Article
Polymer Science
Yang Ji, Mengdie Hou, Jin Zhang, Meiqi Jin, Tianlin Wang, Huazhe Yang, Xiaodong Zhang
Summary: In the field of bone repair, the inorganic-organic composite scaffold is a promising strategy. This study prepared a porous zirconia matrix and a partially degradable ZrO2-chitosan particles-GelMA composite scaffold. The results showed that the chitosan/GelMA ratio affected the mechanical properties, swelling properties, and degradation behaviors of the composite scaffold, and the composite scaffold also exhibited improved cell proliferation performance.
Article
Biochemistry & Molecular Biology
Konstantinos Loukelis, Foteini Machla, Athina Bakopoulou, Maria Chatzinikolaidou
Summary: This study investigates the impact of kappa-carrageenan on dental pulp stem cells and reveals that its integration in scaffolds significantly enhances the odontogenic potential of DPSCs, supporting dentin-pulp regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Biomaterials
Lijing Wang, Yanyan Cao, Zhizhong Shen, Meng Li, Wendong Zhang, Yu Liu, Yating Zhang, Jiahui Duan, Zhuwei Ma, Shengbo Sang
Summary: In this study, a composite scaffold composed of GelMA and CMCS was fabricated, and its potential to promote angiogenesis was proven. The biocompatibility, cell distribution, and endothelial differentiation of the scaffold were evaluated using various methods. The results showed that the composite scaffold exhibited excellent mechanical properties, CD31 expression, and vasculogenic gene expression. Furthermore, the activity of cells-laden composite scaffold in 3D printing demonstrated the feasibility of bioprinting.
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Shih-Kai Wang, Hong Zhang, Yin-Lin Wang, Hung-Ying Lin, Figen Seymen, Mine Koruyucu, J. Timothy Wright, Jung-Wook Kim, James P. Simmer, Jan C. -C. Hu
Summary: This study identified disease-causing mutations in FAM20A and revealed that upregulation of BMP signaling underlies intrapulpal calcifications in ERS.
INTERNATIONAL ENDODONTIC JOURNAL
(2023)
Article
Engineering, Biomedical
Xinna Wang, Bo Gao, Barbara P. Chan
Summary: The study presents a mask-free, non-contact and biocompatible multiphoton microfabrication and micropatterning technology for engineering soluble cell niches. Immobilizing biotinylated BMP-2 on micro-printed neutravidin micropatterns resulted in a more sustained and higher level of downstream signaling in C2C12 cells. This work establishes a universal platform for engineering cell niches and contributes to modeling signal transduction and drug screening using native soluble cell niches.
Article
Engineering, Biomedical
Lya Piaia, Simone S. Silva, Joana M. Gomes, Albina R. Franco, Emanuel M. Fernandes, Flavia C. M. Lobo, Luisa C. Rodrigues, Isabel B. Leonor, Marcio C. Fredel, Gean Salmoria, Dachamir Hotza, Rui L. Reis
Summary: Tissue engineering provides new approaches to manage bone regeneration by introducing scaffolds that can be autografts and/or allografts. The chitosan/beta-tricalcium phosphate-based scaffolds developed in this study have excellent biological and mechanical performance, making them suitable for bone tissue engineering.
BIOMEDICAL MATERIALS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Kao Li, Rita O'Dwyer, Fan Yang, Jerome Cymerman, Juyi Li, Jatre D. Feldman, Marcia Simon, Miriam Rafailovich
Summary: This in vitro study evaluates cross-linked hydrogels with and without the addition of fibrin as potential scaffold materials for endodontic regeneration. The results demonstrate that the hybrid gel scaffold enhances cell migration, promotes odontogenic differentiation, and increases biomineralization. The mechanical properties of the hybrid gel also facilitate stem cell migration and subsequent differentiation, which are crucial for regenerative procedures. Overall, the findings suggest that the insertion of the hybrid gelatin-fibrin scaffold could lead to more reproducible outcomes in regenerative endodontic treatment. Rating: 9/10.
Article
Energy & Fuels
Kurnia Wiji Prasetiyo, Dede Hermawan, Yusuf Sudo Hadi, Putri Amanda, Sukma Surya Kusumah, Subyakto
Summary: Water-soluble chitosan (WSC) has been developed through a pressurized hydrolysis process and has a wide range of applications. Studies have shown that WSC as a sizing agent in paper composites improves mechanical properties and exhibits antimicrobial activity. However, high concentrations of WSC may have inhibitory effects on certain bacteria.
