Article
Plant Sciences
Stephen P. Slocombe, Tatiana Zuniga-Burgos, Lili Chu, Payam Mehrshahi, Matthew P. Davey, Alison G. Smith, Miller Alonso Camargo-Valero, Alison Baker
Summary: Remediation of phosphate pollution using micro-algae can be enhanced by pre-conditioning the algae to induce luxury phosphorus uptake. Over-expression of the global regulator PSR1 in algae led to increased P levels and accelerated removal of phosphate from the medium. The study also revealed a feed-forward mechanism involving P-transporter genes for hyper-accumulation of P even in the presence of excess external phosphate. These findings support a bio-circular approach of nutrient recycling from wastewater back to agriculture.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Environmental Sciences
Aigars Lavrinovics, Linda Mezule, Pavels Cacivkins, Talis Juhna
Summary: This study investigated the optimal initial conditions for the growth, phosphate removal, polyphosphate accumulation, and protein productivity of Chlorella vulgaris. Results showed that a 10-day period of phosphate deficiency, accompanied by low initial biomass concentration, significantly increased phosphate removal, polyphosphate accumulation, and protein productivity. The optimization model suggested that one-day biomass P-starvation with low initial biomass concentration was the most effective combination. These findings provide insights into using microalgae exposure to phosphorus stress for rapid phosphorus removal in wastewater treatment.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Microbiology
Aigars Lavrinovics, Fredrika Murby, Elina Ziverte, Linda Mezule, Talis Juhna
Summary: The study found that Chlorella vulgaris showed significant improvement in biomass growth rate and phosphorus removal rate under phosphorus starvation conditions, while Botryococcus braunii maintained good biomass growth and nutrient removal capabilities. All species exhibited a significant increase in alkaline phosphatase activity in phosphorus-starved biomass.
Article
Cell Biology
Tatiana Yu. Plyusnina, Sergei S. Khruschev, Polina V. Fursova, Alexei E. Solovchenko, Taras K. Antal, Galina Yu. Riznichenko, Andrei B. Rubin
Summary: The study investigated the dynamics of phosphate metabolism in Chlorella vulgaris during phosphate shortage and replenishment using a mathematical simulation approach. The model proposed two scenarios for the appearance of PolyP peaks in the cells, shedding light on the regulation of PolyP synthesis and hydrolysis. By analyzing the distribution of phosphorous within cell pools, the study contributed to a better understanding of phenomena during phosphate starvation and re-feeding in microalgal cultures.
Article
Engineering, Environmental
Nolan J. T. Pearce, Chris T. Parsons, Sarah M. Pomfret, Adam G. Yates
Summary: Effective modeling and management of phosphorus (P) losses from landscapes to waterbodies requires understanding of P retention and remobilization along the terrestrial-aquatic continuum. Stream periphyton has the capability to transiently store bioavailable P, but its response to dynamic P concentrations is largely unknown.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Tatsunori Hiyoshi, Kenta Oyanagi, Takuma Niki, Shoko Fujiwara, Norihiro Sato
Summary: This study focuses on the role of polyphosphate metabolism in cyanobacterial cells adapting to phosphorus-starved conditions, highlighting the importance of exopolyphosphatase in degrading polyphosphate. Additionally, the induction of ppx expression in response to phosphorus starvation through the two-component system, SphS and SphR, aids in cellular acclimation to phosphorus fluctuation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Environmental Sciences
Qirui Wu, Liang Guo, Yu Wang, Yangguo Zhao, Chunji Jin, Mengchun Gao, Zonglian She
Summary: Phosphorus (P) uptake, distribution, and transformation are crucial processes for the growth and metabolism of microalgae. Mixotrophic cultivation of microalgae results in higher biomass yield, with different trophic modes affecting the transformation trends of P among different substances.
Article
Plant Sciences
Aicha Loudari, Asmae Mayane, Youssef Zeroual, Gilles Colinet, Abdallah Oukarroum
Summary: Under salt stress conditions, adequate phosphorus fertilization can significantly improve wheat plant's photosynthetic efficiency, nutrient uptake, and biomass.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Mareike Kavka, Andrzej Majcherczyk, Ursula Kues, Andrea Polle
Summary: This study investigated the transcription patterns of PAPs in poplar under P shortage, identifying distinct P responsive expression patterns in roots and leaves for 23 PAPs. Activities of root-surface PAPs were found to increase significantly under low P availability, and proteomic analyses revealed three newly secreted proteins under P shortage, suggesting a central role of PtPAP1 in enhanced P acquisition from organic sources.
