Article
Microbiology
Carol M. Romo-Barrera, Laura E. Castrillon-Rivera, Alejandro Palma-Ramos, Jorge I. Castaneda-Sanchez, Julieta Luna-Herrera
Summary: The research found that macrophages have a certain resistance to infection by probiotics such as Bacillus, using METs for self-clearance. Additionally, Bacillus can induce macrophages to release extracellular DNA, thereby inhibiting the growth of Staphylococcus aureus.
Article
Environmental Sciences
Nuan Song, Wei Wang, Yue Wang, Yalin Guan, Shiwen Xu, Meng-Yao Guo
Summary: The study investigated the effects of H2S exposure on chicken trachea, revealing airway injury and METs release. The results indicate that H2S mediates the release of METs through miRNA-156-5p targeting ATF2, leading to tracheal inflammatory injury.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Review
Cell Biology
Waywen Loh, Sonja Vermeren
Summary: This minireview focuses on the anti-inflammatory functions of neutrophils, with particular emphasis on recent findings related to neutrophil cell death, as well as features associated with resolution of inflammation and repair.
Article
Immunology
Xianlong Chen, Heng Ma, Shengwei Mo, Shuangni Yu, Zhaohui Lu, Jie Chen
Summary: This study investigates the clinical significance of tumor-infiltrating neutrophils and macrophages, as well as the related extracellular traps (ETs), in pancreatic ductal adenocarcinoma (PDAC). The results show that neutrophil infiltration and ET formation are associated with patient prognosis and response to chemotherapy.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Environmental Sciences
Dongliu Luo, Jintao Zhang, Hang Yin, Shanshan Li, Shiwen Xu, Shu Li
Summary: This study investigates the effects of perfluorooctane sulphonate (PFOS) on liver injury and the potential protective role of cannabidiol (CBD). Results showed that PFOS exposure induces inflammatory infiltration, macrophage extracellular trap (MET) formation, and fibrosis in the liver. In vitro experiments also demonstrated that PFOS can induce inflammation and fibrosis marker gene expression. However, CBD treatment was found to alleviate PFOS-induced liver injury by inhibiting MET release through interaction with PAD4.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Biochemistry & Molecular Biology
Hiroyuki Tada, Takashi Nishioka, Rina Ishiyama, Li-Ting Song, Sakura Onoue, Kazuyoshi Kawahara, Eiji Nemoto, Kenji Matsushita, Shunji Sugawara
Summary: In this study, the effects of mast cell extracellular traps (MCETs) released from mast cells after infection with periodontal pathogen Fusobacterium nucleatum were investigated. F. nucleatum induced the release of MCETs from mast cells, and these MCETs expressed macrophage migration inhibitory factor (MIF). Importantly, MIF bound to MCETs induced the production of proinflammatory cytokines by monocytic cells. These findings suggest that MIF expressed on MCETs released upon infection with F. nucleatum promotes inflammatory responses associated with the pathogenesis of periodontal disease.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Review
Oncology
Jiping Li, Yuying Liu, Yidong Yuan, Bo Huang
Summary: Cellular mechanics is a major regulating factor of cellular architecture and biological functions, responding to intrinsic and extrinsic forces in the microenvironment. It plays a fundamental role in complicated immune responses and can be characterized using atomic force microscopy (AFM). AFM applications include studying neutrophil-extracellular-trap release, immunological functions of macrophages, and membrane pore formation.
FRONTIERS OF MEDICINE
(2021)
Article
Cell Biology
Dominika Drab, Michal Santocki, Malgorzata Opydo, Elzbieta Kolaczkowska
Summary: Macrophage extracellular traps (METs) are a novel defense mechanism used by macrophages to combat microbial infections. The formation of METs is still not well understood, but researchers have successfully developed a protocol to induce MET formation using bone marrow-derived macrophages (BMDMs) obtained from cryopreserved cells. They found that BMDMs were capable of forming METs composed of extracellular DNA and attached proteins. Additionally, they discovered that reactive nitrogen species (RNS) such as nitric oxide (NO) were involved in the formation of METs.
