Article
Agriculture, Dairy & Animal Science
Kinga Orzechowska, Marta Kiezun, Edyta Rytelewska, Marlena Gudelska, Katarzyna Kisielewska, Grzegorz Kopij, Kamil Dobrzyn, Ewa Zaobidna, Karolina Szymanska, Tadeusz Kaminski, Nina Smolinska
Summary: This study aimed to evaluate the effects of orexin A (OXA) on mRNA abundance of enzymes involved in prostaglandin production and the concentrations of prostaglandin E2 (PGE2) and F-2 alpha (PGF(2 alpha)) in endometrial and myometrial explants. The results suggest that OXA may affect the synthesis and secretion of PGE2 and PGF(2 alpha) in the uterus by regulating the mRNA levels of enzymes involved in prostaglandin production.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Anthony Markham
Summary: Daridorexant, an orally administered dual orexin receptor antagonist, has been approved in the USA for the treatment of insomnia based on the results of pivotal phase III trials.
Article
Pharmacology & Pharmacy
Francisco J. Flores-Ramirez, Florence P. Varodayan, Reesha R. Patel, Jessica M. Illenberger, Francesca Di Ottavio, Marisa Roberto, Remi Martin-Fardon
Summary: Chronic alcohol use dysregulates stress responsivity, making individuals vulnerable to relapse. This study found that the OX1/2 receptor in the infralimbic cortex plays a significant role in stress-induced reinstatement of alcohol-seeking behavior. Using the OX1/2 antagonist TCS 1102 can prevent reinstatement in alcohol-dependent animals.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Barbara Jana, Jaroslaw Calka, Bartosz Micinski
Summary: Inflammation reduces GALR1 protein expression in pig myometrium, while GALR1 and GALR2 participate in the contractile regulation of an inflamed uterus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Hiroshi Tsuneki, Masanori Sugiyama, Kiyofumi Sato, Hisakatsu Ito, Sanaka Nagai, Kanta Kon, Tsutomu Wada, Nao Kobayashi, Takuya Okada, Naoki Toyooka, Masashi Kawasaki, Toshihiro Ito, Ryota Otsubo, Daisuke Okuzaki, Teruhito Yasui, Toshiyasu Sasaoka
Summary: The drug lemborexant, used to treat insomnia, has been found to have a positive effect on sleep and glucose metabolism in individuals with type 2 diabetes. In experiments, lemborexant increased sleep duration and improved glucose tolerance in db/db mice. This improvement did not affect body weight or insulin secretion.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Behavioral Sciences
Lu Wang, Yundan Pan, Chunyan Ye, Lizhe Guo, Sumei Luo, Sisi Dai, Na Chen, E. Wang
Summary: Orexin receptor antagonists can be widely used in adults and the elderly, with both short-term and long-term use being effective for primary insomnia. H1 antagonists are more effective in adults than in the elderly. Although benzodiazepines have a more obvious effect on sleep maintenance, it is best to reduce their use due to their side effects, especially for the elderly. As a food supplement, melatonin has little effect on adults, but it still has a certain effect on the elderly.
NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS
(2021)
Article
Cell Biology
Kinga Orzechowska, Kamil Dobrzyn, Marta Kiezun, Agata Malinowska, Bianka Swiderska, Tadeusz Kaminski, Nina Smolinska
Summary: This study examined the effects of CHEM on differentially regulated proteins in porcine endometrial tissue during implantation. It was found that CHEM modulates protein expressions involved in endometrial cell growth, migration, immune response, sensitivity to P4, and formation of new blood vessels. CHEM also appears to play a role in endothelial cell dysfunction during preeclampsia.
Article
Reproductive Biology
Hwanhee Jang, Soohyung Lee, Inkyu Yoo, Yohan Choi, Jisoo Han, Yugyeong Cheon, Hakhyun Ka
Summary: S100A8, S100A9, and S100A12, calcium-binding proteins, are dynamically expressed in response to conceptus-derived signals at the maternal-conceptus interface, potentially playing a critical role in regulating endometrial epithelial cell function and conceptus implantation to support the establishment and maintenance of pregnancy in pigs.
BIOLOGY OF REPRODUCTION
(2022)
Article
Chemistry, Multidisciplinary
Mohammed H. Alqarni, Muzaffar Iqbal, Ahmed I. Foudah, Tariq M. Aljarba, Fatma Abdel Bar, Sultan Alshehri, Faiyaz Shakeel, Prawez Alam
Summary: Suvorexant is a new sedative/hypnotic medication, and this study proposed and validated a sensitive and rapid HPTLC bioanalytical method for determining its concentration in human urine samples.
