Article
Urology & Nephrology
Jialing Liu, Yi Li, Lingna Lyu, Liang Xiao, Aliza A. Memon, Xin Yu, Arvin Halim, Shivani Patel, Abdikheyre Osman, Wenqing Yin, Jie Jiang, Said Naini, Kenneth Lim, Aifeng Zhang, Jonathan D. Williams, Ruth Koester, Kevin Z. Qi, Quynh-Anh Fucci, Lai Ding, Steven Chang, Ankit Patel, Yutaro Mori, Advika Chaudhari, Aaron Bao, Jia Liu, Tzong-Shi Lu, Andrew Siedlecki
Summary: This study reveals the regulatory role of miR-218-5p in kidney endothelial progenitor cell migration, which may contribute to the development of therapeutic strategies for microvascular kidney injury.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2022)
Article
Urology & Nephrology
Takuto Chiba, Debora M. Cerqueira, Yao Li, Andrew J. Bodnar, Elina Mukherjee, Katherine Pfister, Yu Leng Phua, Kai Shaikh, Brandon T. Sanders, Shelby L. Hemker, Patrick J. Pagano, Yijen L. Wu, Jacqueline Ho, Sunder Sims-Lucas
Summary: miR-17 similar to miR-92 is dynamically regulated in renal endothelial cells after renal IRI and plays a role in the pathogenesis of AKI. Endothelial-specific miR-17 similar to miR-92 knockout exacerbates renal IRI, promoting renal tubular injury, reducing renal blood flow, and increasing oxidative stress in the kidneys. miR-17 similar to miR-92 is critical in the angiogenic response after renal IRI, and treatment with miR-18a and miR-19b mimics can mitigate the damage.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2021)
Article
Multidisciplinary Sciences
June Seok Heo, Sinyoung Kim
Summary: This study found that human adipose mesenchymal stem cell-derived exosomes have anti-inflammatory and pro-angiogenic effects. Exosomes inhibit inflammation by activating ROCK1 and PTEN expression, and promote angiogenesis by releasing miR-132 and miR-146a.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Haifei Chen, Weiling Cao, Jiao Chen, Danbo Liu, Lingyun Zhou, Fang Du, Feiqi Zhu
Summary: This study investigated the role and molecular mechanism of miRNAs in multiple myeloma, revealing that aberrant expression of miR-218 contributes to drug resistance in MM by targeting LRRC28, suggesting LRRC28 as a potential therapeutic target for MM.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Article
Immunology
Alissa K. Rutman, Sarita Negi, Nasim Saberi, Kashif Khan, Jean Tchervenkov, Steven Paraskevas
Summary: Delayed graft function (DGF) in kidney transplantation is associated with ischemic injury and carries long term functional and immunological risks. Kidney perfusate-derived extracellular vesicles (KP-EV) express donor-specific human leukocyte antigen and can modulate immune responses in transplant recipients. This discovery could lead to novel intervention strategies to improve graft function and survival.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Elizabeth Gould, Jun Hee Kim
Summary: Spiking OLs, characterized by expression of voltage-activated Na+ and K+ channels, are crucial for generating spikes in OLs and belong to a transitional stage during differentiation. The deletion of SCN2A gene disrupts the population of current-expressing OLs and eliminates spiking OLs, showing the essential role of SCN2A in spiking OLs. SCN2A deletion does not affect overall OL proliferation but hinders the maturation of a subset of OLs, indicating the involvement of Na(v)1.2 in the heterogeneity of OL lineage cells and their development.
Article
Cell Biology
Xiao Ke, Zhiyong Liao, Xinlin Luo, Jun-Qiu Chen, Ming Deng, Yiteng Huang, Zanxin Wang, Minxin Wei
Summary: This study demonstrated that ECFC-exosomes protect against vascular injury caused by atherosclerosis or PICA by delivering miR-21-5p, restoring autophagic flux, and inhibiting SIAP1L2 expression.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Chemistry, Multidisciplinary
Tian-tian Liu, Huan-huan Xu, Ze-juan Liu, He-ping Zhang, Hai-tao Zhou, Zhi-xiang Zhu, Zhi-qiang Wang, Jing-yi Xue, Qiang Li, Yi Ma, Hong-jie You, Da-li Luo
Summary: Endothelial dysfunction is a central characteristic of cardiovascular pathogenesis in diabetes mellitus, characterized by impaired eNOS and NO bioavailability. This study identified the role of CaM in the dysfunction of eNOS in diabetes. Downregulated CaM expression was found to be responsible for endothelial injury and impaired angiogenesis in diabetes. The findings provide a novel mechanism and target for protecting against diabetic endothelial injury.
