Article
Biotechnology & Applied Microbiology
S. I. Ekonomou, F. F. Parlapani, M. Kyritsi, C. Hadjichristodoulou, I. S. Boziaris
Summary: This study monitored the storage of vacuum-packed hot smoked rainbow trout fillets from two different smokehouses in Greece at different temperatures and observed changes in microbiology. The shelf-life of the products differed between the two smokehouses. The study also identified and classified microbial communities grown at different pH levels.
Article
Chemistry, Analytical
Zhenzhen Han, Cheng Peng, Jia Yi, Dongxue Zhang, Xiaowei Xiang, Xinyan Peng, Bin Su, Baohong Liu, Yuhui Shen, Liang Qiao
Summary: A size-dependent microfluidic chip was developed to efficiently isolate and purify exosomes from human blood, showing high protein cleaning efficiency and exosome recovery rate compared to traditional ultracentrifugation. The method uses symmetric layers of PMMA with a nanoporous PCTE membrane, allowing for quick and automated large-cohort clinical usage.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Applied
Zhisong Wang, Yuxuan Zhao, Di Wang, Xianglong Zhang, Menglei Xia, Ting Xia, Yu Zheng, Min Wang
Summary: To investigate the formation of typical melanoidin polymers, methylglyoxal (MGO) was reacted with NH3 or alanine (Ala) to form coloured compounds. Control compounds were formed using glyoxal or acetone. The characterisation of the products was done using chromatography, mass spectrometry, and spectroscopy. The results showed that the coloured compounds formed were similar to melanoidins in food, and the MGO-based reaction generated similar volatile compounds using the Maillard reaction. The molecular weights of the structural units in the polymers were mainly 162, 169, and 176 Da, which could be reassembled using basic units derived from MGO or in combination with nitrogen. Therefore, polymers recombined using basic structural units should be considered when determining melanoidin biomarkers. The preparation of coloured compounds using MGO with NH3 can be used as a novel method for producing control compounds for melanoidin after process optimization.
Review
Microbiology
Fernando Sanchez-Juanes, Noelia Calvo Sanchez, Moncef Belhassen Garcia, Carmen Vieira Lista, Raul Manzano Roman, Rufino Alamo Sanz, Antonio Muro Alvarez, Juan Luis Munoz Bellido
Summary: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a simple, fast, and specific method used for bacterial identification, and has also been shown to be useful for identifying certain parasites and arthropod vectors. Fast, simple, and reliable methods for identifying arthropod vectors are crucial for controlling diseases transmitted by these vectors.
Article
Food Science & Technology
Khemmapas Treesuwan, Wannee Jirapakkul, Vanee Chonhenchob, Sasitorn Tongchitpakdee, Warapa Mahakarnchanakul, Kullanart Tongkhao
Summary: This study developed a sulfite-free treatment combined with modified atmosphere packaging (MAP) to prolong the shelf life of trimmed young coconuts (TYC) during cold storage. The research showed that this treatment effectively controlled the qualities of TYC over an 8-week storage period compared to the conventional sulfite treatment.
Review
Chemistry, Analytical
Hugo M. Santos, Luis B. Carvalho, Carlos Lodeiro, Goncalo Martins, Ines L. Gomes, Wilson D. T. Antunes, Vanessa Correia, Maria M. Almeida-Santos, Helena Rebelo-de-Andrade, Antonio P. A. Matos, J. L. Capelo
Summary: This work presents a tutorial on the capabilities of bioanalytical mass spectrometry in detecting and differentiating viruses at the peptide level, as well as providing diagnosis and prognosis for infected patients. It offers insights into the possibilities of current high-resolution mass spectrometry technology and bioinformatics.
