Article
Immunology
Sergi Masgrau-Alsina, Lou Martha Wackerbarth, Dae-sik Lim, Markus Sperandio
Summary: This study identifies MST1 as a critical regulator of neutrophil homeostasis and mobilization from the bone marrow, shedding light on its complex role in regulating innate immunity.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Kellie A. Mouchemore, Robin L. Anderson
Summary: G-CSF has been reported to play a pro-tumour role in cancer by mainly affecting neutrophils and MDSCs, suppressing T cells in the presence of G-CSF. Recent studies have also highlighted other tumour-promoting effects of G-CSF on myeloid cells, raising concerns about its impact on anti-cancer treatments like immune checkpoint blockade.
SEMINARS IN IMMUNOLOGY
(2021)
Article
Medicine, General & Internal
Andrea Murru, Marie-eve Allard, Guillaume Pare, Myriam Vaillancourt, Lucie Boyer, Marie-Pierre Cayer, Julien Vitry, Patricia Landry, Marie-Michele Labrecque, Nancy Robitaille, Donald R. Branch, Melissa Girard, Maria J. Fernandes
Summary: This study compared the functional differences of neutrophils in prednisone and G-CSF GCs prepared by leukapheresis. The results showed that G-CSF GCs contained a higher number of neutrophils, but 40% of them were immature. Prednisone GC neutrophils showed enhanced phagocytosis compared to healthy donors, while G-CSF GC neutrophils exhibited decreased chemotaxis but increased IL-8 production. Leukapheresis and storage had significant impacts on the function of prednisone and G-CSF GC neutrophils.
FRONTIERS IN MEDICINE
(2022)
Review
Immunology
Hrishikesh M. Mehta, Seth J. Corey
Summary: A significant amount of continuous proliferation and differentiation is needed in adults to generate a billion new neutrophils daily, with G-CSF playing a crucial role in controlling granulopoiesis. CSF3R mutations are linked to various human disorders related to neutrophil production, including severe congenital neutropenia and myeloid malignancies. Despite more than thirty years since the discovery and characterization of G-CSF and G-CSF receptor, there are still fundamental questions about their physiological functions that remain unanswered.
SEMINARS IN IMMUNOLOGY
(2021)
Article
Hematology
Abdulrahman Theyab, Mohammad Algahtani, Khalaf F. Alsharif, Yousef M. Hawsawi, Abdulaziz Alghamdi, Adel Alghamdi, Jude Akinwale
Summary: G-CSF has drawn researchers' attention as a therapeutic agent for neutropenia patients, but its daily injections pose inconvenience. Understanding its structure, expression, and mechanism is crucial for more effective treatment.
Article
Immunology
Rongxin Dai, Ge Lv, Wenyan Li, Wenjing Tang, Junjie Chen, Qiao Liu, Lu Yang, Min Zhang, Zhirui Tian, Lina Zhou, Xin Yan, Yating Wang, Yuan Ding, Yunfei An, Zhiyong Zhang, Xuemei Tang, Xiaodong Zhao
Summary: SCN4 is an autosomal recessive disease caused by mutations in the G6PC3 gene, leading to various symptoms in patients. Neutrophil apoptosis is increased, while respiratory burst and extracellular traps production are impaired. G-CSF treatment is insufficient, suggesting regular treatment and long-term infection prevention as optimal methods for cure.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hiroshi Katayama
Summary: The acquired immunity against SARS-CoV-2 in convalescent COVID-19 patients, particularly the role of Th17 cells and the potential therapeutic effects of G-CSF, has been studied and may hold promise for future clinical trials.
Article
Immunology
Izabela Sieminska, Kazimierz Weglarczyk, Marcin Surmiak, Dorota Kurowska-Baran, Marek Sanak, Maciej Siedlar, Jarek Baran
Summary: The study found that even in COVID-19 convalescents with mild or asymptomatic infection, the levels of LDNs/PMN-MDSCs remained elevated in the blood 3 months after infection, negatively correlating with CD8(+) and double-negative T cells. Additionally, these cells were found to affect the production of anti-SARS-CoV-2 S1 neutralizing antibodies.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Katharina M. Glaser, Teresa K. Tarrant, Tim Laemmermann
Summary: G-protein coupled receptor kinases (GRKs) participate in the regulation of chemokine receptors, but they also have other functions beyond GPCR desensitization in primary immune cells. The interplay between different GRK family members and their effects on different immune cell types and GPCRs are complex and unpredictable. This study demonstrates the importance of studying GRK functions in primary immune cells to understand their specific roles.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Anna Peters, Philipp Rabe, Aenne-Dorothea Liebing, Petra Krumbholz, Anders Nordstrom, Elisabeth Jager, Robert Kraft, Claudia Staeubert
Summary: This study reveals the distinct signaling pathways of GPR84 and HCA(3) in immune cells. GPR84 promotes pro-inflammatory signaling in macrophages, while HCA(3) inhibits glycolytic metabolism and induces anti-inflammatory responses.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Cell Biology
Kohei Kemuriyama, Jianbo An, Satoru Motoyama, Yushi Nagaki, Tomokazu Yamaguchi, Yusuke Sato, Akiyuki Wakita, Yoshihiro Minamiya, Keiji Kuba
Summary: In this study, the researchers found that tumor-derived G-CSF promotes disease progression in various types of cancers. High expression of G-CSF in esophageal SCC (ESCC) tumor tissues is associated with poor prognosis. Deletion of G-CSF in tumor cells mitigated tumor growth and metastasis in mice. Mechanistically, G-CSF enhances cell proliferation in vitro and its accumulation in mice leads to an expansion of neutrophils and a decrease in CD8(+) T cells. Antibody depletion of neutrophils partially suppresses tumor growth but has no effect on distant metastasis. The findings suggest that G-CSF produced by tumor cells facilitates tumor progression through promoting neutrophil recruitment and tumor cell proliferation.
