Article
Agricultural Engineering
Hong-Sheng Qing, Jie-Yu Qian, Jia-Hong Chen, Ling-Li Jiang, Jian-Xin Fu, Xing-Qi Huang, Chao Zhang
Summary: This study analyzed the content and composition of carotenoids in common zinnia, and found that cyclic carotenoids were the main pigment in both cultivars, with a higher total carotenoid content in DY flowers. Several key genes related to carotenoid biosynthesis were identified and their functions were confirmed through cloning experiments.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Agriculture, Multidisciplinary
Bing Zhang, Tianxiang Wen, Nan Xiang, Yihan Zhao, Xinbo Guo
Summary: Ultrasonic pretreatment was found to increase the accumulation of tocopherols and carotenoids in maize seedlings but decrease tocotrienol synthesis. The carotenoid content reached its highest level at 8 minutes of ultrasonic pretreatment, while tocopherol initially dropped but began to rise after 8 minutes. The predominant component of tocochromanols in maize seedlings shifted from gamma-tocotrienol to alpha-tocopherol after ultrasonic pretreatment.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Plant Sciences
Jingwen Yang, Jiazhi Huang, Xi Wu, Yun Xu, Zheng Gu, Yajue Chen, Yu Zhang, Yujun Ren, Ying Miao
Summary: This study identified the transcription factor NtMYB1 as a key regulator of petal senescence and leaf yellowing in Narcissus. By targeting the promoters of NtNCED1/2 and activating their transcription, NtMYB1 redirects metabolic flux and regulates the biosynthesis of abscisic acid and β-carotene.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Horticulture
Qinghao Wang, Luyang Jing, Yue Xu, Weiwei Zheng, Wangshu Zhang
Summary: This study investigated the different pigments and the regulatory mechanisms of differential gene expression between red and yellow fruits in sweet cherry. It was found that the expression levels of anthocyanin genes were significantly higher in red fruits than in yellow fruits, while the expression of carotenoid genes showed more stability in yellow fruits. Therefore, the coloration of sweet cherry is primarily attributed to differential expression of anthocyanin-related genes.
Article
Biochemistry & Molecular Biology
Yue Yin, Hongyan Shi, Jia Mi, Xiaoya Qin, Jianhua Zhao, Dekai Zhang, Cong Guo, Xinru He, Wei An, Youlong Cao, Jianhua Zhu, Xiangqiang Zhan
Summary: This study provides insights into the characteristics of the LbaBBX gene family in wolfberry. The LbaBBX genes are highly responsive to light, phytohormones, and stress conditions, and have orthologs in tomato, eggplant, pepper, and Arabidopsis. LbaBBX2 and LbaBBX4 play key roles in the regulation of carotenoid biosynthesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
ChengGe Zhang, HuanHuan Liu, YaXian Zong, ZhongHua Tu, HuoGen Li
Summary: In this study, the LtuGGPPS2 gene and its promoter were cloned from L. tulipifera, showing high homology to GGPPS genes of other plants and likely involvement in carotenoid and chlorophyll synthesis. Expression was highest in petals, and GUS staining indicated responsiveness to hormones. Overexpression increased carotenoid content and GGPPS enzyme activity, suggesting LtuGGPPS2 as a key terpenoid synthase in L. tulipifera flowers.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Plant Sciences
Rosa Us-Camas, Margarita Aguilar-Espinosa, Jacobo Rodriguez-Campos, Alba Adriana Vallejo-Cardona, Victor Manuel Carballo-Uicab, Hugo Serrano-Posada, Renata Rivera-Madrid
Summary: In this study, the activity of BoCCD enzymes in the biosynthesis of bixin aldehyde from lycopene in B. orellana was determined. It was also found that some BoCCD enzymes can oxidize bixin aldehyde to norbixin.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Horticulture
Yiming Zhang, Yi Zhou, Yijing Wu, Junhuo Cai, Yiqiang Zhang, Jincai Shen, Shibin Wu, Wenjie Liu, Ming Cai, Tangren Cheng, Qixiang Zhang
Summary: Through pigment metabolite analysis and comparative transcriptome analysis, the key pigment difference and its molecular mechanism of accumulation in Chinese narcissus yellow-flowered mutant compared to the white-flowered cultivars were revealed. The mutant had a significantly higher total carotenoid content and exhibited opposite gene expression pattern in the carotenoid biosynthesis pathway compared to the white-flowered cultivars, which likely contributed to carotenoid accumulation in the mutant's tepals.
