Review
Health Care Sciences & Services
Yuuki Shichi, Fujiya Gomi, Norihiko Sasaki, Keisuke Nonaka, Tomio Arai, Toshiyuki Ishiwata
Summary: Pancreatic ductal adenocarcinoma (PDAC) is a difficult-to-diagnose and incurable cancer. Recent studies have found that PDAC cell lines can be divided into epithelial and mesenchymal cell lines, which show differences in morphology, function, and drug effects. Understanding the characteristics of these cell lines may contribute to the development of new anticancer drugs.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Sang-Hoon Yoon, Mi-Rae Bae, Hyeonwoo La, Hyuk Song, Kwonho Hong, Jeong-Tae Do
Summary: This study proposed an efficient method for deriving NSCs from mouse ESCs through a combination of 3D culture for early neural lineage commitment and 2D NSC derivation. The results demonstrated that ESCs could efficiently differentiate into tripotent NSCs through specification in 3D culture followed by 2D culture.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Multidisciplinary
Xinkai Cao, Ni Ren, Ganglu Tian, Yuxing Fan, Qingling Duan
Summary: A method for three-dimensional prediction of dissolved oxygen in pond water, based on Attention-GRU and GBRT, was proposed in this study. The results showed significant improvements in accurately predicting dissolved oxygen levels in different spatial areas of the pond compared to traditional one-dimensional methods.
COMPUTERS AND ELECTRONICS IN AGRICULTURE
(2021)
Review
Biochemistry & Molecular Biology
Eri Mukai, Shimpei Fujimoto, Nobuya Inagaki
Summary: The dysfunction of pancreatic beta-cells is crucial in the development of type 2 diabetes mellitus, characterized by impaired glucose stimulation and reduced ATP production. High glucose metabolism leads to the generation of reactive oxygen species in mitochondria, which affects beta-cells due to low expression of antioxidant enzymes. Activating the antioxidant defense factor Nrf2 may improve beta-cell dysfunction caused by ROS.
Article
Medicine, Research & Experimental
Junsuke Uwada, Shoichiro Mukai, Naoki Terada, Hitomi Nakazawa, Mohammad Sayful Islam, Takahiro Nagai, Masato Fujii, Koji Yamasaki, Takanobu Taniguchi, Toshiyuki Kamoto, Takashi Yazawa
Summary: In 3D-cultured prostate cancer cells, probenecid demonstrated pleiotropic effects such as chemosensitization, growth suppression, inhibition of spheroid compaction, and suppression of anchorage-independent growth. Elucidating the detailed mechanism underlying these probenecid actions could result in the identification of novel therapeutic targets toward the advanced prostate cancer.
Article
Engineering, Environmental
Wenting Yu, Xiaoxia Su, Moxiao Li, Wanting Wan, Ang Li, Hong Zhou, Feng Xu
Summary: This study investigated the effects of matrix strain on the characteristics and bioactivity of MSC-derived exosomes by culturing PDLSCs in three-dimensional magnetically stretched collagen hydrogels. The results showed that altering the miRNA content in exosomes significantly enhanced their bioactivity, leading to improved repair of bone defects in vivo. This research suggests a novel strategy for promoting tissue regeneration through engineered mechanical microenvironments.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Multidisciplinary Sciences
Huixia Ren, Yanjun Li, Chengsheng Han, Yi Yu, Bowen Shi, Xiaohong Peng, Shufang Wu, Xiaojing Yang, Sneppen Kim, Liangyi Chen, Chao Tang, Tianming Zhang
Summary: The Ca2+ modulated pulsatile glucagon and insulin secretions by pancreatic alpha and beta cells play a crucial role in glucose homeostasis. However, the coordination between alpha and beta cells in producing different Ca2+ oscillation patterns is still unclear. This study used a microfluidic device and transgenic mice to record Ca2+ signals from islet alpha and beta cells and observed heterogeneous Ca2+ oscillation patterns intrinsic to each islet. The results showed that after glucose stimulation, the oscillations of alpha and beta cells were globally phase-locked, with a fixed time delay in the activation of alpha cells compared to beta cells and a tunable period in the activation of beta cells. The number of islet alpha cells was also found to be correlated with oscillation frequency. A mathematical model incorporating paracrine interactions was built and quantitatively agreed with the experimental data, highlighting the importance of cell-cell interaction in generating stable but tunable islet oscillation patterns.
