Article
Immunology
Paula Alvarenga Borges, Ingrid Waclawiak, Janaina Lima Georgii, Vanderlei da Silva Fraga-Junior, Janaina Figueiredo Barros, Felipe Simoes Lemos, Thais Russo-Abrahao, Elvira Maria Saraiva, Christina M. Takiya, Robson Coutinho-Silva, Carmen Penido, Claudia Mermelstein, Jose Roberto Meyer-Fernandes, Fabio B. Canto, Josiane Sabbadini Neves, Paulo A. Melo, Claudio Canetti, Claudia Farias Benjamim
Summary: The study investigated the role of ADP in wound healing in diabetic patients, showing that ADP accelerated wound healing, improved new tissue formation, reduced ROS production, and increased the count of specific cell types in the wound. The effects of ADP were mainly mediated by P2Y(12) receptor activation, with the involvement of P2Y(1) receptor in the early stages of the repair process.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Laura D. Manzanares-Meza, Claudia Gutierrez-Roman, Albertana Jimenez-Pineda, Felipe Castro-Martinez, Genaro Patino-Lopez, Eunice Rodriguez-Arellano, Ricardo Valle-Rios, Vianney F. Ortiz-Navarrete, Oscar Medina-Contreras
Summary: Mucosal innate immunity is the first line of defense against pathogens. IL-1 cytokines are important regulators in inflamed mucosa. The secretion of IL-1 cytokines is regulated by unconventional pathways. Mouse macrophages experiments indicate IL-36 gamma is mainly secreted through membrane pores formed by P2X7R and Gasdermin D.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Pedro Vargas, Thamiris Becker Scheffel, Fernando Mendonca Diz, Liliana Rockenbach, Nathalia Grave, Angelica Regina Cappellari, Luiza Wilges Kist, Mauricio Reis Bogo, Marcos Paulo Thome, Gabriel Fernandes Leal, Amanda de Fraga Dias, Fabricio Figueiro, Eduardo Cremonese Filippi-Chiela, Guido Lenz, Fernanda Bueno Morrone
Summary: This study investigated the role of the P2Y(12) receptor in the proliferation and migration of GBM cells and evaluated its expression in patients' data. The results showed that the P2Y(12) antagonist, ticagrelor, decreased the viability and proliferation of GBM cells and reduced colony formation and migration potentials. The expression of P2Y(12)R was found to be higher in glioma samples, suggesting its importance in glioma development.
PURINERGIC SIGNALLING
(2022)
Article
Urology & Nephrology
M. A. Mawhin, R. G. Bright, J. D. Fourre, E. Vloumidi, J. Tomlinson, A. Sardini, C. D. Pusey, K. J. Woollard
Summary: This study reveals that decreased kidney function leads to the expansion of cardiac macrophages, particularly through local proliferation of resident populations, with circulating monocytes also contributing to this increase. CXCL10 is identified as a crucial factor for cardiac macrophage expansion in uremic disease. Increased plasma CXCL10 concentrations are found in advanced CKD patients, and production of CXCL10 is observed in cardiomyocytes and cardiac fibroblasts.
Article
Biochemistry & Molecular Biology
Dabin Lee, Dong Wook Kim, Sanghyuk Yoon, A-Reum Nam, Kang-Hoon Lee, Ki-Hoan Nam, Sang-Mi Cho, Yeodae Yoon, Je-Yoel Cho
Summary: The study demonstrated that CXCL5 plays a critical role in regulating energy metabolism by affecting the expression of proteins involved in fatty acid oxidation, mitochondrial localization, thermogenesis genes, and inflammatory genes. Additionally, CXCL5 promotes the expression of UCP1 in adipocytes, indicating its importance in energy metabolism regulation, particularly in response to cold exposure.
JOURNAL OF LIPID RESEARCH
(2021)
Article
Oncology
Tessa Skroblyn, Jara J. Joedicke, Madlen Pfau, Kerstin Krueger, Jean-Pierre Bourquin, Shai Izraeli, Cornelia Eckert, Uta E. Hoepken
Summary: The study identified CXCL12-CXCR4 as the leading signaling axis for B-ALL cell migration and survival in the testicular leukemic niche. CXCR4 was found to be the only chemokine receptor robustly expressed on B-ALL cells in both diagnosis and relapse samples. Blocking CXCR4 functions showed promising results in preventing testicular infiltration and overall development of leukemia.
JOURNAL OF PATHOLOGY
(2022)
Article
Physiology
Katja Koeppen, Amanda Nymon, Roxanna Barnaby, Zhongyou Li, Thomas H. Hampton, Alix Ashare, Bruce A. Stanton
Summary: Mutations in CFTR affect macrophage responses to bacteria, while EVs secreted by AEC play a regulatory role in macrophages, especially in the presence of Pseudomonas aeruginosa, with attenuated effects in CF macrophages.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Martin Schubert, Stefan Kluge, Elena Brunner, Simona Pace, Marc Birringer, Oliver Werz, Stefan Lorkowski
Summary: The study demonstrated that alpha-13'-COOH can suppress the production of inflammatory factors in macrophages and exert its anti-inflammatory effects by inhibiting the MAPK and NFxB pathways.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Emergency Medicine
Ahmed Jawhar, Desiree Brenner, Carolina de la Torre, Carsten Sticht, Udo Obertacke, Norbert Ponelies
Summary: This study found that tourniquet application during total knee arthroplasty significantly affects gene expression within vastus medialis cells, leading to changes in metabolism, genetic information processing, and cellular processes.
