RNA-based qPCR as a tool to quantify and to characterize dual-species biofilms

Unveiling the role of Gardnerella vaginalis in polymicrobial Bacterial Vaginosis biofilms: the impact of other vaginal pathogens living as neighbors

(2019) Joana Castro et al.   ISME Journal

Tetracycline and rifampicin induced a viable but nonculturable state in Staphylococcus epidermidis biofilms

(2018) Virginia Carvalhais et al.   Future Microbiology

Quantitative assessment of individual populations within polymicrobial biofilms

(2018) Susana Patrícia Lopes et al.   Scientific Reports

A comparison of methods used to unveil the genetic and metabolic pool in the built environment

(2018) Cinta Gomez-Silvan et al.   Microbiome

Developing a model for cystic fibrosis sociomicrobiology based on antibiotic and environmental stress

(2017) Susana Patrícia Lopes et al.   INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY

Staphylococcus aureus Impacts Pseudomonas aeruginosa Chronic Respiratory Disease in Murine Models

(2017) Cristina Cigana et al.   JOURNAL OF INFECTIOUS DISEASES

Effect of Histophilus somni on Heart and Brain Microvascular Endothelial Cells

(2017) D. O’Toole et al.   VETERINARY PATHOLOGY

Comparison of DNA-, PMA-, and RNA-based 16S rRNA Illumina sequencing for detection of live bacteria in water

(2017) Ru Li et al.   Scientific Reports

Insights into Cystic Fibrosis Polymicrobial Consortia: The Role of Species Interactions in Biofilm Development, Phenotype, and Response to In-Use Antibiotics

(2017) Andreia P. Magalhães et al.   Frontiers in Microbiology

Schrödinger’s microbes: Tools for distinguishing the living from the dead in microbial ecosystems

(2017) Joanne B. Emerson et al.   Microbiome

Polymicrobial Ventilator-Associated Pneumonia: Fighting In Vitro Candida albicans-Pseudomonas aeruginosa Biofilms with Antifungal-Antibacterial Combination Therapy

(2017) Maria E. Rodrigues et al.   PLoS One

Simultaneous and quantitative monitoring of co-cultured Pseudomonas aeruginosa and Staphylococcus aureus with antibiotics on a diffusometric platform

(2017) Chih-Yao Chung et al.   Scientific Reports

Targeting Staphylococcus aureus Toxins: A Potential form of Anti-Virulence Therapy

(2016) Cin Kong et al.   Toxins

Assessing and reducing sources of gene expression variability inStaphylococcus epidermidisbiofilms

(2016) Cármen Sousa et al.   BIOTECHNIQUES

Development of Real-Time PCR Methods for the Detection of Bacterial Meningitis Pathogens without DNA Extraction

(2016) Jeni Vuong et al.   PLoS One

Respiratory microbiota resistance and resilience to pulmonary exacerbation and subsequent antimicrobial intervention

(2015) Leah Cuthbertson et al.   ISME Journal

Quantification ofPseudomonas aeruginosain multispecies biofilms using PMA-qPCR

(2015) Sarah Tavernier et al.   PeerJ

Dynamics of Biofilm Formation and the Interaction between Candida albicans and Methicillin-Susceptible (MSSA) and -Resistant Staphylococcus aureus (MRSA)

(2015) Chaiene Evelin Zago et al.   PLoS One

Dormancy within Staphylococcus epidermidis biofilms: a transcriptomic analysis by RNA-seq

(2014) Virginia Carvalhais et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Species-specific viability analysis of Pseudomonas aeruginosa, Burkholderia cepacia and Staphylococcus aureus in mixed culture by flow cytometry

(2014) Marc Rüger et al.   BMC MICROBIOLOGY

Characterization and resuscitation of ‘non-culturable’ cells of Legionella pneumophila

(2014) Adrien Ducret et al.   BMC MICROBIOLOGY

Synergistic Interactions of Pseudomonas aeruginosa and Staphylococcus aureus in anIn VitroWound Model

(2014) Stephanie DeLeon et al.   INFECTION AND IMMUNITY

Evaluation of quantitative PCR combined with PMA treatment for molecular assessment of microbial water quality

(2014) Eva Theres Gensberger et al.   WATER RESEARCH

Modeling the Impact of Antibiotic Exposure on Human Microbiota

(2014) Jiangchao Zhao et al.   Scientific Reports

Biofilm Matrix and Its Regulation in Pseudomonas aeruginosa

(2013) Qing Wei et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Evaluating rRNA as an indicator of microbial activity in environmental communities: limitations and uses

(2013) Steven J Blazewicz et al.   ISME Journal

Antibiotic pressure can induce the viable but non-culturable state in Staphylococcus aureus growing in biofilms

(2013) S. Pasquaroli et al.   JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

Optimization of an automatic counting system for the quantification ofStaphylococcus epidermidiscells in biofilms

(2013) Ana Isabel Freitas et al.   JOURNAL OF BASIC MICROBIOLOGY

Culture-based diagnostic microbiology in cystic fibrosis: Can we simplify the complexity?

