Article
Cell & Tissue Engineering
Alan F. Zahorchak, Misty L. DeRiggi, Jennifer L. Muzzio, Veronica Sutherland, Abhinav Humar, Fadi G. Lakkis, Yen-Michael S. Hsu, Angus W. Thomson
Summary: Regulatory dendritic cells (DCregs) have potential as a cell therapy for immune-mediated disorders. This study generated and validated large numbers of DCregs for infusion into liver transplant recipients. The DCregs met quality criteria and achieved target cell numbers, offering a promising approach to improve patient outcomes.
Article
Biochemistry & Molecular Biology
Katia Mareschi, Elena Marini, Alessia Giovanna Santa Banche Niclot, Marta Barone, Giuseppe Pinnetta, Aloe Adamini, Manuela Spadea, Luciana Labanca, Graziella Lucania, Ivana Ferrero, Franca Fagioli
Summary: This study demonstrates that the use of HPL-S is an effective alternative for MSC production under GMP conditions. The protein and growth factor content in HPL-S is comparable to that of HPL-E. Furthermore, MSCs derived from HPL-S show lower HLA-DR expression in immunophenotypic analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Gilberto Jaramillo-Rangel, Maria-de-Lourdes Chavez-Briones, Adriana Ancer-Arellano, Marta Ortega-Martinez
Summary: Nestin expression in lung cancer leads to an aggressive phenotype and resistance to chemotherapy and radiation treatments, while also potentially being involved in the pathogenesis of some non-cancer-related lung diseases. Additionally, nestin-positive cells may play a role in lung homeostasis and be capable of generating various types of lung tissues.
Article
Biochemistry & Molecular Biology
Stefano Canosa, Katia Mareschi, Elena Marini, Andrea Roberto Carosso, Sara Castiglia, Deborah Rustichelli, Ivana Ferrero, Gianluca Gennarelli, Benedetta Bussolati, Alberto Nocifora, Valentina Asnaghi, Massimiliano Bergallo, Ciro Isidoro, Chiara Benedetto, Alberto Revelli, Franca Fagioli
Summary: The study compared two different endometrial sampling methods and validated a novel xeno-free method for culturing human E-MSCs. Results showed that VABRA endometrial sampling could be an alternative to surgical curettage, providing efficient isolation and culture of E-MSCs in Good Manufacturing Practice compliance conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Chenghai Li, Hua Zhao, Linna Cheng, Bin Wang
Summary: MSC-based therapeutics are available for a range of diseases, with autologous and allogeneic MSCs having their own advantages and disadvantages in medical practice. The interaction of transplanted MSCs with their microenvironment can be impacted by factors from different donors, potentially leading to uncertainty in safety and efficacy. Donor-controlled clinical practice is suggested to minimize potential harm in MSC-based therapies.
CELL AND BIOSCIENCE
(2021)
Article
Chemistry, Analytical
Anthony T. Giduthuri, Sophia K. Theodossiou, Nathan R. Schiele, Soumya K. Srivastava
Summary: This study explores a method for identifying and separating tenogenically differentiated stem cells. Experimental results show that changes in dielectric properties potentially indicative of early tenogenesis can be detected as early as three days into differentiation. This method has the potential to address challenges in stem cell therapy for tendon injuries.
Article
Oncology
P. K. J. D. de Jonge, P. M. M. van Hauten, L. D. Janssen, A. L. de Goede, M. M. Berrien-Elliott, J. M. R. van der Meer, C. M. Mousset, M. W. H. Roeven, M. Foster, N. Blijlevens, W. Hobo, T. A. Fehniger, J. H. Jansen, N. P. M. Schaap, H. Dolstra
Summary: Allogeneic natural killer (NK) cell-based immunotherapy is a promising approach for acute myeloid leukemia (AML). A good manufacturing practice (GMP)-compliant, cytokine-based ex vivo manufacturing process for generating NK cells from CD34(+) hematopoietic stem and progenitor cells (HSPC) has been developed. This process generates a consistent and active NK cell product that meets the requirements for NK cell immunotherapy. The manufactured NK cell batches are used in a clinical trial approved by the Dutch Ethics Committee.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2023)
Article
Cell & Tissue Engineering
Andreas Kerstan, Elke Niebergall-Roth, Jasmina Esterlechner, Hannes M. Schroeder, Martin Gasser, Ana M. Waaga-Gasser, Matthias Goebeler, Katrin Rak, Philipp Schruefer, Sabrina Endres, Petra Hagenbusch, Korinna Kraft, Kathrin Dieter, Seda Ballikaya, Nicole Stemler, Samar Sadeghi, Nils Tappenbeck, George F. Murphy, Dennis P. Orgill, Natasha Y. Frank, Christoph Ganss, Karin Scharffetter-Kochanek, Markus H. Frank, Mark A. Kluth
Summary: The study demonstrates the potential of a novel subpopulation of mesenchymal stromal cells called ABCB5(+) MSCs manufactured as ATMPs through a GMP-compliant process, to provide a clinically relevant wound closure strategy for patients with chronic therapy-resistant wounds. The treatment showed a median wound size reduction of 63% at 12 weeks and early relief of pain in patients with therapy-resistant chronic venous ulcers. The data presented contribute to substantiating the idea of using ABCB5(+) MSCs as an effective treatment option for chronic therapy-resistant wounds.
