Journal
BIOTECHNIQUES
Volume 60, Issue 3, Pages 137-140Publisher
BIOTECHNIQUES OFFICE
DOI: 10.2144/000114390
Keywords
3 '-end processing; reporter assay; gain-of-function
Funding
- Muscular Dystrophy Foundation (MDA) [202141]
- Friends of FSH Research
- Schissler Foundation Fellowship for Translational Studies of Common Human Diseases
Ask authors/readers for more resources
The concept of mRNA 3'-end formation as a static, minimally regulated housekeeping process has undergone a paradigm shift. Many recent studies have shown that accurate and efficient 3'-end formation of mRNA is highly regulated and that dysregulation of this process is a hallmark of several diseases. While there are many global analysis methods for monitoring altered mRNA processing, methods for investigating specific RNA 3'-end processing events in cells have not significantly changed. Here we describe a facile gain-of-function cellular reporter for the analysis of mRNA 3'-end formation as an alternative to approaches that are technically challenging or use radioactivity. We also offer suggestions for optimization of our approach and enhancement of its reproducibility.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available