Review
Neurosciences
Yuka Ishikawa, Tomoyuki Furuyashiki
Summary: Stress-induced immune responses play important roles in depressive and anxiety-like behaviors in rodents. These findings may be relevant to the association between depression and brain and peripheral inflammations observed in clinical studies.
NEUROSCIENCE RESEARCH
(2022)
Article
Neurosciences
Mai Sakai, Zhiqian Yu, Ryo Hirayama, Masa Nakasato, Yoshie Kikuchi, Chiaki Ono, Hiroshi Komatsu, Miharu Nakanishi, Hatsumi Yoshii, David Stellwagen, Tomoyuki Furuyashiki, Masaaki Komatsu, Hiroaki Tomita
Summary: This study explores the changes in autophagy signaling in the prefrontal cortex (PFC) under repeated social defeat (RSD) and their potential involvement in behavioral changes induced by stress. The results suggest that enhanced autophagy may alleviate stress-induced depression, and microglial autophagy plays a role in stress-induced behavioral changes.
Letter
Cardiac & Cardiovascular Systems
Takuo Emoto, Hiroyuki Yamamoto, Tomoya Yamashita, Tomofumi Takaya, Takahiro Sawada, Shintaro Takeda, Masayuki Taniguchi, Naoto Sasaki, Naofumi Yoshida, Yoshihiro Saito, Tharini Sivasubramaniyam, Hiromasa Otake, Tomoyuki Furuyashiki, Clinton S. Robbins, Hiroya Kawai, Ken-ichi Hirata
Article
Biochemical Research Methods
Yusuke Kawashima, Hirotaka Nagai, Ryo Konno, Masaki Ishikawa, Daisuke Nakajima, Hironori Sato, Ren Nakamura, Tomoyuki Furuyashiki, Osamu Ohara
Summary: This paper discusses the demand for high proteome coverage in single-shot measurements and focuses on data-independent acquisition (DIA)-MS and ion mobility spectrometry as techniques for deep proteome analysis. The researchers aimed to expand the proteome coverage by optimizing high-field asymmetric waveform ion mobility spectrometry parameters in DIA-MS. The approach was applied to the analysis of host proteins in mouse feces and proved to be useful in understanding mental illness pathologies.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Multidisciplinary Sciences
Namiko Kawamura, Goro Katsuura, Nobuko Yamada-Goto, Riho Nakama, Yuki Kambe, Atsuro Miyata, Tomoyuki Furuyashiki, Shuh Narumiya, Yoshihiro Ogawa, Akio Inui
Summary: Fractalkine-CX3CR1 signaling induces anorexigenic actions via activation of the BDNF-TrkB pathway and suppresses HFD-induced hypothalamic inflammation in mice.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Satoshi Akiyama, Hirotaka Nagai, Shota Oike, Io Horikawa, Masakazu Shinohara, Yabin Lu, Takashi Futamura, Ryota Shinohara, Shiho Kitaoka, Tomoyuki Furuyashiki
Summary: This study found that chronic social stress increases the amount of lipid mediators related to 12S-LOX activity in the nucleus accumbens of mice. The increase is greater in stress-resilient mice, suggesting a correlation with stress resilience. These findings suggest that chronic social stress leads to a late increase in the amounts of 12S-LOX metabolites derived from brain vasculature in the nucleus accumbens.
SCIENTIFIC REPORTS
(2022)
Article
Neurosciences
Zhiqian Yu, Mai Sakai, Hotaka Fukushima, Chiaki Ono, Yoshie Kikuchi, Ryuta Koyama, Ko Matsui, Tomoyuki Furuyashiki, Satoshi Kida, Hiroaki Tomita
Summary: This study characterized the transcription profile of microglia in a fear memory conditional mouse model, and found that synapse-related genes were induced while immune-related genes were reduced after fear memory consolidation. The expression of synapse-related genes reversed after fear memory extinction, but immune-related genes remained unchanged. The expression of neurotransmitter release regulators in hippocampal microglia was also found to be correlated with fear memory formation and extinction.
