Review
Biotechnology & Applied Microbiology
Victor Alfonso Solarte David, Viviana Raquel Guiza-Argueello, Martha L. Arango-Rodriguez, Claudia L. Sossa, Silvia M. Becerra-Bayona
Summary: The absence or damage of tissue is a major cause of diseases, and novel therapeutic alternatives aim to recover lost functions through tissue regeneration. Chronic cutaneous lesions are common wounds that require regenerative medicine and tissue engineering interventions to develop bioactive medical products for proper healing and prevention of complications. The coordination of cells, extracellular matrix (ECM), and biomolecules is crucial in tissue repair and regeneration, with ECM acting as a biological platform for their interactions. Tissue engineering utilizes synthetic polymers and decellularized tissues to mimic native ECM and provide necessary functionalities for cell differentiation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Immunology
Morten Asser Karsdal, Virginia Byers Kraus, Diane Shevell, Anne Christine Bay-Jensen, Joern Schattenberg, R. Rambabu Surabattula, Detlef Schuppan
Summary: Connective tissue remodeling is crucial in tissue regeneration, but imbalances can lead to chronic diseases. Blood-based assays for collagen fragments play a key role in quantifying and defining the nature of connective tissue remodeling, aiding in the early detection and non-invasive monitoring of various chronic and autoimmune diseases.
AUTOIMMUNITY REVIEWS
(2021)
Article
Engineering, Biomedical
Agustina Setiawati, Dongyoon Jang, Daeyon Cho, Shingyu Cho, Hayan Jeong, Sungmin Park, Jungsug Gwak, Soo Ryeon Ryu, Won Hee Jung, Bong-Gun Ju, Kwang-Hwan Jung, Oh-Sun Kwon, Kwanwoo Shin
Summary: The research introduces a new method to coat absorbable sutures with fibronectin, promoting cellular migration and adhesion while preventing infection. The modified suture also reduces friction with wounded tissue, enhancing overall healing outcomes.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Engineering, Biomedical
Chen Wang, Guoyun Li, Kaige Cui, Zihan Chai, Ziyu Huang, Yue Liu, Shang Chen, Haoyan Huang, Kaiyue Zhang, Zhibo Han, Yuhao Li, Guangli Yu, Zhong-Chao Han, Na Liu, Zongjin Li
Summary: In this study, the glycosaminoglycanomics of decellularized extracellular matrices (dECMs) from perinatal tissues were investigated, highlighting the critical roles of sulfated glycosaminoglycans (GAGs) in promoting tissue regeneration. The placenta-derived dECM hydrogel showed superior therapeutic effects due to higher contents of chondroitin sulfate with 6-O-sulfo group (CS-6S) and heparan sulfate with 6-O-sulfo group (HS-6S), which play important roles in inflammation and angiogenesis. This study provides insight into the use of placenta-derived ECM hydrogel for regenerative applications.
ACTA BIOMATERIALIA
(2021)
Article
Pharmacology & Pharmacy
Andrea De Pieri, Keegan Ocorr, Kyle Jerreld, Mikkael Lamoca, Wolfgang Hitzl, Karin Wuertz-Kozak
Summary: This study successfully encapsulated resveratrol into poly(epsilon-caprolactone) microparticles to prolong and protect its release and activity. These microparticles showed no cytotoxicity and stimulated collagen deposition, while reducing inflammation and proteolysis.
Review
Cell & Tissue Engineering
Koyeli Girigoswami, Devender Saini, Agnishwar Girigoswami
Summary: This article discusses the importance of stem cell growth and fate in therapy and regeneration, particularly their role in repairing damage. It also points out that the tumor microenvironment may affect the growth of stem cells, and details how uncontrolled cell growth in cancer changes the extracellular matrix (ECM).
STEM CELL REVIEWS AND REPORTS
(2021)
Review
Medicine, General & Internal
Simona Pompili, Giovanni Latella, Eugenio Gaudio, Roberta Sferra, Antonella Vetuschi
Summary: The intestinal extracellular matrix (ECM) is a complex network of proteins that plays a crucial role in maintaining tissue structure and function. Interaction between ECM and cells is essential for cell development, proliferation, and apoptosis.