ACTA PHARMACOLOGICA SINICA
(2023)
Article
Physiology
Anna E. Stanhewicz, Rowan L. Schlarmann, Kaila M. Brustkern, Diana I. Jalal
Summary: The study demonstrates that women who have had gestational diabetes during pregnancy are at higher risk for cardiovascular disease and type 2 diabetes in the decade following pregnancy. Microvascular dysfunction, mediated by increased oxidative stress, persists after pregnancy in women who had gestational diabetes.
JOURNAL OF APPLIED PHYSIOLOGY
(2022)
Article
Immunology
Ziang Zhu, Tong Li, Jinya Chen, Jai Kumar, Princy Kumar, Jing Qin, Colleen Hadigan, Irini Sereti, Jason V. Baker, Marta Catalfamo
Summary: This study examines the impact of HIV infection on endothelial inflammation and repair by evaluating cell phenotypes and subsets in the blood of HIV-infected individuals. The researchers found that infection-induced phenotypic changes were associated with inflammatory biomarkers, suggesting that HIV infection may affect endothelial inflammation and repair.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Liujun Jiang, Xiaolei Sun, Jiacheng Deng, Yanhua Hu, Qingbo Xu
Summary: Significant progress has been made in understanding the nature and functions of vascular stem cells, particularly in cardiovascular research. Recent studies using genetic lineage tracing mouse models have shed light on the behaviors of these cells during physiological angiogenesis and in vascular diseases. Further research using new techniques is crucial to fully elucidate the role of vascular stem cells in development and diseases.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Article
Immunology
Xiaoyu Pan, Lin Yang, Shuqi Wang, Yanhui Liu, Lin Yue, Shuchun Chen
Summary: The low-grade inflammatory state in obesity can damage vascular endothelial cells and lead to cardiovascular diseases. Macrophage exosomes improve glucose tolerance and insulin sensitivity in obese mice, but the relationship with endothelial cell injury is unclear. This study examined the effects of lipopolysaccharide (LPS)-induced macrophage exosomes on endothelial progenitor cells (EPCs) and inflammatory factors. It also investigated the role of MicroRNA-155 (miR-155) in macrophage exosomes and EPCs, and the impact of semaglutide on EPC function and inflammation. The results showed that LPS-induced macrophage exosomes impaired EPCs function and increased inflammation, which was exacerbated by high miR-155 expression. In contrast, inhibition of miR-155 improved EPCs function and reduced inflammation. Semaglutide enhanced EPCs viability and reduced inflammatory factors and miR-155 expression in exosomes.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Review
Pharmacology & Pharmacy
Yongpan Huang, Chong Song, Jianbin He, Min Li
Summary: Endothelial cells, as important metabolic and endocrine cells, play a crucial role in regulating vascular function. Various cardiovascular and cerebrovascular diseases are linked to endothelial dysfunction. The mechanisms of endothelial injury involve inflammation and oxidative stress, and endothelial progenitor cells are believed to contribute to the repair of damaged endothelium.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Urology & Nephrology
Melissa H. Little, Sara E. Howden, Kynan T. Lawlor, Jessica M. Vanslambrouck
Summary: Studying lineage relationships in cells can offer valuable insights into their origins, differentiation, and development. With the use of genetic methods, Cre systems, single-cell transcriptional profiling, and CRISPR-Cas9 gene editing, researchers are now able to make predictions about cellular relationships across different developmental stages, leading to a better understanding of development and disease.
NATURE REVIEWS NEPHROLOGY
(2022)
Article
Multidisciplinary Sciences
Chiu-Yang Lee, Shing-Jong Lin, Tao-Cheng Wu
Summary: This study investigated the regulation of miR-548j-5p in patients with peripheral artery disease (PAD) and its impact on angiogenesis of endothelial progenitor cells (EPCs). The results showed that up-regulation of miR-548j-5p promoted angiogenesis in ischemic tissue, suggesting it as a potential therapeutic approach for PAD.
