Article
Medicine, Research & Experimental
Mei-li Wei, Shi-min He, An-qi Chen, Zi-xuan Fan, Wei Liu, Ling Zhang, Shi-jie Lv, Cheng-zeng Qiu, Hui-ru Liu, Mu-xian Hao, Zong-jun Yin, Da-long Ren
Summary: This study investigated the effects of FLX on circadian rhythm using zebrafish as a model. The results showed that FLX treatment led to down-regulation of clock genes expression, reduction in amplitude and phase shift of circadian rhythms, as well as inhibition of melatonin secretion. These findings provide valuable insights into the impact of FLX exposure on circadian rhythm and locomotor activity, and may have implications for its clinical application.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Medicine, Research & Experimental
Candice Bohaud, Jholy De La Cruz, Claudia Terraza, Audrey Barthelaix, Beryl Laplace-Builhe, Christian Jorgensen, Yoan Arribat, Farida Djouad
Summary: This study using zebrafish model and caudal fin resection investigated the crucial role of lactate in macrophage response and fin regeneration. The findings suggest that lactate plays a key role in activating and modulating macrophage phenotype, promoting inflammatory response and tissue regeneration.
Article
Environmental Sciences
Jierong Chen, Jie Zhou, Minjia Li, Kun Zhang, Jiayin Dai, Yanbin Zhao
Summary: Circadian rhythm aligns various biological functions in animals, and can be regulated by synthetic substances. In this study, a high-throughput screening approach using zebrafish larvae was established to analyze the effects of synthetic substances on circadian rhythm. The study identified several circadian-disrupting compounds and demonstrated the feasibility of this screening strategy.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Ophthalmology
Jingxin He, Xinwei Jiao, Xin Sun, Yijia Huang, Pengyang Xu, Yunxia Xue, Ting Fu, Jun Liu, Zhijie Li
Summary: The study showed that high fructose intake significantly reprograms the circadian rhythms of the cornea in mice, altering cell cycle, immune function, and neuronal-related pathways. HFI affected the distribution and number of corneal epithelial mitotic cells, and increased the recruitment of immune cells to the limbal region over a circadian cycle.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Han Hu, Yong Long, Guili Song, Shaoxiong Chen, Zhicheng Xu, Qing Li, Zhengli Wu
Summary: This study reveals the crucial roles of protein kinase C alpha (PKC alpha/PRKCA) in modulation of animal social behavior and circadian rhythm. The deficiency of PRKCA leads to anxiety-like behavior and impaired social preference in zebrafish, which are associated with the disturbed circadian rhythm. These findings provide new insights into the understanding of PRKCA's function in animal social interactions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Yuling Ding, Xing Dong, Weiwei Feng, Guanghua Mao, Yao Chen, Xuchun Qiu, Kun Chen, Hai Xu
Summary: The study revealed significant impacts of TBBPS exposure on the development and circadian rhythm gene expression in zebrafish larvae, potentially leading to alterations in the circadian rhythm network and abnormal behavioral responses.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Xiaochao Qu, Mei Liao, Weiwei Liu, Yisheng Cai, Qiaorong Yi, Jianmei Long, Lijun Tan, Yun Deng, Hongwen Deng, Xiangding Chen
Summary: This study successfully generated a wnt16(-/-) zebrafish model using CRISPR-Cas9 technology, and found that the wnt16 gene plays a crucial role in skeletal development. Through RNA sequencing and qRT-PCR validation, a series of down-regulated DEGs were identified, revealing the molecular mechanisms by which wnt16 regulates bone metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Environmental Sciences
Emma B. Meade, Luke R. Iwanowicz, Nicklaus Neureuther, Gregory H. LeFevre, Dana W. Kolpin, Hui Zhi, Shannon M. Meppelink, Rachael F. Lane, Angela Schmoldt, Aurash Mohaimani, Olaf Mueller, Rebecca D. Klaper
Summary: This study investigates the biological impacts and biomarkers of wastewater treatment plant (WWTP) effluent exposure on zebrafish embryos through transcriptomics analysis. The results reveal that WWTP effluent not only affects traditional markers of endocrine disruption but also impacts a wide range of biological functions and pathways, including cardiac, neurological, visual, metabolic, and signaling pathways.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Fan Wang, Fei Liu
Summary: This study investigated the potential effects of Triclosan (TCS) on cellular targets in zebrafish larvae. The results showed that TCS potentially affected reproduction, immune, and metabolism processes in zebrafish larvae.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2023)
Article
Environmental Sciences
Shixuan Zhang, Luli Wu, Junrou Zhang, Xueting Wang, Xin Yang, Ye Xin, Li Chen, Jie Li, Piye Niu
Summary: This study utilized single-cell RNA sequencing to identify 10 cell types in zebrafish brain and revealed the critical role of dopamine neurons in manganese-induced neurological damage. Long-term manganese exposure impaired amino acid and lipid metabolic processes in the brain and disrupted the ferroptosis signaling pathway in zebrafish dopamine neurons. This study provided insights into a novel potential mechanism of manganese neurotoxicity.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Integrative & Complementary Medicine
Rong-rong Wang, Ting Li, Lei Zhang, Zheng-yan Hu, Li Zhou, Le-tian Shan, Jia-wei Huang, Lan Li
Summary: By conducting toxicological and RNA sequencing assays on zebrafish larvae, the toxicity of Panax notoginseng and its potential developmental toxicity were evaluated. The results showed that dPN exhibited developmental toxicity by decreasing the body weight, body length, and vertebral number of zebrafish larvae. The RNA sequencing data identified functional genes involved in regulating body development of zebrafish.