BRAIN RESEARCH BULLETIN
(2022)
Article
Multidisciplinary Sciences
Zhiqian Yu, Mai Sakai, Hotaka Fukushima, Chiaki Ono, Yoshie Kikuchi, Ryuta Koyama, Ko Matsui, Tomoyuki Furuyashiki, Satoshi Kida, Hiroaki Tomita
Summary: This study investigated the gene transcription changes in microglia and peripheral monocytes after contextual fear conditioning in C57BL/6 J mice using Illumina MouseWG-6v2 microarrays. The findings are valuable for researchers interested in glial cells and neurotransmission studies.
Article
Pharmacology & Pharmacy
Hisayoshi Kubota, Kazuo Kunisawa, Bolati Wulaer, Masaya Hasegawa, Hitomi Kurahashi, Takatoshi Sakata, Hiroyuki Tezuka, Masanori Kugita, Shizuko Nagao, Taku Nagai, Tomoyuki Furuyashiki, Shuh Narumiya, Kuniaki Saito, Toshitaka Nabeshima, Akihiro Mouri
Summary: High salt intake is associated with hypertension and cognitive impairment. The angiotensin II (Ang II)-AT(1) receptor and prostaglandin E2 (PGE2)-EP1 receptor systems are involved in hypertension and neurotoxicity, but their role in high salt-induced hypertension and emotional and cognitive impairments is unclear. This study found that high salt intake leads to hypertension and cognitive impairments, potentially due to increased tau phosphorylation, decreased phosphorylation of Ca2+/calmodulin-dependent protein kinase II (CaMKII), and reduced expression of postsynaptic density protein 95 (PSD95) in the prefrontal cortex (PFC) and hippocampus (HIP). These changes can be blocked by pharmacological treatment with an AT(1) receptor blocker or EP1 receptor gene knockout.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mai Sakai, Zhiqian Yu, Masayuki Taniguchi, Rosanne Picotin, Nanami Oyama, David Stellwagen, Chiaki Ono, Yoshie Kikuchi, Ko Matsui, Miharu Nakanishi, Hatsumi Yoshii, Tomoyuki Furuyashiki, Takaaki Abe, Hiroaki Tomita
Summary: N-acetylcysteine (NAC) acts as an antioxidant and prevents cell death induced by tumor necrosis factor (TNF)-alpha, but it can also promote cell death through reactive oxygen species. This study investigated the effects of NAC on microglia and stress-induced behavior abnormalities in mice. The findings suggest that NAC has both beneficial and deleterious effects, with inhibitory effects on TNF-alpha and nitric oxide synthesis but high concentrations causing cell death in microglia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Rei Mishima, Masayuki Taniguchi, Kazutoshi Matsushita, Bowen Tian, Tomoyuki Furuyashiki
Summary: Using single-cell RNA sequencing, distinct microglial subpopulations with different transcriptome signatures were identified in the resting brain. The distribution of these subpopulations varied across brain regions, particularly between the cerebral cortices and the hypothalamus. Lipopolysaccharide and chronic social defeat stress, both involving the innate immune receptor TLR4, upregulated marker genes of selective microglial subpopulations. These findings highlight the contribution of microglial subpopulations to the heterogeneity of microglial transcriptome and responsiveness in different brain regions.
JOURNAL OF PHARMACOLOGICAL SCIENCES
(2023)
Article
Neurosciences
Nanami Kasakura, Yuka Murata, Asuka Shindo, Shiho Kitaoka, Tomoyuki Furuyashiki, Kanzo Suzuki, Eri Segi-Nishida
Summary: The dentate gyrus of the hippocampus plays a regulatory role in stress-related emotional behaviors and neurogenesis. NT-3 is expressed in the adult dentate gyrus and is enhanced under chronic stress conditions in rodents. However, the functional modulation of the dentate gyrus by NT-3 signaling is still unclear.
FRONTIERS IN NEUROSCIENCE
(2023)
Article
Cell Biology
Shiho Kitaoka, Ayaka Tomohiro, Shinya Ukeshima, Keyue Liu, Hidenori Wake, Shinya H. Kimura, Yasuhiko Yamamoto, Masahiro Nishibori, Tomoyuki Furuyashiki
Summary: Inflammation is associated with depression, and HMGB1 plays an important role in chronic stress-induced depression-related behaviors. HMGB1 can affect depression-related behaviors by regulating the Toll-like receptor (TLR) 2/4 in the medial prefrontal cortex (mPFC). The study found that HMGB1 has an antidepressive effect on social avoidance behavior, but the role of endogenous HMGB1 under chronic stress is still unknown.
Meeting Abstract
Neurosciences
Masayuki Taniguchi, Kazutoshi Matsushita, Rei Mishima, Mitsutaka Kadota, Shigehiro Kuraku, Tomoyuki Furuyashiki
NEUROPSYCHOPHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Risa Sakate, Masahiro Nishiyama, Yu Fukuda, Shiho Kitaoka, Tomoyuki Furuyashiki
Summary: The study reveals that Hhex negatively regulates the expression of inflammation-related genes in microglia, and activation of TLR2/4 decreases Hhex expression, thereby facilitating TLR4-mediated neuroinflammation.
JOURNAL OF PHARMACOLOGICAL SCIENCES
(2022)