Article
Multidisciplinary Sciences
Hongxia Song, Qiang Lu, Leiping Hou, Meilan Li
Summary: The study demonstrates that increasing CO2 concentration can enhance biomass and carotenoid content in carrots. By identifying genes related to carotenoids and verifying their activities, the molecular mechanism of this enhancement was elucidated.
SCIENTIFIC REPORTS
(2021)
Article
Plant Sciences
Hee Ju Yoo, Mi-Young Chung, Hyun-Ah Lee, Soo-Bin Lee, Silvana Grandillo, James J. Giovannoni, Je Min Lee
Summary: Carotenoids and apocarotenoids play important roles in pigmentation and flavor production in plants, contributing to their aesthetic and nutritional value. This study identifies carotenoid cleavage dioxygenase 4 (CCD4) as the factor responsible for controlling the dark orange color of tomato fruit. Overexpression of CCD4b leads to changes in carotenoid and apocarotenoid levels in ripe fruit, indicating feedback regulation in the pathway. The findings provide valuable insights into improving the quality and understanding the regulatory mechanisms of carotenoid and apocarotenoid biogenesis in tomato.
Article
Plant Sciences
Xiaoguang Yan, Weiguo Li, Dongmei Liang, Guangrong Zhao, Qinggele Caiyin, Jianjun Qiao
Summary: Through comparative transcriptome analysis, two sesquiterpene synthases were identified in Leonurus sibiricus. LsSqTPS2 was found to produce a high titer of δ-cadinene, which is the major component of L. sibiricus leaf essential oil. This study contributes to understanding sesquiterpene biosynthesis in L. sibiricus.
Article
Plant Sciences
Ramaraj Sathasivam, Hyeon Ji Yeo, Chang Ha Park, Minsol Choi, Haejin Kwon, Ji Eun Sim, Sang Un Park, Jae Kwang Kim
Summary: This study identified and analyzed the carotenoid biosynthesis pathway genes of Chelidonium majus. The genes showed tissue-specific expression, with the highest levels in flowers. The study provides insights for optimizing carotenoid production in C. majus and lays a foundation for further molecular genetics and functional analysis studies.
Article
Biochemical Research Methods
Guangyu E. Chen, C. Neil Hunter
Summary: In this study, the researchers successfully engineered the biosynthetic pathway of bacteriochlorophyll a (BChl a) in Escherichia coli, using coexpression of 18 genes. They identified the bchNBL genes as responsible for the protochlorophyllide reduction step and utilized the same module to modify the previously constructed pathway for chlorophyll a (Chl a) synthesis. Furthermore, they demonstrated the possibility of synthesizing multiple families of photosynthetic pigments in one host by engineering E. coli strains to produce not only BChl a but also carotenoids neurosporene and β-carotene.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Qiannan Diao, Shoubo Tian, Yanyan Cao, Dongwei Yao, Hongwei Fan, Yongping Zhang
Summary: This study analyzed the transcriptome of two different-colored melon varieties and identified 33 genes related to carotenoid metabolism that are important for flesh color formation.
