Article
Environmental Sciences
Neda Gilannejad, Francisco J. Moyano, Gonzalo Martinez-Rodriguez, Manuel Yufera
Summary: Understanding the daily digestive function is crucial for efficient feeding practices in farmed fish species. Higher feeding frequencies, especially during dark hours, were found to be more suitable for aquaculture production of the Senegalese sole.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Joao Afonso, Cristina Guedes, Alfredo Teixeira, Paulo Rema, Severiano Silva
Summary: The ultrasound approach can accurately predict the fillet volume of Senegalese sole, showing promise for measuring in vivo fish composition traits. However, the results for fillet yield estimation were more modest, indicating the need for further studies to fully understand the potential of ultrasound in this trait.
Article
Fisheries
Diana Bastos Almeida, Catarina Magalhaes, Zelia Sousa, Maria Teresa Borges, Eliane Silva, Isidro Blanquet, Ana Paula Mucha
Summary: Recirculating aquaculture systems (RAS) manage waste and nutrient recycling to make intensive fish production environmentally sustainable. This study investigated the dynamics of prokaryotic communities in relation to water parameters, finding that shifts in the community were modeled by factors such as salinity and pH. The research provides new knowledge essential for understanding microbial community balance in aquaculture and serves as a valuable tool for overall aquaculture system management.
Article
Multidisciplinary Sciences
Israel Guerrero-Cozar, Jessica Gomez-Garrido, Concha Berbel, Juan F. Martinez-Blanch, Tyler Alioto, M. Gonzalo Claros, Pierre-Alexandre Gagnaire, Manuel Manchado
Summary: The integration of physical and high-density genetic maps in the assembly of the Senegalese sole genome at a chromosome-level scaffold revealed differences in genomic recombination rates between sexes and identified important sex-associated genetic loci. Analysis of sex markers showed incomplete penetrance with males as the heterogametic sex. Comparison with other Pleuronectiformes genomes also revealed chromosomal rearrangements, including a lineage-specific Robertsonian fusion in S. senegalensis.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Diogo Peixoto, Wilson Pinto, Ana Teresa Goncalves, Marina Machado, Bruno Reis, Joana Silva, Joao Navalho, Jorge Dias, Luis Conceicao, Benjamin Costas
Summary: The study evaluated the effects of dietary microalgal inclusion on the health status and growth performance of Senegalese sole post-larvae. Results showed that adding Nannochloropsis sp., Phaeodactylum sp., and Chlorella sp. to the diets may improve growth performance of Senegalese sole post-larvae without adverse effects on immune status.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Andre Barreto, Wilson Pinto, Andreia Rodrigues, Rui J. M. Rocha, Carlos Unamunzaga, Tome Silva, Jorge Dias, Luis E. C. Conceicao
Summary: The study indicates that broken cell biomass of P. tricornutum has a positive effect on the growth performance of Senegalese sole, but no significant impact on oxidative stress was observed.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Fisheries
Jose-Pedro Canavate, Victoria Anguis-Climent, Catalina Fernandez-Diaz
Summary: This study evaluates the nutritional contribution of the green seaweed Ulva ohnoi in the diet of Senegalese sole. The results show that the seaweed is a good source of proteins and lipids for the fish, with high carbon assimilation rates in muscle and liver. The study also suggests that lipid extraction has minimal impact on the results.
Article
Agriculture, Dairy & Animal Science
Marco Mendizabal-Castillero, Manuel Alejandro Merlo, Ismael Cross, Maria Esther Rodriguez, Laureana Rebordinos
Summary: In this study, hox gene clusters in the Senegalese sole were characterized to better understand the developmental and metamorphosis process in this species. Cytogenetic analysis revealed the localization of hox genes on specific chromosomes. Comparative and phylogenetic analysis showed conserved organization within each hox cluster, but different clustering patterns between clusters. Analysis of structural and repetitive sequences identified polymorphisms mediated by repetitive elements in the hoxba gene cluster, suggesting a possible loss of the hoxb7a gene in the Pleuronectiformes lineage. This work provides insights into the organization and regulation of hox clusters, and serves as a foundation for further studies on expression patterns.
Article
Fisheries
Lucia Vazquez-Salgado, Jose G. Olveira, Carlos P. Dopazo, Isabel Bandin
Summary: Intensive fish farming at high densities has negative effects on fish welfare, with NNV-infected Senegalese sole reared at high densities showing earlier mortality onset and higher viral load.