NATURE COMMUNICATIONS
(2022)
Article
Engineering, Marine
Gangqin Tu, Haifei Liu, Zhiming Ru, Wei Yang, Tao Sun, Liming Xing, Yu Ding
Summary: With the expansion of aquaculture, the environmental impact of suspended aquaculture is becoming increasingly important. This study used a three-dimensional lattice Boltzmann model to simulate the flow field and seston distribution in a net cage used for scallop fishing, and analyzed the influences of scallop number and current velocity on the local environment. The results showed that the number of scallops affected flow velocity and seston flux, and led to variable local seston consumption.
Article
Multidisciplinary Sciences
Habiba Alaa, Mariam Abdelaziz, Maryam Mustafa, Mustafa Mansour, Salma Magdy, Salma Mohsen, Yomna El-Karamany, Alyaa Farid
Summary: Nanotechnology is used to address issues in current pharmaceuticals, such as limited solubility and quick drug release. Melatonin has been shown to regulate glucose levels but its sensitivity to oxidation and poor oral bioavailability present challenges. The study aimed to synthesize melatonin loaded nanoparticles and assess their efficacy in treating diabetes in rats. The nanoparticles demonstrated anti-inflammatory, anti-coagulant, and anti-oxidant effects, reduced blood glucose levels, and promoted pancreatic cell regeneration.
SCIENTIFIC REPORTS
(2023)
Article
Nutrition & Dietetics
Wenqian Gu, Camilla Christine Bundgaard Anker, Christine Bodelund Christiansen, Tilo Moede, Per-Olof Berggren, Kjeld Hermansen, Soren Gregersen, Per Bendix Jeppesen
Summary: The study found that islet beta cells suppress glucagon secretion from alpha cells via direct cell-to-cell contact during co-culture, leading to a significant decrease in glucagon secretion from the alpha cells. However, this effect did not impact insulin secretion from beta cells, and the distribution of cells in co-culture differed from the in vivo arrangement of beta and alpha cells in mouse islets.
Article
Chemistry, Applied
Ji-Eun Jeong, Sang-Soo Han, Hye-Eun Shim, Woojin Kim, Byoung-Seok Lee, Yong- Jin Kim, Sun-Woong Kang
Summary: This study proposes a new technique for generating spheroids using scaffold-based methods, which successfully produced uniform-sized spheroids with high viability and increased expression levels of proteins and genes required for cell-specific functions. These physiologically relevant models have potential applications in regenerative medicine and drug screening.
CARBOHYDRATE POLYMERS
(2022)
Article
Biochemistry & Molecular Biology
Benson Koh, Nadiah Sulaiman, Mh Busra Fauzi, Jia Xian Law, Min Hwei Ng, Too Lih Yuan, Abdul Ghani Nur Azurah, Mohd Heikal Mohd Yunus, Ruszymah Bt Hj Idrus, Muhammad Dain Yazid
Summary: This study aimed to explore the potential of xeno-free three-dimensional cultures for mesenchymal stem cell (MSCs) expansion in clinical applications. Human serum and human platelet lysate were found to be suitable alternatives to replace foetal bovine serum in MSCs microcarrier cultures. Low glucose DMEM (LG) + human platelet (HPL) lysate was identified as the best xeno-free culture media, promoting high cell yield and maintaining MSC characteristics. This culture system showed improved immunomodulatory activities, suggesting its potential for future clinical applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Mireille M. J. P. E. Sthijns, Timo Rademakers, Jolien Oosterveer, Thomas Geuens, Clemens A. van Blitterswijk, Vanessa L. S. LaPointe
Summary: This study reveals that oxidative stress in pancreatic islet cells can be reduced by modulating the composition and dimensionality of co-cultures. Both aggregate culture and an increase in the prevalence of beta cells confer greater resistance to oxidative stress-induced cell death. Culturing alpha and beta cells in a 50:50 ratio in 3D aggregates prevents oxidative stress in both cell types.