SCIENTIFIC REPORTS
(2022)
Letter
Medicine, General & Internal
Ankit B. Patel, Ashish Verma, Gearoid M. McMahon
AMERICAN JOURNAL OF MEDICINE
(2021)
Article
Urology & Nephrology
Takuya Fujimaru, Kunio Kawanishi, Takayasu Mori, Eikan Mishima, Akinari Sekine, Motoko Chiga, Masayuki Mizui, Noriaki Sato, Motoko Yanagita, Yuki Ooki, Kiyotaka Nagahama, Yuko Ohnuki, Naoto Hamano, Saki Watanabe, Toshio Mochizuki, Katsushi Nagatsuji, Kenichi Tanaka, Tatsuo Tsukamoto, Hideo Tsushima, Mamiko Shimamoto, Takahiro Tsuji, Tamaki Kuyama, Shinya Kawamoto, Kenji Maki, Ai Katsuma, Mariko Oishi, Kouhei Yamamoto, Shintaro Mandai, Hiroaki Kikuchi, Fumiaki Ando, Yutaro Mori, Koichiro Susa, Soichiro Iimori, Shotaro Naito, Tatemitsu Rai, Junichi Hoshino, Yoshifumi Ubara, Mariko Miyazaki, Michio Nagata, Shinichi Uchida, Eisei Sohara
Summary: This study analyzed the genetic background and clinicopathologic features of adult NPH in 18 sporadic adult patients, finding that thick TBM duplication is a specific finding in adult NPH. Patients with pathogenic mutations were significantly younger, and older patients tended to have no pathogenic mutations even when suspected to have NPH by renal biopsy.
KIDNEY INTERNATIONAL REPORTS
(2021)
Article
Cell Biology
Yutaro Mori, Amrendra K. Ajay, Jae-Hyung Chang, Shan Mou, Huiping Zhao, Seiji Kishi, Jiahua Li, Craig R. Brooks, Sheng Xiao, Heung-Myong Woo, Venkata S. Sabbisetti, Suetonia C. Palmer, Pierre Galichon, Li Li, Joel M. Henderson, Vijay K. Kuchroo, Julie Hawkins, Takaharu Ichimura, Joseph Bonventre
Summary: Tubulointerstitial abnormalities can predict the progression of diabetic kidney disease, with KIM-1 playing a crucial role in this process. Targeting KIM-1 could be a potential therapeutic approach to alleviate renal damage.
Article
Biochemistry & Molecular Biology
Li Li, Pierre Galichon, Xiaoyan Xiao, Ana C. Figueroa-Ramirez, Diana Tamayo, Jake J-K Lee, Marian Kalocsay, David Gonzalez-Sanchez, Maria S. Chancay, Kyle W. McCracken, Nathan N. Lee, Takaharu Ichimura, Yutaro Mori, M. Todd Valerius, Julia Wilflingseder, Dario R. Lemos, Elazer R. Edelman, Joseph Bonventre
Summary: COUP-TFII plays a crucial role in fibrosis, with its overexpression suppressing fatty acid oxidation, enhancing glycolysis pathways, and inducing fibroblasts to produce collagen. Genetic ablation of COUP-TFII can mitigate kidney fibrosis after injury. The regulation of PGC1α by COUP-TFII serves as an important mechanism in fibrosis.
Article
Chemistry, Multidisciplinary
Christian Wiraja, Yutaro Mori, Takaharu Ichimura, Jangsun Hwang, Chenjie Xu, Joseph Bonventre
Summary: This study developed a human kidney tubuloid system combined with NF mRNA nanosensors for real-time evaluation of drug-induced nephrotoxicity. The research showed that NF nanosensors are specific and efficient in assessing tubular cell injury.
Article
Health Care Sciences & Services
Sonu Subudhi, Ashish Verma, Ankit B. Patel, C. Corey Hardin, Melin J. Khandekar, Hang Lee, Dustin McEvoy, Triantafyllos Stylianopoulos, Lance L. Munn, Sayon Dutta, Rakesh K. Jain
Summary: This study compared the performance of 18 machine learning algorithms in predicting ICU admission and mortality among COVID-19 patients, finding that ensemble-based models outperformed other model types in predicting both 5-day ICU admission and 28-day mortality from COVID-19. Implementing these models could aid in clinical decision-making for future infectious disease outbreaks.