FRONTIERS IN MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Janina Burk, Anna Sassmann, Cornelia Kasper, Ariane Nimptsch, Susanna Schubert
Summary: Matrix remodeling by MSC in ECM disease could be influenced by the ECM environment. MSC adapt to the ECM conditions, including pathological adaptations, which may affect therapeutic efficacy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Neurosciences
Bhanu P. P. Tewari, Lata Chaunsali, Courtney E. E. Prim, Harald Sontheimer
Summary: The extracellular matrix (ECM) and perineuronal nets (PNNs) serve as structural scaffolds in the central nervous system (CNS) with diverse functions, including neurogenesis, cell migration and differentiation, axonal growth, and synaptic stability. Glial cells play a key role in regulating the malleability of ECM and PNNs, impacting their functions in both health and diseases.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Review
Pharmacology & Pharmacy
Sumama Nuthana Kalva, Robin Augustine, Abdulla Al Mamun, Yogesh Bharat Dalvi, Namitha Vijay, Anwarul Hasan
Summary: Healing chronic diabetic ulcers, burn wounds, and large traumatic wounds is a significant clinical challenge, but submicron fiber-based membranes can mimic the natural extracellular matrix, promoting wound healing and tissue regeneration. Various active components can be incorporated into electrospun fibers to improve ECM mimetic features and provide additional advantages.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2021)
Article
Cell Biology
Luisa Strackeljan, Ewa Baczynska, Carla Cangalaya, David Baidoe-Ansah, Jakub Wlodarczyk, Rahul Kaushik, Alexander Dityatev
Summary: Microglia play a role in regulating the remodeling of perineuronal nets (PNNs) and perisynaptic extracellular matrix (ECM), as well as the fine structure of PNNs and excitatory terminals in the hippocampus.
Article
Cell Biology
Vinay Kumar Dubey, Souradeep R. Sarkar, Vairavan Lakshmanan, Rimple Dalmeida, Akash Gulyani, Dasaradhi Palakodeti
Summary: Extracellular matrix (ECM) plays a crucial role in stem cell function and tissue homeostasis. This study reveals the regulatory role of the transcription factor ETS-1 in the expression of ECM regulator mt-mmpA, which modulates basement membrane thickness and affects stem cell migration and epidermal progenitor distribution in planarians.
JOURNAL OF CELL SCIENCE
(2022)
Article
Engineering, Biomedical
Zahra Bashiri, Motahareh Rajabi Fomeshi, Hatef Ghasemi Hamidabadi, Davod Jafari, Sanaz Alizadeh, Maryam Nazm Bojnordi, Gorka Orive, Alireza Dolatshahi-Pirouz, Maria Zahiri, Rui L. Reis, Subhas C. Kundu, Mazaher Gholipourmalekabadi
Summary: Extracellular matrix (ECM)-based bioinks are widely studied for 3D printing of native-like tissue constructs. In this study, a printable bioink biomimicking natural skin was prepared using different concentrations of placental ECM, sodium-alginate, and gelatin. The 5% ECM 3D printed scaffold showed the best deep wound regeneration, producing a skin replacement with a cellular structure comparable to native skin.
