Article
Biochemistry & Molecular Biology
Mette Heiskanen, Olli Jaaskelainen, Eppu Manninen, Shalini Das Gupta, Pedro Andrade, Robert Ciszek, Olli Grohn, Sanna-Kaisa Herukka, Noora Puhakka, Asla Pitkanen
Summary: Plasma neurofilament light chain (NF-L) levels are a diagnostic biomarker for traumatic brain injury (TBI) and a prognostic biomarker for somatomotor recovery and long-term structural brain damage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Rheumatology
Erika Huijser, Jens Gopfert, Zana Brkic, Cornelia G. van Helden-Meeuwsen, Sanne Jansen, Thomas Mandl, Peter Olsson, Benjamin Schrijver, Marco W. J. Schreurs, Paul L. A. van Daele, Willem A. Dik, Marjan A. Versnel
Summary: Type I IFN activation is a prominent feature of primary SS (pSS), SLE, and SSc, and ultrasensitive single-molecule array (Simoa) technology has facilitated the measurement of IFNs at subfemtomolar concentrations. In this study, IFN-alpha 2 levels in serum were measured in pSS, SLE, and SSc patients, correlating with blood IFN-stimulated gene expression and disease activity. The results showed that serum IFN-alpha 2 levels were elevated in pSS, positively correlated with ISG expression, and had potential for patient stratification or retrospective analysis of historical cohorts.
Review
Cell Biology
Anna Chanou, Stephan Hamperl
Summary: It has been discovered that eukaryotic chromatin is further packed through interactions with numerous protein complexes, providing a template for important biological processes such as DNA replication, transcription, and repair. Therefore, single-molecule approaches are essential for overcoming chromatin heterogeneity and monitoring chromatin transactions in real-time.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Veterinary Sciences
Taesik Yun, Yoonhoi Koo, Yeon Chae, Dohee Lee, Hakhyun Kim, Mhan-Pyo Yang, Byeong-Teck Kang
Summary: This study found a positive relationship between the rate of change in lesion size and the rate of change in serum NfL levels. In the decreased lesion size group, all dogs showed a decrease in NfL levels, while in the increased lesion size group, all dogs showed an increase in NfL levels. Therefore, serum NfL levels may be a promising biomarker for predicting lesion progression and resolution in MUO.
VETERINARY MEDICINE AND SCIENCE
(2023)
Article
Biochemical Research Methods
Qiyong Cai, Jingjing Mu, Yang Lei, Jia Ge, Aaron Albert Aryee, Xiaoge Zhang, Zhaohui Li
Summary: A new ultrasensitive, rapid, and duplex digital enzyme-linked immunosorbent assay (dELISA) has been developed for simultaneous detection of SARS-CoV-2 spike (S-RBD) and nucleocapsid (N) proteins based on a single molecule array, offering high sensitivity and specificity to improve the diagnosis accuracy of COVID-19.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Veterinary Sciences
Taesik Yun, Yoonhoi Koo, Yeon Chae, Dohee Lee, Hakhyun Kim, Soochong Kim, Dongwoo Chang, Ki-Jeong Na, Mhan-Pyo Yang, Byeong-Teck Kang
Summary: NfL concentrations were significantly higher in MUE dogs compared to healthy dogs, making it a potential biomarker for diagnosing MUE and evaluating treatment response.
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Yumei An, Sunao Li, Xinqi Huang, Xueshi Chen, Haiyan Shan, Mingyang Zhang
Summary: Copper plays an important role in various biological processes in the human body. Imbalance in copper homeostasis in the brain can lead to neurological pathological effects, suggesting a relationship between impaired copper homeostasis and brain diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Yu P. Zhang, Evgeniia Lobanova, Derya Emin, Sergey V. Lobanov, Antonina Kouli, Caroline H. Williams-Gray, David Klenerman
Summary: This study introduces a new method for the diagnosis of early Parkinson's disease. The results show that the proportion of soluble alpha-synuclein aggregates can distinguish between Parkinson's disease patients and control groups, with Parkinson's disease patients having a higher proportion of larger and rounder alpha-synuclein aggregates. By combining the number and morphology of the aggregates, a new biomarker with improved accuracy for early Parkinson's disease diagnosis was constructed.
ANALYTICAL CHEMISTRY
(2023)
Article
Neurosciences
Sarah E. Svirsky, Youming Li, Jeremy Henchir, Anna Rodina, Shaun W. Carlson, Gabriela Chiosis, C. Edward Dixon
Summary: This study investigates the formation of the epichaperome in traumatic brain injury (TBI) and suggests that this mechanism may contribute to protein misfolding, degradation, aggregation, and transport. Further understanding of this pathological mechanism can provide insights into the connection between TBI and increased risk of neurodegenerative diseases, as well as potential therapeutic targets.