Article
Biotechnology & Applied Microbiology
M. Plouviez, P. Bolot, A. Shilton, B. Guieysse
Summary: The study found that dissolved P concentration positively influenced cellular P content, while biomass concentration and P-depletion time did not. Biomass concentration and P-depletion time positively influenced P uptake rates. Light supply neither influenced cellular P content nor P assimilation.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Biology
Yutaro Oishi, Rie Otaki, Yukari Iijima, Eri Kumagai, Motohide Aoki, Mikio Tsuzuki, Shoko Fujiwara, Norihiro Sato
Summary: Oishi et al. discovered that a green alga Chlorella kessleri synthesized a betaine lipid DGTS to replace phosphatidylcholine and phosphatidylethanolamine during phosphate deprivation. This study sheds light on the phosphorus-deficiency acclimation strategies in lower green plants through lipid remodeling.
COMMUNICATIONS BIOLOGY
(2022)
Editorial Material
Plant Sciences
Peng Wang, Erik Limpens, Ruifeng Yao
Summary: A recent study by Shi et al. reveals a transcriptional regulatory network for arbuscular mycorrhizal symbiosis in rice. The study highlights the importance of PHR transcription factors in coordinating the pathways for inorganic phosphate uptake, opening up opportunities for plant breeding to enhance nutrient acquisition.
TRENDS IN PLANT SCIENCE
(2022)
Article
Microbiology
Ravi Chawla, Steven Klupt, Vadim Patsalo, James R. Williamson, Lisa R. Racki
Summary: The protein AlgP is found to be associated with polyP granules and is required for their even spacing. AlgP binds to the chromosome and inhibits fusion between polyP granules, resulting in their evenly spaced distribution. This discovery provides insights into the functional significance of granule organization during starvation.
Article
Engineering, Environmental
Faqih Shuhaili, Mariona Segura-Noguera, Sivasudha Mathumathy, Rashmi Vijayaraghavan, Sivasudha Thilagar, Uma Lakshmanan, Dharmar Prabaharan, Seetharaman Vaidyanathan
Summary: This study systematically examined the nitrate and phosphate uptake response of two halotolerant strains of Chlorella vulgaris under different carbon, nitrogen, and phosphorus supply regimes. It was found that changes in carbon supply significantly influenced the uptake of nitrogen and phosphorus by the two strains. This investigation provides clear evidence for the role of supply regimes in nutrient uptake management in microalgae, as well as the significance of carbon supply in nitrogen and phosphorus uptake.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Agricultural Engineering
Renata Nunes Pereira, Debora Pez Jaeschke, Ligia Damasceno Ferreira Marczak, Rosane Rech, Giovana Domeneghini Mercali
Summary: This study evaluated the effects of ultrasound on the growth of Pseudoneochloris marina and Chlorella zofingiensis. The results showed that ultrasound treatment did not increase cell proliferation of P. marina, but reduced cell density, indicating possible cell damage. On the other hand, ultrasound treatment for C. zofingiensis resulted in a 65% increase in biomass concentration compared to the control. Overall, the study demonstrates the potential of ultrasound to enhance microalgae cell proliferation.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
S. G. Vasilieva, L. R. Semenova, I. O. Selyakh, O. B. Chivkunova, P. N. Shcherbakov, O. I. Baulina, O. A. Gorelova, E. S. Lobakova
Summary: This study investigated the immobilization of a mixed culture of microalgae and cyanobacteria on a chitosan-based polymer. The results showed that the chitosan sorbent had a high sorption capacity and provided stable cellular attachment for the immobilized culture. The immobilization on the chitosan sorbent increased the bioremoval of nitrates and phosphates by the mixed culture.
APPLIED BIOCHEMISTRY AND MICROBIOLOGY
(2022)
Article
Environmental Sciences
Ram Chandra Shrestha, Lusine Ghazaryan, Ben Poodiack, Boris Zorin, Avner Gross, Osnat Gillor, Inna Khozin-Goldberg, Ilya Gelfand
Summary: The study found that fertilization with algal biomass reduced wheat grain nitrogen content and soil nitrogen oxide emissions, leading to lower greenhouse gas emissions and global warming impact compared to urea fertilization.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Plant Sciences
Yogesh Taparia, Achintya Kumar Dolui, Sammy Boussiba, Inna Khozin-Goldberg
Summary: This study describes the construction of a modular two-component transcriptional unit system to express SpCas9 in a diatom. The system enables multiplexed targeting and marker-free genome editing. The editing efficiency of the system was evaluated by targeting the StLDP gene, and the results showed the importance of StLDP in lipid droplet regulation.
