Journal
MICROMACHINES
Volume 10, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/mi10110750
Keywords
extracellular vesicles (EVs); cytochalasin B; mesenchymal stem cells (MSCs); nucleic acid delivery; nanovesicles; freezing and thawing
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Funding
- Russian Foundation for Basic Research [17-04-01136]
- Russian State funded budget project of ICBFM SB RAS [AAAA-A17-117020210024-8]
- Federal Research Center Institute of Cytology and Genetics SB RAS [0342-2019-0042]
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Extracellular vesicles provide cell-to-cell communication and have great potential for use as therapeutic carriers. This study was aimed at the development of an extracellular vesicle-based system for nucleic acid delivery. Three types of nanovesicles were assayed as oligonucleotide carriers: Mesenchymal stem cell-derived extracellular vesicles and mimics prepared either by cell treatment with cytochalasin B or by vesicle generation from plasma membrane. Nanovesicles were loaded with a DNA oligonucleotide by freezing/thawing, sonication, or permeabilization with saponin. Oligonucleotide delivery was assayed using HEK293 cells. Extracellular vesicles and mimics were characterized by a similar oligonucleotide loading level but different efficiency of oligonucleotide delivery. Cytochalasin-B-inducible nanovesicles exhibited the highest level of oligonucleotide accumulation in HEK293 cells and a loading capacity of 0.44 +/- 0.05 pmol/mu g. The loaded oligonucleotide was mostly protected from nuclease action.
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