Review
Clinical Neurology
Maria P. Mogavero, Alessandro Silvani, Giuseppe Lanza, Lourdes M. DelRosso, Luigi Ferini-Strambi, Raffaele Ferri
Summary: After describing orexins and their roles in sleep and medical disorders, the article discusses the clinical evidence on the effects of DORAs and SORAs on insomnia to provide recommendations for further assessment in personalized and precision medicine. Trials with orexin receptor antagonists have shown potential as a valid therapeutic option for insomnia in various medical conditions, but more studies are needed to determine the most suitable options for different patient populations.
NATURE AND SCIENCE OF SLEEP
(2023)
Editorial Material
Biochemistry & Molecular Biology
Jane Y. Chen, Richard D. Palmiter
Summary: In this study, Xie et al. have identified a gut-to-brain pathway that triggers retching after toxic food ingestion or emetic agent administration. Their findings provide insights into how peripheral signals reach the brain to coordinate behavioral responses and facilitate learning to prevent repeated ingestion of harmful substances.
Article
Agriculture, Dairy & Animal Science
Peifang Yang, Xiang Chen, Xingzhou Tian, Zhinan Zhou, Yan Zhang, Wen Tang, Kaibin Fu, Jiafu Zhao, Yong Ruan
Summary: This study investigated the effect of N-acetyl-L-cysteine (NAC) supplementation on the expression of uterine keratin gene and protein in goats during early pregnancy. The results showed significant differences in uterine keratin expression and increases in antioxidant indices and hormone levels in the serum of goats. These findings provide new insight into the mechanism by which NAC regulates early pregnancy and embryonic development in goats.
Article
Psychiatry
Masahiro Takeshima, Yumi Aoki, Kenya Ie, Eiichi Katsumoto, Eichi Tsuru, Takashi Tsuboi, Ken Inada, Morito Kise, Koichiro Watanabe, Kazuo Mishima, Yoshikazu Takaesu
Summary: This study investigated the prescribing habits and reasons for selection of hypnotics among 962 physicians. The results showed that the most frequently prescribed hypnotic was an orexin receptor antagonist (ORA), followed by non-benzodiazepines, melatonin receptor agonists (MRA), and benzodiazepines. Physicians who frequently prescribed ORA were more concerned with efficacy and safety, while those who frequently prescribed MRA and non-benzodiazepines were more concerned with safety, and those who frequently prescribed benzodiazepines were more concerned with efficacy but less concerned with safety.
FRONTIERS IN PSYCHIATRY
(2023)
Review
Clinical Neurology
Chiara Berteotti, Carmen Calvello, Claudio Liguori
Summary: Epilepsy is a common neurological disorder that affects patients of all ages and is associated with reduced quality of life and comorbidities. Sleep impairment is frequently observed in epilepsy patients, and the relationship between sleep and epilepsy is bidirectional. The orexin system, which plays a role in controlling the sleep-wake cycle, may be affected in epilepsy patients. Clinical studies have shown inconsistent results due to different methodologies in quantifying orexin levels. Dual orexin receptor antagonists (DORAs) have been suggested as a potential treatment for sleep impairment and insomnia in epilepsy patients.
Article
Environmental Sciences
Yan Zhang, Linchao Zhang, Jialu Bao, Liantao Liu, Xiaodan Wang
Summary: The study revealed that PFOA can increase liver weight, reduce uterine index, and induce toxic effects on liver and uterine tissues. Additionally, various factors expressed in uterine cells showed dose-dependent changes in response to PFOA exposure.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
Kinga Orzechowska, Marta Kiezun, Edyta Rytelewska, Marlena Gudelska, Katarzyna Kisielewska, Grzegorz Kopij, Kamil Dobrzyn, Ewa Zaobidna, Karolina Szymanska, Tadeusz Kaminski, Nina Smolinska
Summary: This study aimed to evaluate the effects of orexin A (OXA) on mRNA abundance of enzymes involved in prostaglandin production and the concentrations of prostaglandin E2 (PGE2) and F-2 alpha (PGF(2 alpha)) in endometrial and myometrial explants. The results suggest that OXA may affect the synthesis and secretion of PGE2 and PGF(2 alpha) in the uterus by regulating the mRNA levels of enzymes involved in prostaglandin production.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Marlena Gudelska, Kamil Dobrzyn, Marta Kiezun, Katarzyna Kisielewska, Edyta Rytelewska, Tadeusz Kaminski, Nina Smolinska
Summary: This study found that Chemerin can affect steroid hormone synthesis in the porcine endometrium, particularly P-4 and E-2. Chemerin achieves this impact by regulating the expression levels of specific steroidogenic enzyme proteins at different stages of pregnancy and the estrous cycle. Additionally, Chemerin also influences hormone synthesis through the Erk and Akt signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Karol G. Makowczenko, Jan P. Jastrzebski, Lukasz Paukszto, Kamil Dobrzyn, Marta Kiezun, Nina Smolinska, Tadeusz Kaminski
Summary: Chemerin participates in the regulation of physiological and disorder mechanisms in mammals. This study investigates the influence of Chemerin on alternative mRNA transcription in porcine luteal cells, including differential expression of long non-coding RNAs (DELs) and their interactions with differentially expressed genes (DEGs), differences in alternative splicing of transcripts (DASs), and allele-specific expression (ASEs). The results provide new insights into the effects of Chemerin on the functions of the corpus luteum.