EUROPEAN JOURNAL OF TRAUMA AND EMERGENCY SURGERY
(2021)
Article
Cell Biology
Chen Cai, Timo Itzel, Haristi Gaitantzi, Carolina de la Torre, Emrullah Birgin, Johannes Betge, Norbert Gretz, Andreas Teufel, Nuh N. Rahbari, Matthias P. Ebert, Katja Breitkopf-Heinlein
Summary: The study revealed that BMP-9 can modulate the ratio between ID1 and noggin in colorectal cancer patients, significantly impacting patient survival rates. Experimental results showed that stimulation of organoids with BMP-9 promoted the expression of tumor suppressive genes, while noggin stimulation had the opposite effect.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Article
Plant Sciences
Monika Huber, Laura Armbruster, Ross D. Etherington, Carolina de la Torre, Malcolm J. Hawkesford, Carsten Sticht, Daniel J. Gibbs, Ruediger Hell, Markus Wirtz
Summary: The NatA and NatB complexes in Arabidopsis thaliana play critical roles in plant stress responses. NatA is involved in drought tolerance, while NatB is required for pathogen resistance and adaptation to high salinity and osmotic stress. NatB mutants are hypersensitive to DTT, suggesting that their hypersensitivity is caused by over-reduction of the cytosol.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Oncology
Rebekka Bitsch, Annina Kurzay, Feyza Oezbay Kurt, Carolina De la Torre, Samantha Lasser, Alisa Lepper, Alina Siebenmorgen, Verena Mueller, Peter Altevogt, Jochen Utikal, Viktor Umansky
Summary: STAT3 inhibitor Napabucasin can block the accumulation and activity of MDSC and improve the prognosis of patients with malignant melanoma. Patients with high expression of activated STAT3 in M-MDSC have shorter progression-free survival, indicating that STAT3 may be a promising therapeutic target in the treatment of malignant melanoma.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Correction
Oncology
Liping Bai, Tobias Pfeifer, Wolfgang Gross, Carolina De La Torre, Shuyang Zhao, Li Liu, Michael Schaefer, Ingrid Herr
FRONTIERS IN ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Pavlina Miklankova, Eric Linster, Jean-Baptiste Boyer, Jonas Weidenhausen, Johannes Mueller, Laura Armbruster, Karine Lapouge, Carolina De La Torre, Willy Bienvenut, Carsten Sticht, Matthias Mann, Thierry Meinnel, Irmgard Sinning, Carmela Giglione, Rudiger Hell, Markus Wirtz
Summary: This study identifies AtHYPK protein as the first in vivo regulator of NatA activity in plants, which interacts with the ribosome-anchoring subunit of NatA and promotes N-terminal acetylation of various substrates. Loss-of-AtHYPK mutants exhibit remarkable resistance to drought stress and resemble the phenotype of NatA-depleted plants. HYPK regulates plant metabolism and development by modulating NatA activity, and plays a critical role in global proteostasis.
Article
Medicine, Research & Experimental
Li Liu, XueFeng An, Michael Schaefer, Bin Yan, Carolina de la Torre, Stefan Hillmer, Jury Gladkich, Ingrid Herr
Summary: This study investigated the mechanisms by which silver nanoparticles inhibit pancreatic ductal adenocarcinoma (PDAC). The results showed that minimal amounts of silver nanoparticles selectively eradicated PDAC cells and inhibited cell migration, spheroid and colony formation. Furthermore, silver nanoparticles damaged mitochondria and induced paraptosis-like cell death. In addition, silver nanoparticles decreased tumor xenograft growth without side effects and reduced the expression of markers for proliferation and DNA repair, but upregulated paraptosis markers. This highlights the potential of silver nanoparticles as a complementary agent to improve the therapeutic efficacy in pancreatic cancer.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Cardiac & Cardiovascular Systems
Calin-Petru Manta, Thomas Leibing, Mirco Friedrich, Hendrik Nolte, Monica Adrian, Kai Schledzewski, Jessica Krzistetzko, Christof Kirkamm, Christian David Schmid, Yannick Xi, Ana Stojanovic, Sarah Tonack, Carolina de la Torre, Seddik Hammad, Stefan Offermanns, Marcus Krueger, Adelheid Cerwenka, Michael Platten, Sergij Goerdt, Cyrill Geraud
Summary: This study shows that inhibiting Stabilin-1 and Stabilin-2 can reduce the occurrence of atherosclerosis. By affecting the plasma proteome and the function of monocytes, this inhibition can suppress patrolling and inflammatory monocytes, providing systemic protection against atherosclerosis.