SCIENTIFIC REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Samuel Hendry, Stephan Steinke, Kathrin Wittstein, Marc Stadler, Kirsten Harmrolfs, Yetunde Adewunmi, Gyan Sahukhal, Mohamed Elasri, Linda Thomashow, David Weller, Olga Mavrodi, Wulf Blankenfeldt, Dmitri Mavrodi
Summary: This study identified phenazine biosynthesis genes in Burkholderia strains and characterized new genes involved in phenazine modification. The research found a connection between phenazine production and biofilm formation but did not link it to the pathogenicity of Burkholderia towards fruit flies and rot onions.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Forestry
Zhonghua Tu, Ziyuan Hao, Weiping Zhong, Huogen Li
Article
Plant Sciences
Ziyuan Hao, Xi Wang, Yaxian Zong, Shaoying Wen, Yanli Cheng, Huogen Li
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2019)
Article
Plant Sciences
ChengGe Zhang, HuanHuan Liu, YaXian Zong, ZhongHua Tu, HuoGen Li
Summary: In this study, the LtuGGPPS2 gene and its promoter were cloned from L. tulipifera, showing high homology to GGPPS genes of other plants and likely involvement in carotenoid and chlorophyll synthesis. Expression was highest in petals, and GUS staining indicated responsiveness to hormones. Overexpression increased carotenoid content and GGPPS enzyme activity, suggesting LtuGGPPS2 as a key terpenoid synthase in L. tulipifera flowers.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Lichun Yang, Huanhuan Liu, Ziyuan Hao, Yaxian Zong, Hui Xia, Yufang Shen, Huogen Li
Summary: The MYB transcription factor family, particularly R2R3-MYB genes, play a crucial role in pigment synthesis that determines petal colors in Liriodendron plants. Genome-wide identification and analysis revealed the presence of 204 LcMYB superfamily genes in Liriodendron chinense, with 85 R2R3-MYB genes distributed across 19 chromosomes. The expression profiles of four R2R3-MYB genes at a specific stage suggest their potential involvement in carotenoid synthesis in Liriodendron tulipifera petals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Lingmin Wei, Shaoying Wen, Zhonghua Tu, Yanqing Zhao, Huogen Li
Summary: This study investigates the role of the LtuJAG gene in leaf polarity formation by transferring it from Liriodendron tulipifera into Arabidopsis thaliana. The results show that LtuJAG acts as a negative regulator and affects leaf morphology and endogenous hormone levels in A. thaliana.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Z. Tu, L. Yu, S. Wen, X. Zhai, W. Li, H. Li
Summary: This study identified 36 LcHD-Zip genes in Liriodendron chinense and found that they play key roles in leaf development. These genes exhibit different expression patterns and are involved in leaf initiation, polarity establishment, shape development, phytohormone-mediated growth, and epidermal structure formation.
Article
Plant Sciences
Shaoying Wen, Zhonghua Tu, Lingming Wei, Huogen Li
Summary: The LcMAX1 gene in Liriodendron chinense is involved in root and shoot development, similar to the function of AtMAX1 in Arabidopsis thaliana. LcMAX1 promotes primary root growth by regulating auxin transport-related genes, while inhibiting shoot branching by upregulating the expression of AtBRC1 in the max1 mutant.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Genetics & Heredity
Hongying Li, Mengdi Chen, Zhengbo Wang, Ziyuan Hao, Xiping Zhao, Wenyan Zhu, Longchang Liu, Wei Guo
Summary: We extracted genomic DNA from Euonymus microcarpus and generated its chloroplast genome using high-throughput sequencing and de novo assembly. The chloroplast genome contains multiple genes and repetitive sequences and provides a valuable reference for genetic diversity and evolution studies.
Article
Plant Sciences
Hui Xia, Ziyuan Hao, Yufang Shen, Zhonghua Tu, Lichun Yang, Yaxian Zong, Huogen Li
Summary: In this study, GWAS analysis identified multiple significant loci associated with growth trajectories in hybrid Liriodendron populations. These loci showed temporal variations in effect sizes and inheritance patterns potentially related to different growth and development stages. The identified loci can be beneficial for selecting superior genotypes and provide a foundation for future molecular-assisted breeding.
Article
Forestry
Hongying Li, Zhengbo Wang, Yanwu Gao, Mengdi Chen, Ziyuan Hao, Xi Chen, Lili Guo, Chunjie Fan, Shutang Zhao
Summary: This study identified the AGO gene family in poplar and analyzed its tissue-specific expression and response to abiotic stress. A total of 15 PtAGO genes were found, mainly located in the nucleus and chloroplast. The PtAGO genes were clustered into 3 groups with different exon numbers and contained cis-regulatory elements involved in various biological processes. Tissue-specific expression patterns and stress-responsive nature were observed in PtAGO genes, providing insights for understanding their mechanisms and potential applications in poplar molecular breeding.