BIOMASS CONVERSION AND BIOREFINERY
(2021)
Article
Agricultural Engineering
Tian He, Lixue Hang, Mingyou Liu, Haiyu Liu, Lu Wang, Fangeng Chen, Xiaofei Tian
Summary: The research showed that water-soluble chitosan effectively protects cellulose viscosity during the ozone bleaching process, leading to improved pulp quality, especially for sulfate hardwood pulp.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Georgia Vagropoulou, Maria Trentsiou, Anthie Georgopoulou, Eleni Papachristou, Oleg Prymak, Aristeidis Kritis, Matthias Epple, Maria Chatzinikolaidou, Athina Bakopoulou, Petros Koidis
Summary: This study demonstrates the potential of hybrid chitosan/gelatin/nanohydroxyapatite scaffolds to support viability and proliferation of dental pulp stem cells, inducing odontogenic differentiation and in vitro biomineralization without the addition of any inductive factors. The results suggest a promising strategy for tissue engineering of mineralized dental tissues.
Article
Chemistry, Applied
Davide Porrelli, Martina Gruppuso, Federica Vecchies, Eleonora Marsich, Gianluca Turco
Summary: The study demonstrated that porous alginate/hydroxyapatite scaffolds with a bioactive coating can enhance the healing of bone defects and improve the osteoinductive and osteoconductive properties of the scaffolds. The bioactive coating can enhance the adhesion of human dental pulp stem cells on the scaffolds, while also stimulating the osteogenic differentiation of the cells.
CARBOHYDRATE POLYMERS
(2021)
Article
Engineering, Biomedical
Hongmei Ding, Xu Peng, Xiaoshuang Yu, Mengyue Hu, Chang Wan, Ningning Lei, Yihao Luo, Xixun Yu
Summary: By constructing calcium polyphosphate scaffolds (CPP) with a chitosan-grafted reduced graphene oxide coating (CPP/CS-rGO), the biomineralization and antibacterial activity of the scaffold were significantly improved. The CS-rGO coating also stimulated osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and enhanced interfacial osseointegration, leading to better bone regeneration.
BIOMEDICAL MATERIALS
(2022)
Article
Materials Science, Multidisciplinary
Jingwen Liu, Tingting Cui, Xiaowei Xu, Yu Du, Lin Wang, Su Chen, Jie Pang
Summary: This study fabricated inexpensive silk sericin protein-based artificial skin nanofiber scaffolds with excellent biological activity and mechanical strength using microfluidic blow-spinning technology. It demonstrated good antibacterial effects and degradability properties, and showed potential applications in promoting wound healing and skin regeneration.
ADVANCED FIBER MATERIALS
(2023)
Article
Materials Science, Biomaterials
Alessandra Soriente, Soledad Perez Amodio, Ines Fasolino, Maria Grazia Raucci, Christian Demitri, Elisabeth Engel, Luigi Ambrosio
Summary: The study aimed to evaluate the bioactive chitosan/poly(ethylene glycol) diacrylate scaffolds in promoting in vitro angiogenesis process through organic and inorganic cues for scaffold bioactivation. The results highlighted the positive effect of bioactive signals on the scaffold surface in enhancing angiogenic response.