Article
Biochemistry & Molecular Biology
Julia I. Khorolskaya, Daria A. Perepletchikova, Daniel V. Kachkin, Kirill E. Zhurenkov, Elga I. Alexander-Sinkler, Julia S. Ivanova, Natalia A. Mikhailova, Miralda I. Blinova
Summary: The study involved obtaining and characterizing rabbit L-MSCs, modifying them to express EGFP, and demonstrating their potential use for studying regenerative processes during the treatment of corneal pathologies.
Article
Biochemistry & Molecular Biology
Marco Miceli, Giuseppe Maria Maruotti, Laura Sarno, Luigi Carbone, Maurizio Guida, Alessandra Pelagalli
Summary: Regenerative medicine is a growing field in biomedical sciences that aims to develop new therapeutic strategies for repairing or regenerating damaged tissues and organs. Human mesenchymal stem cells (hMSCs) play a crucial role in tissue regeneration as they have the ability to differentiate into various cell types, including chondrocytes. Researchers have been focusing on optimizing hMSC differentiation protocols through in vitro strategies, including epigenetic manipulations. This study investigated the effects of the class II histone deacetylase (HDAC) inhibitor, MC1568, on chondrogenesis derived from hMSCs. Preliminary data showed that MC1568 improved and accelerated the chondrogenic differentiation of hMSCs in vitro.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell & Tissue Engineering
Kangkang Zha, Xu Li, Zhen Yang, Guangzhao Tian, Zhiqiang Sun, Xiang Sui, Yongjing Dai, Shuyun Liu, Quanyi Guo
Summary: Articular cartilage is difficult to repair due to its avascular nature, but mesenchymal stem cells (MSCs) show promise in cartilage regeneration. However, the heterogeneous nature of MSCs can lead to unstable therapeutic effects. Understanding MSC heterogeneity and utilizing different types of MSCs for cartilage regeneration can improve treatment efficiency.
NPJ REGENERATIVE MEDICINE
(2021)
Article
Cell & Tissue Engineering
Clarissa Lenero, Annie C. Bowles, Diego Correa, Dimitrios Kouroupis
Summary: This study investigated the molecular responses of human endometrial-derived mesenchymal stem/stromal cells (eMSCs) to different culture passages and inflammatory environments, revealing that the response of eMSCs to inflammation significantly affected their functional attributes.
Article
Engineering, Biomedical
Lihui Peng, Julien E. Gautrot
Summary: This study demonstrates that bioemulsions formed of microdroplets stabilized by protein nanosheets are well-suited for scale up of adherent stem cells like MSCs. Phenotyping of MSCs cultured on these bioemulsions, solid microcarriers, and classic 2D tissue culture polystyrene over multiple passages shows comparable phenotypes, although stemness is lost at late passages. This validates the use of bioemulsions for long term expansion of adherent stem cells and opens possibilities for novel 3D bioreactors based on microdroplet microcarriers.
MATERIALS TODAY BIO
(2021)
Retraction
Biochemistry & Molecular Biology
Sabine Wislet-Gendebien, Emerence Laudet, Virginie Neirinckx, Philippe Alix, Pierre Leprince, Aneta Glejzer, Christophe Poulet, Benoit Hennuy, Lukas Sommer, Olga Shakhova, Bernard Rogister
Summary: A correction to this paper has been published.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Cell Biology
Jeevithan Elango, Rodion Bushin, Artiom Lijnev, Piedad N. De Aza, Carlos Perez-Albacete Martinez, Jose Manuel Granero Marin, Ana Belen Hernandez, Luis Ramon Meseguer Olmo, Jose Eduardo Mate Sanchez De Val
Summary: Hydroxyapatite (HA) is a mineral component used for repair and regeneration of mineralized tissues. The addition of germanium (Ge) improves the properties of HA and shows biocompatibility with BM-MSCs.
Article
Pharmacology & Pharmacy
Jose L. Soriano, Ana C. Calpena, Maria J. Rodriguez-Lagunas, Oscar Domenech, Nuria Bozal-de Febrer, Maria L. Garduno-Ramirez, Beatriz Clares
Summary: This study investigated the efficacy of an antioxidant cocktail in a thermosensitive hydrogel delivery system for skin wound healing. In vivo models and atomic force microscopy were used to evaluate the healing effects, demonstrating that the antioxidant cocktail can promote wound healing with strong antioxidant and antimicrobial activity.
