Article
Immunology
Fabian Pott, Dylan Postmus, Richard J. P. Brown, Emanuel Wyler, Elena Neumann, Markus Landthaler, Christine Goffinet
Summary: The study analyzed the cell-intrinsic responses of human synovial fibroblasts to CHIKV infection, finding that both baseline and exogenous type I interferon could partially restrict infection. RNA-seq revealed upregulation of gene expression induced by infection, while single-cell RNA-seq showed a fine-tuned switch from induction to repression of cell-intrinsic immune responses depending on viral RNA abundance in individual cells.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Cell Biology
Gal Avital, Felicia Kuperwaser, Andrew W. Pountain, Keenan A. Lacey, Erin E. Zwack, Magdalena Podkowik, Bo Shopsin, Victor J. Torres, Itai Yanai
Summary: This study investigates the dynamics of macrophage inflammation in response to various bacterial pathogens. Transcriptomic analysis reveals different cellular states within responding macrophages, and modulating the duration of infection and the presence of toxins impacts the trajectory of inflammation.
Article
Biochemistry & Molecular Biology
Shuoshuo Wang, Weihao Yan, Xiaoyu Yang, Jiayu Zhang, Qinghua Shi
Summary: This study integrated transcriptomic and methylomic analyses to explore the role of DNA methylation in modulating melon's transcriptional response to powdery mildew infection. The findings revealed that differential methylation-associated genes may play a crucial role in melon resistance to Podosphaera xanthii, providing potential targets for enhancing melon disease resistance in the future.
Article
Cell Biology
Pierre B. Cattenoz, Sara Monticelli, Alexia Pavlidaki, Angela Giangrande
Summary: Recent studies have expanded the catalog of Drosophila immune cells, revealing a greater molecular diversity than previously known. Comparative analyses have identified eight common subgroups associated with distinct functions, such as proliferation, immune response, phagocytosis, or secretion.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Barbara Lasut-Szyszka, Beata Malachowska, Agnieszka Gdowicz-Klosok, Malgorzata Krzesniak, Magdalena Glowala-Kosinska, Artur Zajkowicz, Marek Rusin
Summary: The combination of actinomycin D and nutlin-3a strongly activates p53, while CHIR-98014 prevents the activation of genes associated with Alzheimer's disease. RNA-Seq analysis identified novel p53-target genes and genes affected by CHIR-98014. The study suggests that an unidentified kinase inhibited by CHIR-98014 may play a role in modifying p53 to activate genes associated with innate immunity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Purva Singh, Samantha G. Lessard, Piali Mukherjee, Brennan Rourke, Miguel Otero
Summary: The phenotypic changes in articular chondrocytes during osteoarthritis are associated with alterations in DNA methylation patterns, which may contribute to cartilage destruction. By combining in vitro culture systems with RNA sequencing and enhanced bisulfite sequencing, researchers identified transcriptomic and epigenomic changes in hypertrophic differentiation of chondrocytes. The integrative analyses revealed associations between methylation changes and gene expression during hypertrophic differentiation, suggesting new therapeutic targets may be uncovered.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Ji Xiao, Ping Liu, Yiliang Wang, Yexuan Zhu, Qiongzhen Zeng, Xiao Hu, Zhe Ren, Yifei Wang
Summary: DNA methylation plays a significant role in innate immune responses and antiviral ability, and decitabine, as an efficient DNA methyltransferase inhibitor, has shown promising results. Understanding the regulation of decitabine could lead to the development of therapeutic methods for reducing morbidity and mortality associated with pathogen infections, particularly virus infections.
Article
Plant Sciences
Yaya Cui, Dongqin Chen, Yuexu Jiang, Dong Xu, Peter Balint-Kurti, Gary Stacey
Summary: The study investigated the differences in MAMP-triggered immune responses in sorghum and identified a core set of genes associated with the response. Samples from SC155-14E showed more differentially expressed genes related to immunity and pathogen resistance compared to BTx623.
Article
Biochemistry & Molecular Biology
Julia Panov, Hanoch Kaphzan
Summary: This study utilized a multi-omics approach to investigate the epigenomic and transcriptomic levels of human-derived neurons in Angelman syndrome (AS). The results showed that the genome of AS postmortem brain tissue had lower methylation levels and more upregulated genes compared to healthy controls. Many of these dysregulated genes are known to play critical roles in neuronal development and synaptic functioning, suggesting their involvement in AS development and its pathological features.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Matteo Vecellio, Elvezia Maria Paraboschi, Angela Ceribelli, Natasa Isailovic, Francesca Motta, Giulia Cardamone, Michela Robusto, Rosanna Asselta, Sonia Brescianini, Francesco Sacrini, Antonio Costanzo, Maria De Santis, Maria Antonietta Stazi, Stefano Duga, Carlo Selmi
Summary: A study on monozygotic twins revealed DNA methylation differences in psoriatic disease patients compared to controls, involving genes related to the TGF-beta signaling pathway. Transcriptome data further identified the IL-6/JAK/STAT3 and TNF-alpha pathways as key players in the disease.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Yuru Han, Baoyu Duan, Jing Wu, Yanjun Zheng, Yinchen Gu, Xiaomeng Cai, Changlian Lu, Xubo Wu, Yanfei Li, Xuefeng Gu
Summary: This study utilized RNA-seq and MeDIP-seq to analyze the genetic and epigenetic features of myocardial infarction (MI) at the early stages, revealing changes in gene expression and methylation regions associated with immune response, apoptosis, and energy metabolism. The study identified potential drug targets in genes related to apoptosis and inflammation, as well as highlighted the importance of methylation changes in genes affecting processes such as cardiac muscle contraction and angiogenesis during MI progression.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Rheumatology
Kleio-Maria Verrou, Nikolaos Vlachogiannis, Giannis Ampatziadis-Michailidis, Panagiotis Moulos, Georgios A. Pavlopoulos, Pantelis Hatzis, George Kollias, Petros P. Sfikakis
Summary: Transcriptome analysis in BD patients' blood mononuclear cells revealed significant upregulation and downregulation of genes, particularly related to neutrophil chemotaxis. This suggests a prominent enhancement of innate immune responses in BD, with a central role played by upregulated neutrophils.
Article
Environmental Sciences
Lulu Zheng, Yuwen Wang, Yule Zhang, Yongfeng Fu, Zhijin Yang, Yan Fan, Zhen Sun, Mantong Zhao, Lijun Zhu, Bo Dai, Dong An, Dawei Zhang, Sixiu Liu
Summary: This study utilized multi-omics analysis to investigate the impact of fine particles on lung cancer cells, revealing changes in gene methylation levels and activation of cellular calcium and apoptosis pathways after exposure to PM2.5.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Review
Oncology
Ali Mehdi, Shafaat A. Rabbani
Summary: Epigenetic mechanisms, particularly DNA and RNA methylation, play a crucial role in regulating immune responses, including in cancer and non-cancer contexts. Understanding the complex transcriptional regulation modulating differentiation and function of immune cells may lead to the identification of therapeutic targets aimed at targeting DNA and RNA methylation to reduce cancer-associated morbidity and mortality.
Article
Plant Sciences
Monica Canton, Cristian Forestan, Gianpiero Marconi, Esther Carrera, Claudio Bonghi, Serena Varotto
Summary: In temperate zones, fruit trees adjust their annual growth cycle to seasonal changes. Chilling accumulation allows peach flower bud development to be completed, accompanied by changes in transcription, epigenetic modifications, and chromatin structure.