Construction of a restriction-less, marker-less mutant useful for functional genomic and metabolic engineering of the biofuel producer Clostridium acetobutylicum

Coenzyme A-transferase-independent butyrate re-assimilation in Clostridium acetobutylicum—evidence from a mathematical model

(2014) Thomas Millat et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Novel System for Efficient Isolation of Clostridium Double-Crossover Allelic Exchange Mutants Enabling Markerless Chromosomal Gene Deletions and DNA Integration

(2012) Mohab A. Al-Hinai et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Targeted mutagenesis of the Clostridium acetobutylicum acetone–butanol–ethanol fermentation pathway

(2012) Clare M. Cooksley et al.   METABOLIC ENGINEERING

Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker

(2012) John T. Heap et al.   NUCLEIC ACIDS RESEARCH

Regulation of transcription by unnatural amino acids

(2011) Chang C Liu et al.   NATURE BIOTECHNOLOGY

Metabolic engineering of the non-sporulating, non-solventogenic Clostridium acetobutylicum strain M5 to produce butanol without acetone demonstrate the robustness of the acid-formation pathways and the importance of the electron balance

(2008) Ryan Sillers et al.   METABOLIC ENGINEERING