CELL AND TISSUE RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Kaiyun Yang, Mingtan Tang, Hao-Han Chang, Manju Kanamala, Alan James Davidson, Zimei Wu
Summary: Comparative study between non-pH-sensitive liposomes and mannosylated pH-sensitive liposomes for protein delivery to macrophages revealed that mannosylation enhanced cellular uptake and endosomal escape without affecting pH-responsiveness. GFP fluorescence acted as a probe for endosomal escape of liposomal cargo, showing efficient performance of mannosylated liposomes in facilitating intracellular delivery.
PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY
(2021)
Review
Dentistry, Oral Surgery & Medicine
Iain L. C. Chapple, Josefine Hirschfeld, Alpdogan Kantarci, Asaf Wilensky, Lior Shapira
Summary: Neutrophils, also called granulocytes, are myeloid cells that play a critical role in acute and chronic inflammation, as well as wound healing. They express a variety of surface receptors to facilitate their migration, recognition, and destruction of pathogens or damaged tissues. Neutrophils can undergo different forms of programmed cell death and exhibit heterogeneous behavior and biological repertoire.
PERIODONTOLOGY 2000
(2023)
Article
Biochemistry & Molecular Biology
Konstantina Flouda, John Mercer, Michael J. Davies, Clare L. Hawkins
Summary: This study demonstrates that HOCl reacts rapidly with cells, causing cell death, while HOSCN reacts more slowly, with cell death occurring only after prolonged incubation. HOCl also influences mitochondrial respiration, decreases glycolysis, lactate release, ATP production, cellular thiols, and glutathione levels.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Clare L. Hawkins, Michael J. Davies
Summary: The heme peroxidase family, especially MPO, generates powerful oxidants for pathogen removal but also causes tissue damage and inflammatory diseases. MPO binds to extracellular biomolecules, leading to localized damage on specific sites and species, affecting cellular dysfunction and altered gene expression. MPO's interaction with damaged ECM components may explain its accumulation at sites of inflammation.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Multidisciplinary Sciences
Luke Hall, Chaouri Guo, Sarah Tandy, Kathryn Broadhouse, Anthony C. Dona, Ernst Malle, Emil D. Bartels, Christina Christoffersen, Stuart M. Grieve, Gemma Figtree, Clare L. Hawkins, Michael J. Davies
Summary: Elevated levels of the MPO substrate SCN-, which can be modulated by dietary means, can protect against acute ischemia-reperfusion injury by reducing infarct size and increasing salvageable area. Additionally, it can improve cardiac function and decrease antibody recognition of HOCl-damaged myocardial proteins.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Kristian H. Rasmussen, Clare L. Hawkins
Summary: Macrophages play a crucial role in innate immunity and inflammation regulation. They can release extracellular traps (METs) with antimicrobial properties, which are also implicated in disease pathology. Additionally, macrophages are involved in the clearance of extracellular traps, aiding in the resolution of tissue damage.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2022)
Article
Biochemistry & Molecular Biology
Shuqi Xu, Christine Y. Chuang, Ernst Malle, Luke F. Gamon, Clare L. Hawkins, Michael J. Davies
Summary: Myeloperoxidase (MPO) generates the bactericidal oxidant hypochlorous acid (HOCl) to destroy pathogens, but it also causes tissue damage and disease. This study found that plasma levels of thiocyanate (SCN-) can modulate MPO-mediated damage, while nitrite (NO2-) alters the type of protein modification.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Editorial Material
Immunology
Dragana Odobasic, Clare Louise Hawkins, Marko Radic
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Line A. E. Hallberg, Nicoline W. Thorsen, Els A. Hartsema, Per M. Hagglund, Clare L. Hawkins
Summary: Histones play a critical role in packaging nuclear DNA and chromatin assembly. They can undergo a range of post-translational modifications, which affect their regulatory function. Histones can also be released into the extracellular environment through pathways such as neutrophil extracellular traps. This study investigates the reactivity of histones with hypochlorous acid and the potential implications for chronic inflammatory diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Yihe Wang, Christine Y. Chuang, Clare L. Hawkins, Michael J. Davies
Summary: Matrix metalloproteinase-9 (MMP9) plays a crucial role in the degradation of extracellular matrix (ECM) proteins. This study demonstrates that MMP9 can be activated by both chloramines and oxidation. The activation of MMP9 and ECM degradation are interdependent processes that may contribute to the progression of diseases.