Article
Biotechnology & Applied Microbiology
Mireia Mallandrich, Ana C. Calpena, Beatriz Clares, Alexander Parra, Maria L. Garcia, Jose L. Soriano, Francisco Fernandez-Campos
Summary: The optimized KT-NPs with small particle size and high encapsulation efficiency showed superior release and retention in the eye compared to the commercial reference, without causing eye irritation. Therefore, KT-NPs are a suitable alternative for the treatment of eye inflammatory diseases.
Article
Biotechnology & Applied Microbiology
Maria Luisa Antequera-Gomez, Luis Diaz-Martinez, Juan Antonio Guadix, Ana Maria Sanchez-Tevar, Sara Sopena-Torres, Jesus Hierrezuelo, Hung K. Doan, Johan H. J. Leveau, Antonio deVicente, Diego Romero
Summary: Bacillus cereus is a common food-borne pathogen that can cause food poisoning in humans, with symptoms of vomiting or diarrhea. The study found that sporulation is necessary for the survival of B. cereus in leaves, but not essential in ready-to-eat vegetables. It also highlighted the underestimated number of food poisoning cases associated with B. cereus and the need to revise detection protocols based on spores and toxins.
MICROBIAL BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Paul Palmquist-Gomes, Jose Maria Perez-Pomares, Juan Antonio Guadix
Summary: A variety of experimental model organisms are used in developmental biology practical lectures to make students aware of the biological phenomena underlying normal animal embryogenesis. The design of practical laboratory lectures is crucial to foster students' interests in the subject.
BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION
(2021)
Review
Chemistry, Multidisciplinary
Eliana B. Souto, Eliezer Jaeger, Alessandro Jaeger, Petr Stepanek, Amanda Cano, Cesar Viseras, Raquel de Melo Barbosa, Marlus Chorilli, Aleksandra Zielinska, Patricia Severino, Beatriz C. Naveros
Summary: Despite the health benefits of the sun, overexposure without proper precautions can cause irreversible damage to the skin. To balance the risks and benefits, a technological alternative using lipid nanoparticulate systems as photoprotective agents has been found, which offers several advantages compared to chemical and physical sunscreens.
Article
Health Care Sciences & Services
Marcelo C. Ribeiro, Jose M. Rivera-Arbelaez, Carla Cofino-Fabres, Verena Schwach, Rolf H. Slaats, Simone A. ten Den, Kim Vermeul, Albert van den Berg, Jose M. Perez-Pomares, Loes I. Segerink, Juan A. Guadix, Robert Passier
Summary: This study developed a platform for generating three-dimensional engineered heart tissues (EHTs) from human pluripotent stem cells (hPSC-CMs), which can be used for studying heart disease and drug screening. The EHT platform demonstrated improved functional performance and histology when cultured in a serum-free maturation medium.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Paul Palmquist-Gomes, Ernesto Marin-Sedeno, Adrian Ruiz-Villalba, Gustavo Adolfo Rico-Llanos, Jose Maria Perez-Pomares, Juan Antonio Guadix
Summary: The presence of cartilage tissue in the hearts of vertebrate species has long been recognized. Recent findings suggest that epicardial lineage cells can differentiate into chondrocytes, challenging the historical view that the embryonic neural crest is the main source of cardiac cartilage. This study demonstrates the formation of cardiac cartilage clusters from proepicardial cells, supporting the concept of proepicardial cells as multipotent connective progenitors. These findings have important implications for understanding cardiac cell complexity and responses to pathological stimuli.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Lidia Gomez-Segura, Antoni Boix-Montanes, Mireia Mallandrich, Alexander Parra-Coca, Jose L. Soriano-Ruiz, Ana Cristina Calpena, Alvaro Gimeno, David Bellido, Helena Colom
Summary: Carprofen is a commonly used non-steroidal anti-inflammatory drug for treating respiratory diseases in animals. Its pharmacokinetics and bioavailability in pigs are not well-studied. However, a population pharmacokinetic analysis showed that carprofen is well-absorbed and has a similar distribution pattern in pigs compared to other mammals. The clearance and half-life of carprofen in pigs are slower than in humans and other animal species. Liver and kidney concentrations of carprofen in pigs were lower than those in the plasma. Simulations suggested that a dosage regimen of 4 mg/kg every 24 hours would achieve therapeutic levels in pigs similar to those observed in other animal species.
