Article
Engineering, Environmental
Zuotao Zhang, Jiao Sun, Xiaoqiang Gong, Chongyang Wang, Hui Wang
Summary: The synergistic metabolism between anammox cultures and nitrate-reducers for anaerobic PAH biodegradation is explored in this study. It includes the identification of anammox culture and anammox bacterium that can perform nitrogen metabolism in the biodegradation processes, the inhibitory effect of PAH and nitrite on the activities of anammox culture and nitrate-reducers, and the synergistic metabolic processes. The results show that anammox culture containing Candidatus Kuenenia efficiently reduces nitrite accumulation and inorganic carbon emissions, and significantly promotes biodegradation efficiency.
Article
Engineering, Environmental
Zuotao Zhang, Jiao Sun, Xiaoqiang Gong, Zhuoyue Yang, Chongyang Wang, Hui Wang
Summary: This study discovered a new pure culture, PheN4, which can anaerobically biodegrade phenanthrene using nitrate as the electron acceptor under different salinities. The optimal salinity for phenanthrene biodegradation by PheN4 is 5%. PheN4 can utilize benzene, naphthalene, and anthracene as carbon sources, but not other polycyclic aromatic hydrocarbons.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Environmental Sciences
Jiao Sun, Zuotao Zhang, Hui Wang, Matthew J. Rogers, Haijiao Guo, Jianzhong He
Summary: The study demonstrated the effective anaerobic degradation of PAHs using the anaerobic co-culture DYM1 obtained from petroleum-polluted soil without the need for additional terminal electron acceptors. This provides a novel approach for recovering energy from toxic pollutants in the form of methane.
ENVIRONMENTAL POLLUTION
(2022)
Article
Environmental Sciences
Zuotao Zhang, Haijiao Guo, Jiao Sun, Xiaoqiang Gong, Chongyang Wang, Hui Wang
Summary: This study identified a new sulfate-reducing phenanthrene-degrading bacterium strain PheS1 and proposed its biotransformation pathway under sulfate-reducing conditions. This discovery can serve as a reference for practical applications in bioremediation in anaerobic zones and for studying the molecular mechanisms of phenanthrene.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biochemistry & Molecular Biology
Zhimao Mai, Lin Wang, Qiqi Li, Yingting Sun, Si Zhang
Summary: Gordonia sp. SCSIO19801 is a bacterium isolated from the South China Sea that can effectively utilize phenanthrene as the sole carbon source. It degrades phenanthrene via the salicylate metabolic pathway and has the ability to utilize other aromatic compounds. These characteristics make it a potential candidate for biodegradation of petroleum hydrocarbons in marine environments.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Engineering, Environmental
Zuotao Zhang, Jiao Sun, Haijiao Guo, Xiaoqiang Gong, Chongyang Wang, Hui Wang
Summary: This study provides the first evidence of a pure-culture anaerobic phenanthrene-degrading sulfate-reducer, revealing the initial steps of anaerobic phenanthrene biodegradation processes with sulfate as an electron acceptor.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Review
Biotechnology & Applied Microbiology
Mayada Jamal, Annoor Awadasseid, Xiaomei Su
Summary: Anthraquinone dyes, widely used in textile manufacturing, pose a significant pollution threat due to their complex and stable structures. Microbiological treatment of wastewater has emerged as an economically viable solution, with promising advances in the biodegradation of anthraquinone dyes.