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Shalini Dasgupta, Kolimi Prashanth Reddy, Pallab Datta, Ananya Barui
Summary: Effective vascularization during wound healing is a challenging task for skin tissue regeneration. This study reports the potential vascularization property of a chitosan-collagen-fibrinogen scaffold incorporating vitamin D3 (CCF-D3), which induces the transition of dental pulp mesenchymal stem cells (MSC) to endothelial lineage. MSC seeded on UV-exposed CCF-D3 scaffolds showed higher cell viability and transitioned towards endothelial lineage as indicated by increased gene expressions and flow cytometric analysis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Physical
Felix J. Geissel, Varvara Platania, Alexander Gogos, Inge K. Herrmann, Georgios N. Belibasakis, Maria Chatzinikolaidou, Georgios A. Sotiriou
Summary: This study demonstrates that the concentration of Ag ions in solution is the major factor driving the antibiofilm effect of nanosilver coatings, independent of Ag size and coating thickness. The antibiofilm effect predominantly stems from the released Ag ions rather than direct bacterial contact inhibition, especially for coatings featuring the smallest Ag particle sizes.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Engineering, Biomedical
F. Machla, V Sokolova, V Platania, O. Prymak, K. Kostka, B. Kruse, M. Agrymakis, S. Pasadaki, A. Kritis, K. Alpantaki, M. Vidaki, M. Chatzinikolaidou, M. Epple, A. Bakopoulou
Summary: Tissue engineering provides new treatment approaches for regenerating lost dental tissues, and this study investigated the characteristics of EDTA-treated, freeze-dried dentin matrices (HTFD scaffolds) conditioned with calcium phosphate nanoparticles (NPs) bearing dentinogenesis-inducing factors. The synthesized NPs showed efficient transfection of dental pulp stem cells (DPSCs), and HTFD/NPs constructs induced odontogenic shift of DPSCs, preserving cell viability and leading to the reconstruction of the ortho-dentin/odontoblastic layer barrier.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Multidisciplinary
Kostas Parkatzidis, Nghia P. Truong, Richard Whitfield, Chiara E. Campi, Benjamin Grimm-Lebsanft, Soren Buchenau, Michael A. Rubhausen, Simon Harrisson, Dominik Konkolewicz, Siegfried Schindler, Athina Anastasaki
Summary: In controlled radical polymerization, oxygen is typically seen as undesirable, but in this study, it was found that oxygen can actually trigger a faster and more efficient polymerization reaction. By using a special mechanism called superoxido ARGET-ATRP, the researchers achieved high-end group fidelity and near-quantitative conversions in all steps of the reaction. The method showed versatile applications and was not affected by the purity of the starting catalyst, even with a highly oxidized reagent.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Polymer Science
Valentina Bellotti, Kostas Parkatzidis, Hyun Suk Wang, Nethmi De Alwis Watuthanthrige, Matteo Orfano, Angelo Monguzzi, Nghia P. P. Truong, Roberto Simonutti, Athina Anastasaki
Summary: Retrieving starting monomers from polymers synthesized by reversible deactivation radical polymerization has been proven effective in increasing recyclability and enabling industrial implementation. A novel method involving the use of Eosin Y under light irradiation has been discovered to trigger faster depolymerization even at lower temperatures. The activation of a macroCTA by Eosin Y results in accelerated macroradical generation, significantly improving current depolymerization approaches.
Article
Chemistry, Multidisciplinary
Felix J. Geissel, Varvara Platania, Niccolo DeBerardinis, Charlotte Skjoldebrand, Georgios N. Belibasakis, Cecilia Persson, Gry Hulsart-Billstrom, Maria Chatzinikolaidou, Georgios A. Sotiriou
Summary: Bone implants often fail due to bacterial colonization and loose biofilm infections. A novel nanoparticle coating composed of silver and bioglass has been developed to address these issues. The coating is produced using a scalable and reproducible process and exhibits both antibacterial and osteogenic properties. The silver-ion release inhibits biofilm formation, while the coating maintains high biocompatibility.
ADVANCED MATERIALS INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Konstantinos Loukelis, Foteini Machla, Athina Bakopoulou, Maria Chatzinikolaidou
Summary: This study investigates the impact of kappa-carrageenan on dental pulp stem cells and reveals that its integration in scaffolds significantly enhances the odontogenic potential of DPSCs, supporting dentin-pulp regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Multidisciplinary
Glen R. Jones, Hyun Suk Wang, Kostas Parkatzidis, Richard Whitfield, Nghia P. Truong, Athina Anastasaki
Summary: Controlled polymerization methods allow for the precise design and preparation of polymeric materials. Recent work has shown that these methods also enable depolymerization of polymers under mild conditions. This perspective focuses on depolymerization from polymers synthesized by controlled polymerizations, such as radical, ionic, and metathesis polymerizations, and explores concepts to enhance depolymerization.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Polymer Science
Georgia-Ioanna Kontogianni, Amedeo Franco Bonatti, Carmelo De Maria, Raasti Naseem, Priscila Melo, Catarina Coelho, Giovanni Vozzi, Kenneth Dalgarno, Paulo Quadros, Chiara Vitale-Brovarone, Maria Chatzinikolaidou
Summary: Bone tissue engineering is a promising strategy for treating bone-related disorders. A novel polymeric blend of PLLA, PCL, and PHBV was explored to address the trade-off between mechanical properties and bioactivity. The blend was enriched with nano-HA and Sr-nano-HA to enhance its osteogenic potential.