MATERIALS TODAY BIO
(2023)
Review
Cell Biology
Xin Bi, Ye Li, Ziqing Dong, Jing Zhao, Weizi Wu, Jialiang Zou, Lingling Guo, Feng Lu, Jianhua Gao
Summary: Remodeling of the extracellular matrix (ECM) is vital for adipose tissue regeneration after autologous fat grafting, influencing factors diverse and not fully understood. Rapid and high-quality ECM remodeling can improve the retention rate after fat grafting.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Walker D. D. Short, Oluyinka O. O. Olutoye, Benjamin W. W. Padon, Umang M. M. Parikh, Daniel Colchado, Hima Vangapandu, Shayan Shams, Taiyun Chi, Jangwook P. P. Jung, Swathi Balaji
Summary: Impaired wound healing is a significant financial and medical burden. Disruptions in ECM deposition, structure, and composition lead to impaired healing in diseased states, such as in diabetes. Valid measures include lack of bacterial contamination, good tissue perfusion, and reduced mechanical injury and strain. This review discusses bioengineering advances in detecting biologic and physiologic factors, visualizing and modeling the ECM, and efficiently evaluating wound data. It focuses on bioelectronics and biologic interfaces for real-time sensing and actuation, advanced imaging techniques, and computational modeling. These advancements have the potential to improve healing outcomes and guide decision-making in wound care.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Letter
Dermatology
Signe Holm Nielsen, Solveig Skovlund Groen, Yiqiu Yao, Astrid-Helene Ravn Jorgensen, Valdemar Wendelboe Nielsen, Morten Karsdal, Kristoffer Gehring, Anne-Christine Bay-Jensen, Simon Francis Thomsen
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Editorial Material
Gastroenterology & Hepatology
Emilie K. Dahl, Osama K. Abed, Jens Kjeldsen, Marco Donia, Inge M. Svane, Anders Dige, Jorgen S. Agnholt, Jacob T. Bjerrum, Jakob B. Seidelin
ALIMENTARY PHARMACOLOGY & THERAPEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Helena Port, Anne-Christine Bay-Jensen, Yi He, Morten A. A. Karsdal, Thorbjorn Gantzel, Christian S. S. Thudium, Signe Holm Nielsen
Summary: The development of a competitive chemiluminescence enzyme-linked immunosorbent assay, CALC2, targeting the type II collagen C-terminal pro-peptide as a marker of cartilage formation was evaluated. The CALC2 level was measured after in vitro cleavage of recombinant type II collagen and treatment of human osteoarthritis cartilage explant model with insulin-like growth factor-1. Serum CALC2 levels were found to be decreased in patients with rheumatoid arthritis and ankylosing spondylitis compared to controls. These results suggest that CALC2 may be a novel biomarker of type II collagen formation, but further studies are needed to confirm these findings.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Gastroenterology & Hepatology
Jacob Broder Brodersen, Jens Kjeldsen, Torben Knudsen, Michael Dam Jensen
Summary: This study aims to compare the agreement between pan-enteric capsule endoscopy (CE) and ileocolonoscopy (IC) for determining the severity and classification of lesions in ileocolonic Crohn's disease (CD). The results show a strong correlation between IC and CE in assessing the severity of ileocolonic CD, but fair to moderate agreement in sub-scores.
ENDOSCOPY INTERNATIONAL OPEN
(2023)
Article
Hematology
Eline D. P. van Bergen, Simon C. Mastbergen, Floris P. J. G. Lafeber, Anne-Christine Bay-Jensen, Sofie F. Madsen, Helena Port, Wouter Foppen, Roger E. G. Schutgens, Mylene P. Jansen, Lize F. D. van Vulpen
Summary: This study aimed to quantitatively evaluate the structural changes after AJD in patients with HAA by using 3D joint space width (JSW) measurements and biochemical markers, and to correlate these findings with clinical pain/function. The results showed that on group level, there was a slight decrease in percentage changes in JSW after 12 months, followed by a non-statistically significant increase after 36 months compared to baseline. Biochemical marker collagen/cartilage formation also showed an initial decrease, followed by a trend towards net formation 12, 24 and 36 months after AJD. On individual patient level, no clear correlations between structural changes and clinical parameters were observed.
Article
Biochemistry & Molecular Biology
Christina Jensen, Patryk Drobinski, Jeppe Thorlacius-Ussing, Morten A. Karsdal, Anne-Christine Bay-Jensen, Nicholas Willumsen
Summary: This study found that cancer patients have lower levels of autoantibodies against denatured type III collagen in their serum compared to healthy individuals, suggesting a close relationship between autoimmunity and tumor control.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Stefania Kalogera, Mylene P. Jansen, Anne-Christine Bay-Jensen, Peder Frederiksen, Morten A. Karsdal, Christian S. Thudium, Simon C. Mastbergen
Summary: The association between structural changes and pain sensation in osteoarthritis (OA) is still not clear. This study found that some biomarkers were associated with structural outcomes, while most were associated with pain outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Medicine, General & Internal
Yunyun Luo, Martin Pehrsson, Lasse Langholm, Morten Karsdal, Anne-Christine Bay-Jensen, Shu Sun
Summary: Immunoassays have become increasingly important in clinical practice and biomedical research, but they are plagued by lot-to-lot variance, which negatively affects assay accuracy and precision. This article explores the reasons and locations of lot-to-lot variance, and proposes methods to mitigate its effects. The findings highlight the importance of considering lot-to-lot variance in assay development and application.