BIOTECHNOLOGY LETTERS
(2022)
Article
Engineering, Environmental
Shanshan Sun, Haizhen Wang, Kang Yan, Jun Lou, Jiahui Ding, Shane A. Snyder, Laosheng Wu, Jianming Xu
Summary: The co-culture of Rhodococcus sp. WB9 and Mycobacterium sp. WY10 effectively degraded phenanthrene, with WY10 playing a predominant role in the degradation process. This co-culture demonstrated metabolic cross-feeding between the two strains, where 1-hydroxy-2-naphthoic acid produced by WB9 served as a substrate for the growth of WY10, accelerating phenanthrene degradation. The synergistic degradation observed in this bacterial co-culture has potential for bioremediation applications.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Agricultural Engineering
Swati Sharma, Lalit M. Pandey
Summary: This study investigated the kinetic ability of pure and mixed cultures in the biodegradation of crude oil, and proposed an integrated kinetic model to explain the degradation process. The study found that the mixed cultures showed synergistic effects on the substrates, leading to increased degradation efficiency. Furthermore, the study found that the degradation efficiency was correlated with the activity of specific enzymes.
BIORESOURCE TECHNOLOGY
(2022)
Article
Engineering, Environmental
Kai Wang, Yang Sun, Dongyun Chen, Qingfeng Xu, Najun Li, Hua Li, Jianmei Lu
Summary: Bioaugmentation is a promising technology for removing polycyclic aromatic hydrocarbons, but high-efficiency microbial agents are limited. This study developed iron-modified biochar materials with green solvent polyethylene glycol 200 and Fe(NO3)3·9H2O through one-step pyrolysis. The modified biochar showed high microbial immobilization efficiency and improved surface structure. By coupling the effective adsorption of green materials with the biodegradability of microorganisms, the iron-modified biochar microorganism spheres exhibited efficient removal of phenanthrene from aqueous solution and soil.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Agricultural Engineering
Xiaolu Li, Yin Liu, Xuyao Li, Zhiyi Qin, Yu Su, Stefano Freguia, Leiyu Feng, Yinguang Chen
Summary: This prospective study investigated the influence of phenanthrene (PHE), a general polycyclic aromatic hydrocarbon found in waste activated sludge, on sludge alkaline dark fermentation for hydrogen accumulation. The yield of hydrogen increased by 1.3-fold compared to the control when PHE concentration was 50 mg/kg TSS, reaching 16.2 mL/g TSS. Mechanism research revealed that PHE promoted hydrogen production and the abundance of functional microorganisms, while reducing homoacetogenesis.
BIORESOURCE TECHNOLOGY
(2023)
Article
Microbiology
Ziyan Zhou, Yanqin Wang, Mingxia Wang, Zhifeng Zhou
Summary: The study enriched a microbial consortium capable of degrading phenanthrene under methanogenesis from paddy soil. Glucose addition increased the production rate of CH4 but did not affect phenanthrene degradation. The abundance of genes related to aromatic compound degradation decreased with glucose addition, while genes related to CH4 synthesis were unaffected. Additionally, the acetate pathway was found to be dominant in the phenanthrene-degrading system, indicating the potential for a multistage pattern to enhance PAH removal and CH4 production in anaerobic digestion.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biology
Jose Martin Marquez-Villa, Juan Carlos Rodriguez-Sierra, Nayem Amtanus Chequer, Nubia Noemi Cob-Calan, Jose Quinatzin Garcia-Maldonado, Santiago Cadena, Emanuel Hernandez-Nunez
Summary: Microbial degradation of aromatic hydrocarbons is a promising technology due to its economic efficiency and safety. However, the study of cyanobacteria-bacterial mutualistic interactions in this field is still limited. This research evaluated the phenanthrene biodegradation capacity of a consortium dominated by Fischerella sp. and identified the microbial diversity involved. The consortium was able to degrade up to 92% of phenanthrene in five days, with Fischerella sp. as the dominant strain and other bacteria also potentially involved in the degradation process.