MICROCHEMICAL JOURNAL
(2023)
Article
Microbiology
Eloise J. Busby, Ronan M. M. Doyle, Clara Leboreiro Babe, Kathryn A. A. Harris, Damien Mack, Gema Mendez-Cervantes, Denise M. M. O'Sullivan, Vicky Pang, Zahra Sadouki, Priya Solanki, Jim F. F. Huggett, Timothy D. D. McHugh, Emmanuel Q. Q. Wey
Summary: Managing the transmission of antimicrobial resistance requires reliable methods for tracking outbreaks. MALDI-TOF MS was compared with orthogonal approaches for strain typing, including WGS and FTIR, for Acinetobacter baumannii isolates associated with a healthcare-associated infection event. Reliable molecular typing methods can help to trace transmission routes and manage outbreaks.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Liangqiang Chen, Wenjing Gao, Xue Tan, Ying Han, Fu Jiao, Bin Feng, Jinghang Xie, Bin Li, Huilin Zhao, Huabin Tu, Shaoning Yu, Li Wang
Summary: MALDI-TOF MS is a method that can be used to classify specific model microbiota with high accuracy. The actual MS spectrum of the model microbiota shows a specific spectral fingerprint, which enhances the specificity and accuracy of microbiota classification.
MICROBIOLOGY SPECTRUM
(2023)
Article
Agricultural Engineering
Juana Rozalen, M. Mercedes Garcia, Amaya Zalacain, Horacio Lopez-Corcoles, Jorge Hurtado de Mendoza, Katrina Cornish, Manuel Carmona
Summary: Guayule, as a promising renewable source of natural rubber/latex, has resins with various activities potentially related to sesquiterpenes. By exploring different analytical methods, it was found that there may be new members of the guayulins family and the relationship in seasonal changes was identified.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Infectious Diseases
Natalia Kehl Moreira, Camila Morschbacher Wilhelm, Ayme Duarte Echevarria, Fabiana Caroline Zempulski Volpato, Priscila Lamb Wink, Afonso Luis Barth, Juliana Caierao
Summary: Detection of KPC enzyme directly from positive blood cultures using MALDI-TOF MS is feasible and has high sensitivity and specificity. This method can rapidly and accurately assess bacterial resistance mechanisms, which is crucial for selecting appropriate treatment and improving antimicrobial stewardship.
Review
Environmental Sciences
Mohammad Y. Ashfaq, Dana A. Da'na, Mohammad A. Al-Ghouti
Summary: Bacteria play diverse roles in the environment, with MALDI-TOF MS technology gaining attention for routine bacterial identification. This technique has been applied in various environmental studies, showing benefits in rapid and cost-effective bacteria identification.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Microbiology
Adriana Calderaro, Mirko Buttrini, Benedetta Farina, Sara Montecchini, Monica Martinelli, Maria Cristina Arcangeletti, Carlo Chezzi, Flora De Conto
Summary: The epidemiology of Clostridioides difficile infection (CDI) has changed over the past two decades, with the emergence of clinically relevant C. difficile strains causing severe nosocomial outbreaks. This study reports on an outbreak that occurred in a Long-term Care Unit, which was tracked using a Matrix-Assisted Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) typing approach (T-MALDI). The study also characterized the toxigenic and antimicrobial susceptibility profiles of the C. difficile isolates. The results showed the presence of three different clusters, with one cluster being considered epidemic and susceptible to vancomycin and metronidazole but resistant to moxifloxacin. This is the first reported outbreak associated with a tcdA+/tcdB-/cdt+ genotypic profile. T-MALDI is shown to be a valid and rapid approach for detecting and tracking outbreaks.
Article
Biochemistry & Molecular Biology
Chia-Ru Chung, Hsin-Yao Wang, Po-Han Chou, Li-Ching Wu, Jang-Jih Lu, Jorng-Tzong Horng, Tzong-Yi Lee
Summary: This study used an ensemble of multiple preprocessing methods to extract critical information from complicated mass spectrometry spectral data for identifying microorganisms and predicting antibiotic resistance. The ensemble method outperformed individual methods, achieving the highest accuracy on independent testing datasets. Important peaks related to antibiotic resistance could be detected, providing valuable information for further investigation of the resistance mechanism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Claudia Honsig, Brigitte Selitsch, Marlene Hollenstein, Matthias G. Vossen, Kathrin Spettel, Birgit Willinger
Summary: In this study, the identification of 230 filamentous fungi isolates using MALDI-TOF MS in combination with the Filamentous Fungi Library 3.0 was investigated. The results showed that using the more time-consuming sample preparation methods improved species-level identification rates, and the in-house threshold for species identification was validated for routine use in the laboratory. This suggests that this commercial platform is suitable for routine diagnosis.