JOURNAL OF FISH DISEASES
(2021)
Article
Environmental Sciences
Diana Bastos Almeida, Miguel Semedo, Catarina Magalhaes, Isidro Blanquet, Ana Paula Mucha
Summary: This study aims to detect prokaryotic interactions in recirculating aquaculture systems (RAS) and found a mostly cooperative environment. Positive interactions were detected between ammonia oxidizing bacteria and archaea with nitrite oxidizing bacteria, but no interactions between them, suggesting niche differentiation based on ammonia competition. There was also a significant positive correlation between taxa associated with pathogenic outbreaks, indicating its relevance in disease preventions and outbreak predictions.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Ana Manuela de Azevedo, Ana Paula Losada, Isabel Ferreiro, Ana Riaza, Vanesa Losada, Tommaso Russo, Clara Boglione, Sonia Vazquez, Maria Isabel Quiroga
Summary: This study evaluated the presence of vertebral anomalies in postlarvae and juvenile Senegalese sole fed with Artemia spp. metanauplii enriched with four commercial products. The results showed a high percentage of individuals with skeletal anomalies in every dietary group, and some variations in the frequency of anomalies among diets were noted. However, it was not possible to establish a clear effect of the enrichment products on the development of vertebral deformities due to the masking effect of other rearing conditions.
Article
Marine & Freshwater Biology
Marcelino Herrera, Ana Rodiles, Natalia Salamanca, Jose Ignacio Navas, Maria Luisa Cordero, Ismael Hachero
Summary: This study aims to describe and analyze the results of the first flatfish stock enhancements in Andalusia. The results show that it is possible to release and recover tagged Senegal soles in the Gulf of Cadiz, and future long-term enhancement programs could help recover fisheries stocks.
Article
Environmental Sciences
Helena Oliveira, Ana Luisa Maulvault, Sara Castanho, Tiago Repolho, Luisa M. P. Valente, Pedro Pousao-Ferreira, Rui Rosa, Antonio Marques, Patricia Anacleto
Summary: Rising levels of atmospheric CO2 are causing ocean warming and acidification, which have potential negative effects on commercially important fish species. This study examined the effects of ocean acidification (OA) and warming (OW), both individually and combined, on the composition, fitness, and energy budget of juvenile Senegalese sole. The results showed that acidification and warming did not significantly impact the proximate composition and gross energy of the fish. However, OA, OW, and OAW treatments resulted in higher growth rates, metabolic rates, and energy intake compared to control conditions. The study highlights the physiological responses of juvenile Senegalese sole to ocean warming and acidification, emphasizing the importance of understanding the impact of climate change on fisheries.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Michael N. Viegas, Maria Antonia Salgado, Carla Aguiar, Agostinho Almeida, Paulo Gavaia, Jorge Dias
Summary: The study found that supplementing organic Mn and Zn to the diet of Senegalese sole post-larvae can improve survival rates, reduce vertebral malformations, and increase Mn deposition in bone.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2021)
Article
Multidisciplinary Sciences
Sandra Ramos-Judez, Wendy Angela Gonzalez-Lopez, Jhons Huayanay Ostos, Noemi Cota Mamani, Carlos Marrero Aleman, Jose Beirao, Neil Duncan
Summary: Injecting gonadotropin-releasing hormone agonist to induce ovulation in cultured female Senegalese sole, and collecting sperm from cultured males for in vitro fertilization can improve fertilization success rate. The viability of unfertilized eggs stored at room temperature gradually decreases, and faster fertilization results in higher egg viability.
ROYAL SOCIETY OPEN SCIENCE
(2021)
Article
Plant Sciences
Angeles Cuadrado, Marta Sixto, Rosa I. Figueroa, Isabel Bravo, Alfredo de Bustos
Summary: Through FISH analysis, we found differences in chromosomal organization and rDNA repeat unit numbers among different Gambierdiscus species. The location and organization of 5S and 45S rDNA in the nucleus also vary among species. These species can be distinguished by their species-specific FISH rDNA phenotypes.
EUROPEAN JOURNAL OF PHYCOLOGY
(2022)
Article
Genetics & Heredity
Abdulsamad Wafa, Belal Ali, Faten Moassass, Maged Kheder, Abdulmunim Aljapawe, Bassel Al-Halabi, Kristin Mrasek, Thomas Liehr, Walid Al-Achkar
Summary: This study investigated the CEBPA gene mutations in a Syrian family with AML. The results showed that all affected family members had a good response to chemotherapy and achieved complete remission. This research is important for understanding the relationship between CEBPA gene mutations and AML.