Article
Biotechnology & Applied Microbiology
Alba Lara-Moreno, Francisco Merchan, Esmeralda Morillo, Jessica Zampolli, Patrizia Di Gennaro, Jaime Villaverde
Summary: Stenotrophomonas indicatrix CPHE1, isolated from contaminated soil, degrades PHE through dioxygenase, monooxygenase, and dehydrogenase genes. Different techniques, including biostimulation, nutrient solution, bioaugmentation, and the use of HPBCD, improve PHE mineralization in artificially contaminated soils.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Environmental Sciences
Xiaofang Shen, Yuyu Wan, Weihong Dong, Yujie Wei, Tong Li
Summary: Polycyclic aromatic hydrocarbons (PAHs) like naphthalene and phenanthrene are organic pollutants with toxic, carcinogenic, and teratogenic properties. Biodegradation is an effective method for their remediation. Indigenous microorganisms in riparian zones can remove PAHs, with naphthalene weakly promoting phenanthrene biodegradation. Factors such as initial concentration ratio, hydraulic gradient, and temperature influence the biodegradation process.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Biotechnology & Applied Microbiology
Yonghao Cui, Jianzhong He, Kun-Lin Yang, Kang Zhou
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2020)
Article
Engineering, Environmental
Siyan Zhao, Matthew J. Rogers, Jianzhong He
Article
Engineering, Environmental
Chang Ding, Matthew J. Rogers, Jianzhong He
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2020)
Article
Microbiology
Zuotao Zhang, Jiao Sun, Haijiao Guo, Chongyang Wang, Tingting Fang, Matthew J. Rogers, Jianzhong He, Hui Wang
Summary: This study investigated the anaerobic biotransformation of PAHs by a newly isolated phenanthrene-degrading denitrifier strain PheN1. The findings provide insights into the key steps of phenanthrene biotransformation and can guide PAHs bioremediation in anaerobic environments.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Review
Engineering, Environmental
Guofang Xu, Xuejie Zhao, Siyan Zhao, Chen Chen, Matthew J. Rogers, Rajaganesan Ramaswamy, Jianzhong He
Summary: Halogenated flame retardants (HFRs) are widely used in consumer products and are classified as persistent organic pollutants due to their resistance to degradation, bioaccumulation potential, and toxicity. This review provides an overview of the occurrence, fate, and impacts of HFRs in wastewater treatment plants (WWTPs) globally, including trends in distribution, removal mechanisms, transformation pathways, and potential impacts on reactor performance and microbiome in bioreactors.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Engineering, Environmental
Jing Yuan, Shuyao Li, Jie Cheng, Chenxi Guo, Chaofeng Shen, Jianzhong He, Yi Yang, Peijun Hu, Jianming Xu, Yan He
Summary: Previous studies often attribute microbial reductive dechlorination to organohalide-respiring bacteria (OHRB) or cometabolic dechlorination bacteria (CORB). This study found that methanogenesis is synchronized with OCP dechlorination, methanogens are important for maintaining microenvironment functional balance, and Methanosarcina barkeri (M. barkeri) can promote the dechlorination of gamma-hexachlorocyclohexane (gamma-HCH).
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Siyan Zhao, Matthew J. Rogers, Lifeng Cao, Chang Ding, Jianzhong He
Summary: PBDEs are persistent and highly toxic environmental pollutants. In this study, a novel Dehalococcoides mccartyi strain TZ50 was isolated from a PBDE-debrominating enrichment culture, showing the ability to debrominate PBDEs and dechlorinate PCE. The strain expresses RDases that mediate organohalide respiration of both PBDEs and chloroethenes, providing potential for remediation of cocontaminated sites.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Siyan Zhao, Chen Zhang, Matthew J. Rogers, Xuejie Zhao, Jianzhong He
Summary: This study developed a computational approach to identify genetic markers for discriminating between different strains of Dehalococcoides bacteria. By screening core genes from the Dehalococcoides pangenome, a gene encoding a bacterial neuraminidase repeat-containing protein was identified as a potential genetic marker. Using this marker, the researchers were able to effectively differentiate between different BNR sequences and Dehalococcoides isolates. The study also revealed a high diversity of Dehalococcoides in the environment.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Engineering, Environmental