NEUROBIOLOGY OF DISEASE
(2023)
Article
Pharmacology & Pharmacy
Dilorom Sass, Vivian A. Guedes, Ethan G. Smith, Rany Vorn, Christina Devoto, Katie A. Edwards, Sara Mithani, James Hentig, Chen Lai, Chelsea Wagner, Kerri Dunbar, David R. Hyde, Leorey Saligan, Michael J. Roy, Jessica Gill
Summary: This study found sex differences in TBI patients, with females showing higher levels of biomarkers and behavioral symptoms compared to males. The relationship between biomarkers and behavioral outcomes was more evident in females than males, suggesting a need for further research on individualized treatment guidelines.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Chemistry, Analytical
Hui Wang, Cuilin Zhu, Di Li
Summary: The emergence of single-molecule techniques has greatly advanced research in enzymology. Single-molecule enzymology studies the individual behavior of a single enzyme molecule, which is hidden under the overall average. This review summarizes the emerging developments of several single-molecule techniques, such as TIRFM, confocal microscopy, FCS, and magnetic/optical tweezers, in real-time monitoring of the catalytic process of single enzymes. It explores various enzymatic catalytic behaviors and mechanisms that differ from bulk experiments, providing a deeper understanding of enzymatic reaction mechanisms through the analysis of single-molecule enzymatic kinetics coupled with conformational changes. Additionally, this review critically discusses the future challenges of imaging and monitoring single enzymatic reactions in cellular environments.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Zhi Ruan, Qing Lu, Jennifer E. Wang, Mi Zhou, Shuiqiao Liu, Hongxia Zhang, Akshay Durvasula, Yijie Wang, Yanan Wang, Weibo Luo, Yingfei Wang
Summary: The study found that MIF mediates TBI-induced neurodegeneration, neuronal cell death, and neurobehavioral dysfunction through its nuclease activity, but not its pro-inflammatory role. Targeting MIF's nuclease activity may offer a novel strategy to protect neurons from TBI.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Clinical Neurology
William P. Flavin, Helia Hosseini, Jeffrey W. Ruberti, H. Pirouz Kavehpour, Christopher C. Giza, Mayumi L. Prins
Summary: This review summarizes the structure and functions of Tau protein, highlighting its associations with various diseases and conditions including diabetes, stroke, Alzheimer's disease, brain injury, aging, and drug use. It explores the role of Tau phosphorylation in disease progression and discusses the potential for therapeutic interventions to prevent or reverse tau-mediated neurodegeneration.
FRONTIERS IN NEUROLOGY
(2023)
Article
Neurosciences
Daniel J. Brennan, Jeffrey Duda, Jeffrey B. Ware, John Whyte, Joon Yul Choi, James Gugger, Kristen Focht, Alexa E. Walter, Tamara Bushnik, James C. Gee, Ramon Diaz-Arrastia, Junghoon J. Kim
Summary: Traumatic brain injury (TBI) results in progressive neurodegeneration and brain atrophy. This study investigated the spatial and temporal evolution of TBI-related atrophy using longitudinal scans of 37 individuals with TBI and 33 matched controls. The results showed early cortical thinning and reduced volume in specific brain regions, as well as continued atrophy over time. The findings suggest that atrophy during the first year of TBI can serve as a biomarker for neurodegeneration.
HUMAN BRAIN MAPPING
(2023)
Review
Biochemistry & Molecular Biology
Rafael Carecho, Diogo Carregosa, Bernardo Oliveira Ratilal, Ines Figueira, Maria Angeles Avila-Galvez, Claudia Nunes dos Santos, Natasa Loncarevic-Vasiljkovic
Summary: Traumatic brain injury (TBI) is a major cause of death and disability in young adults worldwide. The underlying mechanisms of TBI are still not fully understood, despite advances in our knowledge. Secondary brain injury processes occur gradually following the initial acute damage, providing a therapeutic window for interventions. Nutritional interventions, particularly (poly)phenols found in fruits and vegetables, have shown promise in enhancing the repair processes after TBI. However, more research is needed to fully understand the effects and limitations of (poly)phenols in TBI.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nursing
Shirley M. Moore, Carol M. Musil, Anthony I. Jack, Megan L. Alder, David M. Fresco, Allison Webel, Kathy D. Wright, Abdus Sattar, Patricia Higgins
Article
Clinical Neurology
Grant C. O'Connell, Phillip Stafford, Kyle B. Walsh, Opeolu Adeoye, Taura L. Barr
Article
Clinical Neurology
Grant C. O'Connell, Megan L. Alder, Christine G. Smothers, Carolyn H. Still, Allison R. Webel, Shirley M. Moore
NEUROLOGICAL RESEARCH
(2020)
Article
Clinical Neurology
Megan L. Alder, Fei Ye, Fan Run, Kanika Bagai, Diane B. Fawkes, Barry T. Peterson, Beth A. Malow
Article
Nursing
Shirley M. Moore, Carol M. Musil, Megan L. Alder, Grant Pignatiello, Patricia Higgins, Allison Webel, Kathy D. Wright
Article
Neurosciences
Grant C. O'Connell, Christine G. Smothers, Chris Winkelman
Article
Neurosciences
Grant C. O'Connell, Megan L. Alder, Christine G. Smothers, Carolyn H. Still, Allison R. Webel, Shirley M. Moore
Article
Multidisciplinary Sciences
Grant C. O'Connell, Megan L. Alder, Christine G. Smothers, Julia H. C. Chang
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Nursing
Kelly L. Wierenga, David M. Fresco, Megan Alder, Shirley M. Moore
Summary: The study aimed to evaluate a new education program (RENEwS) designed to enhance emotion regulation strategies among cardiac rehabilitation patients. Through the participation of 14 cardiac rehabilitation patients with an average age of 61 in a five-week session, the research found that the majority of participants viewed the intervention as feasible, with multiple strengths identified.
WESTERN JOURNAL OF NURSING RESEARCH
(2021)
Article
Psychology, Developmental
M. L. Alder, C. R. Johnson, J. A. Zauszniewski, B. A. Malow, C. J. Burant, L. Scahill
Summary: This study evaluated the feasibility of using actigraphy to measure sleep and physical activity in children with ASD, and found associations between sleep and physical activity.
JOURNAL OF AUTISM AND DEVELOPMENTAL DISORDERS
(2023)
Article
Health Care Sciences & Services
Megan L. Alder, Carolyn H. Still, Kelly L. Wierenga, Grant A. Pignatiello, Shirley M. Moore
Summary: This study assessed the differences in algorithms used to determine wear time and physical activity in chronic illness populations. The findings suggest that the choice of algorithm does make a difference in physical activity determination, particularly in light physical activity, metabolic equivalents, and moderate-to-vigorous physical activity.