Article
Reproductive Biology
Kinga Orzechowska, Grzegorz Kopij, Lukasz Paukszto, Kamil Dobrzyn, Marta Kiezun, Jan Jastrzebski, Tadeusz Kaminski, Nina Smolinska
Summary: This study found that CHEM affects the gene expression profile of the porcine endometrium, especially genes related to cell migration and adhesion, angiogenesis, inflammation, and steroidogenesis. This suggests that CHEM plays an important role in the pig embryo implantation process.
BIOLOGY OF REPRODUCTION
(2022)
Article
Cell Biology
Kinga Orzechowska, Kamil Dobrzyn, Marta Kiezun, Agata Malinowska, Bianka Swiderska, Tadeusz Kaminski, Nina Smolinska
Summary: This study examined the effects of CHEM on differentially regulated proteins in porcine endometrial tissue during implantation. It was found that CHEM modulates protein expressions involved in endometrial cell growth, migration, immune response, sensitivity to P4, and formation of new blood vessels. CHEM also appears to play a role in endothelial cell dysfunction during preeclampsia.
Article
Cell Biology
Ewa Mlyczynska, Marta Kiezun, Patrycja Kurowska, Monika Dawid, Karolina Pich, Natalia Respekta, Mathilde Daudon, Edyta Rytelewska, Kamil Dobrzyn, Barbara Kaminska, Tadeusz Kaminski, Nina Smolinska, Joelle Dupont, Agnieszka Rak
Summary: In this review, we summarize the current knowledge about the expression and role of adipokines and neuropeptides in the physiological regulation of corpus luteum function, as well as their potential involvement in pathologies affecting the luteal cells that disrupt the estrous cycle.
Article
Reproductive Biology
Marlena Gudelska, Kamil Dobrzyn, Marta Kiezun, Katarzyna Kisielewska, Edyta Rytelewska, Tadeusz Kaminski, Nina Smolinska
Summary: This study aimed to investigate the influence of steroid hormones on the expression of the chemerin system in the uterus of domestic pigs. The results showed that both estradiol and progesterone can modulate the expression of chemerin receptor genes and proteins, as well as the abundance of RARRES2 mRNA transcripts. Additionally, estradiol increased chemerin protein secretion during the maternal recognition of pregnancy and decreased its release during embryo implantation.
Article
Biochemistry & Molecular Biology
Jacek Kiezun, Marta Kiezun, Bartlomiej Emil Krazinski, Lukasz Paukszto, Anna Koprowicz-Wielguszewska, Zbigniew Kmiec, Janusz Godlewski
Summary: This study found that the expression levels of GALR1 and GALR3 proteins were elevated in colorectal cancer tissue, and higher GALR3 expression was associated with better prognosis. The expression of GALR1 and GALR3 slightly decreased in myenteric plexuses close to the cancer, but showed no correlation with tumor progression. The expression levels of GALRs in submucosal plexuses did not significantly change between distal and close to the tumor. However, elevated GALR1 expression in submucosal plexuses close to the tumor correlated with poor prognosis, higher tumor grading, and shorter overall survival.
Article
Biochemistry & Molecular Biology
Karol G. Makowczenko, Jan P. Jastrzebski, Marta Kiezun, Lukasz Paukszto, Kamil Dobrzyn, Nina Smolinska, Tadeusz Kaminski
Summary: The aim of this study was to investigate the transcriptomic changes and mechanisms accompanying transcription and translation processes in the pituitary of the domestic pig. Through high-throughput sequencing of RNA, the study obtained information on gene expression changes, alternative splicing events, allele-specific expression sites, and RNA editing events. The study also confirmed the expression profiles of select phenomena and identified intracellular pathways that induce changes in transcription and translation regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Marta Kiezun, Kamil Dobrzyn, Marlena Gudelska, Edyta Rytelewska, Katarzyna Kisielewska, Ewa Zaobidna, Kinga Bors, Grzegorz Kopij, Karolina Szymanska, Barbara Kaminska, Tadeusz Kaminski, Nina Smolinska
Summary: The aim of this study was to evaluate the effect of prostaglandins on the expression of the orexin system in porcine uterine tissues. The results showed that prostaglandins play a regulatory role in the expression of the orexin system, suggesting a local mechanism for modulating uterine metabolism. The observed differences in the effects of prostaglandins may be attributed to changes in tissue sensitivity, the phase of the estrous cycle, the stage of early pregnancy, or the concentration of prostaglandins.
ANNALS OF ANIMAL SCIENCE
(2022)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.