(2013) Jane L. Burns et al.   Journal of Cystic Fibrosis

Validation of a quantitative real-time PCR assay and comparison with fluorescence microscopy and selective agar plate counting for species-specific quantification of anin vitrosubgingival biofilm model

(2013) T. W. Ammann et al.   JOURNAL OF PERIODONTAL RESEARCH

Influence of anaerobic conditions on vaginal microbiota recovery from bacterial vaginosis patients

(2013) Tatiana B Cereija et al.   SEXUALLY TRANSMITTED INFECTIONS

Polymicrobial Interactions: Impact on Pathogenesis and Human Disease

(2012) B. M. Peters et al.   CLINICAL MICROBIOLOGY REVIEWS

Variability of RNA Quality Extracted from Biofilms of Foodborne Pathogens Using Different Kits Impacts mRNA Quantification by qPCR

(2012) Angela França et al.   CURRENT MICROBIOLOGY

A flow cytometric method for viability assessment ofStaphylococcus aureusandBurkholderia cepaciain mixed culture

(2012) Marc Rüger et al.   CYTOMETRY PART A

Optimizing a qPCR Gene Expression Quantification Assay for S. epidermidis Biofilms: A Comparison between Commercial Kits and a Customized Protocol

(2012) Angela França et al.   PLoS One

Long-term cultivation-independent microbial diversity analysis demonstrates that bacterial communities infecting the adult cystic fibrosis lung show stability and resilience

(2012) Franziska Anne Stressmann et al.   THORAX

Induction of Viable but Nonculturable Escherichia coli O157:H7 in the Phyllosphere of Lettuce: a Food Safety Risk Factor

(2011) Laura-Dorina Dinu et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Bacterial adaptation during chronic infection revealed by independent component analysis of transcriptomic data

(2011) Lei Yang et al.   BMC MICROBIOLOGY

Interaction of legionella pneumophila and helicobacter pylori with bacterial species isolated from drinking water biofilms

(2011) Maria S Gião et al.   BMC MICROBIOLOGY

Influence of copper ions on the viability and cytotoxicity of Pseudomonas aeruginosa under conditions relevant to drinking water environments

(2011) Zenyta Dwidjosiswojo et al.   INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH

Use of culture and molecular analysis to determine the effect of antibiotic treatment on microbial community diversity and abundance during exacerbation in patients with cystic fibrosis

(2011) M. M. Tunney et al.   THORAX

Substantial performance discrepancies among commercially available kits for reverse transcription quantitative polymerase chain reaction: A systematic comparative investigator-driven approach

(2010) Matthias W. Sieber et al.   ANALYTICAL BIOCHEMISTRY

Specific and Rapid Enumeration of Viable but Nonculturable and Viable-Culturable Gram-Negative Bacteria by Using Flow Cytometry

(2010) M. M. T. Khan et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Rhamnolipids: diversity of structures, microbial origins and roles

(2010) Ahmad Mohammad Abdel-Mawgoud et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

The exclusion of dead bacterial cells is essential for accurate molecular analysis of clinical samples

(2010) G.B. Rogers et al.   CLINICAL MICROBIOLOGY AND INFECTION

Reliability of Quantitative Real-Time PCR for Bacterial Detection in Cystic Fibrosis Airway Specimens

(2010) Edith T. Zemanick et al.   PLoS One

Transcription level of messenger RNA per gene copy determined with dual-spike-in strategy

(2009) Yi Zhang et al.   ANALYTICAL BIOCHEMISTRY

Molecular Detection of Viable Bacterial Pathogens in Water by Ratiometric Pre-rRNA Analysis

(2009) G. A. Cangelosi et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

(2009) S. A. Bustin et al.   CLINICAL CHEMISTRY

Degradation of 16S rRNA and attributes of viability of viable but nonculturable probiotic bacteria

(2008) S.J. Lahtinen et al.   LETTERS IN APPLIED MICROBIOLOGY

Physiological heterogeneity in biofilms

(2008) Philip S. Stewart et al.   NATURE REVIEWS MICROBIOLOGY

Molecular Detection of Multiple Emerging Pathogens in Sputa from Cystic Fibrosis Patients

(2008) Fadi Bittar et al.   PLoS One