Article
Chemistry, Organic
Bernard Fernandez, Jean Armengaud, Gilles Subra, Christine Enjalbal
Summary: This study investigates the feasibility of using high energy charge remote fragmentations to analyze the N-terminus of proteins, and compares it with low energy collision-induced dissociations. The results show that the amino acid composition significantly affects the dissociation pathways of peptide backbones.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Engineering, Biomedical
Mohsen Janmaleki, Jun Liu, Milad Kamkar, Milad Azarmanesh, Uttandaraman Sundararaj, Amir Sanati Nezhad
Summary: This study investigates the effect of thermoreversible physical crosslinking on the printability of GelMA, revealing that decreasing the extrusion temperature improves filament formation and shape fidelity of the deposited hydrogel. Mechanical testing and rheological studies confirm that lowering the extrusion temperature enhances the elastic properties of the extruded filaments. By utilizing various analysis methods, it is shown that the extruded samples printed at lower temperatures exhibit higher solid behavior and reduced porosity, with changes in linear and non-linear viscoelastic properties.
BIOMEDICAL MATERIALS
(2021)
Article
Engineering, Biomedical
Kar Wey Yong, Mohsen Janmaleki, Mohammad Pachenari, Alim P. Mitha, Amir Sanati-Nezhad, Arindom Sen
Summary: Physiologically relevant intracranial aneurysm (IA) models are crucial for testing treatment options for IA. In this study, a new in vitro tissue-engineered platform was developed to mimic the microenvironment, structure, and cellular complexity of native human IA. The cellularized IA models demonstrated high viability and maintained their secretome profiles during medium perfusion, showing the biocompatibility of the hydrogel. The IA models showed the ability to predict the response of IA to different fluid flow profiles, providing insights into the pathophysiology and treatment options for IA.
ACTA BIOMATERIALIA
(2021)
Article
Biophysics
Mehdi Mohammadi, Hossein Zargartalebi, Razieh Salahandish, Raied Aburashed, Kar Wey Yong, Amir Sanati-Nezhad
Summary: This study provides an overview of the role of exosomes in cancer diagnosis and prognosis, investigates emerging exosome technologies, evaluates the advantages and drawbacks of different technologies, and discusses their clinical challenges for cancer diagnosis and prognosis. Commercial platforms for exosomal detection and analysis are introduced, with a focus on their performance and impact on cancer diagnosis and prognosis, as well as the risks associated with the development of next-generation exosome devices.
BIOSENSORS & BIOELECTRONICS
(2021)
Editorial Material
Engineering, Chemical
Anil K. Mehrotra, Kunal Karan, Michael S. Kallos, Arin Sen
CANADIAN JOURNAL OF CHEMICAL ENGINEERING
(2021)
Review
Chemistry, Multidisciplinary
Sara Hassanpour Tamrin, Amir Sanati Nezhad, Arindom Sen
Summary: Exosomes are cell-derived structures containing lipids, proteins, and nucleic acids, playing roles in physiological and pathological processes. Microfluidic technology offers simple, cost-effective, precise, and fast processing for exosome isolation, yet faces challenges hindering their clinical utility.
Article
Cell Biology
Mary Crisol, Kar Wey Yong, Kezhou Wu, Leila Laouar, Janet A. W. Elliott, Nadr M. Jomha
Summary: Both clinical-grade and research-grade additives significantly improved cell viability compared with the negative control. CS, AA, and NAC+AA also increased cell viability significantly compared with the negative control. However, NAC, NAC+CS, and CS+AA did not show improvement in cell viability compared with the negative control. Supplementation with clinical-grade CS or AA significantly improved chondrocyte viability in porcine cartilage at high CPA concentrations, while supplementation with clinical-grade NAC did not have a beneficial effect. Supplementing with clinical-grade additives in CPA solutions can mitigate CPA toxicity and is crucial for developing effective vitrification protocols for human tissue banks.
BIOPRESERVATION AND BIOBANKING
(2022)
Editorial Material
Cell & Tissue Engineering
Jane Ru Choi, Kar Wey Yong, Hui Yin Nam
STEM CELLS INTERNATIONAL
(2022)
Review
Oncology
Jane Ru Choi, Gul Kozalak, Ighli di Bari, Quratulain Babar, Zahra Niknam, Yousef Rasmi, Kar Wey Yong
Summary: Cancer, a leading cause of death worldwide, has limited clinical treatment options despite extensive research. Animal cancer models face several challenges, prompting the development of lab-grown human cancer models for biomedical applications. This review highlights the advantages, limitations, and applications of these models, including cancer therapeutic development and the discovery of key cancer markers.