Article
Biochemistry & Molecular Biology
Federica Genovese, Peder Frederiksen, Anne-Christine Bay-Jensen, Morten A. Karsdal, Anna M. Milan, Birgitta Olsson, Mattias Rudebeck, James A. Gallagher, Lakshminarayan R. Ranganath
Summary: Nitisinone treatment affects biomarkers of joint tissue remodelling in AKU patients, providing insights into the disease pathogenesis and treatment effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nursing
Janni Petersen Fallesen, Marie Louise Krogh, Lisbeth Rosenbek Minet, Mette Maria Skjoth, Torben Knudsen, Jens Kjeldsen
Summary: This study aims to explore the communication experiences between patients with Inflammatory bowel disease and healthcare professionals in outpatient clinics, and to identify the important factors such as easy access, predictability of follow-up appointments, privacy, and quality of time with healthcare professionals.
JOURNAL OF CLINICAL NURSING
(2023)
Article
Gastroenterology & Hepatology
Jacob Broder Brodersen, Michael Dam Jensen, Mie Agerbaek Juel, Jens Kjeldsen, Torben Knudsen, Soren Rafael Rafaelsen
Summary: Trainee-performed intestinal ultrasound (IUS) showed improved diagnostic accuracy for detecting Crohn's disease (CD), especially in detecting small bowel and colon lesions. The diagnostic performance of trainees improved over time. There was no difference in diagnostic performance between IUS and expert-evaluated magnetic resonance enterocolonography (MREC) for detecting CD.
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
(2023)
Article
Gastroenterology & Hepatology
Anders Mark-Christensen, Line Riis Jolving, Pavithra Laxsen Anru, Joseph A. Murray, Rasmus Gaardskaer Nielsen, Niels Qvist, Soren Laurberg, Henriette Engberg, Jens Kjeldsen, Bente Mertz Norgard
Summary: In this nationwide Danish cohort study, it was found that patients with ulcerative colitis who underwent total colectomy had a higher risk of being diagnosed with inflammatory and autoimmune disease (IAD) compared to those who did not.
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
(2023)
Article
Gastroenterology & Hepatology
Sofia Hjerrild Thomsen, Pantea Zinolabedinbik, Jacob Broder Brodersen, Torben Knudsen, Jens Kjeldsen, Michael Dam Jensen
Summary: This study examined the additional information obtained with mucosal biopsies and the feasibility of CE as a single diagnostic procedure. The results showed that the additional information obtained from biopsies is limited in patients with a reliable CE result, and biopsies are still warranted for patients with an atypical endoscopic appearance or suspected malignancy.
ENDOSCOPY INTERNATIONAL OPEN
(2023)
Article
Rheumatology
Maja Skov Kragsnaes, Jesus Miguens Blanco, Benjamin H. Mullish, Jose Ivan Contreras-Serrano, Jens Kjeldsen, Hans Christian Horn, Jens Kristian Pedersen, Heidi Lausten Munk, Anna Christine Nilsson, Ash Salam, Matthew R. Lewis, Elena Chekmeneva, Karsten Kristiansen, Julian R. Marchesi, Torkell Ellingsen
Summary: This study investigated the intestinal permeability and metabolomic profiles in methotrexate-treated psoriatic arthritis patients, and their relationship with clinical response. The results showed that intestinal permeability and metabolomic profiles were associated with the primary clinical trial end point.
ACR OPEN RHEUMATOLOGY
(2023)
Article
Medicine, Research & Experimental
Ken Lund, Torben Knudsen, Jens Kjeldsen, Rasmus Gaardskaer Nielsen, Carsten Bogh Juhl, Bente Mertz Norgard
Summary: This study aims to investigate the effect of exercise intervention on moderately active IBD patients, evaluating its impact on disease-specific health-related quality of life, general health status, and biomarkers.