Article
Pharmacology & Pharmacy
Nuria Garros, Mireia Mallandrich, Negar Beirampour, Roya Mohammadi, Oscar Domenech, Maria Jose Rodriguez-Lagunas, Beatriz Clares, Helena Colom
Summary: The study proposes ocular liposomal formulations loaded with baricitinib for the management of Sjogren's syndrome. The liposomal formulations showed suitable physicochemical properties for ocular administration and did not have any irritant effects on ocular tissues. The study also found that L-alpha-PC led to better permeation in the sclera, while POPE:POPG led to better permeation in the cornea.
Article
Polymer Science
Noelia Perez-Gonzalez, Maria J. Rodriguez-Lagunas, Ana C. Calpena-Campmany, Nuria Bozal-de Febrer, Lyda Halbaut-Bellowa, Mireia Mallandrich, Beatriz Clares-Naveros
Summary: Fungal keratitis is a global cause of corneal blindness. Current treatment involves the use of antibiotics, with Natamycin being commonly used; however, alternative therapies are necessary due to the difficulty in treating fungal keratitis. In situ gelling formulations, which combine the advantages of eye drops and ointments, show promise as an alternative. This study aimed to develop and characterize three formulations containing 0.5% CSP: CSP-O1, CSP-O2, and CSP-O3. The results suggest that these formulations could be potential candidates for the treatment of fungal keratitis.
Article
Polymer Science
Noelia Perez-Gonzalez, Lupe Carolina Espinoza, Maria Rincon, Lilian Sosa, Mireia Mallandrich, Joaquim Suner-Carbo, Nuria Bozal-de Febrer, Ana Cristina Calpena, Beatriz Clares-Naveros
Summary: Caspofungin gel, incorporating Azone, showed higher release and retention of the drug in the skin compared to a promoter-free Caspofungin gel. Both formulations exhibited good tolerability and inhibited the growth of certain Candida species. Dermal treatment with Caspofungin could be a promising therapy for cutaneous candidiasis in refractory or intolerant patients.
Article
Pharmacology & Pharmacy
Cristina Galocha-Leon, Cristina Antich, Ana Voltes-Martinez, Juan A. Marchal, Mireia Mallandrich, Lyda Halbaut, Maria J. Rodriguez-Lagunas, Eliana B. Souto, Beatriz Clares-Naveros, Patricia Galvez-Martin
Summary: This study developed a hydrogel loaded with human mesenchymal stromal cells (hMSCs) for the treatment of superficial skin injuries. The hydrogel, using poloxamer 407 as a biomaterial, showed excellent physical, mechanical, morphological, and biological properties. It demonstrated high swelling capacity and did not affect the viability and healing capacity of keratinocytes.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Review
Microbiology
Patricia Severino, Wanessa Santana, Erika S. Lisboa, Victoria L. S. dos Santos, Erica T. dos Santos Lima, Juliana C. Cardoso, Ricardo L. C. de Albuquerque-Junior, Beatriz C. Naveros, Antonello Santini, Eliana B. Souto, Sona Jain
Summary: This article discusses the advantages and limitations of using topical drug delivery for the treatment of cutaneous leishmaniasis (CL) and mucocutaneous leishmaniasis (ML), as well as the relevance of combining this approach with other pharmaceutical dosage forms. The article highlights that topical treatment can improve patient compliance and overcome certain issues with currently available alternatives. The use of nanomaterials for site-specific delivery is also emphasized.
MICROBIOLOGY RESEARCH
(2022)
Article
Polymer Science
Noelia Perez-Gonzalez, Nuria Bozal-de Febrer, Ana C. Calpena-Campmany, Anna Nardi-Ricart, Maria J. Rodriguez-Lagunas, Jose A. Morales-Molina, Jose L. Soriano-Ruiz, Francisco Fernandez-Campos, Beatriz Clares-Naveros
Summary: The study developed and characterized three hydrogels containing chitosan, Poloxamer 407, and a combination of both with 2% caspofungin for the treatment of VVC. The formulations showed promising potential for treating VVC, with physically, mechanically, and morphologically characterized properties.
Article
Materials Science, Multidisciplinary
Sergio Gamez-Valenzuela, David Neusser, Carlos Benitez-Martin, Francisco Najera, Juan A. Guadix, Carlos Moreno-Yruela, Belen Villacampa, Rocio Ponce Ortiz, Sabine Ludwigs, Raquel Andreu, M. Carmen Ruiz Delgado
Summary: The synthesis and comprehensive study of two chromophores based on 4H-pyranylidene moiety were reported in this research. These chromophores exhibit near-infrared fluorescence, large Stokes shift, enhanced emission, good photostability, high molecular first hyperpolarizabilities, and two-photon absorption cross-sections, showing potential applications in electro-optical devices. Overall, the near-infrared fluorescent chromophores demonstrated versatile materials with a myriad of applications in optoelectronics and biological applications.
MATERIALS ADVANCES
(2021)