MOLECULAR GENETICS & GENOMIC MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Daniel Ramirez, Maria Esther Rodriguez, Ismael Cross, Alberto Arias-Perez, Manuel Alejandro Merlo, Marco Anaya, Silvia Portela-Bens, Paulino Martinez, Francisca Robles, Carmelo Ruiz-Rejon, Laureana Rebordinos
Summary: The chromosome evolution of Solea senegalensis, a commercially-important flatfish species, was studied using a cytogenomics approach and synteny analysis with two reference flatfish species. It was found that repetitive sequences played a role in the evolution of bi-armed and acrocentric chromosomes, with differences in distribution and abundance of repetitive elements suggesting their involvement in remodeling processes during evolution.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Roberto de la Herran, Miguel Hermida, Juan Andres Rubiolo, Jessica Gomez-Garrido, Fernando Cruz, Francisca Robles, Rafael Navajas-Perez, Andres Blanco, Paula Rodriguez Villamayor, Dorinda Torres, Pablo Sanchez-Quinteiro, Daniel Ramirez, Maria Esther Rodriguez, Alberto Arias-Perez, Ismael Cross, Neil Duncan, Teresa Martinez-Pena, Ana Riaza, Adrian Millan, M. Cristina De Rosa, Davide Pirolli, Marta Gut, Carmen Bouza, Diego Robledo, Laureana Rebordinos, Tyler Alioto, Carmelo Ruiz-Rejon, Paulino Martinez
Summary: This study reports a novel molecular sexing tool, which identified 41 single nucleotide polymorphism variants in the follicle stimulating hormone receptor (fshr) gene through whole genome resequencing of six males and six females, and validated the sex association. The study also found differential expression of the fshr gene between male and female gonads at an early stage of gonadal differentiation, suggesting a mechanism hampering the action of the follicle stimulating hormone driving the undifferentiated gonad toward testis.
MOLECULAR ECOLOGY RESOURCES
(2023)
Article
Agriculture, Dairy & Animal Science
Marco Mendizabal-Castillero, Manuel Alejandro Merlo, Ismael Cross, Maria Esther Rodriguez, Laureana Rebordinos
Summary: In this study, hox gene clusters in the Senegalese sole were characterized to better understand the developmental and metamorphosis process in this species. Cytogenetic analysis revealed the localization of hox genes on specific chromosomes. Comparative and phylogenetic analysis showed conserved organization within each hox cluster, but different clustering patterns between clusters. Analysis of structural and repetitive sequences identified polymorphisms mediated by repetitive elements in the hoxba gene cluster, suggesting a possible loss of the hoxb7a gene in the Pleuronectiformes lineage. This work provides insights into the organization and regulation of hox clusters, and serves as a foundation for further studies on expression patterns.
Article
Biochemistry & Molecular Biology
Gustavo A. Toma, Natalia dos Santos, Rodrigo dos Santos, Petr Rab, Rafael Kretschmer, Tariq Ezaz, Luiz A. C. Bertollo, Thomas Liehr, Fabio Porto-Foresti, Terumi Hatanaka, Alongklod Tanomtong, Ricardo Utsunomia, Marcelo B. Cioffi
Summary: The study investigated the karyotypes of different color phenotypes in Scleropages formosus using molecular cytogenetic techniques. The results revealed population genetic structure and microstructure differences in the karyotypes of the color phenotypes, but did not conclusively support the hypothesis of discrete lineages or evolutionary units among them.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Dagmar Heydeck, Christoph Ufer, Kumar R. Kakularam, Michael Rothe, Thomas Liehr, Philippe Poulain, Hartmut Kuhn
Summary: The researchers created transgenic mice expressing human ALOX15 under the control of the aP2 promoter, which showed high expression in adipocytes, bone marrow cells, and peritoneal macrophages. The catalytic activity of the transgenic enzyme was confirmed through activity assays and plasma metabolite analysis. The aP2-ALOX15 mice exhibited gender-specific differences in body-weight kinetics compared to the wildtype controls.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Entomology
Jhon A. D. Vidal, Francisco de M. C. Sassi, Renata L. R. de Moraes, Roberto F. Artoni, Thomas Liehr, Marcelo B. Cioffi, Mara C. de Almeida
Summary: In this study, the similarity of X and Y chromosomes in four Omophoita species was examined, and genomic differentiation was compared to better understand the evolutionary process of the giant sex chromosomes. The results showed significant genomic similarity between the sexes and a sex-specific region on the Y chromosome, as well as genomic divergence between species. However, whole chromosome painting experiments revealed high intra- and interspecific similarity of the sex chromosomes in O. octoguttata. Our data support a common origin and canonical evolution of the sex chromosomes in this group, as they have high genomic similarity between them.