FRONTIERS IN PLANT SCIENCE
(2022)
Letter
Biochemical Research Methods
Mikhail Goncharov, Dmitry Bagaev, Dmitrii Shcherbinin, Ivan Zvyagin, Dmitry Bolotin, Paul G. Thomas, Anastasia A. Minervina, Mikhail V. Pogorelyy, Kristin Ladell, James E. McLaren, David A. Price, Thi H. O. Nguyen, Louise C. Rowntree, E. Bridie Clemens, Katherine Kedzierska, Garry Dolton, Cristina Rafael Rius, Andrew Sewell, Jerome Samir, Fabio Luciani, Ksenia V. Zornikova, Alexandra A. Khmelevskaya, Saveliy A. Sheetikov, Grigory A. Efimov, Dmitry Chudakov, Mikhail Shugay
Article
Biotechnology & Applied Microbiology
Giulia Trentin, Francesca Piazza, Marta Carletti, Boris Zorin, Inna Khozin-Goldberg, Alberto Bertucco, Eleonora Sforza
Summary: Two diazotrophic cyanobacteria were cultivated to produce cyanophycin under nitrogen fixing conditions. Nostoc sp. showed higher efficiency in accumulating cyanophycin and fixing atmospheric nitrogen. The operating conditions were optimized, with nitrogen availability and/or pH playing a major role in biomass production and phosphorus limitation maximizing cyanophycin accumulation.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Food Science & Technology
Ekaterina Novichkova, Sagar Nayak, Sammy Boussiba, Jacob Gopas, Dina Zilberg, Inna Khozin-Goldberg
Summary: The study investigates the anti-inflammatory and immunomodulatory potential of the microalga Lobosphaera incisa P127 in the zebrafish model of IBD. Results show that diets containing 15% non-starved (Ns) and 7.5% and 15% nitrogen-starved (St) algal biomass effectively attenuate gut injury, while diets containing 7.5% Ns and DGLA ethyl ester have no effect on gut condition.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Biology
Anastasia O. Smirnova, Anna M. Miroshnichenkova, Yulia Olshanskaya, Michael A. Maschan, Yuri B. Lebedev, Dmitriy M. Chudakov, Ilgar Z. Mamedov, Alexander Komkov
Summary: High-throughput sequencing of adaptive immune receptor repertoires is valuable for studying adaptive immunity, but accurately detecting and correcting clone frequency discrepancies remains challenging. This study identified an anomaly in the ratio between read count and clone count-based frequencies of non-functional clonotypes in multiplex PCR-based immune repertoires. They developed a quantitative measure called Over Amplification Rate (OAR) to evaluate and correct V and J gene frequency bias caused by multiplex PCR. The iROAR software was successfully tested and shown to increase the accuracy and consistency of repertoire reconstruction for comparative analysis.
Article
Chemistry, Multidisciplinary
Rostislav A. Filippov, Alexei Solovchenko, Dmitry Khort, Igor G. Smirnov, Alexey I. Kutyrev, Roman V. Pobedonostsev, Denis V. Yanykin
Summary: Electroimpedance spectroscopy technology can accelerate the healing of trees and assess plant condition after grafting. This study introduces a compact device that overcomes limitations of equipment complexity and measurement conditions. Cold atmospheric plasma (CAP) and plasma-treated solution (PTS) were used to treat pear grafts, promoting graft fusion and improving healing process. Impedance values showed better healing in plants treated with CAP and PTS compared to the control, indicating higher quality planting material can be obtained earlier than conventional methods.
APPLIED SCIENCES-BASEL
(2023)
Article
Biology
Tatiana A. Kozlova, Alexander V. Kartashov, Elena Zadneprovskaya, Anastasia Krapivina, Peter Zaytsev, Olga B. Chivkunova, Alexei E. Solovchenko
Summary: This study evaluated the influence of the phytohormone ABA on the physiology of Chlorella zofingiensis. The results showed that exogenous ABA stimulated cell division, biomass production, and biosynthesis of chlorophyll, carotenoids, and lipids. ABA also induced changes in the microbiome composition, increasing the abundance of Rhodococcus genus. The study discusses the potential applications of exogenous ABA for enhancing biomass, carotenoid, and fatty acid productivity in C. zofingiensis cultures.
