Journal
TOXINS
Volume 11, Issue 10, Pages -Publisher
MDPI
DOI: 10.3390/toxins11100609
Keywords
laccase; mycotoxins; aflatoxin B-1; zearalenone; mediator; detoxification
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Funding
- Central Public-interest Scientific Institution Basal Research Fund of China [Y2019XK03]
- National Natural Science Foundation of China [31672458]
- National Key R&D Program of China [2016YFD050140902]
- Chinese Academy of Agricultural Science and Technology Innovation Project [CAAS-XTCX2016011]
- National Chicken Industry Technology System of China [CARS-41]
- Elite Youth Program of Chinese Academy of Agricultural Sciences
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Aflatoxin B-1 (AFB(1)) and zearalenone (ZEN) exert deleterious effects to human and animal health. In this study, the ability of a CotA laccase from Bacillus subtilis (BsCotA) to degrade these two mycotoxins was first investigated. Among the nine structurally defined chemical compounds, methyl syringate was the most efficient mediator assisting BsCotA to degrade AFB(1) (98.0%) and ZEN (100.0%). BsCotA could also use plant extracts, including the Epimedium brevicornu, Cucumis sativus L., Lavandula angustifolia, and Schizonepeta tenuifolia extracts to degrade AFB(1) and ZEN. Using hydra and BLYES as indicators, it was demonstrated that the degraded products of AFB(1) and ZEN using the laccase/mediator systems were detoxified. Finally, a laccase of fungal origin was also able to degrade AFB(1) and ZEN in the presence of the discovered mediators. The findings shed light on the possibility of using laccases and a mediator, particularly a natural plant-derived complex mediator, to simultaneously degrade AFB(1) and ZEN contaminants in food and feed.
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