Article
Medicine, General & Internal
Dae-Yeon Kim, Hee-Tae Cheong, Chang-Six Ra, Kazuhiro Kimura, Bae Dong Jung
Summary: The study revealed that inhibition of UCP2 expression in Hep3B cells was due to methylation of the promoter region, and treatment with 5-aza could increase UCP2 expression, which may have implications for cancer treatment.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yenan Fu, Xi Zhang, Xiao Liu, Peng Wang, Wenhui Chu, Wei Zhao, Yunling Wang, Guangbiao Zhou, Yu Yu, Hongquan Zhang
Summary: The DNMT1-PAS1-PH20 axis plays an important role in controlling breast cancer growth and metastasis. PAS1 suppresses PH20 expression by recruiting SUV39H1 for methylation, leading to its silencing. Targeting this axis could be a potential therapeutic strategy for breast cancer.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2022)
Article
Medicine, General & Internal
Bader O. O. Almutairi, Mikhlid H. H. Almutairi, Abdulwahed F. F. Alrefaei, Saad Alkahtani, Saud Alarifi
Summary: This study found that HSPB6 expression is downregulated in colorectal cancer tissues and is associated with tumor progression. Furthermore, HSPB6 is methylated in two colorectal cancer cell lines, and demethylation increases its expression, indicating a mechanistic association between DNA methylation and HSPB6. Therefore, HSPB6 could be a promising biomarker for colorectal cancer diagnosis.
MEDICINA-LITHUANIA
(2023)
Article
Medicine, Research & Experimental
Zhehan Chang, Wenqiang Xu, Shuyuan Jiang, Xiaolei Liu, Hongwei Zhu, Peng Wang, Bing Gao, Kerui Gong, Guanghui Guo, Kai Sun, Chunyang Zhang, Ruijuan Han, Guo Shao
Summary: This study found that 5-Aza-CdR can improve learning and memory behavior in mice, while increasing neurogenesis and the expression of the Notch1 pathway in the hippocampus. In vitro experiments also confirmed that this effect may be achieved by increasing the S-phase in cell cycle and cell viability.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Medicine, General & Internal
Xin-Xin Yan, Na Guo, Song-Wei Ru, Zhi-Yuan Wang, Hai-Juan Sui, Yin-Shi Xu, Zhen-Dan Yao
Summary: The incidence and mortality of colon cancer are increasing, and effective biomarkers for its diagnosis are limited. This study aimed to identify the predictors of colon cancer prognosis by detecting the levels of 5mC, 5hmC, 5fC, and 5caC in colon cancer and adjacent non-tumor tissues. The results showed that 5mC and 5hmC can serve as biomarkers for colon cancer and are stable predictors and therapeutic targets.
Article
Immunology
Yanhong Bu, Hong Wu, Ran Deng, Yan Wang
Summary: This study investigated the mechanism of Geniposide (GE) inhibiting angiogenesis in rheumatoid arthritis (RA) and found that GE may inhibit angiogenesis by reducing Dnmt1-mediated PTEN gene hypermethylation, thereby reducing synovitis and angiogenesis in AA rats.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Longxiang Wu, Zhongqing Yang, Guoyu Dai, Benyi Fan, Junbin Yuan, Yalin Liu, Peihua Liu, Zhenyu Ou
Summary: This study reveals the increased expression of SOX5 in bladder cancer and its role in promoting cell growth and migration. It also shows that suppressing SOX5 inhibits the expression of DNMT1 and overexpressing DNMT1 alleviates the cell progress of bladder cancer inhibited by SOX5. The study suggests that SOX5 exerts its functions in bladder cancer cells by modulating the SOX5/DNMT1/p21 pathway.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2022)
Article
Chemistry, Multidisciplinary
Zichen Li, Zebin Tong, Qingyue Gong, Huasong Ai, Shuai Peng, Cong Chen, Guo-Chao Chu, Jia-Bin Li
Summary: Site-selective conjugation chemistry using CAET as a bifunctional handle has been developed to synthesize chemically stable ubiquitin chains. This strategy has been extended to the synthesis of ubiquitinated histones, enabling the study of DNMT1 binding to ubiquitinated nucleosomes and mapping the interaction hotspots. Our work highlights the potential of modern chemical protein synthesis for epigenetic studies.