CHINESE JOURNAL OF INTEGRATIVE MEDICINE
(2023)
Article
Environmental Sciences
Ying Han, Yuanyuan Ma, Junwei Tong, Jingpu Zhang, Changqin Hu
Summary: Statins have toxic effects on the development of zebrafish embryos, causing developmental defects. They also alter the expression of genes related to heart contraction, transcription factors, and other functions. Through network analysis, certain genes were identified as potential toxicity targets of statins. In silico prediction suggests that statins pose a certain risk to aquatic organisms.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Engineering, Environmental
Le Qian, Suzhen Qi, Zhao Wang, Jason T. Magnuson, David C. Volz, Daniel Schlenk, Jiazhen Jiang, Chengju Wang
Summary: The study evaluated the effects of environmentally relevant boscalid concentrations on zebrafish, indicating that boscalid significantly impacted the behavior and physiology of both larval and adult zebrafish. Predation rates were decreased, and adverse alterations in eye and brain cell differentiation were observed after exposure to boscalid. Gene expression analysis suggested that boscalid may impair visual and nervous system function in zebrafish.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Environmental Sciences
Zuo Wang, Kemin Li, Yanyi Xu, Zan Song, Xianyong Lan, Chuanying Pan, Shengxiang Zhang, Nicholas S. Foulkes, Haiyu Zhao
Summary: Nickel (Ni) is a widely used heavy metal that can cause environmental pollution and health hazards. The neurotoxic effects induced by exposure to environmentally relevant levels of Ni in zebrafish larvae are closely related to ferroptosis, a form of iron-mediated cell death. The activation of the ferroptosis pathway and iron dyshomeostasis are implicated in these Ni-induced neurotoxic effects.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Physiology
Molly A. Bingham, Kim Neijman, Chin -Rang Yang, Angel Aponte, Angela Mak, Hiroaki Kikuchi, Hyun Jun Jung, Brian G. Poll, Viswanathan Raghuram, Euijung Park, Chung -Lin Chou, Lihe Chen, Jens Leipziger, Mark A. Knepper, Margo Dona
Summary: The circadian variability in kidney function is often ignored in physiological experiments. This study used transcriptomics and proteomics to identify genes and proteins in the kidney that show circadian rhythms. These data have been made available as user-friendly web resources.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ana Fiszbein, Keegan S. Krick, Bridget E. Begg, Christopher B. Burge
Article
Biochemistry & Molecular Biology
William E. Robinson, William E. Robinson, Keegan Krick, Harry M. Murray, Helen C. Poynton
Summary: Mollusks, especially bivalves, exhibit diverse sex determining mechanisms, but these mechanisms are not well studied. In this study, researchers used phylotranscriptomics to identify candidate sex differentiating genes in eight bivalve species. By analyzing gene expression data and confirming the identity of these genes using phylogenetics analyses, they identified a Mollusk-specific sex determining gene and suggested key genes for male and female development.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2022)
Article
Multidisciplinary Sciences
Delaney K. Fischer, Keegan S. Krick, Chloe Han, Morgan T. Woolf, Elizabeth A. Heller
Summary: This study reveals the epigenetic regulation of the Nr4a1 gene in the reward brain regions of male and female mice following cocaine administration. It sheds light on the biological significance of sex in cocaine use disorder.
SCIENTIFIC REPORTS
(2022)
Article
Neurosciences
Akanksha Bhatnagar, Keegan Krick, Bhanu Chandra Karisetty, Ellen M. Armour, Elizabeth A. Heller, Felice Elefant
Summary: The severity of Alzheimer's disease (AD) progression involves complex interactions between genetics, age, and environmental factors regulated by neuroepigenetic mechanisms. This study reveals a new RNA binding function for Tip60 in addition to its known histone acetyltransferase (HAT) function. The study shows that Tip60 interacts with pre-mRNAs from chromatin neural gene targets and this RNA binding function is disrupted in AD models and patient hippocampus. Furthermore, Tip60's RNA targeting modulates splicing decisions, and splicing defects associated with AD pathology may be caused by disruption of Tip60's splicing function.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Cell Biology
Laura M. Agosto, Michael J. Mallory, Max B. Ferretti, Davia Blake, Keegan S. Krick, Matthew R. Gazzara, Benjamin A. Garcia, Kristen W. Lynch
Summary: Chromatin regulation and alternative splicing are two important mechanisms in gene expression. Histone modifications can affect alternative splicing decisions, but the impact of alternative splicing on chromatin is not well understood. This study found that several genes encoding histone-modifying enzymes, including HDAC7, are alternatively spliced in T cells. By manipulating the splicing of HDAC7 exon 9, the study showed changes in histone modifications and gene expression, with the long isoform induced by the RNA-binding protein CELF2 promoting the expression of critical T cell surface proteins. This demonstrates that alternative splicing of HDAC7 has a global impact on histone modification and gene expression in T cell development.
Article
Genetics & Heredity
Neelakanteswar Aluru, Sibel Karchner, Keegan S. Krick, Wei Zhu, Jiang Liu
ENVIRONMENTAL EPIGENETICS
(2018)