Tarek Alhamad
Summary: In this study, we found that receiving a kidney transplant from a hepatitis C virus (HCV) positive donor did not increase the risk of transplant failure in recipients who were HCV-negative and treated with direct-acting antiviral medications. Kidneys from HCV-positive donors were associated with higher estimated glomerular filtration and a lower risk of delayed graft function compared to HCV-negative kidneys. These findings suggest that including donor HCV status in the Kidney Donor Risk Index may no longer be necessary in current practice.
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Review
Surgery
Ronald F. Parsons, Ekamol Tantisattamo, Wisit Cheungpasitporn, Arpita Basu, Yee Lu, Krista L. Lentine, Kenneth J. Woodside, Neeraj Singh, Joseph Scalea, Tarek Alhamad, Ty B. Dunn, Franco H. Cabeza Rivera, Sandesh Parajuli, Martha Pavlakis, Matthew Cooper
Summary: Well-selected patients with kidney disease and diabetes mellitus who undergo simultaneous kidney-pancreas transplantation often experience improved quality of life and long-term survival. Frailty has become increasingly important in the pancreas transplant candidate and recipient populations. This review discusses the risk factors, measurement, outcomes, and interventions related to frailty in pancreas transplant patients.
CLINICAL TRANSPLANTATION
(2023)
Editorial Material
Urology & Nephrology
Krista L. Lentine, Tarek Alhamad
CLINICAL JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2023)
Article
Urology & Nephrology
Yasar Caliskan, Benjamin E. Hippen, David A. Axelrod, Mark A. Schnitzler, Kennan Maher, Tarek Alhamad, Ngam N. Lam, Siddiq Anwar, Vivek Kute, Krista L. Lentine
Summary: There is variation in perspectives and implementation of COVID-19 vaccine mandate policies among international solid organ transplant centers. Centers with a vaccine mandate are more likely to extend the requirement to candidates' support persons, cohabitants, and living donors. Broader stakeholder engagement is needed to increase acceptance of pretransplant COVID-19 vaccination and protect the health of transplant patients.
Article
Urology & Nephrology
Neeraj Singh, Ruixin Li, Tarek Alhamad, Mark A. Schnitzler, Roslyn B. Mannon, Mona D. Doshi, Kenneth J. Woodside, Benjamin E. Hippen, Matthew Cooper, Jon Snyder, David A. Axelrod, Krista L. Lentine
Summary: The COVID-19 pandemic has had a significant impact on living donor kidney transplantation, especially for Black patients. Ongoing attention is necessary to address transplant disparities as the pandemic evolves and vaccine acceptance disparities persist.
Letter
Urology & Nephrology
Mohamed M. Ibrahim, Massini Merzkani, Haris Murad, Anupam Pande, Karen Flores, Erik R. Dubberke, Tarek Alhamad
AMERICAN JOURNAL OF NEPHROLOGY
(2022)