Article
Chemistry, Multidisciplinary
Changqing Wu, Yu'e Liu, Wenju Liu, Tianhui Zou, Shaojuan Lu, Chengjie Zhu, Le He, Jie Chen, Lan Fang, Lin Zou, Ping Wang, Lihong Fan, Hongxiang Wang, Han You, Juxiang Chen, Jing-Yuan Fang, Cizhong Jiang, Yufeng Shi
Summary: This study identified the NNMT-DNMT1 axis as a critical molecular mechanism determining cancer cell sensitivity to OXPHOS inhibition, and revealed NNMT and DNMT1 as reliable biomarkers for OXPHOS-targeting cancer therapies.
Article
Cell Biology
Bin Xie, Feng Peng, Fengping He, Yixing Cheng, Jiangtao Cheng, Zhibing Zhou, Wei Mao
Summary: RASSF1A gene is highly methylated in lung cancer, and this high methylation level may relieve its resistance to lung cancer by inhibiting the binding of transcription factors to RASSF1A. The loss of CTCF binding to RASSF1A is associated with DNA methylation, and DNA methylation blocks the regulation of RASSF1A expression by CTCF.
CELL BIOLOGY INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Tarek K. Motawi, Shohda A. El-Maraghy, Dina Sabry, Omina M. Nady, Mahmoud A. Senousy
Summary: The study found that cromolyn chitosan nanoparticles (CSNPs) may have the potential as an epigenetic drug by inhibiting ERK1/2 phosphorylation and DNA methylation. In in vitro and in vivo experiments, cromolyn CSNPs showed significant anticancer effects and influenced the expression of DNMT1 and METTL3, impacting DNA and RNA methylation.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Engineering, Environmental
Yibo Zhang, Liu He, Yiqi Yang, Jieqiong Cao, Zijian Su, Bihui Zhang, Huiying Guo, Zhenyu Wang, Peiguang Zhang, Junye Xie, Jieruo Li, Jinshao Ye, Zhengang Zha, Hengyi Yu, An Hong, Xiaojia Chen
Summary: Triclocarban (TCC), a widely used EDC, has been found to cause osteoarthritis (OA) in zebrafish by stimulating the expression of DNMT1 and initiating DNA hypermethylation. This leads to the suppression of type II collagen and other extracellular matrices, resulting in decreased cartilage tissue and narrowing of the intraarticular space, characteristic of OA pathogenesis. The regulation of OA occurrence by TCC is conserved in both zebrafish cartilage tissue and human chondrocytes. DNMT1 is highlighted as a potential therapeutic target for TCC-induced OA.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biology
Ashley K. Wiseman, Rochelle L. Tiedemann, Huihui Fan, Hui Shen, Zachary Madaj, Michael T. McCabe, Melissa B. Pappalardi, Peter A. Jones
Summary: The DNA methylation status of the X-chromosome in cancer cells is often overlooked, but it plays an important role in the epigenetic silencing of cancer-related genes.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xing Wei, Yingxiang Liu, Weijie Hao, Peiwen Feng, Lei Zhang, Hongni Xue, Qunli Zhou, Zekun Guo
Summary: DNA methylation is an important epigenetic modification involved in various biological processes. PGC7 is a maternal factor that maintains DNA methylation during early embryonic development. This study identified a mechanism by which PGC7 regulates genome-wide DNA methylation through the phosphorylation of DNMT1 at ser717 by ERK. These findings provide new insights into DNA methylation-related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Iryna Ivasyk, Leonora Olivos-Cisneros, Stephany Valdes-Rodriguez, Marie Droual, Hosung Jang, Robert J. J. Schmitz, Daniel J. C. Kronauer
Summary: While the function of DNA methylation in arthropods is poorly understood, studies in eusocial insects have suggested its role in caste development. However, the findings are inconsistent and controversial. By using CRISPR/Cas9 to mutate DNMT1 in clonal raider ants, the authors demonstrate that ants can undergo normal development without DNMT1 or DNA methylation, contrary to mammals. They also find no evidence of DNA methylation regulating caste development, but show that DNMT1 plays a crucial but unknown role in the insect germline.
NATURE COMMUNICATIONS
(2023)