Article
Chemistry, Multidisciplinary
Svetlana P. Chebotaryova, Olga Zakharova, Alexander A. Gusev, Petr A. Baranchikov, Evgenii A. Kolesnikov, Anastasia S. Yakusheva, Elena Skripnikova, Elena S. Lobakova, Jingliang Xu, Md Asraful Alam, Alexei E. Solovchenko
Summary: The widespread use of CuO nanoparticles (CuO-NP) for various purposes poses increasing environmental hazards to aquatic ecosystems. This study investigated the accumulation ability of the microalga Desmodesmus sp. for CuO-NP and their components, as well as its tolerance to environmentally relevant concentrations of CuO-NP. The results showed that Desmodesmus sp. is relatively tolerant to CuO-NP and has the potential for bioremoval of copper-based nanostructured hazardous micropollutants (HMP) from aquatic environments.
Article
Biochemistry & Molecular Biology
Olga V. Britanova, Kseniia R. Lupyr, Dmitry B. Staroverov, Irina A. Shagina, Alexey A. Aleksandrov, Yakov Y. Ustyugov, Dmitry V. Somov, Alesia Klimenko, Nadejda A. Shostak, Ivan V. Zvyagin, Alexey V. Stepanov, Ekaterina M. Merzlyak, Alexey N. Davydov, Mark Izraelson, Evgeniy S. Egorov, Ekaterina A. Bogdanova, Anna K. Vladimirova, Pavel A. Iakovlev, Denis A. Fedorenko, Roman A. Ivanov, Veronika I. Skvortsova, Sergey Lukyanov, Dmitry M. Chudakov
Summary: This article introduces a therapy for treating autoimmune diseases by selectively depleting specific T cell populations. The research was conducted in patients with ankylosing spondylitis and achieved successful outcomes.
Editorial Material
Plant Sciences
Zhi-Yan Du, Wajid Waheed Bhat, Guoyin Kai, Inna Khozin-Goldberg, Xiao-Hong Yu, Agnieszka Zienkiewicz, Krzysztof Zienkiewicz
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Horticulture
Andrei I. Kuzin, Natalia Ya. Kashirskaya, Alexei E. Solovchenko, Alexei V. Kushner, Anna M. Kochkina, Ludmila V. Stepantzova, Vyacheslav N. Krasin
Summary: The foliar application of micronutrients can improve nutrient uptake, reduce fertilizer usage, and maintain soil health. In an apple orchard experiment, manganese treatments increased foliar nitrogen content and zinc treatments enhanced foliar potassium. Low-rate chemical fertilizers application with initial high-rate organic fertilization allowed for optimal soil pH in the orchard.
Article
Nanoscience & Nanotechnology
P. A. Zaytsev, A. Kuzin, B. M. Shurygin, E. V. Skripnikova, S. A. Karpukhina, A. A. Zaytseva, A. E. Solovchenko
Summary: The biodiversity and stability of soil microbiome are crucial for preserving soil fertility in agroecosystems. Bio-based formulations that enhance soil biota activity and increase the bioavailability of mineral nutrients are becoming more widespread. DNA metabarcoding is an effective approach to assess the impact of agricultural practices on soil microbiome structure.
NANOBIOTECHNOLOGY REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Svetlana Vasilieva, Elena Lobakova, Olga Gorelova, Olga Baulina, Pavel Scherbakov, Olga Chivkunova, Larisa Semenova, Irina Selyakh, Alexandr Lukyanov, Alexei Solovchenko
Summary: The green microalga Lobosphaera has been found to exhibit high tolerance to elevated phosphate concentrations by converting it into polyphosphate. The cell structure also undergoes adjustments in response to phosphate levels.
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Sung-Yeoul Kim, Vikash Chandra Roy, Jin-Seok Park, Byung-Soo Chun
Summary: This study aims to extract functional substances from Undaria pinnatiida sporophyll using supercritical carbon dioxide (SC-CO2) and subcritical water extraction (SWE). The results showed that the consecutive green extraction method can yield extracts rich in fucoxanthin and beta-carotene, and the sporophyll of Undaria pinnatiida contains health-beneficial fatty acids. Additionally, SWE can extract substances with antioxidant, antibacterial, and antihypertensive activities.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2024)