Article
Multidisciplinary Sciences
Jianfei He, Jonas Hyld Steffen, Peter Waaben Thulstrup, Jannik Nedergaard Pedersen, Max B. Sauerland, Daniel E. Otzen, Clare L. Hawkins, Pontus Gourdon, Michael J. Davies, Per Hagglund
Summary: This study found that anastellin affects ECM processing and cytokine secretion in smooth muscle cells, with slightly different effects observed between the wild-type and mutant forms of anastellin.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Mathias Jensen, Nicoline W. Thorsen, Line A. E. Hallberg, Per Hagglund, Clare L. Hawkins
Summary: Neutrophil extracellular trap (NET) release plays a key role in chronic diseases like atherosclerosis, but the release of macrophage extracellular traps (METs) and their composition are less understood. This study investigated MET release from human THP-1 macrophages exposed to different stimuli and discovered that METs were composed of histones, various proteins involved in different cellular processes, and the absence of proteases. These findings provide new insights into the implications of MET formation in immune defense and pathology.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Yihe Wang, Astrid Hammer, Gerald Hoefler, Ernst Malle, Clare L. Hawkins, Christine Y. Chuang, Michael J. Davies
Summary: Atherosclerosis is a chronic inflammatory disease characterized by plaque formation in arterial walls. The study found that hypochlorous acid (HOCl) and chloramines can specifically modify aggrecan, a proteoglycan involved in atherogenesis. These modifications, different from those induced by ADAMTS1, can lead to irreversible protein cross-links. The findings also showed colocalization of aggrecan and HOCl-generated epitopes in advanced human atherosclerotic plaques.
Article
Biochemistry & Molecular Biology
Line A. E. Hallberg, Kristine Barlous, Clare L. Hawkins
Summary: Extracellular traps, known as NETs, are released by neutrophils and other immune cells as part of the innate immune response and are associated with various pathological conditions. This study investigated the efficacy of different antioxidant approaches in modulating the release of NETs. The results showed that thiocyanate, selenocyanate, and nitroxides could prevent NETosis in neutrophils when exposed to certain stimuli. These findings provide new insights into the potential use of MPO inhibitors and HOCl scavengers to prevent pathological NETosis.
Article
Biochemistry & Molecular Biology
Jianfei He, Christine Y. Chuang, Clare L. Hawkins, Michael J. Davies, Per Hagglund
Summary: The extracellular matrix (ECM) of tissues is formed by proteins, proteoglycans, and glycosaminoglycans, and it plays a crucial role in maintaining tissue integrity. Peroxynitrite, an oxidant produced during inflammation, can modify fibronectin and impair its function. This study investigated the effects of peroxynitrite on the structure of ECM and its interactions with cellular components. The results suggest that peroxynitrite can influence ECM structure and may have implications in pathological conditions such as atherosclerosis.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Shuqi Xu, Christine Y. Chuang, Clare L. Hawkins, Per Hagglund, Michael J. Davies
Summary: Peroxynitrous acid/peroxynitrite (ONOOH/ONOO ¡) is a powerful oxidizing/nitrating system formed at sites of inflammation, which can modify biological targets, particularly proteins. This study found that multiple proteins from primary human coronary artery smooth muscle cells are nitrated, with various modifications occurring on cellular and extracellular matrix proteins. The nitration occurs both endogenously and exogenously, and it may have significant effects on cell and protein function, potentially contributing to the development and exacerbation of diseases such as atherosclerosis.
Meeting Abstract
Cardiac & Cardiovascular Systems
M. Jensen, P. Hagglund, Y. Zhang, B. Rayner, C. L. Hawkins