Guofang Xu, Ning Zhang, Xuejie Zhao, Chen Chen, Chunfang Zhang, Jianzhong He
Summary: This study explores the potential for offshore marine microbiota to transform organohalide pollutants and finds that marine Dehalococcoidia have a versatile dehalogenation capacity. The study demonstrates dechlorination of diverse organohalides by offshore marine microbiota, including polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), tetrabromobisphenol A (TBBPA), and 2,4,6-trichlorophenol (2,4,6-TCP). It also identifies the presence of Dehalococcoides and uncultivated Dehalococcoidia in initial sediment microcosms, with Dehalococcoides being dominant in all enrichment cultures.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Engineering, Environmental
Guofang Xu, Hung Liang Ng, Chen Chen, Siyan Zhao, Jianzhong He
Summary: This study demonstrates that bioaugmentation with Dehalococcoides can accelerate the remediation of pentabrominated diphenyl ethers (PBDEs) in sediments, and the presence of tetrachloroethene (PCE) can improve the bioaugmentation performance. Ecological analysis shows that bioaugmentation leads to more deterministic microbial communities.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Engineering, Environmental
Guofang Xu, Siyan Zhao, Chen Chen, Xuejie Zhao, Rajaganesan Ramaswamy, Jianzhong He
Summary: This study reports the reductive dehalogenation of penta-BDEs and PCBs by Dehalococcoides mccartyi strain MB. The strain was able to efficiently debrominate penta-BDEs and dechlorinate PCBs through reductive dehalogenation. The research also revealed the involvement of MbrA in the dehalogenation process and explored the mechanism through proteomic profiling and transcriptional analysis.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Ecology
Siyan Zhao, Chang Ding, Guofang Xu, Matthew J. Rogers, Rajaganesan Ramaswamy, Jianzhong He
Summary: Anaerobic PBDE debromination was observed in microcosms established from e-waste recycling sites. Bacterial genera Dehalococcoides, Dehalogenimonas, and Dehalobacter were implicated in PBDE debromination. Complete debromination of penta-BDE mixture was also observed in axenic cultures of Dehalococcoides mccartyi strains. Four reductive dehalogenases were identified as potential markers for PBDE debromination in microbial communities.
Article
Ecology
Guofang Xu, Xuejie Zhao, Siyan Zhao, Matthew J. Rogers, Jianzhong He
Summary: Organohalide pollutants are commonly found in coastal regions and pose threats to public health and ecosystems. This study investigates the effects of salinity on microbial dechlorination of PCE and PCBs in freshwater and marine sediments. The findings suggest that marine-derived microcosms exhibit higher halotolerance and a halotolerant dechlorinating culture was enriched. The study also reveals that salinity influences microbial community composition and dechlorination capabilities. These insights contribute to understanding the fate and bioremediation of organohalide pollutants in changing salinity environments.
Article
Engineering, Environmental
Guofang Xu, Siyan Zhao, Chen Chen, Ning Zhang, Jianzhong He
Summary: Contamination of groundwater by multiple chlorinated solvents is a common issue that poses a significant challenge for effective remediation. This study reveals that different cocontaminants have varying inhibitory effects on the dechlorination process, with chloroform showing the strongest inhibition. The inhibitory effects of cocontaminants can be mitigated by introducing specific bacterial strains, resulting in the concurrent detoxification of multiple chlorinated solvents. Furthermore, the presence of cocontaminants leads to significant changes in the composition, structure, and assembly of the dechlorinating microbiome.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Microbiology
Jinting Liu, Guofang Xu, Siyan Zhao, Jianzhong He
Summary: This study systematically investigated the short- and long-term influence of microplastics and nanoplastics on the methanogenic digestion of waste-activated sludge (WAS). It found that plastics can have both enhancing and inhibitory effects on methanogenesis, with the coexistence of multiple plastics showing synergistic suppression. The inhibition of methanogenesis could be attributed to the generation of reactive oxygen species. The study also revealed the resilience and functional redundancy of the digestion sludge microbiome, contributing to the recovery of methanogenesis during long-term operation.