Review
Biochemistry & Molecular Biology
Galina Hovhannisyan, Tigran Harutyunyan, Rouben Aroutiounian, Thomas Liehr
Summary: Cell-free DNA (cfDNA) has become a commonly used diagnostic marker in various pathologies, including viral infections like COVID-19, due to its higher sensitivity compared to standard biochemical parameters. This review focuses on the identification, sources, molecular features, and mechanisms of release and clearance of cfDNA. It discusses the general suitability of cfDNA for diagnostic purposes, potential limitations, and future directions, with a special emphasis on coronavirus infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Francisco de Menezes Cavalcante Sassi, Alexandr Sember, Geize Aparecida Deon, Thomas Liehr, Niklas Padutsch, Osvaldo Takeshi Oyakawa, Marcelo Ricardo Vicari, Luiz Antonio Carlos Bertollo, Orlando Moreira-Filho, Marcelo de Bello Cioffi
Summary: The study investigates the chromosomal rearrangements and associated synteny blocks in the origin of a multiple X1X2Y sex chromosome system in the Neotropical catfish genus Harttia. The results suggest that the X1X2Y sex chromosome systems in H. punctata, H. duriventris and H. villasboas represent the same linkage groups which also form the putative XY sex chromosomes of H. rondoni. The study also indicates that the X1X2Y sex chromosome system may have formed through an X chromosome fission rather than a previously proposed Y-autosome fusion.
SCIENTIFIC REPORTS
(2023)
Article
Entomology
Francisco Panzera, Angeles Cuadrado, Pablo Mora, Teresa Palomeque, Pedro Lorite, Sebastian Pita
Summary: This study analyzed the distribution of microsatellites in the holocentric chromosomes of Triatominae, which are vectors of Chagas disease. The results showed that there are differences in microsatellite distribution between Triatomini and Rhodniini species, with higher abundance and diversity in Triatomini. Additionally, a high abundance of GATA repeats was found in the Y chromosome of all Triatomini species, suggesting it is an ancestral trait specific to Triatomini.
Article
Agriculture, Dairy & Animal Science
Claudio A. Jimenez-Ruiz, Roberto de la Herran, Francisca Robles, Rafael Navajas-Perez, Ismael Cross, Laureana Rebordinos, Carmelo Ruiz-Rejon
Summary: MicroRNAs play crucial roles in regulating gene expression, and the miR-430 family is particularly important in early fish development. This study found that the miR-430 family emerged early in fish evolution, and all studied species have multiple copies of miR-430. In some early-diverged fish, three different versions of miR-430 were identified, but some groups seem to have lost some of these versions over time. These findings suggest the need for further research on the functions of different miR-430 versions and their potential impact on aquaculture.
Article
Biology
Mariannah Pravatti Barcellos de Oliveira, Rafael Kretschmer, Geize Aparecida Deon, Gustavo Akira Toma, Tariq Ezaz, Caio Augusto Gomes Goes, Fabio Porto-Foresti, Thomas Liehr, Ricardo Utsunomia, Marcelo de Bello Cioffi
Summary: In this study, the dynamics of satellite DNAs (SatDNAs) accumulation and elimination during W chromosome differentiation in Triportheus fish were investigated. The majority of repetitive sequences were eliminated as speciation occurred in five additional Triportheus species. While the W chromosomes continued to degenerate, the Z chromosomes in some species began to accumulate TauSatDNAs. Species-specific SatDNAs were likely amplified in the heterochromatic regions of both Z and W chromosomes in each species. Thus, the W chromosomes of various Triportheus species underwent significant evolutionary changes within a short period of time (15-25 Myr) after divergence.
Article
Biochemistry & Molecular Biology
Renata Luiza Rosa de Moraes, Francisco de Menezes Cavalcante Sassi, Jhon Alex Dziechciarz Vidal, Caio Augusto Gomes Goes, Rodrigo Zeni dos Santos, Jose Henrique Forte Stornioli, Fabio Porto-Foresti, Thomas Liehr, Ricardo Utsunomia, Marcelo de Bello Cioffi
Summary: Chromosomal rearrangements play a significant role in the evolution of fish genomes, and satellite DNA sequences have potential relevance in both sex and karyotype evolution. This study investigated the association of satDNAs with chromosome-shuffling events and their involvement in the evolution of sex chromosomes in Pyrrhulina fish. In addition, a large collection of satDNAs for both species was characterized for the first time, and their potential involvement in karyotype evolution was demonstrated.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Geize Aparecida Deon, Larissa Glugoski, Terumi Hatanaka, Francisco de Menezes Cavalcante Sassi, Viviane Nogaroto, Luiz Antonio Carlos Bertollo, Thomas Liehr, Ahmed Al-Rikabi, Orlando Moreira Filho, Marcelo de Bello Cioffi, Marcelo Ricardo Vicari
Summary: This study investigates the chromosomal rearrangements in the Neotropical armored catfish genus Harttia using powerful tools such as comparative whole chromosome painting. The results reveal the importance of translocation events in the karyotype evolution of Harttia species, as well as the independent origin of two types of multiple sex chromosomes.
GENETICS AND MOLECULAR BIOLOGY
(2022)
