Journal
APPLIED SCIENCES-BASEL
Volume 9, Issue 19, Pages -Publisher
MDPI
DOI: 10.3390/app9194129
Keywords
pirogue; biodeterioration; high-throughput sequencing; culture-dependent approach; cellulase activity
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Funding
- Natural Science Foundation of Tianjin [19JCZDJC33700]
- Fundamental Research Funds for the Central Universities, Nankai University [63181211]
- National Key RAMP
- D Program of China [2017YFE013040]
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The goal of this research was to analyze the fungal community responsible for the biodeterioration of a pirogue in the National Maritime Museum of China and to make recommendations for the protection of this artifact. Molecular identification of fungal strains isolated from the surface of the pirogue and the air of the storage room that were most closely related to Cladosporium, Penicillium, Talaromyces and Trichoderma spp. DNA extracted from the samples was sequenced on the Illumina MiSeq platform. The results showed that the predominant fungal genera present were Penicillium sp., Cladosporium sp. and Exophiala sp. Thereafter, cellulose degradation experiments were carried out on the predominant fungi screened by pure culturing. Finally, we tested the sensitivity of the predominant fungal isolates to four biocides. This work suggests that we should pay more attention to Penicillium sp. and Cladosporium sp. in the protection of wooden artifacts, and environmental control is recommended as the main means of protecting the pirogue.
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