Review
Biochemistry & Molecular Biology
Novia Hanasti, Lia Faridah, Azzania Fibriani, Hesti Lina Wiraswati, Diah Kusumawaty, Savira Ekawardhani
Summary: Cell culture is crucial in biological research, but 2D cell culture has limitations in accurately modeling in vivo conditions. 3D cell culture with added biomaterials has been explored as a more accurate method to mimic the cancer environment.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Biophysics
Patrycja Sokolowska, Kamil Zukowski, Justyna Janikiewicz, Elzbieta Jastrzebska, Agnieszka Dobrzyn, Zbigniew Brzozka
Summary: This study presents an Islet-on-a-chip system for three-dimensional pancreatic islet cell culture and analysis, utilizing micropillar-based microfluidic platform. The system ensures proper cell distribution, high viability, and optimal culture conditions for cell aggregates, which may be useful for testing pathogenic effectors and therapeutic agents in the future.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Engineering, Biomedical
Yanyi Xu, Zhenqing Li, Xiaofei Li, Zhaobo Fan, Zhenguo Liu, Xiaoyun Xie, Jianjun Guan
ACTA BIOMATERIALIA
(2015)
Article
Nanoscience & Nanotechnology
Zhenqing Li, Zhaobo Fan, Yanyi Xu, Wilson Lo, Xi Wang, Hong Niu, Xiaofei Li, Xiaoyun Xie, Mahmood Khan, Jianjun Guan
ACS APPLIED MATERIALS & INTERFACES
(2016)
Article
Engineering, Biomedical
Yanyi Xu, Minghuan Fu, Zhihong Li, Zhaobo Fan, Xiaofei Li, Ying Liu, Peter M. Anderson, Xiaoyun Xie, Zhenguo Liu, Jianjun Guan
ACTA BIOMATERIALIA
(2016)
Article
Oncology
Encheng Yang, Xiao Li, Ningyi Jin
CANCER CELL INTERNATIONAL
(2016)
Review
Cell & Tissue Engineering
Xiaofei Li, Kenichi Tamama, Xiaoyun Xie, Jianjun Guan
STEM CELLS INTERNATIONAL
(2016)
Article
Materials Science, Biomaterials
Qingxia Zhu, Xiaofei Li, Zhaobo Fan, Yanyi Xu, Hong Niu, Chao Li, Yu Dang, Zheng Huang, Yun Wang, Jianjun Guan
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2018)
Article
Engineering, Biomedical
Hong Niu, Xiaofei Li, Haichang Li, Zhaobo Fan, Jianjie Ma, Jianjun Guan
ACTA BIOMATERIALIA
(2019)
Article
Multidisciplinary Sciences
Xiaofei Li, Julie Braza, Ulrike Mende, Gaurav Choudhary, Peng Zhang
Summary: Our study found that early intervention with sacubitril/valsartan is superior to valsartan in reducing pressure overload-induced ventricular fibrosis and in reducing angiotensin II-induced adult ventricular fibroblast activation. While neither sacubitril/valsartan nor valsartan changes cardiac hypertrophy development, early intervention with sacubitril/valsartan protects ventricular myocytes from mitochondrial dysfunction and is superior to valsartan in reducing mitochondrial oxidative stress in response to persistent left ventricular pressure overload.
SCIENTIFIC REPORTS
(2021)
Article
Cardiac & Cardiovascular Systems
Nedyalka Valkov, Michelle E. King, Jacob Moeller, Hong Liu, Xiaofei Li, Peng Zhang
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2019)