Article
Biochemistry & Molecular Biology
Cristina Ruberti, Elias Feitosa-Araujo, Zhaolong Xu, Stephan Wagner, Matteo Grenzi, Essam Darwish, Sophie Lichtenauer, Philippe Fuchs, Ambra Selene Parmagnani, Daria Balcerowicz, Sebastjen Schoenaers, Carolina de la Torre, Khansa Mekkaoui, Adriano Nunes-Nesi, Markus Wirtz, Kris Vissenberg, Olivier Van Aken, Bettina Hause, Alex Costa, Markus Schwarzlaender
Summary: The study demonstrates that MCU proteins mediate mitochondrial Ca2+ transport in plants, serving as the major route for fast Ca2+ uptake. Knockout of mcu genes limits mitochondrial Ca2+ uptake, leading to impaired subcellular Ca2+ transport and deregulated jasmonic acid-related signaling and thigmomorphogenesis.
Article
Oncology
Isabel Porth, Daniela Hirsch, Yonca Ceribas, Philip Weidner, Wilko Weichert, Thorsten Oliver Goetze, Sven Perner, Kim Luley, Christian Moritz Heyer, Carolina de la Torre, Ralf-Dieter Hofheinz, Sylvie Lorenzen, Timo Gaiser
Summary: This study aims to identify potential prognostic biomarkers for long-term response to trastuzumab maintenance therapy in HER2-positive metastatic gastric and gastroesophageal junction cancer patients. The results show that long-term responding patients have higher PD-L1 positivity scores, which are associated with longer progression-free survival. ERBB2 copy number and tumor mutational burden cannot discriminate between short-term and long-term responding patients.
EUROPEAN JOURNAL OF CANCER
(2023)
Article
Oncology
Bianca Dietsch, Celine Weller, Carsten Sticht, Carolina de la Torre, Martin Kramer, Sergij Goerdt, Cyrill Geraud, Sebastian A. Wohlfeil
Summary: This study focused on NRAS mutated melanoma and found that liver metastasis increased while lung metastasis decreased after hepatic passaging. Further analysis revealed that differential regulation of cell adhesion pathways might be the critical factor influencing the metastatic pattern.
Article
Biochemistry & Molecular Biology
George Rasti, Maximilian Becker, Berta N. Vazquez, Maria Espinosa-Alcantud, Irene Fernandez-Duran, Andres Gamez-Garcia, Alessandro Ianni, Jessica Gonzalez, Laia Bosch-Presegue, Anna Marazuela-Duque, Anna Guitart-Solanes, Sandra Segura-Bayona, Joan-Josep Bech-Serra, Michael Scher, Lourdes Serrano, Uma Shankavaram, Hediye Erdjument-Bromage, Paul Tempst, Danny Reinberg, Mireia Olivella, Travis H. Stracker, Carolina de la Torre, Alejandro Vaquero
Summary: SIRT1 plays a crucial role in regulating the DNA damage response by interacting with the PP4 phosphatase complex to control the signaling of DNA damage and repair.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Cell Biology
Seddik Hammad, Christoph Ogris, Amnah Othman, Pia Erdoesi, Wolfgang Schmidt-Heck, Ina Biermayer, Barbara Helm, Yan Gao, Weronika Pioronska, Christian H. H. Holland, Lorenza A. A. D'Alessandro, Carolina de la Torre, Carsten Sticht, Sherin Al Aoua, Fabian J. J. Theis, Heike Bantel, Matthias P. P. Ebert, Ursula Klingmueller, Jan G. G. Hengstler, Steven Dooley, Nikola S. S. Mueller
Summary: The human liver can regenerate to compensate for fibrosis caused by toxic chemicals, drugs, alcohol, or malnutrition. This study identified dysregulation of lipid metabolism and mild inflammation as protective mechanisms in liver fibrosis. The findings suggest that lipid metabolism and mild inflammation could be used as biomarkers and functional requirements to resist disease progression.
CELL DEATH & DISEASE
(2023)
Article
Medicine, Research & Experimental
Laura Planas-Serra, Nathalie Launay, Leire Goicoechea, Benedicte Heron, Cristina Jou, Natalia Julia-Palacios, Montserrat Ruiz, Stephane Fourcade, Carlos Casasnovas, Carolina De La Torre, Antoinette Gelot, Maria Marsal, Pablo Loza-Alvarez, Angels Garcia-Cazorla, Ali Fatemi, Isidre Ferrer, Manel Portero-Otin, Estela Area-Gomez, Aurora Pujol
Summary: Sphingolipids play important roles as membrane constituents and signaling molecules, and are crucial in human diseases, such as neurodevelopmental disorders and cancer. The deficiency in the DEGS1 enzyme, which functions in the sphingolipid pathway, is associated with hypomyelinating leukodystrophy-18 (HLD18). This study reveals that DEGS1 is a mitochondria-associated endoplasmic reticulum membrane-resident enzyme, and its deficiency disrupts core functions of the mitochondria-associated endoplasmic reticulum membrane, resulting in various dysfunctions related to mitochondrial dynamics, cholesterol metabolism, phospholipid metabolism, and lipid droplet biogenesis.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Meeting Abstract
Immunology
L. S. Silva Bermudez, T. Sevastyanova, C. De La Torre, L. Schumacher, H. Klueter, J. Kzhyshkowska
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)