NPJ DIGITAL MEDICINE
(2021)
Article
Urology & Nephrology
Jialing Liu, Yi Li, Lingna Lyu, Liang Xiao, Aliza A. Memon, Xin Yu, Arvin Halim, Shivani Patel, Abdikheyre Osman, Wenqing Yin, Jie Jiang, Said Naini, Kenneth Lim, Aifeng Zhang, Jonathan D. Williams, Ruth Koester, Kevin Z. Qi, Quynh-Anh Fucci, Lai Ding, Steven Chang, Ankit Patel, Yutaro Mori, Advika Chaudhari, Aaron Bao, Jia Liu, Tzong-Shi Lu, Andrew Siedlecki
Summary: This study reveals the regulatory role of miR-218-5p in kidney endothelial progenitor cell migration, which may contribute to the development of therapeutic strategies for microvascular kidney injury.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2022)
Article
Medicine, Research & Experimental
Yingke Zhou, Xin Jin, Haixin Yu, Gengdu Qin, Penglin Pan, Jingyuan Zhao, Taoyu Chen, Xueyi Liang, Yan Sun, Bo Wang, Dianyun Ren, Shikai Zhu, Heshui Wu
Summary: This study reveals the role of HDAC5 in regulating the NF-KB signaling pathway and antitumor immune responses in PDAC. HDAC5 downregulation is associated with favorable prognosis in PDAC patients. HDAC5 regulates PD-L1 expression by interacting with NF-KB p65 and its inhibition sensitizes PDAC tumors to immune checkpoint blockade therapy.
Article
Pathology
Saran Lotfollahzadeh, Dominic Lo, Emily A. York, Marc A. Napoleon, Wenqing Yin, Nagla Elzinad, John Le, Mengwei Zhang, Xiaosheng Yang, Austin Morrissey, Murad Elsadawi, Qing Zhao, Scott E. Schaus, Ulla Hansen, Vipul C. Chitalia
Summary: This study reveals the Wnt inhibitory and CRC growth-suppressive effects of LSF inhibitors in CRC cells, providing a novel target in CRC therapeutics.
AMERICAN JOURNAL OF PATHOLOGY
(2022)
Article
Cell Biology
Tomoki Yanagi, Hiroaki Kikuchi, Koichiro Susa, Naohiro Takahashi, Hiroki Bamba, Takefumi Suzuki, Yuta Nakano, Tamami Fujiki, Yutaro Mori, Fumiaki Ando, Shintaro Mandai, Takayasu Mori, Koh Takeuchi, Shinya Honda, Satoru Torii, Shigeomi Shimizu, Tatemitsu Rai, Shinichi Uchida, Eisei Sohara
Summary: This study presents ULK1 as a novel therapeutic target for CKD treatment that regulates AMPK activity in the kidney.
Article
Biotechnology & Applied Microbiology
Min Shi, Kyle W. McCracken, Ankit B. Patel, Weitao Zhang, Lioba Ester, M. Todd Valerius, Joseph Bonventre
Summary: This study describes a highly efficient method for differentiating human pluripotent stem cells into kidney collecting duct cells. The process involves inducing the stem cells into progenitor cells, forming organoids with similar morphology to fetal kidney, and ultimately differentiating into functional collecting duct cells.
NATURE BIOTECHNOLOGY
(2023)
Article
Cell Biology
Lang Jia, Yun Zhang, Feng Pu, Chong Yang, Shula Yang, Jinze Yu, Zihan Xu, Hongji Yang, Yu Zhou, Shikai Zhu
Summary: The amplification of AK4P1 targets YAP1 through competitive binding with miR-375, promoting the onset and development of PDAC.
Article
Medicine, Research & Experimental
Nkiruka Arinze, Wenqing Yin, Saran Lotfollahzadeh, Marc Arthur Napoleon, Sean Richards, Joshua A. Walker, Mostafa Belghasem, Jonathan D. Ravid, Mohamed Hassan Kamel, Stephen A. Whelan, Norman Lee, Jeffrey J. Siracuse, Stephan Anderson, Alik Farber, David Sherr, Jean Francis, Naomi M. Hamburg, Nader Rahimi, Vipul C. Chitalia
Summary: Chronic kidney disease (CKD) is an independent risk factor for peripheral artery disease (PAD). The role of uremic solutes retained in CKD patients in PAD is not well understood. This study reveals that tryptophan-derived uremic solutes inhibit beta-catenin, a key regulator of angiogenesis, in several cell types including endothelial cells. These solutes downregulate beta-catenin through the aryl hydrocarbon receptor (AHR). CKD mouse models show reduced beta-catenin and blood vessel density in ischemic limbs, which correlates with increased levels of uremic solutes and AHR activity. In a cohort of PAD patients, higher plasma levels of tryptophan metabolites and increased AHR-inducing activity in endothelial cells are associated with an increased risk of adverse limb events. Targeting the tryptophan metabolite/AHR/beta-catenin axis may provide new therapeutic strategies for PAD in CKD patients.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