Article
Chemistry, Multidisciplinary
Georgia-Ioanna Kontogianni, Catarina Coelho, Remy Gauthier, Sonia Fiorilli, Paulo Quadros, Chiara Vitale-Brovarone, Maria Chatzinikolaidou
Summary: Nanohydroxyapatite (nanoHA) is a highly biocompatible and osteoconductive material for bone regeneration. By enriching nanoHA with strontium ions, enhanced mechanical properties and biological activity can be achieved. In this study, nanoHA and nanoHA with different substitution degrees of calcium with strontium ions (Sr-nanoHA_50 and Sr-nanoHA_100) were evaluated for their cytotoxicity and osteogenic potential. All three nanoHA-based materials showed cytocompatibility, needle-shaped nanocrystals, and enhanced osteogenic activity. Gene expression analysis demonstrated significant upregulation of osteocalcin, osteonectin, and osteopontin in all three compositions compared to the control. These findings indicate the great potential of the produced compounds for bone regeneration.
Article
Biotechnology & Applied Microbiology
Georgia-Ioanna Kontogianni, Konstantinos Loukelis, Amedeo Franco Bonatti, Elisa Batoni, Carmelo De Maria, Raasti Naseem, Kenneth Dalgarno, Giovanni Vozzi, David B. MacManus, Subrata Mondal, Nicholas Dunne, Chiara Vitale-Brovarone, Maria Chatzinikolaidou
Summary: In this study, mechanical stimulation was applied to bone tissue engineering constructs to mimic the dynamic nature of bone. The experiments showed that cyclic uniaxial compression at different frequencies significantly enhanced the activity and osteogenic capacity of osteoblastic cells. The frequency of 0.5 Hz showed the highest response.
BIOENGINEERING-BASEL
(2023)
Article
Chemistry, Multidisciplinary
Kostas Parkatzidis, Nghia P. Truong, Krzysztof Matyjaszewski, Athina Anastasaki
Summary: This paper introduces a photocatalytic ATRP depolymerization method, which significantly reduces the reaction temperature while enabling temporal regulation and high monomer recovery through the use of low-toxicity catalysts and visible light irradiation. The depolymerization rate can be modulated by simply turning the light on and off, and the method is compatible with various polymers and light sources while preserving the end-group fidelity.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Polymer Science
Sina Della Casa, Kostas Parkatzidis, Nghia P. Truong, Athina Anastasaki
Summary: Oxygen-enhanced atom transfer radical polymerization (ATRP) via the formation of a superoxido copper complex has been proven to be a powerful tool for synthesizing well-defined polymers. UV irradiation was found to significantly enhance the polymerization rate and achieve near-quantitative monomer consumption and narrow molar mass distributions. The UV-induced fast reduction of the superoxido copper complex was identified as the cause of the rate acceleration.
JOURNAL OF POLYMER SCIENCE
(2023)
Review
Polymer Science
Konstantinos Loukelis, Zina A. Helal, Antonios G. Mikos, Maria Chatzinikolaidou
Summary: Bioprinting aims to regenerate damaged human tissues by printing live cells and biocompatible materials. Polymeric hydrogels are commonly used as ink materials for 3D and 4D bioprinting, and the incorporation of nanoparticles allows for tunability of material properties. This paper reviews the incorporation of nanoparticles and other nanoscale additives in printable bioinks for tissue engineering applications, specifically in bone, cartilage, dental, and cardiovascular tissues. The interactions between cells and materials, as well as the formulation methodologies for bioprinting, are discussed.
Article
Chemistry, Multidisciplinary
Kostas Parkatzidis, Nghia P. Truong, Krzysztof Matyjaszewski, Athina Anastasaki
Summary: A photocatalytic ATRP depolymerization method is introduced, which lowers the reaction temperature and enables temporal control, achieving high monomer recovery and end-group fidelity.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Medicinal
Maria Chatzinikolaidou, Cristina Nativi, Oscar Francesconi, Francisco Corzana, Georgia-Ioanna Kontogianni, Giorgio Pesciullesi, Roberta Gualdani, Claudiu T. Supuran, Andrea Angeli, Rafaela Maria Kavasi
Summary: This study describes the impact of a novel TRPA1 inhibitor on human osteosarcoma cells and demonstrates a potential new strategy for treating osteosarcomas.
ACS PHARMACOLOGY & TRANSLATIONAL SCIENCE
(2022)