Article
Chemistry, Physical
Hamideh Eskandari, Vinayaraj Ozhukil Kollath, Kunal Karan
Summary: The replacement of pure Pt catalysts with Pt-Co and Pt-Ni alloy catalysts has reduced the cost of fuel cells but also introduced performance degradation due to leaching out of cobalt and nickel. This study investigates the impact of proton-cobalt ion exchange on the hydration and conductivity of thin ionomer films, and finds that the water content decreases upon cobalt ion exchange, leading to a significant reduction in ionic conductivity.
JOURNAL OF PHYSICAL CHEMISTRY C
(2022)
Article
Multidisciplinary Sciences
Muhammad Naoshad Islam, Abdul Bashith Mansoor Basha, Vinayaraj Ozhukil Kollath, Amir Peyman Soleymani, Jasna Jankovic, Kunal Karan
Summary: This study demonstrates the successful development of low-Platinum content polymer electrolyte fuel cells with high oxygen reduction reaction (ORR) activity and fast oxygen transport by introducing a bottom-up designed spherical carbon support with intrinsic Nitrogen-doping.
NATURE COMMUNICATIONS
(2022)
Article
Materials Science, Biomaterials
Sultan Khetani, Razieh Salahandish, Jason B. Tabor, Matthew Chilvers, Sean Dukelow, Chester Ho, Christina Campbell, Arindom Sen, Chantel T. Debert, Amir Sanati-Nezhad
Summary: This research developed a novel nanoporous carbon screen-printed electrode to create an ultrasensitive GFAP immunosensor for rapid and accurate detection of GFAP levels in CNS injury patients' serum. The immunosensor showed comparable diagnostic and prognostic performance to existing clinical modalities, presenting the potential for rapid and accurate POC diagnosis and prognosis of CNS injuries in a cost-effective and near real-time manner.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Cell & Tissue Engineering
Jolene Phelps, David A. Hart, Alim P. Mitha, Neil A. Duncan, Arindom Sen
Summary: After ischemic brain injury, the induction of angiogenesis is crucial for neurological recovery. The angiogenic effects of mesenchymal stem cells (MSCs) have been attributed to the secretion of extracellular vesicles (EVs). EVs contain angiogenic biomolecules that can cross the blood-brain barrier (BBB) and elicit therapeutic responses. Culturing MSCs under physiologically relevant oxygen conditions has been found to increase the secretion of survival and angiogenic factors. This study investigates the effects of MSC-EVs on cerebral angiogenesis and whether MSCs cultured under physiological oxygen conditions provide greater functional effects.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell Biology
Sara Hassanpour Tamrin, Jolene Phelps, Amir Sanati Nezhad, Arindom Sen
Summary: Small extracellular vesicles (EVs) have significant roles in physiological and pathological processes, but uncertainties about their nature have complicated the development of EV-based diagnostics and therapeutics. Current methods for evaluating EV charge tend to underestimate it at the nanoscale and cannot fully account for tightly bound ions on the surface of these particles. This study introduces a new analytical method that accurately determines the charge of small EVs, providing valuable insights for the development of EV-based applications.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Meeting Abstract
Cell & Tissue Engineering
M. O. H. S. E. N. JANMALEK, Milad Kamkar, Milad Azarmanesh, Uttandaraman Sundararaj, Amir Sanati Nezhad, Jun Liu
TISSUE ENGINEERING PART A
(2022)
Article
Cell & Tissue Engineering
Jolene Phelps, Catherine Leonard, Sophia Shah, Roman Krawetz, David A. Hart, Neil A. Duncan, Arindom Sen
Summary: Mesenchymal progenitor cells (MPCs) have shown promise in initiating articular cartilage repair, largely due to the trophic factors they secrete. This study found that these factors can be found in the conditioned medium (CM) collected from cell cultures, with extracellular vesicles (EVs) believed to play a role in MPC therapeutic efficacy. The study also found that clinically relevant quantities of MPC-derived EVs can be generated using stirred suspension bioreactors (SSBs), which could potentially induce cartilage repair.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)